##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528024_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11236418
Sequences flagged as poor quality	0
Sequence length	59
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36007070936663	31.0	31.0	34.0	30.0	34.0
2	31.937149899549837	33.0	31.0	34.0	30.0	34.0
3	32.18115087922148	34.0	31.0	34.0	30.0	34.0
4	35.82050418558654	37.0	35.0	37.0	35.0	37.0
5	35.74651477009844	37.0	35.0	37.0	35.0	37.0
6	35.914704045363926	37.0	35.0	37.0	35.0	37.0
7	35.82998336302548	37.0	35.0	37.0	35.0	37.0
8	35.87711083727928	37.0	35.0	37.0	35.0	37.0
9	37.77753132715426	39.0	37.0	39.0	35.0	39.0
10	37.419358998570544	39.0	37.0	39.0	34.0	39.0
11	37.223231371421036	39.0	37.0	39.0	34.0	39.0
12	37.599346784713774	39.0	37.0	39.0	35.0	39.0
13	37.710864440963306	39.0	37.0	39.0	35.0	39.0
14	38.111031914263066	40.0	38.0	40.0	34.0	40.0
15	37.603486538147656	40.0	37.0	40.0	33.0	40.0
16	36.79582238752599	39.0	35.0	40.0	32.0	40.0
17	36.686104059140554	39.0	35.0	40.0	31.0	40.0
18	36.58230861472045	39.0	35.0	40.0	31.0	40.0
19	36.74596530673743	39.0	35.0	40.0	31.0	40.0
20	36.84391431504239	39.0	35.0	40.0	31.0	40.0
21	36.75892735567509	39.0	35.0	40.0	31.0	40.0
22	36.74449134946742	39.0	35.0	40.0	31.0	40.0
23	36.723853544786245	39.0	35.0	40.0	31.0	40.0
24	36.707925782041926	39.0	35.0	40.0	31.0	40.0
25	36.66922519258362	39.0	35.0	40.0	31.0	40.0
26	36.54341766210548	39.0	35.0	40.0	31.0	40.0
27	36.468191731564275	39.0	35.0	40.0	30.0	40.0
28	36.46751233355683	39.0	35.0	40.0	30.0	40.0
29	36.43411192072064	39.0	35.0	40.0	30.0	40.0
30	36.406073358965465	39.0	35.0	40.0	30.0	40.0
31	36.405365927113074	39.0	35.0	40.0	30.0	40.0
32	36.28398142539731	38.0	35.0	40.0	30.0	40.0
33	36.12847670850265	38.0	35.0	40.0	30.0	40.0
34	36.00522719962892	38.0	35.0	40.0	30.0	40.0
35	35.84100653784863	38.0	35.0	40.0	30.0	40.0
36	35.67245807338246	38.0	34.0	40.0	29.0	40.0
37	35.55615188043022	38.0	34.0	40.0	29.0	40.0
38	35.24953005486268	38.0	34.0	40.0	27.0	40.0
39	35.12769131586241	38.0	34.0	40.0	27.0	40.0
40	35.009098718114615	37.0	34.0	40.0	27.0	40.0
41	34.91112131997937	37.0	34.0	40.0	27.0	40.0
42	34.80747743631467	37.0	34.0	40.0	27.0	40.0
43	34.59862306653241	36.0	33.0	40.0	26.0	40.0
44	34.43134395676629	36.0	33.0	40.0	26.0	40.0
45	34.20429900347246	36.0	33.0	40.0	25.0	40.0
46	34.018153383044314	35.0	33.0	39.0	24.0	40.0
47	33.89391734981735	35.0	33.0	39.0	24.0	40.0
48	33.697073569174805	35.0	33.0	39.0	23.0	40.0
49	33.56405163994433	35.0	33.0	39.0	24.0	40.0
50	33.36493160008821	35.0	32.0	39.0	23.0	40.0
51	33.18582283072773	35.0	32.0	38.0	23.0	40.0
52	33.00224083867297	35.0	32.0	38.0	23.0	40.0
53	32.79338406598971	35.0	32.0	38.0	23.0	40.0
54	32.62495761549633	35.0	31.0	37.0	22.0	40.0
55	32.47353364746666	35.0	31.0	37.0	22.0	40.0
56	32.25956172153795	35.0	31.0	37.0	21.0	40.0
57	32.10821544730714	35.0	31.0	36.0	21.0	39.0
58	31.907013605225437	35.0	31.0	36.0	20.0	39.0
59	31.386481439191744	34.0	30.0	35.0	20.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	12.0
13	13.0
