##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528021_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	384862
Sequences flagged as poor quality	0
Sequence length	59
%GC	40
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.266950231511554	31.0	31.0	33.0	27.0	34.0
2	31.272874432913614	31.0	31.0	34.0	28.0	34.0
3	31.69247938222012	31.0	31.0	34.0	30.0	34.0
4	35.500491085116224	37.0	35.0	37.0	33.0	37.0
5	35.58913064942759	37.0	35.0	37.0	33.0	37.0
6	33.09657227785544	35.0	32.0	35.0	28.0	37.0
7	34.54218135331625	35.0	35.0	37.0	32.0	37.0
8	35.34628776028811	37.0	35.0	37.0	33.0	37.0
9	37.629352858946845	39.0	37.0	39.0	35.0	39.0
10	37.203163731415415	39.0	37.0	39.0	33.0	39.0
11	37.910625107181275	39.0	37.0	39.0	35.0	39.0
12	38.00004417167712	39.0	38.0	39.0	35.0	39.0
13	37.65130878080974	39.0	37.0	39.0	35.0	39.0
14	37.5334587462519	40.0	37.0	40.0	32.0	40.0
15	36.91952440095411	39.0	36.0	40.0	31.0	40.0
16	37.11916219320172	39.0	36.0	40.0	32.0	40.0
17	37.019895443041925	39.0	36.0	40.0	32.0	40.0
18	37.04518242902651	39.0	36.0	40.0	32.0	40.0
19	37.09208755346072	39.0	36.0	40.0	32.0	40.0
20	37.09779609314508	39.0	36.0	40.0	32.0	40.0
21	37.19435537933077	39.0	36.0	40.0	32.0	40.0
22	37.13884197452593	39.0	36.0	40.0	32.0	40.0
23	37.00920589717873	39.0	36.0	40.0	31.0	40.0
24	37.1058015600397	39.0	36.0	40.0	32.0	40.0
25	36.957504248276	39.0	36.0	40.0	31.0	40.0
26	36.742941625829516	39.0	36.0	40.0	31.0	40.0
27	36.46617748699534	39.0	35.0	40.0	30.0	40.0
28	36.104019102951185	38.0	35.0	40.0	30.0	40.0
29	35.782911796955794	38.0	35.0	40.0	30.0	40.0
30	35.56401515348359	38.0	34.0	40.0	29.0	40.0
31	35.4625580078054	38.0	34.0	40.0	29.0	40.0
32	35.204353768363724	38.0	34.0	40.0	27.0	40.0
33	34.87390025515639	37.0	34.0	40.0	25.0	40.0
34	34.5988795984015	37.0	33.0	40.0	24.0	40.0
35	34.36609485997578	37.0	33.0	40.0	23.0	40.0
36	33.856590154393004	36.0	33.0	40.0	21.0	40.0
37	33.6857393039583	36.0	33.0	40.0	21.0	40.0
38	33.35282516850196	36.0	33.0	40.0	18.0	40.0
39	32.81920532554526	35.0	32.0	40.0	15.0	40.0
40	32.21778715487629	35.0	31.0	40.0	12.0	40.0
41	31.513030644750586	35.0	30.0	39.0	10.0	40.0
42	30.828884119502575	35.0	28.0	39.0	9.0	40.0
43	30.37820569450868	35.0	26.0	39.0	8.0	40.0
44	30.018416991025354	35.0	24.0	39.0	8.0	40.0
45	29.652426064407504	35.0	23.0	39.0	8.0	40.0
46	29.23236640666005	35.0	22.0	38.0	8.0	40.0
47	28.908975165124122	35.0	20.0	38.0	8.0	40.0
48	28.508416003658454	34.0	20.0	38.0	8.0	40.0
49	28.20475391179176	34.0	18.0	38.0	8.0	40.0
50	27.75854981785679	33.0	17.0	37.0	8.0	40.0
51	27.383566057443968	33.0	15.0	37.0	8.0	40.0
52	27.021875373510504	33.0	13.0	37.0	8.0	40.0
53	26.579867588901998	33.0	11.0	36.0	8.0	40.0
54	26.15387333641669	33.0	10.0	36.0	8.0	39.0
55	25.64580031283941	32.0	10.0	35.0	8.0	39.0
56	25.12877602881033	31.0	10.0	35.0	8.0	39.0
57	24.594756042425598	31.0	10.0	35.0	8.0	38.0
58	24.108108880585768	30.0	9.0	35.0	8.0	38.0
59	23.44251445972842	28.0	9.0	35.0	8.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	2.0
