##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528023_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	372672
Sequences flagged as poor quality	0
Sequence length	52
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.534443156448567	31.0	31.0	33.0	27.0	34.0
2	31.612780139103556	31.0	31.0	34.0	28.0	34.0
3	31.972383221707023	33.0	31.0	34.0	30.0	34.0
4	35.675811437403404	37.0	35.0	37.0	33.0	37.0
5	35.85222393955006	37.0	35.0	37.0	35.0	37.0
6	33.57066267387945	35.0	33.0	35.0	29.0	37.0
7	34.86333558732612	35.0	35.0	37.0	32.0	37.0
8	35.6614449167096	37.0	35.0	37.0	33.0	37.0
9	37.89795047655848	39.0	37.0	39.0	35.0	39.0
10	37.58557390949682	39.0	37.0	39.0	35.0	39.0
11	38.135134917568266	39.0	39.0	39.0	37.0	39.0
12	38.19520382534776	39.0	39.0	39.0	37.0	39.0
13	37.932074317362186	39.0	38.0	39.0	35.0	39.0
14	38.1209830628542	40.0	38.0	40.0	34.0	40.0
15	37.4348220419028	39.0	36.0	40.0	33.0	40.0
16	37.57250075133093	39.0	37.0	40.0	33.0	40.0
17	37.579490812296065	40.0	37.0	40.0	33.0	40.0
18	37.619402584578395	40.0	37.0	40.0	33.0	40.0
19	37.713598016486344	40.0	37.0	40.0	33.0	40.0
20	37.660433303280094	40.0	37.0	40.0	33.0	40.0
21	37.60254593851966	40.0	37.0	40.0	33.0	40.0
22	37.659164090674906	40.0	37.0	40.0	33.0	40.0
23	37.52388159024558	40.0	37.0	40.0	33.0	40.0
24	37.66020253735188	40.0	37.0	40.0	33.0	40.0
25	37.62643826206423	40.0	37.0	40.0	33.0	40.0
26	37.52479123733471	40.0	37.0	40.0	33.0	40.0
27	37.30644373604671	39.0	37.0	40.0	33.0	40.0
28	36.90328224283016	39.0	36.0	40.0	32.0	40.0
29	36.59969356431393	39.0	35.0	40.0	31.0	40.0
30	36.3984576249356	39.0	35.0	40.0	31.0	40.0
31	36.239247917739995	39.0	35.0	40.0	30.0	40.0
32	36.05018622273742	38.0	35.0	40.0	30.0	40.0
33	35.813170831186675	38.0	35.0	40.0	30.0	40.0
34	35.51309999141336	38.0	35.0	40.0	28.0	40.0
35	35.284625622531344	38.0	35.0	40.0	26.0	40.0
36	34.89583601665808	38.0	35.0	40.0	25.0	40.0
37	34.695093809033146	38.0	35.0	40.0	23.0	40.0
38	34.33941911385884	38.0	34.0	40.0	21.0	40.0
39	33.82931907951228	37.0	33.0	40.0	18.0	40.0
40	33.21349873347072	37.0	33.0	40.0	15.0	40.0
41	32.48038757942641	36.0	32.0	40.0	10.0	40.0
42	31.777699961360124	35.0	31.0	40.0	10.0	40.0
43	31.356366992958957	35.0	30.0	40.0	9.0	40.0
44	30.97572932766615	35.0	28.0	40.0	8.0	40.0
45	30.623371221878756	35.0	26.0	40.0	8.0	40.0
46	30.282382363043105	35.0	24.0	39.0	8.0	40.0
47	29.940078675081573	35.0	23.0	39.0	8.0	40.0
48	29.577427335565858	35.0	23.0	39.0	8.0	40.0
49	29.21691460587326	35.0	20.0	39.0	8.0	40.0
50	28.808617229091535	35.0	20.0	38.0	8.0	40.0
51	28.426914283874293	35.0	18.0	38.0	8.0	40.0
52	27.749777284046026	34.0	15.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	5.0
16	13.0
17	33.0
18	138.0
19	321.0
20	656.0
21	1147.0
22	1904.0
23	2827.0
24	4093.0
25	5676.0
26	7648.0
27	9571.0
28	12341.0
29	15367.0
30	17468.0
31	16188.0
32	14561.0
33	15473.0
34	20196.0
35	28973.0
36	34133.0
37	59054.0
38	83395.0
39	21491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.13391937145801	20.54648591791173	36.76101236476043	6.5585823458698265
2	30.023720590760778	18.30590975442212	34.487431306886485	17.18293834793062
3	22.853608535119356	21.54173106646059	36.39286020951399	19.21180018890606
4	26.165636269963937	21.6925339172248	32.28925167439464	19.852578138416625
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	98.56522625794264	0.9633135840631977	0.29919070925639707	0.17226944873776404
12	97.69368238021639	1.417332131203847	0.3968637300360639	0.49212175854370604
