FastQCFastQC Report
Fri 17 Jun 2016
SRR1528020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160865
Sequences flagged as poor quality0
Sequence length52
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAGCATGGGTATCGCAATAGCTTCGACTTTCACTTCTTCAATGCCCGT14260.8864575886612998No Hit
TCTTTAGCATGGGCATAAGATCATCTGCAATACTAGTTAAAATGCTGCTTCC7650.47555403599291335No Hit
TTTCTAGCATGGGGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGG7360.45752649737357415No Hit
TGCTTAGCATGGGCTGGGCATGGTGGCGTGTGCCTGTAATCCCAGCTACTCG6370.3959842103627266No Hit
CTCATAGCATGGCCTATAAAGAACCAGGCGTTTCCCCCTGGAAGCTCTCTCG6280.390389456998104No Hit
TACGTAGCATGGGATCGTGTGGTTGGCGTGCTGGAGGATTCCAGTGTCGTCG5160.32076585957169057No Hit
CCTTTAGCATGGGAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCA5040.31330618841886054No Hit
GGTTTAGCATGGGGAAGAGCTGCAGTCCTTCGTTGGAGCTCTGCAGTCCTTC4860.30211668168961553No Hit
ATTTTAGCATGGGTATCAACGCAGAGTTCTCTAGCATAGGGCAACAGCGTAA4710.29279209274857804No Hit
CCTATAGCATGGGGCTCAGGGCACCCCAAGAAGGCCTGGGCTTCTCACTATG3980.24741242656886206No Hit
AATTTAGCATAGGCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCAGAT3710.23062816647499454No Hit
ATGATAGCATGGGGCATGGGTACAAAAATTAGCTGGGCGAGGTGGTGCACGC3680.22876324868678707No Hit
CCTCTAGCATGGGATCTTGGATATGATATGGCGCACTACACATGCTAGCCGC3600.2237901345849004No Hit
TCACTAGCATGGGAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAGTGCTG3550.22068193827122123No Hit
GTCATAGCATGGGGCGCGGTGGCTTACACCTGTAATCCCAGCACTTTGGGAG3510.2181953812202779No Hit
CTGGTAGCATGGGTCACATCGACAAGTTAATTCACCATGATCAAGTAGGCTT3420.21260062785565534No Hit
AAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAGAAG3190.19830292481273115No Hit
TAGCTAGCATGGAGGTGGGGGATTGATACAGAGGGGGCTCATGGGAGCCCTC3160.19643800702452366No Hit
ATTCTAGCATGGGGGCAAGGATCGGTACGCCGGATTTGAACACGAGTTCGGG3130.19457308923631617No Hit
TGCTTAGCATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA3100.19270817144810867No Hit
CGAGTAGCATGGGAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTG2770.17219407577782614No Hit
GGCATAGCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT2740.17032915798961862No Hit
TCGGTAGCATGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTT2720.16908587946414697No Hit
AAGTTAGCATGGGGCGGGTGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGC2670.1659776831504678No Hit
GATCTAGCATGGTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAG2630.16349112609952446No Hit
CTTCTAGCATGGGAACTTTAAAAACTTCTTTACAGCAAAAGAAACAATCAAC2590.16100456904858113No Hit
TGGTTAGCATGGATACACACAATCCTCCATTCTCCCTGTTTCCCTGTTCCTC2580.16038292978584529No Hit
CTTTTAGCATGGGTGGGAATTCAGATAAAACGAACAGCTCGTAACCAAACAT2510.15603145494669443No Hit
GACTTAGCATGGGGGGAATTCCTCTGTGTCATGATCGTGAGTTGTCGCAGTG2510.15603145494669443No Hit
TTAATAGCATGGGACTCCCAAATGTGTGAACCCATTCAAGTGCGCGAGCAAG2460.15292325863301526No Hit
CTTTTAGCATGGGTGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGA2430.15105834084480776No Hit
TTCTTAGCATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA2330.14484194821744942No Hit
CGCTTAGCATTTTTTTAAGGATAAGACAGAGAGAGAGAAAGAGGTTGGGTAT2320.14422030895471358No Hit
CTAATAGCATGGGGCAAAGGGTTTTTGTGAATTACGGTGGTGGGTAAGACCC2310.14359866969197776No Hit
TTTTTAGCATGATGGGGCTCCTGCCGCTCCTGGTGCTGCTTGTGCGCTCCCT2280.14173375190377024No Hit
CATATAGCATGGGGATAATGGGATTAAGATATGATCACAGAGAGACATAAAT2260.14049047337829856No Hit
ATTGTAGCATTGTGCCAATTCATCCATTAACTTCTCAGTAACAGATACAAAC2250.13986883411556272No Hit
GCACTAGCATGGGGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAG2160.13427408075094022No Hit
CAGATAGCATGGGTGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAAC2150.1336524414882044No Hit
TATTTAGCATGGGTCCACCTTGTTAGAAGTGACATCACTGTACATTAAAGAC2140.13303080222546856No Hit
TTGGTAGCATGGGGCCGTGGCAGACTCGACTTCGTGATGACACTGCTCTACA2130.13240916296273272No Hit
CCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGAAAAAGCGTCCTGCG2090.1299226059117894No Hit
TTCTTAGCATGGGGCTCTGTTCTCCCGGTTCCTTCGAGCTCGTCGTCTCTGC2070.1286793273863177No Hit
GGTGTAGCATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC2030.12619277033537438No Hit
CTTCTAGCATGGGAAATGCCCGGTGTGTAAAGCTGGCATCAGCAGGGAGAAG2030.12619277033537438No Hit
GCAATAGCATGGGAGTGATGGACCTTTGTTCTGTGGTTTTTGTTTTTGTGTT2010.12494949180990272No Hit
GGCTTAGCATGGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGA2000.12432785254716688No Hit
ATGGTAGCATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA1880.11686818139433686No Hit
GGTTTAGCATGAGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT1790.11127342802971435No Hit
GCTTTAGCATGGGGGGGAAGGTGCTATATTGCTTATTGTGGACAAGGTTTCG1780.11065178876697851No Hit
TCGTTAGCATGGGGCCATCTATCCCTGGCGCGGGGAGGCGATACAACGACGA1710.10630031392782767No Hit
TTCTTAGCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT1680.10443539613962019No Hit
TGCTTAGCATGGGGAGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAA1670.10381375687688434No Hit
TTAATAGCATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT1650.10257047835141267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACC351.0122494E-746.00000434
TAGAAGC351.0122494E-746.00000436
GCGGGCA351.0122494E-746.00000443
GAGGCCA351.0122494E-746.00000434
TGTGAAC351.0122494E-746.00000425
ACCGGGA351.0122494E-746.00000426
TGGTCAC351.0122494E-746.00000410
ATCTCGA351.0122494E-746.00000432
GGCACCG351.0122494E-746.00000423
CCAAATG351.0122494E-746.00000418
ATTAGGC351.0122494E-746.00000419
GATACAC351.0122494E-746.00000412
TTCAAGT351.0122494E-746.00000435
GCGGCTA351.0122494E-746.00000413
ATAGGCT351.0122494E-746.0000049
CCGCTAG351.0122494E-746.0000041
CCGCTAC351.0122494E-746.00000440
CTCCATT351.0122494E-746.00000425
GATGCCC351.0122494E-746.00000441
ACACCTG351.0122494E-746.00000426
Produced by FastQC (version 0.11.5)