14	70.0
15	260.0
16	1126.0
17	2988.0
18	6823.0
19	12887.0
20	21298.0
21	32842.0
22	47630.0
23	65602.0
24	88014.0
25	113905.0
26	145203.0
27	181349.0
28	220344.0
29	264264.0
30	314864.0
31	380884.0
32	470056.0
33	602281.0
34	901888.0
35	1310264.0
36	1269159.0
37	1908405.0
38	2385974.0
39	488008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.94092227612038	19.77295611466216	33.80286315443231	17.483258454785148
2	22.753585706761708	21.580934422339933	35.6143479176371	20.05113195326126
3	22.230883543136258	21.207870693311694	35.855697073569175	20.705548689982876
4	21.40118852823026	23.725701553644587	31.561979983300724	23.311129934824425
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	100.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.54569151841805	0.39326589665852585	0.03906939026298239	0.0219731946604336
12	99.27301565320906	0.6365907711870455	0.051929360406492535	0.038464215197405435
13	98.55313321380532	0.8337176491654191	0.20885659469058554	0.4042925423386705
14	66.55341586615948	20.03216683466208	6.460546412566709	6.953870886611729
15	40.238864378309884	25.569233896424997	14.810333684631527	19.38156804063359
16	34.92530270767784	15.87082289035527	23.81567684648257	25.38819755548432
17	25.907918341948477	15.556220852588432	26.384502605723636	32.151358199739455
18	23.655376651171217	18.11496332728099	31.297384985143843	26.93227503640395
19	28.66370759791955	16.90994407648416	28.501298189512	25.925050136084295
20	31.70277218238054	17.37166595261942	25.084248378798296	25.841313486201738
21	32.92025981945492	18.041327761213584	22.679131374429108	26.35928104490239
22	32.26752511343028	19.68891687724682	22.299312823713038	25.74424518560986
23	30.407261460013324	22.05426142032096	20.607465831192822	26.931011288472895
24	30.385911239685104	24.94250391895353	19.612647019717492	25.058937821643873
25	30.14661789904932	21.84578750986302	23.035170104921338	24.972424486166318
26	28.978683420285716	20.830908924890476	23.149138809182784	27.04126884564102
27	31.41924766415774	21.051646530059667	21.60553300882897	25.92357279695362
28	33.07289743048007	20.203422478587036	20.038138488617992	26.6855416023149
29	32.093955564842815	19.17540803483815	18.656114430773226	30.07452196954581
30	29.11980490579827	18.25183078806787	21.453206884969926	31.175157421163934
31	26.921862465422702	18.527799517604276	21.685095730685706	32.86524228628732
32	28.420685310923822	23.933054110304546	18.678630503066014	28.96763007570562
33	32.22522515627311	22.829410582625176	18.708791360378367	26.236572900723342
34	31.11930332246451	20.775001428391146	21.78229752577734	26.323397723367002
35	33.05844442597276	21.426232096385164	20.59087691468936	24.924446562952713
36	36.22011925864631	20.14810235788665	19.85865958350784	23.773118799959203
37	35.59144916111167	20.013201716062895	19.806080549869183	24.589268572956257
38	30.756803458183917	23.48752956680679	23.406480606186065	22.349186368823233
39	29.690840977970023	26.050695159258048	23.57014486289136	20.68831899988057