13	87.00921990382963	8.652648978189937	2.4952236819508844	1.842907436029538
14	29.42238751502662	47.2101472608621	11.516293147861926	11.851172076249355
15	22.54448952430019	48.81397046196119	16.566578653614975	12.074961360123648
16	31.50276919113859	35.4295466254508	19.3422097715954	13.725474411815217
17	15.311051004636786	39.185664605873264	20.995942812983	24.507341576506956
18	17.043137128627855	45.99164949338829	22.843143568607246	14.122069809376612
19	22.21927056500086	40.30783101494075	26.101773140992616	11.371125279065772
20	18.349647947793237	40.28529108706852	27.687349733814187	13.67771123132406
21	26.311877468658768	37.46672677314099	17.08177700498025	19.139618753219988
22	23.945453374549203	43.01262235960845	18.968959299330244	14.072964966512108
23	18.33301133436373	42.96754250386399	23.779087240254164	14.920358921518117
24	18.36413790142538	49.08686458869999	17.50842563970462	15.040571870170016
25	18.95151768847673	41.61085351193543	23.43240168298128	16.00522711660656
26	17.80546968916366	45.370459814528594	15.626073329898677	21.19799716640907
27	19.596857290056672	50.3324639361154	14.650416452000686	15.420262321827236
28	20.598810750472264	46.68072728833935	15.033863558303281	17.68659840288511
29	24.209760862098577	42.55296883049974	13.514833419199727	19.72243688820196
30	27.475367078825347	43.69257685042074	13.298289112141507	15.5337669586124
31	20.70104542332131	46.895661600549545	13.784239223767816	18.619053752361324
32	21.760958698265497	52.003370255881855	14.108921518117809	12.126749527734844
33	23.026414648806455	50.59247810407007	13.38415550403572	12.996951743087756
34	18.3429396359265	54.298954576678696	15.118924952773485	12.23918083462133
35	21.62732912588013	50.31072900566718	15.939485660312553	12.122456208140134
36	18.116198694830842	48.95699167095999	19.990501030396704	12.936308603812469
37	18.68103855400996	50.355808861411646	17.867722823286964	13.09542976129143
38	20.310353340202646	49.727910870685214	17.629175253305856	12.332560535806286
39	20.53199596427958	47.68080242143225	17.307712948651897	14.47948866563627
40	25.1596578224283	45.51213936115405	16.708794865189766	12.61940795122789
41	26.084063197664438	44.795423321312036	16.333397733127253	12.787115747896275
42	21.007749441868455	45.978769534604155	18.50715911042418	14.506321913103212
43	22.101472608620988	49.68336767989009	14.806049287308948	13.409110424179977
44	22.560589472780354	45.37072814700326	19.157597029022842	12.911085351193544
45	25.22700927357032	41.66827666151468	19.379776318049117	13.724937746865876
46	30.916462733985917	40.32178430362356	14.705961274257257	14.055791688133265
47	30.198136699295898	40.43663060278207	14.386645629400652	14.978587068521382
48	27.553183496479477	42.59939034861755	14.173589644513138	15.673836510389833
49	25.209567662716815	44.14122874806801	13.792557530482568	16.856646058732615
50	22.402809977674735	47.47123475871544	14.522421861583377	15.603533402026448
51	21.546829383479306	47.49431135153701	15.85791258801305	15.100946676970633
52	21.745127082260005	46.77357032457496	16.081701871887343	15.399600721277693
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	15.5
6	31.0
7	111.0
8	191.0
9	613.5
10	1036.0
11	1680.5
12	2325.0
13	2715.0
14	2835.0
15	2565.0
16	2727.0
17	2889.0
18	3372.0
19	3855.0
20	4978.0
21	6101.0
22	8052.0
23	10003.0
24	12311.5
25	14620.0
26	16727.5
27	18835.0
28	19472.0
29	20109.0
30	19287.5
31	18466.0
32	17769.0
33	17072.0
34	15805.0
35	14538.0
36	14923.0
37	15308.0
38	15190.0
39	16283.5
40	17495.0
41	18495.0
42	19495.0
