##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528019_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	159267
Sequences flagged as poor quality	0
Sequence length	52
%GC	39
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.206131841498866	31.0	31.0	34.0	28.0	34.0
2	31.770982061569566	33.0	31.0	34.0	30.0	34.0
3	32.04651936684938	33.0	31.0	34.0	30.0	34.0
4	35.795952708345105	37.0	35.0	37.0	35.0	37.0
5	35.48504712212825	37.0	35.0	37.0	33.0	37.0
6	35.83689653223831	37.0	35.0	37.0	35.0	37.0
7	35.67393747606221	37.0	35.0	37.0	33.0	37.0
8	35.423063158093015	37.0	35.0	37.0	32.0	37.0
9	37.156887490817304	39.0	37.0	39.0	33.0	39.0
10	37.75390382188401	39.0	37.0	39.0	35.0	39.0
11	38.25973993357067	39.0	39.0	39.0	37.0	39.0
12	38.23077599251571	39.0	39.0	39.0	37.0	39.0
13	38.17027381692378	39.0	39.0	39.0	37.0	39.0
14	38.543584044403424	40.0	39.0	40.0	35.0	40.0
15	37.806212209685626	40.0	37.0	40.0	33.0	40.0
16	36.568215637891086	38.0	35.0	40.0	31.0	40.0
17	36.2792920065048	38.0	35.0	40.0	31.0	40.0
18	36.24545574412779	39.0	35.0	40.0	30.0	40.0
19	36.548789140248765	39.0	35.0	40.0	31.0	40.0
20	36.67406305135402	39.0	35.0	40.0	31.0	40.0
21	36.8093955433329	39.0	35.0	40.0	32.0	40.0
22	36.85971356275939	39.0	35.0	40.0	32.0	40.0
23	36.820697319595396	39.0	35.0	40.0	32.0	40.0
24	36.859638217584305	39.0	35.0	40.0	32.0	40.0
25	36.8830266156831	39.0	35.0	40.0	32.0	40.0
26	36.64923681616405	39.0	35.0	40.0	31.0	40.0
27	36.395850992358746	39.0	35.0	40.0	31.0	40.0
28	35.72943547627569	38.0	35.0	40.0	30.0	40.0
29	35.48445691825676	37.0	35.0	40.0	29.0	40.0
30	35.16931944470606	36.0	35.0	40.0	29.0	40.0
31	35.04013386326106	35.0	34.0	40.0	28.0	40.0
32	34.88255570833883	35.0	34.0	40.0	26.0	40.0
33	34.60311929024845	35.0	34.0	40.0	25.0	40.0
34	34.33017511474442	35.0	33.0	40.0	23.0	40.0
35	33.974257065179856	35.0	33.0	40.0	21.0	40.0
36	33.259733654806084	35.0	33.0	40.0	18.0	40.0
37	33.13499971745559	35.0	33.0	40.0	18.0	40.0
38	32.772470128777464	35.0	33.0	40.0	16.0	40.0
39	32.23087645274916	35.0	32.0	40.0	15.0	40.0
40	31.52525005179981	35.0	30.0	40.0	10.0	40.0
41	30.673981427414343	35.0	26.0	40.0	9.0	40.0
42	29.650756277194898	35.0	22.0	39.0	8.0	40.0
43	28.837342324524226	35.0	18.0	39.0	8.0	40.0
44	28.002982413180384	35.0	15.0	39.0	8.0	40.0
45	27.099210759291	34.0	10.0	38.0	8.0	40.0
46	26.701224986971564	33.0	10.0	38.0	8.0	40.0
47	26.272611400980743	33.0	10.0	38.0	8.0	40.0
48	25.849121286895592	33.0	10.0	37.0	8.0	40.0
49	25.451688045860095	33.0	9.0	37.0	8.0	40.0
50	25.040177814613198	32.0	9.0	36.0	8.0	40.0
51	24.74931404496851	31.0	9.0	36.0	8.0	40.0
52	24.07844060602636	30.0	9.0	35.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	0.0
15	6.0
16	28.0
17	67.0
18	181.0
19	373.0
20	690.0
21	1023.0
22	1401.0
23	1949.0
24	2644.0
25	3562.0
26	4720.0
27	6096.0
28	7260.0
29	8734.0
30	10389.0
31	10386.0
32	8558.0
33	7033.0
34	7764.0
35	12248.0
36	10339.0
37	17518.0
38	25409.0
