##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528022_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	371588
Sequences flagged as poor quality	0
Sequence length	52
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.433832631839564	31.0	31.0	33.0	27.0	34.0
2	31.40663584399927	31.0	31.0	34.0	28.0	34.0
3	31.891194010570846	33.0	31.0	34.0	30.0	34.0
4	35.64683197519834	37.0	35.0	37.0	33.0	37.0
5	35.83484396697418	37.0	35.0	37.0	35.0	37.0
6	33.50714770121748	35.0	33.0	35.0	28.0	37.0
7	34.85867681410595	35.0	35.0	37.0	32.0	37.0
8	35.569275649375115	37.0	35.0	37.0	33.0	37.0
9	37.872681572063684	39.0	37.0	39.0	35.0	39.0
10	37.57932710421219	39.0	37.0	39.0	35.0	39.0
11	38.11726697309924	39.0	39.0	39.0	37.0	39.0
12	38.17850414975726	39.0	39.0	39.0	37.0	39.0
13	37.93311678525679	39.0	38.0	39.0	35.0	39.0
14	38.15395276489015	40.0	38.0	40.0	34.0	40.0
15	37.455512556917874	39.0	36.0	40.0	33.0	40.0
16	37.60016738968966	40.0	37.0	40.0	33.0	40.0
17	37.61761144062779	40.0	37.0	40.0	33.0	40.0
18	37.69210254367741	40.0	37.0	40.0	33.0	40.0
19	37.77013520350496	40.0	37.0	40.0	33.0	40.0
20	37.69130865367019	40.0	37.0	40.0	33.0	40.0
21	37.83433803029161	40.0	37.0	40.0	34.0	40.0
22	37.781677556864054	40.0	37.0	40.0	33.0	40.0
23	37.61640849543042	40.0	37.0	40.0	33.0	40.0
24	37.738411897047264	40.0	37.0	40.0	33.0	40.0
25	37.67648040302701	40.0	37.0	40.0	33.0	40.0
26	37.54203849424631	40.0	37.0	40.0	33.0	40.0
27	37.289193945983186	39.0	37.0	40.0	33.0	40.0
28	36.68819229899781	39.0	35.0	40.0	31.0	40.0
29	36.500589362412136	39.0	35.0	40.0	31.0	40.0
30	36.36807701002185	39.0	35.0	40.0	31.0	40.0
31	36.26476097182901	39.0	35.0	40.0	31.0	40.0
32	36.06635036653498	38.0	35.0	40.0	30.0	40.0
33	35.82216594723188	38.0	35.0	40.0	30.0	40.0
34	35.55410024005081	38.0	35.0	40.0	29.0	40.0
35	35.27658320505506	38.0	35.0	40.0	26.0	40.0
36	34.498778216734664	37.0	34.0	40.0	23.0	40.0
37	34.51856357040593	38.0	34.0	40.0	22.0	40.0
38	34.271074415750775	37.0	34.0	40.0	21.0	40.0
39	33.79033499467152	37.0	33.0	40.0	18.0	40.0
40	33.20230739421079	37.0	33.0	40.0	15.0	40.0
41	32.49913883117862	36.0	32.0	40.0	10.0	40.0
42	31.635397267941915	35.0	30.0	40.0	9.0	40.0
43	31.24028763038634	35.0	30.0	40.0	9.0	40.0
44	30.880485914507464	35.0	28.0	40.0	8.0	40.0
45	30.328901902106633	35.0	24.0	40.0	8.0	40.0
46	30.126026674704242	35.0	23.0	39.0	8.0	40.0
47	29.855883397741586	35.0	23.0	39.0	8.0	40.0
48	29.492712358849047	35.0	22.0	39.0	8.0	40.0
49	29.144057396901946	35.0	20.0	39.0	8.0	40.0
50	28.683361680140372	35.0	18.0	38.0	8.0	40.0
51	28.381866475774245	35.0	18.0	38.0	8.0	40.0
52	27.808406084157724	34.0	15.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	0.0
15	4.0
16	12.0
17	48.0
18	121.0
19	329.0
20	670.0
21	1106.0
22	1815.0
23	2800.0
24	4037.0
25	5714.0
26	7678.0
27	9654.0
28	12562.0
29	15625.0
30	17599.0
31	16410.0
32	14385.0
33	15463.0
34	20556.0
35	29222.0
36	34936.0
37	59004.0
38	80798.0
39	21037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.11123071789186	20.617727160188164	36.616898285197585	6.654143836722391
2	30.10700022605682	18.5162061207574	34.29039689118055	17.086396762005233
3	22.99293841566466	21.593539080917576	36.246864807259655	19.16665769615811
4	26.13055319332164	21.872342486840264	32.11917500026912	19.877929319568985
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	98.5284777764621	0.9653164257188068	0.33693230136602903	0.16927349645306092