43	20963.5
44	22432.0
45	22971.5
46	23511.0
47	23258.0
48	23005.0
49	20642.5
50	18280.0
51	17180.5
52	16081.0
53	14807.5
54	13534.0
55	12391.0
56	11248.0
57	11763.0
58	12278.0
59	11112.0
60	9946.0
61	8261.5
62	6577.0
63	5583.5
64	4058.5
65	3527.0
66	3033.0
67	2539.0
68	1591.0
69	643.0
70	633.0
71	623.0
72	571.5
73	520.0
74	295.5
75	71.0
76	66.0
77	61.0
78	76.5
79	92.0
80	46.0
81	0.0
82	0.0
83	0.0
84	0.5
85	1.0
86	0.5
87	0.0
88	1.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	372672.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.46159768496724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.93727705246516	38.15922720130804
2	5.88091695263507	5.347117610387981
3	2.494256195463753	3.401786150442305
4	1.5910920321911104	2.893343433889168
5	1.0611593237589299	2.412099912819018
6	0.8551837094556369	2.3326810647606044
7	0.6164361914151105	1.9616921892797048
8	0.49605391142469046	1.8041122680994919
9	0.3928137165985719	1.607214523422691
>10	2.4139949603590236	18.58109045202779
>50	0.1043263816931734	3.3323868642501226
>100	0.14522706542515618	14.582897877963399
>500	0.01066974358225637	3.223247556469005
>1k	5.927635323475761E-4	0.3611028948807346
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCGGTAATGGGGTGGTGGCTCATGCCTCTAATCCCAGCACTTTGGGAGGCT	1340	0.35956551605701526	No Hit
GAGAGTAATGGGGCACTTTGGGAGGCTGAGGTGGGCAGATCACAAGGTCAGG	974	0.26135583032800963	No Hit
TCTCGTAATGGGGGCAGCCACAGGTCCCAGAGCTCCCAAGTCACACTCCCTT	918	0.2463292117465224	No Hit
ACTGGTAATGGGGGCCCCCACTGGCTGCTCCGAAAAGCCATCTTTGCATTGT	903	0.24230422462648118	No Hit
CATTGTAATGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGA	838	0.2248626137729693	No Hit
ACTAGTAATGGGGGGCGCGGTGGCTCAGGACTGTAATCCCAGCACTTTGGGA	738	0.19802936630602783	No Hit
GTTGGTAATGGGGCTCAATGGCCTGGAAATCGCCATCCTGGCCGAGAACCTC	734	0.19695603640735018	No Hit
AAGGGTAATGGGGATGAAGACTGCCGGGCAGAGGTGGTGCACACCTTTAATC	707	0.18971105959127596	No Hit
TGTGGTAATGGGGATTTAGGTTGCTGAGTATTTTTTATTTGCCCATCCATTC	706	0.18944272711660656	No Hit
GCGAGTAATGGGGCATATTTTGCTTTTTCTTGAAGAAGAGTATTTGATCAGT	635	0.17039112141507815	No Hit
GGGCGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGCGGGCTAACGG	581	0.15590116778292976	No Hit
TTTTGTAATGGGTACCCTTCCAATATGACCAGTTTCTTATAGGAAAAGAAAA	558	0.14972952086553323	No Hit
TTTGGTAATGGGGCGTCCTTAAAAATACAGTAAAAAAGGCTGGGCACAGTGG	550	0.14758286106817792	No Hit
CGCAGTAATGGGGAGGAATAAATAACACATGAAAAATAACCAGAAAGATGGT	545	0.14624119869483085	No Hit
CAAGGTAATGGGGACCTCACGCAAGATGGCGGAGCTGGAGGAGGTGACTCTG	520	0.13953288682809548	No Hit
CATTGTAATGGGGGCTTTTTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCG	516	0.1384595569294178	No Hit
GTCTGTAATGGGGCATCCTTACCTGGGAAACAGAGCAGCAGGAGCCCCAGGA	514	0.13792289198007898	No Hit
TAACGTAATGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGG	513	0.13765455950540958	No Hit
ACAGGTAATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAA	511	0.13711789455607074	No Hit
CTTTGTAATGGGGTAAGACGGGCAATTACACTATAGAAGTCTGCATTCACAG	489	0.13121458011334364	No Hit
GCGGGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG	484	0.12987291773999657	No Hit
TCTTGTAATGGGGGTTGAGGTAATGGGGTGACCAACATGATGAAACCCCGTT	484	0.12987291773999657	No Hit
CCGGGTAATGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGT	474	0.1271895929933024	No Hit
CGTCGTAATGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	474	0.1271895929933024	No Hit
GTTCGTAATGGGGGACTTTGGATCCAGCTCTTCTCAGCTCTGCAGCATCAGA	468	0.12557959814528594	No Hit
TCTTGTAATGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGT	463	0.12423793577193887	No Hit