39	10887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.118392385114305	24.738332485700113	37.15835672173144	5.984918407454149
2	28.10688968838491	21.771616216793184	35.55224873953801	14.569245355283893
3	18.390501484927828	22.398864799362077	37.27828112540577	21.932352590304333
4	19.70904204888646	19.693345137410763	38.773882850810274	21.8237299628925
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	98.15655471629402	1.057971833462048	0.5167423257799796	0.2687311244639505
12	98.04102544783288	1.242567512416257	0.3993294279417582	0.31707761180910043
13	95.26267211663433	2.235240194139401	1.3285865873030822	1.1735011019231856
14	58.64366127320788	20.173042752108095	11.719314107756158	9.463981866927863
15	41.61753533374773	32.439237255677575	17.883177306033264	8.06005010454143
16	39.01247590524088	33.20775804152775	17.699209503538082	10.080556549693283
17	25.542014353255855	40.36492179798703	16.46794376738433	17.62512008137279
18	19.771201818330226	49.18407454149322	18.242950517056265	12.801773123120293
19	20.498910634343588	50.41534027764697	19.398871078126668	9.686878009882776
20	16.895527635982344	48.7790942254202	21.45579435790214	12.86958378069531
21	17.93215166983744	48.68805213886116	14.402858093641496	18.976938097659904
22	19.535120269735724	52.11060671702236	15.351579423232684	13.00269359000923
23	15.555011395957733	53.824709450168584	16.06610283360646	14.554176320267223
24	16.034709010655064	53.098256387073285	14.344465582951898	16.522569019319757
25	20.07258251866363	52.96075144254616	12.01818330225345	14.948482736536759
26	17.272881387858124	56.589249499268526	13.484902710542674	12.652966402330678
27	16.918759064966377	53.652671300394935	13.311608807850966	16.11696082678772
28	15.248607683952105	55.37305279813144	13.131408264109954	16.2469312538065
29	17.562332435469997	55.29080098199878	15.19272667909862	11.954139903432601
30	20.62260229677209	57.5467610992861	12.075320060024989	9.755316543916818
31	17.15044547834768	57.05387807894918	11.886957122316613	13.90871932038652
32	17.663420545373494	59.17986776921773	14.257190755147017	8.899520930261762
33	17.171165401495603	57.24161314019853	15.801138967896676	9.786082490409187
34	14.732493234631153	61.23616317253418	13.700264335989251	10.331079256845422
35	18.731438402179986	58.860278651572514	11.838610634971463	10.569672311276033
36	17.022358680705985	57.73763554283059	12.726427948036944	12.513577828426477
37	15.3496957938556	59.93080801421512	13.016506872107843	11.702989319821432
38	15.610264524352187	57.149315300721426	16.037848392950202	11.202571781976179
39	16.93006084122888	55.95886153440449	14.257818631606046	12.853258992760585
40	20.088279430139323	55.18217835458695	12.845096598793221	11.884445616480502
41	20.964794966942303	51.81801628711534	15.129311156736799	12.087877589205547
42	22.18601467975161	51.14116546428325	13.206753439193305	13.466066416771834
43	23.061902340095564	48.772187584370904	13.696497077235085	14.469412998298456
44	24.933602064457798	46.65436028806972	12.402443695178537	16.009593952293947
