##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528017_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4218999
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16815007540888	31.0	31.0	33.0	30.0	33.0
2	31.997420715198082	33.0	31.0	34.0	30.0	34.0
3	32.37959264745026	34.0	31.0	34.0	30.0	34.0
4	36.06601755534903	37.0	35.0	37.0	35.0	37.0
5	36.142552771403835	37.0	35.0	37.0	35.0	37.0
6	36.24817166346804	37.0	37.0	37.0	35.0	37.0
7	36.183580749841376	37.0	37.0	37.0	35.0	37.0
8	36.14635959856829	37.0	37.0	37.0	35.0	37.0
9	37.7851087426188	39.0	38.0	39.0	35.0	39.0
10	34.777585157047916	35.0	34.0	37.0	32.0	39.0
11	37.30908658665243	37.0	37.0	39.0	35.0	39.0
12	38.12970588521116	39.0	39.0	39.0	37.0	39.0
13	38.191441619208724	39.0	39.0	39.0	37.0	39.0
14	38.75248868274204	40.0	39.0	40.0	36.0	40.0
15	38.179607769520686	40.0	38.0	40.0	34.0	40.0
16	37.69952374959084	40.0	37.0	40.0	33.0	40.0
17	37.73348796716947	40.0	37.0	40.0	33.0	40.0
18	37.72760908452455	40.0	37.0	40.0	33.0	40.0
19	37.8332426720177	40.0	37.0	40.0	33.0	40.0
20	37.848489890611496	40.0	37.0	40.0	33.0	40.0
21	37.65913525933521	40.0	37.0	40.0	33.0	40.0
22	37.706382722536794	40.0	37.0	40.0	33.0	40.0
23	37.63891861552942	40.0	37.0	40.0	33.0	40.0
24	37.63085414336434	40.0	37.0	40.0	33.0	40.0
25	37.722564997052615	40.0	37.0	40.0	33.0	40.0
26	37.710520670898475	40.0	37.0	40.0	33.0	40.0
27	37.6755088114503	40.0	37.0	40.0	33.0	40.0
28	37.62939858483019	40.0	37.0	40.0	33.0	40.0
29	37.644679697719766	40.0	37.0	40.0	33.0	40.0
30	37.608956058060215	40.0	37.0	40.0	33.0	40.0
31	37.56711366843178	40.0	37.0	40.0	33.0	40.0
32	37.50519684882599	40.0	36.0	40.0	33.0	40.0
33	37.42569694849418	40.0	36.0	40.0	33.0	40.0
34	37.32633356869722	40.0	36.0	40.0	33.0	40.0
35	37.218562981408624	40.0	35.0	40.0	33.0	40.0
36	37.12803416165778	40.0	35.0	40.0	33.0	40.0
37	36.973705611212516	39.0	35.0	40.0	32.0	40.0
38	36.74146711103747	39.0	35.0	40.0	32.0	40.0
39	36.64307884405756	39.0	35.0	40.0	32.0	40.0
40	36.47980362166476	39.0	35.0	40.0	31.0	40.0
41	36.290911659377024	38.0	35.0	40.0	31.0	40.0
42	36.12767033128	38.0	35.0	40.0	31.0	40.0
43	35.91903363807386	38.0	35.0	40.0	30.0	40.0
44	35.732634210152696	38.0	35.0	40.0	30.0	40.0
45	35.53698780208291	37.0	35.0	40.0	30.0	40.0
46	35.337421980901155	37.0	35.0	40.0	29.0	40.0
47	35.19298535031651	37.0	34.0	40.0	29.0	40.0
48	35.02267646899182	36.0	34.0	40.0	29.0	40.0
49	34.82001465276479	36.0	34.0	40.0	28.0	40.0
50	34.64149813735438	36.0	34.0	39.0	28.0	40.0
51	34.4260245143457	35.0	34.0	39.0	27.0	40.0
52	33.746208046031775	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	9.0
15	23.0
16	50.0
17	151.0
18	366.0
19	881.0
20	1689.0
21	3121.0
22	5179.0
23	8137.0
24	12253.0
25	17551.0
26	24955.0
27	33833.0
28	44798.0
29	59391.0
30	76339.0
31	95015.0
32	118594.0
33	152296.0
34	227964.0
35	374954.0
36	406014.0
37	778758.0
38	1391344.0
39	385332.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.906880518340962	21.156890532564717	35.01460891552712	14.921620033567203
2	25.11408511829465	20.563266310326217	37.027432336438096	17.295216234941037
3	24.30747198565347	21.116999553685602	40.324920674311606	14.25060778634932
4	24.791544155379036	17.998629532739873	40.60316202966628	16.60666428221481
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.59239146536892	0.24799721450514683	0.12363122152908783	0.03598009859684726
12	99.53206909980305	0.32962795203317186	0.09502253970669346	0.04328040845707714
13	97.89198812324913	1.2730270853346966	0.32576447636038786	0.5092203150557751
14	61.908855631395035	21.9057648508568	5.837522123138688	10.347857394609479
