FastQCFastQC Report
Fri 17 Jun 2016
SRR1528019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159267
Sequences flagged as poor quality0
Sequence length52
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAGCATGGGTATCGCAATAGCTTCGACTTTCACTTCTTCAATGCCCGT13460.8451217138515826No Hit
TCTTTAGCATGGGCATAAGATCATCTGCAATACTAGTTAAAATGCTGCTTCC7720.48472062636955554No Hit
TTTCTAGCATGGGGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGG7330.46023344446746656No Hit
CTCATAGCATGGCCTATAAAGAACCAGGCGTTTCCCCCTGGAAGCTCTCTCG5990.37609799895772505No Hit
TGCTTAGCATGGGCTGGGCATGGTGGCGTGTGCCTGTAATCCCAGCTACTCG5750.361028963941055No Hit
TACGTAGCATGGGATCGTGTGGTTGGCGTGCTGGAGGATTCCAGTGTCGTCG4730.29698556512020696No Hit
GGTTTAGCATGGGGAAGAGCTGCAGTCCTTCGTTGGAGCTCTGCAGTCCTTC4570.2869395417757602No Hit
ATTTTAGCATGGGTATCAACGCAGAGTTCTCTAGCATAGGGCAACAGCGTAA4420.2775213948903414No Hit
CCTTTAGCATGGGAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCA4340.27249838321811803No Hit
AATTTAGCATAGGCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCAGAT4230.2655917421688109No Hit
CCTATAGCATGGGGCTCAGGGCACCCCAAGAAGGCCTGGGCTTCTCACTATG3860.24236031318477777No Hit
GTCATAGCATGGGGCGCGGTGGCTTACACCTGTAATCCCAGCACTTTGGGAG3600.22603552525005177No Hit
ATGATAGCATGGGGCATGGGTACAAAAATTAGCTGGGCGAGGTGGTGCACGC3560.2235240194139401No Hit
CCTCTAGCATGGGATCTTGGATATGATATGGCGCACTACACATGCTAGCCGC3530.22164039003685637No Hit
CTGGTAGCATGGGTCACATCGACAAGTTAATTCACCATGATCAAGTAGGCTT3460.2172452548236609No Hit
TCACTAGCATGGGAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAGTGCTG3120.19589745521671154No Hit
TAGCTAGCATGGAGGTGGGGGATTGATACAGAGGGGGCTCATGGGAGCCCTC3040.19087444354448818No Hit
CGAGTAGCATGGGAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTG2840.17831691436392974No Hit
AAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAGAAG2820.17706116144587392No Hit
ATTCTAGCATGGGGGCAAGGATCGGTACGCCGGATTTGAACACGAGTTCGGG2760.1732939026917064No Hit
TGCTTAGCATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA2730.1714102733146226No Hit
GATCTAGCATGGTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAG2730.1714102733146226No Hit
AAGTTAGCATGGGGCGGGTGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGC2600.16324787934725965No Hit
CTTTTAGCATGGGTGGGAATTCAGATAAAACGAACAGCTCGTAACCAAACAT2570.16136424997017587No Hit
GACTTAGCATGGGGGGAATTCCTCTGTGTCATGATCGTGAGTTGTCGCAGTG2520.15822486767503627No Hit
GCACTAGCATGGGGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAG2390.15006247370767328No Hit
GGCATAGCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT2390.15006247370767328No Hit
CTTCTAGCATGGGAACTTTAAAAACTTCTTTACAGCAAAAGAAACAATCAAC2360.14817884433058953No Hit
CTTCTAGCATGGGAAATGCCCGGTGTGTAAAGCTGGCATCAGCAGGGAGAAG2350.14755096787156158No Hit
TCGGTAGCATGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTT2330.14629521495350575No Hit
TTAATAGCATGGGACTCCCAAATGTGTGAACCCATTCAAGTGCGCGAGCAAG2280.14315583265836615No Hit
CCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGAAAAAGCGTCCTGCG2260.1419000797403103No Hit
CGCTTAGCATTTTTTTAAGGATAAGACAGAGAGAGAGAAAGAGGTTGGGTAT2260.1419000797403103No Hit
GGTGTAGCATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC2230.14001645036322655No Hit
CAGATAGCATGGGTGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAAC2120.1331098093139194No Hit
GGCTTAGCATGGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGA2070.12997042701877978No Hit
TATTTAGCATGGGTCCACCTTGTTAGAAGTGACATCACTGTACATTAAAGAC2060.12934255055975186No Hit
TTTTTAGCATGATGGGGCTCCTGCCGCTCCTGGTGCTGCTTGTGCGCTCCCT2060.12934255055975186No Hit
ATTGTAGCATTGTGCCAATTCATCCATTAACTTCTCAGTAACAGATACAAAC2040.12808679764169603No Hit
CTTTTAGCATGGGTGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGA2010.12620316826461225No Hit
TTCTTAGCATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA1990.1249474153465564No Hit
TTGGTAGCATGGGGCCGTGGCAGACTCGACTTCGTGATGACACTGCTCTACA1990.1249474153465564No Hit
TGCTTAGCATGGGGAGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAA1950.12243590951044472No Hit
CTAATAGCATGGGGCAAAGGGTTTTTGTGAATTACGGTGGTGGGTAAGACCC1910.11992440367433303No Hit
CATATAGCATGGGGATAATGGGATTAAGATATGATCACAGAGAGACATAAAT1890.1186686507562772No Hit
ATGGTAGCATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA1870.11741289783822136No Hit
TGGTTAGCATGGATACACACAATCCTCCATTCTCCCTGTTTCCCTGTTCCTC1860.11678502137919343No Hit
GCAATAGCATGGGAGTGATGGACCTTTGTTCTGTGGTTTTTGTTTTTGTGTT1840.11552926846113759No Hit
TTCTTAGCATGGGGCTCTGTTCTCCCGGTTCCTTCGAGCTCGTCGTCTCTGC1800.11301776262502589No Hit
GAAGTAGCATGGGAAGACCTATGTAAAGAAACGGGTCACTCCATCGATGTTC1660.104227492198635No Hit
CACATAGCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT1650.10359961573960708No Hit
GGTTTAGCATGAGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT1640.10297173928057915No Hit
GCTTTAGCATGGGGGGGAAGGTGCTATATTGCTTATTGTGGACAAGGTTTCG1610.10108810990349537No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAG351.01215846E-746.00000420
TTGTCGC351.01215846E-746.00000442
TGGCATC351.01215846E-746.00000434
CCAATTC351.01215846E-746.00000415
GGATGCT351.01215846E-746.00000411
ACACGAG351.01215846E-746.00000440
CAATGCC1050.046.00000443
CATGAGG351.01215846E-746.0000048
GGCATCA351.01215846E-746.00000435
CCTATAA1050.046.00000413
GGCATAA1050.046.00000412
CTATAAA1050.046.00000414
CACATCG351.01215846E-746.00000415
TACAGCA351.01215846E-746.00000431
TCGACAA351.01215846E-746.00000419
GATACAG351.01215846E-746.00000425
GCCTATA1050.046.00000412
ACCATGA351.01215846E-746.00000434
TAAAATG1050.046.00000438
GGCGCAG351.01215846E-746.00000412
Produced by FastQC (version 0.11.5)