45	23.761356715452667	44.73682558219845	15.083476175227762	16.418341527121125
46	24.23854282431389	41.927706304507524	19.079281960481456	14.75446891069713
47	27.524848210866033	43.608531585325274	14.75132952840199	14.115290675406708
48	26.245235987367128	40.91117431734132	14.44680944577345	18.396780249518105
49	25.47294794276278	43.20292339279324	13.57971205585589	17.744416608588097
50	26.373322785008824	41.804014642079025	14.985527447619408	16.83713512529275
51	24.757168779470952	43.79689452303365	15.219725366836819	16.22621133065858
52	27.480896858734077	40.68325516271418	14.962296018635374	16.873551959916366
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	21.0
10	42.0
11	105.5
12	169.0
13	245.0
14	434.0
15	547.0
16	725.5
17	904.0
18	1203.5
19	1503.0
20	2099.0
21	2695.0
22	3635.5
23	4576.0
24	6052.0
25	7528.0
26	8865.0
27	10202.0
28	10472.0
29	10742.0
30	10665.5
31	10589.0
32	9955.5
33	9322.0
34	8657.0
35	7992.0
36	7687.0
37	7382.0
38	7846.0
39	8085.5
40	7861.0
41	8316.0
42	8771.0
43	8020.0
44	7269.0
45	7491.0
46	7713.0
47	7963.0
48	8213.0
49	7687.0
50	7161.0
51	7171.5
52	7182.0
53	6601.5
54	6021.0
55	5195.0
56	4369.0
57	4075.0
58	3781.0
59	3394.0
60	3007.0
61	2593.0
62	2179.0
63	1881.0
64	1037.5
65	492.0
66	424.5
67	357.0
68	280.5
69	204.0
70	182.0
71	160.0
72	106.0
73	52.0
74	48.0
75	44.0
76	27.5
77	11.0
78	8.0
79	5.0
80	4.5
81	4.0
82	2.5
83	1.0
84	1.0
85	1.0
86	1.5
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	159267.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.84750764439589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.2507481168094	54.91533085950009
2	4.0057785574244145	4.874832827892784
3	1.5086162418739038	2.7538661492964644
4	0.8771024662057579	2.1347799606949334
5	0.5984934475286349	1.8208417311809728
6	0.4540295119182747	1.6575938518337132
7	0.3085336910535549	1.3141454287454402
8	0.2713858218966051	1.3210520697947472
9	0.21979155917861934	1.2036391719565258
>10	1.3909813228768961	13.823328121958722
>50	0.025797131358992878	1.1414794025127617
>100	0.08358270560313694	10.511907677045466
>500	0.004127541017438861	1.6820810337358023
>1k	0.0010318852543597153	0.8451217138515826
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTAGCATGGGTATCGCAATAGCTTCGACTTTCACTTCTTCAATGCCCGT	1346	0.8451217138515826	No Hit
TCTTTAGCATGGGCATAAGATCATCTGCAATACTAGTTAAAATGCTGCTTCC	772	0.48472062636955554	No Hit
TTTCTAGCATGGGGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGG	733	0.46023344446746656	No Hit
CTCATAGCATGGCCTATAAAGAACCAGGCGTTTCCCCCTGGAAGCTCTCTCG	599	0.37609799895772505	No Hit
TGCTTAGCATGGGCTGGGCATGGTGGCGTGTGCCTGTAATCCCAGCTACTCG	575	0.361028963941055	No Hit
TACGTAGCATGGGATCGTGTGGTTGGCGTGCTGGAGGATTCCAGTGTCGTCG	473	0.29698556512020696	No Hit
GGTTTAGCATGGGGAAGAGCTGCAGTCCTTCGTTGGAGCTCTGCAGTCCTTC	457	0.2869395417757602	No Hit
ATTTTAGCATGGGTATCAACGCAGAGTTCTCTAGCATAGGGCAACAGCGTAA	442	0.2775213948903414	No Hit
CCTTTAGCATGGGAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCA	434	0.27249838321811803	No Hit
AATTTAGCATAGGCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCAGAT	423	0.2655917421688109	No Hit
CCTATAGCATGGGGCTCAGGGCACCCCAAGAAGGCCTGGGCTTCTCACTATG	386	0.24236031318477777	No Hit
GTCATAGCATGGGGCGCGGTGGCTTACACCTGTAATCCCAGCACTTTGGGAG	360	0.22603552525005177	No Hit
ATGATAGCATGGGGCATGGGTACAAAAATTAGCTGGGCGAGGTGGTGCACGC	356	0.2235240194139401	No Hit
CCTCTAGCATGGGATCTTGGATATGATATGGCGCACTACACATGCTAGCCGC	353	0.22164039003685637	No Hit
CTGGTAGCATGGGTCACATCGACAAGTTAATTCACCATGATCAAGTAGGCTT	346	0.2172452548236609	No Hit
TCACTAGCATGGGAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAGTGCTG	312	0.19589745521671154	No Hit
TAGCTAGCATGGAGGTGGGGGATTGATACAGAGGGGGCTCATGGGAGCCCTC	304	0.19087444354448818	No Hit
CGAGTAGCATGGGAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTG	284	0.17831691436392974	No Hit
AAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAGAAG	282	0.17706116144587392	No Hit
ATTCTAGCATGGGGGCAAGGATCGGTACGCCGGATTTGAACACGAGTTCGGG	276	0.1732939026917064	No Hit
TGCTTAGCATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA	273	0.1714102733146226	No Hit
GATCTAGCATGGTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAG	273	0.1714102733146226	No Hit
AAGTTAGCATGGGGCGGGTGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGC	260	0.16324787934725965	No Hit
CTTTTAGCATGGGTGGGAATTCAGATAAAACGAACAGCTCGTAACCAAACAT	257	0.16136424997017587	No Hit
GACTTAGCATGGGGGGAATTCCTCTGTGTCATGATCGTGAGTTGTCGCAGTG	252	0.15822486767503627	No Hit
GCACTAGCATGGGGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAG	239	0.15006247370767328	No Hit
GGCATAGCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	239	0.15006247370767328	No Hit
CTTCTAGCATGGGAACTTTAAAAACTTCTTTACAGCAAAAGAAACAATCAAC	236	0.14817884433058953	No Hit
CTTCTAGCATGGGAAATGCCCGGTGTGTAAAGCTGGCATCAGCAGGGAGAAG	235	0.14755096787156158	No Hit
TCGGTAGCATGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTT	233	0.14629521495350575	No Hit
TTAATAGCATGGGACTCCCAAATGTGTGAACCCATTCAAGTGCGCGAGCAAG	228	0.14315583265836615	No Hit
CCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGAAAAAGCGTCCTGCG	226	0.1419000797403103	No Hit
CGCTTAGCATTTTTTTAAGGATAAGACAGAGAGAGAGAAAGAGGTTGGGTAT	226	0.1419000797403103	No Hit
GGTGTAGCATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC	223	0.14001645036322655	No Hit
CAGATAGCATGGGTGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAAC	212	0.1331098093139194	No Hit
GGCTTAGCATGGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGA	207	0.12997042701877978	No Hit
TATTTAGCATGGGTCCACCTTGTTAGAAGTGACATCACTGTACATTAAAGAC	206	0.12934255055975186	No Hit
TTTTTAGCATGATGGGGCTCCTGCCGCTCCTGGTGCTGCTTGTGCGCTCCCT	206	0.12934255055975186	No Hit
ATTGTAGCATTGTGCCAATTCATCCATTAACTTCTCAGTAACAGATACAAAC	204	0.12808679764169603	No Hit
CTTTTAGCATGGGTGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGA	201	0.12620316826461225	No Hit
TTCTTAGCATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA	199	0.1249474153465564	No Hit