ATGAGTAATGGGGGCATTATTTACAGTAGCCAAAAACTGGAAATAACTCAAA	459	0.1231646058732612	No Hit
TTTTGTAATGGGGAGCAGAATACCCACCTTGTTTTCTTGCAAAGGGCAAGAT	457	0.12262794092392237	No Hit
TGTGGTAATGGGGGCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAGAC	446	0.11967628370255882	No Hit
TGGGGTAATGGGGGCTTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCC	439	0.11779795637987293	No Hit
CGGAGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGGTTAGTCGCGG	429	0.11511463163317878	No Hit
AAGGGTAATGGGGTGTCGTCCCCACCTCTGTGTGATGCGCAAAGTACACCTG	422	0.11323630431049286	No Hit
TGGTGTAATGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGA	421	0.11296797183582347	No Hit
GGCCGTAATGGGGGCTCTAGCTGCCATCTTGCGTCCCCGCGTGTGTGCGCCT	413	0.11082131203846814	No Hit
GCATGTAATGGGGGCCTTTCCTACTTGGGCCCGGCAGAATGGCTCCCGCAAA	411	0.11028464708912931	No Hit
AGTCGTAATGGGGGCTTCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCA	407	0.10921131719045164	No Hit
TCTAGTAATGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTC	405	0.10867465224111282	No Hit
TTGGGTAATGGGTATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCAT	403	0.108137987291774	No Hit
GCTTGTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAT	403	0.108137987291774	No Hit
AGCTGTAATGGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATG	401	0.10760132234243518	No Hit
CTGGGTAATGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGA	395	0.10599132749441868	No Hit
GCGCGTAATGGGGCATCAGAAGGGAGTGATGTGTGTGTGTGTGTGTGTGTAC	382	0.10250300532371631	No Hit
TTAGGTAATGGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATG	375	0.1006246780010304	No Hit
GGCCGTAATGGGGATTGAAACAATGCATATTTTATTTAATAGCTCCCGTTCT	374	0.10035634552636098	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0711081057873948	0.0	0.0	0.0	0.0
12	0.14060621672677315	0.0	0.0	0.0	0.0
13	0.29811737935771937	0.0	0.0	0.0	0.0
14	0.7100077279752705	0.0	0.0	0.0	0.0
15	1.0564249527734844	0.0	0.0	0.0	0.0
16	1.2531126567061652	0.0	0.0	0.0	0.0
17	1.3569573244032285	0.0	0.0	0.0	0.0
18	1.5018568607247123	0.0	0.0	0.0	0.0
19	1.6397797527047913	0.0	0.0	0.0	0.0
20	1.8042675596771425	0.0	0.0	0.0	0.0
21	1.9682187017001544	0.0	0.0	0.0	0.0
22	2.147733127253993	0.0	0.0	0.0	0.0
23	2.3508608105787396	0.0	0.0	0.0	0.0
24	2.5800167439464192	0.0	0.0	0.0	0.0
25	2.827955950540958	0.0	0.0	0.0	0.0
26	3.090653443242315	0.0	0.0	0.0	0.0
27	3.375354198866564	0.0	0.0	0.0	0.0
28	3.6603232869654816	0.0	0.0	0.0	0.0
29	3.95441567920316	0.0	0.0	0.0	0.0
30	4.2935879271853	0.0	0.0	0.0	0.0
31	4.579093680233557	0.0	0.0	0.0	0.0
32	4.806371286278551	0.0	0.0	0.0	0.0
33	4.996350678344496	0.0	0.0	0.0	0.0
34	5.17291344667697	0.0	0.0	0.0	0.0
35	5.329619611883909	0.0	0.0	0.0	0.0
36	5.475324145629401	0.0	0.0	0.0	0.0
37	5.618077022153529	0.0	0.0	0.0	0.0
38	5.7833698265498885	0.0	0.0	0.0	0.0
39	5.9612742572557105	0.0	0.0	0.0	0.0
40	6.138105358062854	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAG	40	5.5933924E-9	46.0	32
TCAAAAC	30	1.857994E-6	46.0	15
TGATCCT	75	0.0	46.0	22
CCCTGAA	45	3.092282E-10	46.0	38
TATTTTG	85	0.0	46.0	16
CTTGTGA	20	6.305938E-4	46.0	30
CTGCTTA	40	5.5933924E-9	46.0	35
CCTTCCG	100	0.0	46.0	45
CTTGTCT	75	0.0	46.0	43
ATCCTTA	60	0.0	46.0	15
ATTTAGG	50	1.6370905E-11	46.0	14
GTGTTCC	55	1.8189894E-12	46.0	24
TTGATCA	85	0.0	46.0	44
ATCCTGA	20	6.305938E-4	46.0	26
CTTAAAT	20	6.305938E-4	46.0	42
CTAGATA	25	3.4126813E-5	46.0	45
GTGTTAC	20	6.305938E-4	46.0	38
CTTCGGA	80	0.0	46.0	16
ACAACAT	30	1.857994E-6	46.0	22
ATCCTAT	20	6.305938E-4	46.0	19
>>END_MODULE