15	21.0
16	80.0
17	249.0
18	613.0
19	1296.0
20	2258.0
21	3627.0
22	5382.0
23	7529.0
24	9929.0
25	12670.0
26	15925.0
27	18665.0
28	17760.0
29	14617.0
30	13872.0
31	15464.0
32	18449.0
33	22009.0
34	27383.0
35	31521.0
36	36264.0
37	49047.0
38	53511.0
39	6718.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.57362898909219	20.890345110715007	36.041490196486016	6.494535703706783
2	29.768332545172036	18.752176104681677	34.259292941366	17.22019840878029
3	23.010845445900088	21.632429286341598	36.0575998669653	19.29912540079301
4	26.133263351538993	21.974889700723896	32.104494598063724	19.78735234967339
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	98.5766326631364	0.9434550566176968	0.313878740951302	0.16603353929460432
12	97.66799528142555	1.4173911687825766	0.3832542573701742	0.5313592924216992
13	86.96493808170202	8.73377990032791	2.4738737521501215	1.8274082658199562
14	29.14837006511425	47.46636456703961	11.499446554869017	11.885818812977119
15	22.527035664731773	49.41173719411114	16.336764866367684	11.724462274789405
16	31.742806512464206	35.695651947970966	19.027859336593377	13.533682202971455
17	15.07553356787628	39.54092635801924	20.810056591713394	24.57348348239109
18	17.00453669107368	46.3490809692825	22.527035664731773	14.119346674912048
19	22.336058119533757	40.651454287510845	25.819904277377347	11.192583315578052
20	18.239264983292713	40.36407855283192	27.84036875555394	13.556287708321424
21	26.063368168330463	37.69948708887861	17.059881204171884	19.177263538619037
22	23.971709339971213	43.077778528407585	18.950948651724513	13.99956347989669
23	18.086482947134296	43.17729471862642	23.930915496983335	14.805306837255952
24	18.024902432560243	49.72613560185209	17.440017460804132	14.808944504783533
25	18.74334176925755	41.964392431572875	23.57884124699243	15.713424552177143
26	17.550186820210882	45.7223628209592	15.574933352734227	21.152517006095692
27	19.425144597284223	50.848875700900585	14.538717774163207	15.18726192765199
28	20.43121950205528	47.34450270486564	15.04513306068149	17.179144732397585
29	24.3045559187449	42.76467928764077	13.308926316445897	19.621838477168442
30	27.319922465714985	44.031886754213204	13.184466120323648	15.463724659748168
31	20.4390145039001	47.54717275283088	13.539138704262827	18.47467403900619
32	21.635287453684697	52.72305397778945	13.673472569388508	11.968185999137352
33	23.01292411305871	51.210303953105274	13.1324994413582	12.644272492477823
34	17.997360092708554	55.314892091191126	14.876241354043787	11.81150646205653
35	21.49523725387282	51.13703093576398	15.828011079295957	11.53972073106724
36	17.728692362457192	49.84747779723641	19.8608852004095	12.562944639896898
37	18.28265716022886	51.41505266822913	17.604491999729774	12.697798171812233
38	19.951567055204205	50.545390295742365	17.392467949550745	12.110574699502678
39	20.098892590071245	48.7398080350879	17.067676206016703	14.093623168824148