40	30.535478477215783	28.585132735361036	18.87696773117554	22.00242105624764
41	31.333366202645717	26.698196880892112	18.13599316080979	23.83244375565238
42	32.29358323978335	25.870878068081836	17.294061150092492	24.541477542042312
43	32.10445713215724	24.474525600596202	17.32737247759918	26.093644789647374
44	31.111925526444463	23.29445202198779	18.831294812991114	26.762327638576632
45	27.670241530708452	24.036352154218545	20.267304046538676	28.02610226853433
46	27.638549936465516	23.369173343319908	19.7022218290562	29.290054891158373
47	28.287635792830063	19.466016661181527	22.098608293141105	30.147739252847305
48	29.917514638561858	18.801276349811836	20.56923300646167	30.711976005164637
49	30.662930125952954	20.370023614287046	20.881289749099757	28.085756510660246
50	28.961765217349516	23.129755407817687	19.921393098761545	27.987086276071256
51	27.486739991338876	22.598678689240646	22.07204288768894	27.842538431731533
52	28.7908922576572	21.712070519270465	23.023467087109076	26.47357013596326
53	29.97447229179263	21.547098016467526	21.97501908526365	26.50341060647619
54	28.42246523758728	20.850185530655764	22.234381099029957	28.492968132726997
55	26.512719622926095	21.917536353667156	23.092296851185136	28.47744717222161
56	26.230325358134593	21.318350741312756	24.107656016356817	28.343667884195835
57	26.849143561586974	20.258057327521993	25.75688266492044	27.135916445970594
58	26.892547073275487	20.736038833727974	24.636899410470487	27.73451468252605
59	26.95413253583126	20.641142043665518	22.50926407330165	29.895461347201575
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	27.5
4	91.0
5	128.0
6	217.5
7	307.0
8	568.0
9	1216.0
10	1603.0
11	2012.5
12	2422.0
13	3046.0
14	3881.0
15	4092.0
16	4978.5
17	5865.0
18	6955.5
19	12136.5
20	16227.0
21	22400.5
22	28574.0
23	35521.0
24	42468.0
25	47713.0
26	57173.5
27	61389.0
28	65736.5
29	70084.0
30	73964.0
31	80035.5
32	82227.0
33	91960.0
34	101693.0
35	108256.5
36	122739.5
37	130659.0
38	150244.0
39	169829.0
40	200350.5
41	230872.0
42	242751.0
43	282288.5
44	309947.0
45	329567.0
46	349187.0
47	376453.0
48	439206.5
49	474694.0
50	517735.0
51	560776.0
52	616286.0
53	772208.5
54	872621.0
55	956582.0
56	1040543.0
57	1037287.0
58	978033.0
59	922035.0
60	822913.0
61	723791.0
62	666208.5
63	608626.0
64	567288.0
65	465403.0
66	404856.0
67	357159.5
68	309463.0
69	271472.5
70	191931.5
71	150381.0
72	122334.5
73	94288.0
74	68133.0
75	33325.0
76	24672.0
77	17929.5
78	11187.0
79	7302.0
80	3417.0
81	2866.0
82	1423.5
83	532.0
84	453.5
85	375.0
86	269.5
87	218.0
88	272.0
89	150.5
90	29.0
91	14.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1.1236418E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.714320359350879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2939400106809	9.288746158402665
2	7.680222643195436	1.7993717694706381
3	3.198044733950498	1.1238876161109352
4	1.9169533579931075	0.8982322299785875
5	1.158636185700424	0.6786317729615561
6	0.776081765979213	0.5454762259038767
7	0.5032439886121366	0.41266129110640654
