FastQCFastQC Report
Fri 17 Jun 2016
SRR1528023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372672
Sequences flagged as poor quality0
Sequence length52
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGTAATGGGGTGGTGGCTCATGCCTCTAATCCCAGCACTTTGGGAGGCT13400.35956551605701526No Hit
GAGAGTAATGGGGCACTTTGGGAGGCTGAGGTGGGCAGATCACAAGGTCAGG9740.26135583032800963No Hit
TCTCGTAATGGGGGCAGCCACAGGTCCCAGAGCTCCCAAGTCACACTCCCTT9180.2463292117465224No Hit
ACTGGTAATGGGGGCCCCCACTGGCTGCTCCGAAAAGCCATCTTTGCATTGT9030.24230422462648118No Hit
CATTGTAATGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGA8380.2248626137729693No Hit
ACTAGTAATGGGGGGCGCGGTGGCTCAGGACTGTAATCCCAGCACTTTGGGA7380.19802936630602783No Hit
GTTGGTAATGGGGCTCAATGGCCTGGAAATCGCCATCCTGGCCGAGAACCTC7340.19695603640735018No Hit
AAGGGTAATGGGGATGAAGACTGCCGGGCAGAGGTGGTGCACACCTTTAATC7070.18971105959127596No Hit
TGTGGTAATGGGGATTTAGGTTGCTGAGTATTTTTTATTTGCCCATCCATTC7060.18944272711660656No Hit
GCGAGTAATGGGGCATATTTTGCTTTTTCTTGAAGAAGAGTATTTGATCAGT6350.17039112141507815No Hit
GGGCGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGCGGGCTAACGG5810.15590116778292976No Hit
TTTTGTAATGGGTACCCTTCCAATATGACCAGTTTCTTATAGGAAAAGAAAA5580.14972952086553323No Hit
TTTGGTAATGGGGCGTCCTTAAAAATACAGTAAAAAAGGCTGGGCACAGTGG5500.14758286106817792No Hit
CGCAGTAATGGGGAGGAATAAATAACACATGAAAAATAACCAGAAAGATGGT5450.14624119869483085No Hit
CAAGGTAATGGGGACCTCACGCAAGATGGCGGAGCTGGAGGAGGTGACTCTG5200.13953288682809548No Hit
CATTGTAATGGGGGCTTTTTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCG5160.1384595569294178No Hit
GTCTGTAATGGGGCATCCTTACCTGGGAAACAGAGCAGCAGGAGCCCCAGGA5140.13792289198007898No Hit
TAACGTAATGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGG5130.13765455950540958No Hit
ACAGGTAATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAA5110.13711789455607074No Hit
CTTTGTAATGGGGTAAGACGGGCAATTACACTATAGAAGTCTGCATTCACAG4890.13121458011334364No Hit
GCGGGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG4840.12987291773999657No Hit
TCTTGTAATGGGGGTTGAGGTAATGGGGTGACCAACATGATGAAACCCCGTT4840.12987291773999657No Hit
CCGGGTAATGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGT4740.1271895929933024No Hit
CGTCGTAATGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC4740.1271895929933024No Hit
GTTCGTAATGGGGGACTTTGGATCCAGCTCTTCTCAGCTCTGCAGCATCAGA4680.12557959814528594No Hit
TCTTGTAATGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGT4630.12423793577193887No Hit
ATGAGTAATGGGGGCATTATTTACAGTAGCCAAAAACTGGAAATAACTCAAA4590.1231646058732612No Hit
TTTTGTAATGGGGAGCAGAATACCCACCTTGTTTTCTTGCAAAGGGCAAGAT4570.12262794092392237No Hit
TGTGGTAATGGGGGCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAGAC4460.11967628370255882No Hit
TGGGGTAATGGGGGCTTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCC4390.11779795637987293No Hit
CGGAGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGGTTAGTCGCGG4290.11511463163317878No Hit
AAGGGTAATGGGGTGTCGTCCCCACCTCTGTGTGATGCGCAAAGTACACCTG4220.11323630431049286No Hit
TGGTGTAATGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGA4210.11296797183582347No Hit
GGCCGTAATGGGGGCTCTAGCTGCCATCTTGCGTCCCCGCGTGTGTGCGCCT4130.11082131203846814No Hit
GCATGTAATGGGGGCCTTTCCTACTTGGGCCCGGCAGAATGGCTCCCGCAAA4110.11028464708912931No Hit
AGTCGTAATGGGGGCTTCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCA4070.10921131719045164No Hit
TCTAGTAATGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTC4050.10867465224111282No Hit
TTGGGTAATGGGTATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCAT4030.108137987291774No Hit
GCTTGTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAT4030.108137987291774No Hit
AGCTGTAATGGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATG4010.10760132234243518No Hit
CTGGGTAATGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGA3950.10599132749441868No Hit
GCGCGTAATGGGGCATCAGAAGGGAGTGATGTGTGTGTGTGTGTGTGTGTAC3820.10250300532371631No Hit
TTAGGTAATGGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATG3750.1006246780010304No Hit
GGCCGTAATGGGGATTGAAACAATGCATATTTTATTTAATAGCTCCCGTTCT3740.10035634552636098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAG405.5933924E-946.032
TCAAAAC301.857994E-646.015
TGATCCT750.046.022
CCCTGAA453.092282E-1046.038
TATTTTG850.046.016
CTTGTGA206.305938E-446.030
CTGCTTA405.5933924E-946.035
CCTTCCG1000.046.045
CTTGTCT750.046.043
ATCCTTA600.046.015
ATTTAGG501.6370905E-1146.014
GTGTTCC551.8189894E-1246.024
TTGATCA850.046.044
ATCCTGA206.305938E-446.026
CTTAAAT206.305938E-446.042
CTAGATA253.4126813E-546.045
GTGTTAC206.305938E-446.038
CTTCGGA800.046.016
ACAACAT301.857994E-646.022
ATCCTAT206.305938E-446.019
Produced by FastQC (version 0.11.5)