40	24.668842338292688	46.662180210049314	16.427186887767565	12.241790563890435
41	25.579558387162148	45.98167654899679	15.84438058317007	12.594384480670994
42	20.259729461469306	47.46896290098789	18.184959803773822	14.086347833768986
43	21.242159527311088	51.4166116685981	14.376321902396183	12.964906901694635
44	21.64723978984675	46.86562975819905	18.928343146374544	12.558787305579663
45	24.36016026523793	42.89693448560783	19.12971402736565	13.61319122178859
46	30.263834829107576	41.36573628989092	14.24796420535153	14.122464675649974
47	29.934106251071814	41.36183878896851	13.915377459972667	14.788677499987008
48	27.234956945606477	43.31916375220209	13.800531099459027	15.645348202732409
49	24.670921005451305	45.228419537392625	13.326594987294147	16.774064469861923
50	21.698686802022543	48.54181498822955	14.319938055718673	15.439560154029236
51	21.09353482546991	48.62807967531219	15.304966455508728	14.973419043709175
52	21.074307154252693	47.99174769138029	15.56064251601873	15.37330263834829
53	20.04510707734201	47.582769927922214	16.250500179285044	16.121622815450735
54	20.742499909058314	47.3946505500673	15.619624696644513	16.243224844229882
55	20.172165607412527	44.88128212190344	17.905379070939713	17.041173199744325
56	21.053520482666514	44.48555586158155	17.13003622077524	17.330887434976695
57	21.53057459556932	42.322962516434465	17.79287121097952	18.3535916770167
58	21.6181384496261	41.16410557550499	18.33540333937879	18.882352635490125
59	21.88394801253436	39.85688376612916	19.741881505578622	18.517286715757855
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	16.0
5	32.0
6	82.0
7	132.0
8	456.5
9	1153.0
10	1525.0
11	1812.5
12	2100.0
13	1879.5
14	1500.5
15	1342.0
16	1345.0
17	1348.0
18	1554.5
19	2406.0
20	3051.0
21	4091.0
22	5131.0
23	6782.0
24	8433.0
25	10744.5
26	14820.0
27	16584.0
28	17960.5
29	19337.0
30	19532.0
31	20257.5
32	20788.0
33	20089.0
34	19390.0
35	20415.0
36	21227.5
37	21015.0
38	20148.5
39	19282.0
40	18639.0
41	17996.0
42	18517.0
43	17926.5
44	16815.0
45	16974.0
46	17133.0
47	16856.0
48	16264.5
49	15950.0
50	15459.0
51	14968.0
52	13104.5
53	11261.5
54	11282.0
55	9724.0
56	8166.0
57	9077.5
58	10063.0
59	10137.0
60	7965.0
61	5793.0
62	4726.0
63	3659.0
64	3359.5
65	2817.5
66	2575.0
67	1693.0
68	811.0
69	722.5
70	606.0
71	578.0
72	339.0
73	100.0
74	175.0
75	157.5
76	65.0
77	65.5
78	66.0
79	52.0
80	38.0
81	30.5
82	12.0
83	1.0
84	1.0
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	384862.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.02854050395762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.37697078487082	50.076612277411726
2	4.252691024543154	4.765441426388663
3	1.7600759350538422	2.9584345745161587
4	1.0018635753824698	2.245318156510261
5	0.6652950425497972	1.8637755119291763
6	0.45219546027299207	1.520151099696666
7	0.34549014138035217	1.3550115866032977
8	0.27769309234265854	1.2446990937591913
9	0.23774144892053464	1.1988275760282374