8	0.35274894105594135	0.33057712815608614
9	0.27080952681846626	0.28551145981641635
>10	1.7336995327405396	4.32345297798018
>50	0.6873686612557969	6.035870657942402
>100	2.2372172838013746	58.58825556995356
>500	0.17410244695083932	13.173885539587518
>1k	0.016930921265403426	2.5154396026294714
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.019285505398606566	0.0	0.0	0.0	0.0
11	0.020771744162597014	0.0	0.0	0.0	0.0
12	0.027989346782933848	0.0	0.0	0.0	0.0
13	0.03366731283937639	0.0	8.89963331730806E-6	0.0	0.0
14	0.0518937618732233	0.0	8.89963331730806E-6	0.0	0.0
15	0.07465902389889732	0.0	8.89963331730806E-6	0.0	0.0
16	0.08555217507928238	0.0	8.89963331730806E-6	0.0	0.0
17	0.09445180839659044	0.0	8.89963331730806E-6	0.0	0.0
18	0.10967908100250454	0.0	8.89963331730806E-6	0.0	0.0
19	0.1218893779138512	0.0	8.89963331730806E-6	0.0	0.0
20	0.13353009829289014	0.0	8.89963331730806E-6	0.0	0.0
21	0.14794750426692918	0.0	8.89963331730806E-6	0.0	0.0
22	0.16248060547409326	0.0	8.89963331730806E-6	0.0	0.0
23	0.17858004214510353	0.0	8.89963331730806E-6	0.0	0.0
24	0.195667338114335	0.0	8.89963331730806E-6	0.0	0.0
25	0.21246094618409533	0.0	8.89963331730806E-6	0.0	0.0
26	0.23145276368323073	0.0	8.89963331730806E-6	0.0	0.0
27	0.2530877722776066	0.0	8.89963331730806E-6	0.0	0.0
28	0.2736548248739055	0.0	8.89963331730806E-6	0.0	0.0
29	0.29431977343669485	0.0	8.89963331730806E-6	0.0	0.0
30	0.3197994236241478	0.0	8.89963331730806E-6	0.0	0.0
31	0.34501208481208157	0.0	8.89963331730806E-6	0.0	0.0
32	0.3739002945600635	0.0	8.89963331730806E-6	0.0	0.0
33	0.397110538251603	0.0	8.89963331730806E-6	0.0	0.0
34	0.4165740363165557	0.0	8.89963331730806E-6	0.0	0.0
35	0.4347114890172295	0.0	8.89963331730806E-6	0.0	0.0
36	0.4523060640855475	0.0	8.89963331730806E-6	0.0	0.0
37	0.4705325131193945	0.0	8.89963331730806E-6	0.0	0.0
38	0.4929595890790108	0.0	8.89963331730806E-6	0.0	0.0
39	0.5213494193612235	0.0	8.89963331730806E-6	0.0	0.0
40	0.5625280227204079	0.0	8.89963331730806E-6	0.0	0.0
41	0.5972988900911305	0.0	8.89963331730806E-6	0.0	0.0
42	0.624816556308247	0.0	8.89963331730806E-6	0.0	0.0
43	0.648738770665171	0.0	8.89963331730806E-6	0.0	0.0
44	0.6730258699881048	0.0	8.89963331730806E-6	0.0	0.0
45	0.6971349766446923	0.0	8.89963331730806E-6	0.0	0.0
46	0.7222764407660875	0.0	8.89963331730806E-6	0.0	0.0
47	0.7444721262594538	0.0	8.89963331730806E-6	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACTA	30	7.016042E-7	53.0	21
TCGACAA	14245	0.0	50.65602	2
GACAATA	253980	0.0	50.25484	4
TAGGGGC	165430	0.0	50.207912	9
TGACAAT	86340	0.0	50.16707	3
CAACAAT	70955	0.0	49.96364	3
TTACAAT	148675	0.0	49.898605	3
ATAGGGG	776580	0.0	49.881413	8
GAACAAT	49390	0.0	49.861206	3
TAGGGAT	49555	0.0	49.860962	9
AACAATA	281950	0.0	49.821316	4
AATAGGG	1166065	0.0	49.806316	7
CAATAGG	1182185	0.0	49.798534	6
ACAATAG	1190560	0.0	49.792114	5
TTAACAA	37750	0.0	49.79192	2
CGAACAA	10230	0.0	49.787876	2
TAACAAT	95670	0.0	49.689926	3
CACAATA	280165	0.0	49.62702	4
TAGGGGA	183020	0.0	49.59737	9
ATAGGGC	80070	0.0	49.554703	8
>>END_MODULE