FastQCFastQC Report
Fri 17 Jun 2016
SRR1528017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4218999
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTT351.0204349E-746.00000414
CGATAAT700.046.00000427
GCGTACT351.0204349E-746.00000413
TAACTAA351.0204349E-746.00000419
ATTAGGT206.313381E-446.030
CGTATAA301.8625942E-646.034
AATCACG405.6170393E-946.014
CGACGTA453.110472E-1046.039
CGTCTAT253.4187196E-546.022
CGATTAG253.4187196E-546.036
ACGGGTA206.313381E-446.034
ATGTACG253.4187196E-546.027
ATATCGC301.8625942E-646.026
CCGTAAC551.8189894E-1246.015
TACGCCC453.110472E-1046.040
TACGATA501.6370905E-1146.044
TACGACT405.6170393E-946.032
CGTACTC405.6170393E-946.021
CGTACTA301.8625942E-646.017
CGTACGT301.8625942E-646.042
Produced by FastQC (version 0.11.5)