>10	1.4489414188600427	13.63027283678522
>50	0.059258617601971715	2.338238974229923
>100	0.11431780561009854	13.812707536162176
>500	0.006999049323067257	2.657446240278007
>1k	4.6660328820448383E-4	0.3330631097013459
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCGGTAATGGGGTGGTGGCTCATGCCTCTAATCCCAGCACTTTGGGAGGCTGAGGTGG	1274	0.3310277450098997	No Hit
GAGAGTAATGGGGCACTTTGGGAGGCTGAGGTGGGCAGATCACAAGGTCAGGAGTTCAA	989	0.25697522748413715	No Hit
TCTCGTAATGGGGGCAGCCACAGGTCCCAGAGCTCCCAAGTCACACTCCCTTCTCACCA	899	0.23359022194968584	No Hit
ACTGGTAATGGGGGCCCCCACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGG	856	0.2224173859721147	No Hit
CATTGTAATGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACA	763	0.19825288025318166	No Hit
ACTAGTAATGGGGGGCGCGGTGGCTCAGGACTGTAATCCCAGCACTTTGGGAGGCTGAG	727	0.18889887803940114	No Hit
GTTGGTAATGGGGCTCAATGGCCTGGAAATCGCCATCCTGGCCGAGAACCTCAATAACC	698	0.18136370958941125	No Hit
AAGGGTAATGGGGATGAAGACTGCCGGGCAGAGGTGGTGCACACCTTTAATCCCAAATG	676	0.17564737490321206	No Hit
TGTGGTAATGGGGATTTAGGTTGCTGAGTATTTTTTATTTGCCCATCCATTCTTTCGTT	669	0.1738285411394214	No Hit
GCGAGTAATGGGGCATATTTTGCTTTTTCTTGAAGAAGAGTATTTGATCAGTATTTCAG	642	0.166813039479086	No Hit
GGGCGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGCGGGCTAACGGCATTTTT	590	0.1533017029480697	No Hit
ATGAGTAATGGGGGCATTATTTACAGTAGCCAAAAACTGGAAATAACTCAAATGTCCAT	576	0.14966403542048837	No Hit
CAAGGTAATGGGGACCTCACGCAAGATGGCGGAGCTGGAGGAGGTGACTCTGGACGGGA	526	0.13667236567912655	No Hit
GTCTGTAATGGGGCATCCTTACCTGGGAAACAGAGCAGCAGGAGCCCCAGGAGCTGAGC	526	0.13667236567912655	No Hit
ACAGGTAATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC	523	0.13589286549464483	No Hit
CGCAGTAATGGGGAGGAATAAATAACACATGAAAAATAACCAGAAAGATGGTAGAGTGA	505	0.13121586438775457	No Hit
TTTTGTAATGGGTACCCTTCCAATATGACCAGTTTCTTATAGGAAAAGAAAACAGAGGC	488	0.12679869667569155	No Hit
TTTGGTAATGGGGCGTCCTTAAAAATACAGTAAAAAAGGCTGGGCACAGTGGCTCATGC	488	0.12679869667569155	No Hit
TCTTGTAATGGGGGTTGAGGTAATGGGGTGACCAACATGATGAAACCCCGTTTCTACTA	455	0.11822419464639274	No Hit
GCGGGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAG	451	0.11718486106708377	No Hit
TAACGTAATGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGA	446	0.1158856940929476	No Hit
CATTGTAATGGGGGCTTTTTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTC	445	0.11562586069812036	No Hit
CCGGGTAATGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCTC	435	0.113027526749848	No Hit
TTTTGTAATGGGGAGCAGAATACCCACCTTGTTTTCTTGCAAAGGGCAAGATATCCTTT	434	0.11276769335502076	No Hit
TGTGGTAATGGGGGCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAGACCCGGAAA	432	0.11224802656536628	No Hit
GGCCGTAATGGGGATTGAAACAATGCATATTTTATTTAATAGCTCCCGTTCTTGTTTTC	429	0.11146852638088457	No Hit
GCTTGTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTT	425	0.11042919280157563	No Hit
TGGTGTAATGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCT	423	0.10990952601192115	No Hit