12	97.66946187713273	1.4376137011959482	0.3937156205259589	0.4992088011453545
13	87.02137851599083	8.702380055330098	2.4508326426041744	1.8254087860749002
14	29.53674499714738	47.0615305122878	11.543698935380045	11.858025555184774
15	22.626134320806916	48.79570922634746	16.530135526443264	12.04802092640236
16	31.464417580761488	35.5638502858004	19.304175592322682	13.667556541115427
17	15.275789315047849	39.1438905454428	21.117743307103567	24.462576832405784
18	16.97632862202224	45.96461672605143	22.842772102436033	14.216282549490295
19	22.309116548435366	40.436720238543764	25.92764028978331	11.326522923237563
20	18.26054662690937	40.389355953367705	27.69115256682132	13.6589448529016
21	25.84582925175194	37.67344478293164	17.188929674801123	19.2917962905153
22	23.896896562859943	43.043101499510215	18.962668331593054	14.097333606036793
23	18.3671162685555	42.985241719323554	23.724124568070014	14.923517444050937
24	18.101768625466917	49.25562720001722	17.56353811210265	15.07906606241321
25	18.94060088054512	41.632668439239154	23.428905131489714	15.997825548726007
26	17.75487905960365	45.38198219533462	15.512610740928123	21.350528004133608
27	19.552838089496973	50.3568468303605	14.555098657653097	15.535216422489423
28	20.82144740949654	46.608071304778406	15.064533838552375	17.505947447172677
29	24.34093673638546	42.53555012540771	13.383101714802415	19.740411423404417
30	27.36713779777603	43.85044726955661	13.250158778001442	15.532256154665921
31	20.75228478852923	46.91378623636931	13.736719162082736	18.59720981301872
32	21.77465364866465	51.9744986382768	13.920793997653316	12.330053715405235
33	23.267166862223753	50.5210071369366	13.256617544161815	12.955208456677825
34	18.35231492943798	54.36208919556067	15.113512815268523	12.172083059732824
35	21.64628567122727	50.3902171221891	15.962302334843967	12.001194871739669
36	18.485257866238953	48.338213289987834	19.80769023757495	13.368838606198263
37	18.677406159509996	50.460994434696495	17.856604626629494	13.00499477916402
38	20.1548489186949	49.858176259728516	17.54846765772845	12.438507163848133
39	20.400551148045682	47.79675339354338	17.405029225916877	14.397666232494052
40	25.34688956586327	45.60723166517756	16.522331183999484	12.523547584959688
41	26.149391261289384	44.73529823352745	16.25671442565422	12.858596079528942
42	21.25687589480823	45.82144740949654	18.453502265950462	14.46817442974477
43	21.98294885733662	49.92491684338569	14.739173493223678	13.352960806054018
44	22.676727989063156	45.31631807270418	19.145397590880222	12.861556347352444
45	25.423318298760993	41.40257489477593	19.431467108733326	13.742639697729745
46	30.923765030087086	40.29731853558242	14.71306931332551	14.065847121004985
47	30.36992583183526	40.40334994671518	14.285983400970967	14.940740820478595
48	27.86096429378774	42.350667944067084	14.226239813987535	15.562127948157636
49	25.349042487916723	44.0915745395438	13.791080443932527	16.768302528606952
50	22.67322949072629	47.15383704532977	14.669472641743006	15.503460822200932
51	21.589771467324027	47.49776634336954	15.819671248802436	15.092790940504
52	21.90248339558866	46.698763146280285	16.045458949158746	15.353294508972304
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	11.5
6	22.0
7	85.0
8	148.0
9	612.5
10	1077.0
11	1679.0
12	2281.0
13	2620.5
14	2813.5
15	2667.0
16	2744.5
17	2822.0
18	3356.5
19	3891.0
20	4977.0
21	6063.0
22	7944.0
23	9825.0
24	12199.5
25	14574.0
26	16552.0
27	18530.0
28	19378.0
29	20226.0
