SRR1528021_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1528021_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	384862
Sequences flagged as poor quality	0
Sequence length	59
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGCGGTAATGGGGTGGTGGCTCATGCCTCTAATCCCAGCACTTTGGGAGGCTGAGGTGG	1274	0.3310277450098997	No Hit
GAGAGTAATGGGGCACTTTGGGAGGCTGAGGTGGGCAGATCACAAGGTCAGGAGTTCAA	989	0.25697522748413715	No Hit
TCTCGTAATGGGGGCAGCCACAGGTCCCAGAGCTCCCAAGTCACACTCCCTTCTCACCA	899	0.23359022194968584	No Hit
ACTGGTAATGGGGGCCCCCACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGG	856	0.2224173859721147	No Hit
CATTGTAATGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACA	763	0.19825288025318166	No Hit
ACTAGTAATGGGGGGCGCGGTGGCTCAGGACTGTAATCCCAGCACTTTGGGAGGCTGAG	727	0.18889887803940114	No Hit
GTTGGTAATGGGGCTCAATGGCCTGGAAATCGCCATCCTGGCCGAGAACCTCAATAACC	698	0.18136370958941125	No Hit
AAGGGTAATGGGGATGAAGACTGCCGGGCAGAGGTGGTGCACACCTTTAATCCCAAATG	676	0.17564737490321206	No Hit
TGTGGTAATGGGGATTTAGGTTGCTGAGTATTTTTTATTTGCCCATCCATTCTTTCGTT	669	0.1738285411394214	No Hit
GCGAGTAATGGGGCATATTTTGCTTTTTCTTGAAGAAGAGTATTTGATCAGTATTTCAG	642	0.166813039479086	No Hit
GGGCGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGCGGGCTAACGGCATTTTT	590	0.1533017029480697	No Hit
ATGAGTAATGGGGGCATTATTTACAGTAGCCAAAAACTGGAAATAACTCAAATGTCCAT	576	0.14966403542048837	No Hit
CAAGGTAATGGGGACCTCACGCAAGATGGCGGAGCTGGAGGAGGTGACTCTGGACGGGA	526	0.13667236567912655	No Hit
GTCTGTAATGGGGCATCCTTACCTGGGAAACAGAGCAGCAGGAGCCCCAGGAGCTGAGC	526	0.13667236567912655	No Hit
ACAGGTAATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC	523	0.13589286549464483	No Hit
CGCAGTAATGGGGAGGAATAAATAACACATGAAAAATAACCAGAAAGATGGTAGAGTGA	505	0.13121586438775457	No Hit
TTTTGTAATGGGTACCCTTCCAATATGACCAGTTTCTTATAGGAAAAGAAAACAGAGGC	488	0.12679869667569155	No Hit
TTTGGTAATGGGGCGTCCTTAAAAATACAGTAAAAAAGGCTGGGCACAGTGGCTCATGC	488	0.12679869667569155	No Hit
TCTTGTAATGGGGGTTGAGGTAATGGGGTGACCAACATGATGAAACCCCGTTTCTACTA	455	0.11822419464639274	No Hit
GCGGGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAG	451	0.11718486106708377	No Hit
TAACGTAATGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGA	446	0.1158856940929476	No Hit
CATTGTAATGGGGGCTTTTTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTC	445	0.11562586069812036	No Hit
CCGGGTAATGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCTC	435	0.113027526749848	No Hit
TTTTGTAATGGGGAGCAGAATACCCACCTTGTTTTCTTGCAAAGGGCAAGATATCCTTT	434	0.11276769335502076	No Hit
TGTGGTAATGGGGGCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAGACCCGGAAA	432	0.11224802656536628	No Hit
GGCCGTAATGGGGATTGAAACAATGCATATTTTATTTAATAGCTCCCGTTCTTGTTTTC	429	0.11146852638088457	No Hit
GCTTGTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTT	425	0.11042919280157563	No Hit
TGGTGTAATGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCT	423	0.10990952601192115	No Hit
GTTCGTAATGGGGGACTTTGGATCCAGCTCTTCTCAGCTCTGCAGCATCAGAATTAAAA	419	0.1088701924326122	No Hit
CTTTGTAATGGGGTAAGACGGGCAATTACACTATAGAAGTCTGCATTCACAGAAGATGA	419	0.1088701924326122	No Hit
AGCTGTAATGGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATGCACGCTC	418	0.10861035903778496	No Hit
TCTTGTAATGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCTC	398	0.10341369114124023	No Hit
TGGGGTAATGGGGGCTTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAG	398	0.10341369114124023	No Hit
CGTCGTAATGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG	391	0.10159485737744958	No Hit
CGGAGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGGTTAGTCGCGGGGTTTTC	390	0.10133502398262234	No Hit
GTAGGTAATGGGGGAGGGGGAGCCTACGGGCTCCCGCGAGAGCCTAGCTGAGCAGTTTA	390	0.10133502398262234	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGGA	45	7.6397555E-11	53.000004	16
AACAATG	65	0.0	53.000004	19
TGTCACC	65	0.0	53.000004	43
CTGTCAC	65	0.0	53.000004	42
TCTTGCG	45	7.6397555E-11	53.000004	27
ACCGGCC	45	7.6397555E-11	53.000004	20
TCTCTCA	45	7.6397555E-11	53.000004	53
ACTGAGC	45	7.6397555E-11	53.000004	39
CCTAGCT	45	7.6397555E-11	53.000004	43
TTTAATA	65	0.0	53.000004	35
CACGCGT	45	7.6397555E-11	53.000004	47
CCGGACG	45	7.6397555E-11	53.000004	28
GCCGGAC	45	7.6397555E-11	53.000004	27
CCTACGG	45	7.6397555E-11	53.000004	23
CTAAGTG	45	7.6397555E-11	53.000004	31
TACGGGC	45	7.6397555E-11	53.000004	25
CTTGGGT	45	7.6397555E-11	53.000004	29
GCTCTAG	45	7.6397555E-11	53.000004	14
CTACGGG	45	7.6397555E-11	53.000004	24
CGAGGTC	45	7.6397555E-11	53.000004	39

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content