GTTCGTAATGGGGGACTTTGGATCCAGCTCTTCTCAGCTCTGCAGCATCAGAATTAAAA	419	0.1088701924326122	No Hit
CTTTGTAATGGGGTAAGACGGGCAATTACACTATAGAAGTCTGCATTCACAGAAGATGA	419	0.1088701924326122	No Hit
AGCTGTAATGGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATGCACGCTC	418	0.10861035903778496	No Hit
TCTTGTAATGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCTC	398	0.10341369114124023	No Hit
TGGGGTAATGGGGGCTTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAG	398	0.10341369114124023	No Hit
CGTCGTAATGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG	391	0.10159485737744958	No Hit
CGGAGTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGGTTAGTCGCGGGGTTTTC	390	0.10133502398262234	No Hit
GTAGGTAATGGGGGAGGGGGAGCCTACGGGCTCCCGCGAGAGCCTAGCTGAGCAGTTTA	390	0.10133502398262234	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.06755668265508157	0.0	0.0	0.0	0.0
12	0.12394052933259195	0.0	0.0	0.0	0.0
13	0.2686677302513628	0.0	0.0	0.0	0.0
14	0.652961321200846	0.0	0.0	0.0	0.0
15	0.962422894440085	0.0	0.0	0.0	0.0
16	1.121440932074354	0.0	0.0	0.0	0.0
17	1.2256341234000758	0.0	0.0	0.0	0.0
18	1.3737391584516008	0.0	0.0	0.0	0.0
19	1.5083328569721095	0.0	0.0	0.0	0.0
20	1.6418872219133092	0.0	0.0	0.0	0.0
21	1.7998659259682692	0.0	0.0	0.0	0.0
22	1.9640806314990829	0.0	0.0	0.0	0.0
23	2.131153504372996	0.0	0.0	0.0	0.0
24	2.338760386839958	0.0	0.0	0.0	0.0
25	2.5603982726275913	0.0	0.0	0.0	0.0
26	2.81061783184622	0.0	0.0	0.0	0.0
27	3.0634357250131217	0.0	0.0	0.0	0.0
28	3.3102774500989964	0.0	0.0	0.0	0.0
29	3.5859606820106946	0.0	0.0	0.0	0.0
30	3.9016582567257876	0.0	0.0	0.0	0.0
31	4.168247319818533	0.0	0.0	0.0	0.0
32	4.367279700256196	0.0	0.0	0.0	0.0
33	4.53799024065769	0.0	0.0	0.0	0.0
34	4.704543446741949	0.0	0.0	0.0	0.0
35	4.840696145631421	0.0	0.0	0.0	0.0
36	4.964636674964013	0.0	0.0	0.0	0.0
37	5.097671373115558	0.0	0.0	0.0	0.0
38	5.241878907244675	0.0	0.0	0.0	0.0
39	5.394141276613436	0.0	0.0	0.0	0.0
40	5.551860147273568	0.0	0.0	0.0	0.0
41	5.7264681885974715	0.0	0.0	0.0	0.0
42	5.9044540640541285	0.0	0.0	0.0	0.0
43	6.089975107960775	0.0	0.0	0.0	0.0
44	6.27575598526225	0.0	0.0	0.0	0.0
45	6.461536862563724	0.0	0.0	0.0	0.0
46	6.641601405178999	0.0	0.0	0.0	0.0
47	6.8076349444736035	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGGA	45	7.6397555E-11	53.000004	16
AACAATG	65	0.0	53.000004	19
TGTCACC	65	0.0	53.000004	43
CTGTCAC	65	0.0	53.000004	42
TCTTGCG	45	7.6397555E-11	53.000004	27
ACCGGCC	45	7.6397555E-11	53.000004	20
TCTCTCA	45	7.6397555E-11	53.000004	53
ACTGAGC	45	7.6397555E-11	53.000004	39
CCTAGCT	45	7.6397555E-11	53.000004	43
TTTAATA	65	0.0	53.000004	35
CACGCGT	45	7.6397555E-11	53.000004	47
CCGGACG	45	7.6397555E-11	53.000004	28
GCCGGAC	45	7.6397555E-11	53.000004	27
CCTACGG	45	7.6397555E-11	53.000004	23
CTAAGTG	45	7.6397555E-11	53.000004	31
TACGGGC	45	7.6397555E-11	53.000004	25
CTTGGGT	45	7.6397555E-11	53.000004	29
GCTCTAG	45	7.6397555E-11	53.000004	14
CTACGGG	45	7.6397555E-11	53.000004	24
CGAGGTC	45	7.6397555E-11	53.000004	39
>>END_MODULE