FastQCFastQC Report
Fri 17 Jun 2016
SRR1528022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371588
Sequences flagged as poor quality0
Sequence length52
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGTAATGGGGTGGTGGCTCATGCCTCTAATCCCAGCACTTTGGGAGGCT12540.3374705318793933No Hit
GAGAGTAATGGGGCACTTTGGGAGGCTGAGGTGGGCAGATCACAAGGTCAGG9250.24893161243097192No Hit
TCTCGTAATGGGGGCAGCCACAGGTCCCAGAGCTCCCAAGTCACACTCCCTT9120.24543311409410423No Hit
ACTGGTAATGGGGGCCCCCACTGGCTGCTCCGAAAAGCCATCTTTGCATTGT8380.22551858509962647No Hit
ACTAGTAATGGGGGGCGCGGTGGCTCAGGACTGTAATCCCAGCACTTTGGGA7750.20856432392865215No Hit
AAGGGTAATGGGGATGAAGACTGCCGGGCAGAGGTGGTGCACACCTTTAATC7190.19349386955445277No Hit
CATTGTAATGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGA6980.187842449164128No Hit
GTTGGTAATGGGGCTCAATGGCCTGGAAATCGCCATCCTGGCCGAGAACCTC6660.1792307609502998No Hit
TGTGGTAATGGGGATTTAGGTTGCTGAGTATTTTTTATTTGCCCATCCATTC6520.17546314735674995No Hit
GCGAGTAATGGGGCATATTTTGCTTTTTCTTGAAGAAGAGTATTTGATCAGT5790.15581773361895432No Hit
TTTTGTAATGGGTACCCTTCCAATATGACCAGTTTCTTATAGGAAAAGAAAA5180.13940170296134427No Hit
GGGCGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGCGGGCTAACGG5150.1385943571912979No Hit
CATTGTAATGGGGGCTTTTTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCG5120.1377870114212515No Hit
CGCAGTAATGGGGAGGAATAAATAACACATGAAAAATAACCAGAAAGATGGT5070.13644143513784082No Hit
ACAGGTAATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAA5010.13482674359774804No Hit
CAAGGTAATGGGGACCTCACGCAAGATGGCGGAGCTGGAGGAGGTGACTCTG4920.13240470628760886No Hit
GTCTGTAATGGGGCATCCTTACCTGGGAAACAGAGCAGCAGGAGCCCCAGGA4800.12917532320742328No Hit
TTTGGTAATGGGGCGTCCTTAAAAATACAGTAAAAAAGGCTGGGCACAGTGG4800.12917532320742328No Hit
CTTTGTAATGGGGTAAGACGGGCAATTACACTATAGAAGTCTGCATTCACAG4700.12648417064060197No Hit
ATGAGTAATGGGGGCATTATTTACAGTAGCCAAAAACTGGAAATAACTCAAA4630.12460036384382704No Hit
TCTTGTAATGGGGGTTGAGGTAATGGGGTGACCAACATGATGAAACCCCGTT4580.12325478756041637No Hit
TAACGTAATGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGG4510.12137098076364145No Hit
TGTGGTAATGGGGGCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAGAC4360.11733425191340949No Hit
CGTCGTAATGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC4300.1157195603733167No Hit
GTTCGTAATGGGGGACTTTGGATCCAGCTCTTCTCAGCTCTGCAGCATCAGA4290.11545044511663456No Hit
CGGAGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGGTTAGTCGCGG4260.11464309934658816No Hit
TTTTGTAATGGGGAGCAGAATACCCACCTTGTTTTCTTGCAAAGGGCAAGAT4230.11383575357654177No Hit
GCGGGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG4200.11302840780649537No Hit
TGGGGTAATGGGGGCTTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCC4190.11275929254981323No Hit
TGGTGTAATGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGA4160.11195194677976684No Hit
CCGGGTAATGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGT4120.11087548575303831No Hit
TCTTGTAATGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGT4110.11060637049635617No Hit
AGTCGTAATGGGGGCTTCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCA3980.10710787215948846No Hit
GCTTGTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAT3930.1057622958760778No Hit
TTGGGTAATGGGTATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCAT3900.10495495010603142No Hit
GCAGGTAATGGGGCGGAGCACCGGCCTTGCTTGTGAGAACTGAGCCTTGGTC3860.10387848907930289No Hit
GACAGTAATGGGGAGCAGTGCCACGTTCGGGCTACGGCCCTGAATGGAATCC3820.10280202805257437No Hit
GCATGTAATGGGGGCCTTTCCTACTTGGGCCCGGCAGAATGGCTCCCGCAAA3790.10199468228252796No Hit
GGCCGTAATGGGGATTGAAACAATGCATATTTTATTTAATAGCTCCCGTTCT3780.10172556702584583No Hit
AAGGGTAATGGGGTGTCGTCCCCACCTCTGTGTGATGCGCAAAGTACACCTG3740.1006491059991173No Hit
AGCTGTAATGGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATG3720.10011087548575304No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAT750.046.00000430
TCGGCGC750.046.00000426
GACTCTG750.046.00000446
TTCCTCG750.046.00000422
GCATAAG206.3059136E-446.032
TCAAAAC206.3059136E-446.015
TCCGGAC253.4126617E-546.019
TATTTTA405.5933924E-946.028
CCTTCAG206.3059136E-446.042
ATCCTTA600.046.015
CTTGTAC206.3059136E-446.035
ATTTAGG800.046.014
TATTTAC551.8189894E-1246.018
ACAACAT405.5933924E-946.022
ATCCTAT206.3059136E-446.019
GGAATTA301.8579794E-646.012
AACGTAT253.4126617E-546.026
AGCAATA405.5933924E-946.015
CGGGTAG253.4126617E-546.034
TTAGGGC551.8189894E-1246.032
Produced by FastQC (version 0.11.5)