TTGGTAGCATGGGGCCGTGGCAGACTCGACTTCGTGATGACACTGCTCTACA	199	0.1249474153465564	No Hit
TGCTTAGCATGGGGAGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAA	195	0.12243590951044472	No Hit
CTAATAGCATGGGGCAAAGGGTTTTTGTGAATTACGGTGGTGGGTAAGACCC	191	0.11992440367433303	No Hit
CATATAGCATGGGGATAATGGGATTAAGATATGATCACAGAGAGACATAAAT	189	0.1186686507562772	No Hit
ATGGTAGCATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA	187	0.11741289783822136	No Hit
TGGTTAGCATGGATACACACAATCCTCCATTCTCCCTGTTTCCCTGTTCCTC	186	0.11678502137919343	No Hit
GCAATAGCATGGGAGTGATGGACCTTTGTTCTGTGGTTTTTGTTTTTGTGTT	184	0.11552926846113759	No Hit
TTCTTAGCATGGGGCTCTGTTCTCCCGGTTCCTTCGAGCTCGTCGTCTCTGC	180	0.11301776262502589	No Hit
GAAGTAGCATGGGAAGACCTATGTAAAGAAACGGGTCACTCCATCGATGTTC	166	0.104227492198635	No Hit
CACATAGCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	165	0.10359961573960708	No Hit
GGTTTAGCATGAGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	164	0.10297173928057915	No Hit
GCTTTAGCATGGGGGGGAAGGTGCTATATTGCTTATTGTGGACAAGGTTTCG	161	0.10108810990349537	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.040184093377786986	0.0	0.0	0.0	0.0
12	0.059020387148624634	0.0	0.0	0.0	0.0
13	0.09920448052641162	0.0	0.0	0.0	0.0
14	0.24675544839797323	0.0	0.0	0.0	0.0
15	0.48472062636955554	0.0	0.0	0.0	0.0
16	0.648596382175843	0.0	0.0	0.0	0.0
17	0.8143557673592144	0.0	0.0	0.0	0.0
18	0.9631624881488319	0.0	0.0	0.0	0.0
19	1.1264103674960915	0.0	0.0	0.0	0.0
20	1.3154011816634958	0.0	0.0	0.0	0.0
21	1.4786490610107554	0.0	0.0	0.0	0.0
22	1.6958943158344164	0.0	0.0	0.0	0.0
23	1.907488682526826	0.0	0.0	0.0	0.0
24	2.157383513219939	0.0	0.0	0.0	0.0
25	2.4487181902088944	0.0	0.0	0.0	0.0
26	2.814142289363145	0.0	0.0	0.0	0.0
27	3.1613579712055855	0.0	0.0	0.0	0.0
28	3.5362002172452547	0.0	0.0	0.0	0.0
29	3.977597367941884	0.0	0.0	0.0	0.0
30	4.467341005983663	0.0	0.0	0.0	0.0
31	4.891785492286537	0.0	0.0	0.0	0.0
32	5.230210903702588	0.0	0.0	0.0	0.0
33	5.53159160403599	0.0	0.0	0.0	0.0
34	5.784625817024242	0.0	0.0	0.0	0.0
35	6.024474624372909	0.0	0.0	0.0	0.0
36	6.213465438540313	0.0	0.0	0.0	0.0
37	6.382364206018824	0.0	0.0	0.0	0.0
38	6.561308996841781	0.0	0.0	0.0	0.0
39	6.7515555639272415	0.0	0.0	0.0	0.0
40	6.926733095996032	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAAG	35	1.01215846E-7	46.000004	20
TTGTCGC	35	1.01215846E-7	46.000004	42
TGGCATC	35	1.01215846E-7	46.000004	34
CCAATTC	35	1.01215846E-7	46.000004	15
GGATGCT	35	1.01215846E-7	46.000004	11
ACACGAG	35	1.01215846E-7	46.000004	40
CAATGCC	105	0.0	46.000004	43
CATGAGG	35	1.01215846E-7	46.000004	8
GGCATCA	35	1.01215846E-7	46.000004	35
CCTATAA	105	0.0	46.000004	13
GGCATAA	105	0.0	46.000004	12
CTATAAA	105	0.0	46.000004	14
CACATCG	35	1.01215846E-7	46.000004	15
TACAGCA	35	1.01215846E-7	46.000004	31
TCGACAA	35	1.01215846E-7	46.000004	19
GATACAG	35	1.01215846E-7	46.000004	25
GCCTATA	105	0.0	46.000004	12
ACCATGA	35	1.01215846E-7	46.000004	34
TAAAATG	105	0.0	46.000004	38
GGCGCAG	35	1.01215846E-7	46.000004	12
>>END_MODULE