FastQCFastQC Report
Fri 17 Jun 2016
SRR1528024_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528024_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11236418
Sequences flagged as poor quality0
Sequence length59
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA307.016042E-753.021
TCGACAA142450.050.656022
GACAATA2539800.050.254844
TAGGGGC1654300.050.2079129
TGACAAT863400.050.167073
CAACAAT709550.049.963643
TTACAAT1486750.049.8986053
ATAGGGG7765800.049.8814138
GAACAAT493900.049.8612063
TAGGGAT495550.049.8609629
AACAATA2819500.049.8213164
AATAGGG11660650.049.8063167
CAATAGG11821850.049.7985346
ACAATAG11905600.049.7921145
TTAACAA377500.049.791922
CGAACAA102300.049.7878762
TAACAAT956700.049.6899263
CACAATA2801650.049.627024
TAGGGGA1830200.049.597379
ATAGGGC800700.049.5547038
Produced by FastQC (version 0.11.5)