FastQCFastQC Report
Fri 17 Jun 2016
SRR1528018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166043
Sequences flagged as poor quality0
Sequence length59
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAGCATGGGTATCGCAATAGCTTCGACTTTCACTTCTTCAATGCCCGTTAGTCTA13770.8293032527718723No Hit
TCTTTAGCATGGGCATAAGATCATCTGCAATACTAGTTAAAATGCTGCTTCCTGGGTCC8140.49023445733936394No Hit
TTTCTAGCATGGGGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACT6540.39387387604415725No Hit
TGCTTAGCATGGGCTGGGCATGGTGGCGTGTGCCTGTAATCCCAGCTACTCGAGAGGTT6270.3776130279505911No Hit
CTCATAGCATGGCCTATAAAGAACCAGGCGTTTCCCCCTGGAAGCTCTCTCGTGCGCTC6040.3637611943894052No Hit
CCTTTAGCATGGGAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCAT4910.29570653384966544No Hit
TACGTAGCATGGGATCGTGTGGTTGGCGTGCTGGAGGATTCCAGTGTCGTCGCTGAGCT4840.29149075841800015No Hit
GGTTTAGCATGGGGAAGAGCTGCAGTCCTTCGTTGGAGCTCTGCAGTCCTTCGTTGGAG4790.28847949025252495No Hit
ATTTTAGCATGGGTATCAACGCAGAGTTCTCTAGCATAGGGCAACAGCGTAAATCCCTA4560.27462765669133904No Hit
ATGATAGCATGGGGCATGGGTACAAAAATTAGCTGGGCGAGGTGGTGCACGCCTGTAAT4030.24270821413730181No Hit
AATTTAGCATAGGCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCAGATCACAAGG3940.2372879314394464No Hit
CCTATAGCATGGGGCTCAGGGCACCCCAAGAAGGCCTGGGCTTCTCACTATGATACATA3810.22945863420921084No Hit
CTGGTAGCATGGGTCACATCGACAAGTTAATTCACCATGATCAAGTAGGCTTCATTCCT3770.22704961967683068No Hit
TCACTAGCATGGGAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAGTGCTGCCTTTGC3590.21620905428111994No Hit
GTCATAGCATGGGGCGCGGTGGCTTACACCTGTAATCCCAGCACTTTGGGAGGCCGAGG3480.2095842643170745No Hit
AAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAGAAGTCGTCAT3390.20416398161921911No Hit
CCTCTAGCATGGGATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAG3380.2035617279861241No Hit
TAGCTAGCATGGAGGTGGGGGATTGATACAGAGGGGGCTCATGGGAGCCCTCTGGTGTA3000.1806760899285125No Hit
ATTCTAGCATGGGGGCAAGGATCGGTACGCCGGATTTGAACACGAGTTCGGGCGCGCGC2990.18007383629541746No Hit
CTTCTAGCATGGGAACTTTAAAAACTTCTTTACAGCAAAAGAAACAATCAACAAAGTGA2950.1776648217630373No Hit
GGCATAGCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA2930.1764603144968472No Hit
CTTTTAGCATGGGTGGGAATTCAGATAAAACGAACAGCTCGTAACCAAACATGCACAGC2810.1692332708997067No Hit
CGAGTAGCATGGGAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAG2720.16381298820185133No Hit
GATCTAGCATGGTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTC2690.1620062273025662No Hit
ATTGTAGCATTGTGCCAATTCATCCATTAACTTCTCAGTAACAGATACAAACTCATCAC2620.1577904518709009No Hit
TGCTTAGCATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTG2600.15658594460471081No Hit
CCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGAAAAAGCGTCCTGCGTGTAGCG2550.1535746764392356No Hit
GACTTAGCATGGGGGGAATTCCTCTGTGTCATGATCGTGAGTTGTCGCAGTGTCTGTAC2450.1475521401082852No Hit
TCGGTAGCATGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCAAGA2430.14634763284209512No Hit
GCACTAGCATGGGGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCG2360.14213185741042983No Hit
ATGGTAGCATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTG2250.13550706744638435No Hit
CTTTTAGCATGGGTGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATT2210.1330980529140042No Hit
CATATAGCATGGGGATAATGGGATTAAGATATGATCACAGAGAGACATAAATTAAAGAA2210.1330980529140042No Hit
TTAATAGCATGGGACTCCCAAATGTGTGAACCCATTCAAGTGCGCGAGCAAGTCAGACG2190.1318935456478141No Hit
TGGTTAGCATGGATACACACAATCCTCCATTCTCCCTGTTTCCCTGTTCCTCTCCTACA2150.12948453111543395No Hit
TTGGTAGCATGGGGCCGTGGCAGACTCGACTTCGTGATGACACTGCTCTACACCAAAAA2100.12647326294995875No Hit
CTAATAGCATGGGGCAAAGGGTTTTTGTGAATTACGGTGGTGGGTAAGACCCGAGTTCC2100.12647326294995875No Hit
AAGTTAGCATGGGGCGGGTGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAA2080.12526875568376866No Hit
GGTGTAGCATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGT2070.12466650205067362No Hit
CGCTTAGCATTTTTTTAAGGATAAGACAGAGAGAGAGAAAGAGGTTGGGTATAGTGGGA2040.1228597411513885No Hit
TATTTAGCATGGGTCCACCTTGTTAGAAGTGACATCACTGTACATTAAAGACATCATAT2020.12165523388519842No Hit
TTTTTAGCATGATGGGGCTCCTGCCGCTCCTGGTGCTGCTTGTGCGCTCCCTCAGTGCG1980.11924621935281825No Hit
TTCTTAGCATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTG1960.11804171208662816No Hit
GCAATAGCATGGGAGTGATGGACCTTTGTTCTGTGGTTTTTGTTTTTGTGTTTGTTTGT1890.11382593665496288No Hit
TGCTTAGCATGGGGAGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAG1860.11201917575567776No Hit
CTTCTAGCATGGGAAATGCCCGGTGTGTAAAGCTGGCATCAGCAGGGAGAAGGTCGTCC1850.11141692212258271No Hit
GGTTTAGCATGAGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA1810.10900790759020254No Hit
TTAATAGCATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGAC1780.10720114669091742No Hit
GAAGTAGCATGGGAAGACCTATGTAAAGAAACGGGTCACTCCATCGATGTTCTTCTTCG1770.10659889305782237No Hit
GGCTTAGCATGGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCA1760.10599663942472734No Hit
GCTTTAGCATGGGGGGGAAGGTGCTATATTGCTTATTGTGGACAAGGTTTCGTAAGAAA1750.1053943857916323No Hit
CAGATAGCATGGGTGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATT1740.10479213215853725No Hit
GGTATAGCATGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAG1670.10057635672687196No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCA550.053.00000443
CGATAAG750.053.00000431
AACGACA550.053.00000440
GGTCCTC550.053.00000433
TAAGTCG750.053.00000434
GTACTAG1100.053.0000041
CACAAGG550.053.00000453
GATAAGT750.053.00000432
CGCCAGC550.053.00000419
CGGGCAG550.053.00000444
TGGGGCC750.053.00000410
GCTGTAA550.053.00000413
AGGCTGT550.053.00000411
GTCTTAC750.053.00000442
CTTACCG750.053.00000444
CCTCAAC550.053.00000436
CGTGTCT750.053.00000439
ATCACAA550.053.00000451
CAAGGCG550.053.00000439
GTGGCGA750.053.00000427
Produced by FastQC (version 0.11.5)