30	19360.0
31	18494.0
32	17681.0
33	16868.0
34	15821.0
35	14774.0
36	15064.0
37	15354.0
38	15121.0
39	16024.0
40	17160.0
41	18281.0
42	19402.0
43	20850.5
44	22299.0
45	22941.5
46	23584.0
47	23206.0
48	22828.0
49	20504.5
50	18181.0
51	17172.0
52	16163.0
53	14984.5
54	13806.0
55	12535.5
56	11265.0
57	11732.0
58	12199.0
59	11146.0
60	10093.0
61	8382.5
62	6672.0
63	5604.0
64	4029.5
65	3523.0
66	2940.0
67	2357.0
68	1524.0
69	691.0
70	621.5
71	552.0
72	566.0
73	580.0
74	320.5
75	61.0
76	67.5
77	74.0
78	83.0
79	92.0
80	47.0
81	2.0
82	2.0
83	2.0
84	1.5
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	371588.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.166615826535136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.40219087137075	39.80965711747835
2	5.852616686615307	5.520962456751064
3	2.4624710486826946	3.484392778115452
4	1.515511379853674	2.859261721372016
5	1.0349696543203015	2.440800803872377
6	0.7684692601508735	2.1747656620822764
7	0.6227549813473011	2.0561271471488443
8	0.49957516444682354	1.8850615886353153
9	0.3869932685292797	1.6427846541758118
>10	2.214620763632481	17.675121679265395
>50	0.09080825165989762	3.041288281985436
>100	0.14028710673099568	14.507414600688676
>500	0.008149458482298503	2.5580647217891403
>1k	5.82104177307036E-4	0.3442967866398607
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCGGTAATGGGGTGGTGGCTCATGCCTCTAATCCCAGCACTTTGGGAGGCT	1254	0.3374705318793933	No Hit
GAGAGTAATGGGGCACTTTGGGAGGCTGAGGTGGGCAGATCACAAGGTCAGG	925	0.24893161243097192	No Hit
TCTCGTAATGGGGGCAGCCACAGGTCCCAGAGCTCCCAAGTCACACTCCCTT	912	0.24543311409410423	No Hit
ACTGGTAATGGGGGCCCCCACTGGCTGCTCCGAAAAGCCATCTTTGCATTGT	838	0.22551858509962647	No Hit
ACTAGTAATGGGGGGCGCGGTGGCTCAGGACTGTAATCCCAGCACTTTGGGA	775	0.20856432392865215	No Hit
AAGGGTAATGGGGATGAAGACTGCCGGGCAGAGGTGGTGCACACCTTTAATC	719	0.19349386955445277	No Hit
CATTGTAATGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGA	698	0.187842449164128	No Hit
GTTGGTAATGGGGCTCAATGGCCTGGAAATCGCCATCCTGGCCGAGAACCTC	666	0.1792307609502998	No Hit
TGTGGTAATGGGGATTTAGGTTGCTGAGTATTTTTTATTTGCCCATCCATTC	652	0.17546314735674995	No Hit
GCGAGTAATGGGGCATATTTTGCTTTTTCTTGAAGAAGAGTATTTGATCAGT	579	0.15581773361895432	No Hit
TTTTGTAATGGGTACCCTTCCAATATGACCAGTTTCTTATAGGAAAAGAAAA	518	0.13940170296134427	No Hit
GGGCGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGCGGGCTAACGG	515	0.1385943571912979	No Hit
CATTGTAATGGGGGCTTTTTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCG	512	0.1377870114212515	No Hit
CGCAGTAATGGGGAGGAATAAATAACACATGAAAAATAACCAGAAAGATGGT	507	0.13644143513784082	No Hit
ACAGGTAATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAA	501	0.13482674359774804	No Hit
CAAGGTAATGGGGACCTCACGCAAGATGGCGGAGCTGGAGGAGGTGACTCTG	492	0.13240470628760886	No Hit
GTCTGTAATGGGGCATCCTTACCTGGGAAACAGAGCAGCAGGAGCCCCAGGA	480	0.12917532320742328	No Hit
TTTGGTAATGGGGCGTCCTTAAAAATACAGTAAAAAAGGCTGGGCACAGTGG	480	0.12917532320742328	No Hit
CTTTGTAATGGGGTAAGACGGGCAATTACACTATAGAAGTCTGCATTCACAG	470	0.12648417064060197	No Hit
ATGAGTAATGGGGGCATTATTTACAGTAGCCAAAAACTGGAAATAACTCAAA	463	0.12460036384382704	No Hit
TCTTGTAATGGGGGTTGAGGTAATGGGGTGACCAACATGATGAAACCCCGTT	458	0.12325478756041637	No Hit
TAACGTAATGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGG	451	0.12137098076364145	No Hit
TGTGGTAATGGGGGCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAGAC	436	0.11733425191340949	No Hit
CGTCGTAATGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	430	0.1157195603733167	No Hit
GTTCGTAATGGGGGACTTTGGATCCAGCTCTTCTCAGCTCTGCAGCATCAGA	429	0.11545044511663456	No Hit
CGGAGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGGTTAGTCGCGG	426	0.11464309934658816	No Hit
TTTTGTAATGGGGAGCAGAATACCCACCTTGTTTTCTTGCAAAGGGCAAGAT	423	0.11383575357654177	No Hit
GCGGGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG	420	0.11302840780649537	No Hit
TGGGGTAATGGGGGCTTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCC	419	0.11275929254981323	No Hit
TGGTGTAATGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGA	416	0.11195194677976684	No Hit
CCGGGTAATGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGT	412	0.11087548575303831	No Hit
TCTTGTAATGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGT	411	0.11060637049635617	No Hit
AGTCGTAATGGGGGCTTCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCA	398	0.10710787215948846	No Hit
GCTTGTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAT	393	0.1057622958760778	No Hit
TTGGGTAATGGGTATTACCGTGGTGACGGATAAAAACTGCAATATAGCGCAT	390	0.10495495010603142	No Hit
GCAGGTAATGGGGCGGAGCACCGGCCTTGCTTGTGAGAACTGAGCCTTGGTC	386	0.10387848907930289	No Hit
GACAGTAATGGGGAGCAGTGCCACGTTCGGGCTACGGCCCTGAATGGAATCC	382	0.10280202805257437	No Hit
GCATGTAATGGGGGCCTTTCCTACTTGGGCCCGGCAGAATGGCTCCCGCAAA	379	0.10199468228252796	No Hit
GGCCGTAATGGGGATTGAAACAATGCATATTTTATTTAATAGCTCCCGTTCT	378	0.10172556702584583	No Hit
AAGGGTAATGGGGTGTCGTCCCCACCTCTGTGTGATGCGCAAAGTACACCTG	374	0.1006491059991173	No Hit
AGCTGTAATGGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATG	372	0.10011087548575304	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.07158465827744706	0.0	0.0	0.0	0.0
12	0.13724878090788722	0.0	0.0	0.0	0.0
13	0.28956801618997385	0.0	0.0	0.0	0.0
14	0.710464277640828	0.0	0.0	0.0	0.0
15	1.0382466602796645	0.0	0.0	0.0	0.0
16	1.2317405298341173	0.0	0.0	0.0	0.0
17	1.33938663250697	0.0	0.0	0.0	0.0
18	1.4992410949761563	0.0	0.0	0.0	0.0
19	1.6429486420444148	0.0	0.0	0.0	0.0
20	1.7987663756633692	0.0	0.0	0.0	0.0
21	1.9707310246832515	0.0	0.0	0.0	0.0
22	2.159649934874108	0.0	0.0	0.0	0.0
23	2.361486377385707	0.0	0.0	0.0	0.0
24	2.574625660677955	0.0	0.0	0.0	0.0
25	2.817367622205238	0.0	0.0	0.0	0.0
26	3.097516604411337	0.0	0.0	0.0	0.0
27	3.366900976350151	0.0	0.0	0.0	0.0
28	3.6381691550857402	0.0	0.0	0.0	0.0
29	3.915626984725018	0.0	0.0	0.0	0.0
30	4.2501372487809075	0.0	0.0	0.0	0.0
31	4.554506604088399	0.0	0.0	0.0	0.0
32	4.795095643562225	0.0	0.0	0.0	0.0
33	4.965983831555379	0.0	0.0	0.0	0.0
34	5.1374102500618966	0.0	0.0	0.0	0.0
35	5.285423641237069	0.0	0.0	0.0	0.0
36	5.433437032412241	0.0	0.0	0.0	0.0
37	5.580373962560685	0.0	0.0	0.0	0.0
38	5.750723920040475	0.0	0.0	0.0	0.0
39	5.9245723758571325	0.0	0.0	0.0	0.0
40	6.100573753727247	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAT	75	0.0	46.000004	30
TCGGCGC	75	0.0	46.000004	26
GACTCTG	75	0.0	46.000004	46
TTCCTCG	75	0.0	46.000004	22
GCATAAG	20	6.3059136E-4	46.0	32
TCAAAAC	20	6.3059136E-4	46.0	15
TCCGGAC	25	3.4126617E-5	46.0	19
TATTTTA	40	5.5933924E-9	46.0	28
CCTTCAG	20	6.3059136E-4	46.0	42
ATCCTTA	60	0.0	46.0	15
CTTGTAC	20	6.3059136E-4	46.0	35
ATTTAGG	80	0.0	46.0	14
TATTTAC	55	1.8189894E-12	46.0	18
ACAACAT	40	5.5933924E-9	46.0	22
ATCCTAT	20	6.3059136E-4	46.0	19
GGAATTA	30	1.8579794E-6	46.0	12
AACGTAT	25	3.4126617E-5	46.0	26
AGCAATA	40	5.5933924E-9	46.0	15
CGGGTAG	25	3.4126617E-5	46.0	34
TTAGGGC	55	1.8189894E-12	46.0	32
>>END_MODULE