15	31.28900954942156	23.655729712190023	18.4713482984945	26.58391243989392
16	28.202850960618857	16.897870798262808	29.743666684917443	25.155611556200892
17	24.12802183645931	15.483459465148014	30.007662955122765	30.38085574326991
18	23.477820212804033	18.831362605205644	33.309844349334995	24.380972832655328
19	25.671515921193627	17.766536564715942	30.652365644078134	25.909581870012293
20	28.610506899859423	18.20912970114475	26.990098836240538	26.19026456275529
21	30.72878187456314	19.67826965590653	20.85117820601522	28.741770263515114
22	30.25125154094609	20.454567540783962	21.777440572988997	27.516740345280954
23	28.998324010031762	21.350846492260366	22.043475241402046	27.607354256305822
24	29.65876502933516	22.629562130732907	20.667722367319833	27.043950472612106
25	31.611953451517767	21.06682177454889	21.97900497250651	25.34221980142683
26	31.576352589796773	19.357601175065458	21.862958488494545	27.203087746643224
27	33.44418901260702	21.305338067157635	19.263005276844105	25.987467643391238
28	31.79540929021315	20.780189803315903	18.653832342695505	28.770568563775434
29	30.371066691411876	21.304176654225326	18.383128320248478	29.941628334114323
30	30.095977742587753	20.801758900630222	19.685498858852537	29.41676449792949
31	28.82046191525525	20.99941241986547	18.95267100087011	31.227454664009162
32	30.266847657465668	24.298702132899297	17.84259726062983	27.5918529490052
33	30.917926266396368	22.74800254752371	18.94127019228969	27.392800993790235
34	29.546534616386495	23.193321448997736	20.111547786572125	27.148596148043648
35	31.799035742838527	23.832359287120003	19.127356986811325	25.241247983230146
36	33.06454919757032	22.642313970683567	19.774216585498124	24.518920246247987
37	32.29633379860958	22.96061222105054	20.070874631636556	24.67217934870333
38	29.755920776468542	26.130890289379067	22.173387573687503	21.939801360464887
39	31.227454664009162	25.69723292183762	22.191780562166525	20.883531851986692
40	31.775404545011742	25.698323227855706	18.717425626315627	23.808846600816924
41	30.664264201058117	27.323993203127095	17.78466882784281	24.227073767971977
42	31.26146747131251	26.635654571143537	18.06959423313445	24.03328372440951
43	31.305980399616118	25.700148305320763	17.986920594197816	25.0069507008653
44	29.78955434689603	26.234232338049857	18.993273048891453	24.98294026616266
45	29.055565075981292	24.577630855091456	19.691993290351572	26.674810778575676
46	28.367416062435662	23.283058374747185	21.039706337925182	27.309819224891974
47	29.70598002037924	21.780308551862657	22.095478098003817	26.418233329754287
48	29.54786194545199	20.81557734429423	21.565091624814322	28.07146908543946
49	28.24297896254538	22.353169555148035	21.41825584694379	27.9855956353628
50	29.819348143955477	22.658052301031596	20.112495878761763	27.41010367625117
51	27.27447908852313	23.653880932420225	22.38578392647166	26.685856052584985
52	29.821955397476984	23.20991306231644	21.47030610815504	25.497825432051535
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	11.5
6	23.0
7	18.5
8	14.0
9	88.5
10	163.0
11	383.0
12	603.0
13	994.5
14	1747.0
15	2108.0
16	2567.0
17	3026.0
18	3784.0
19	4542.0
20	6462.5
21	8383.0
22	11889.5
23	15396.0
24	21835.5
25	28275.0
26	33220.5
27	38166.0
28	38784.5
29	39403.0
30	38393.0
31	37383.0
32	35704.5
33	34026.0
34	35794.0
35	37562.0
36	41929.0
37	46296.0
38	49433.0
39	59028.5
40	65487.0
41	72490.5
42	79494.0
43	93469.5
44	107445.0
45	121316.5
46	135188.0
47	158071.0
48	180954.0
49	199692.0
50	218430.0
51	246271.0
52	274112.0
53	290328.0
54	306544.0
55	330837.5
56	355131.0
57	378145.5
58	401160.0
59	404457.5
60	407755.0
61	386647.5
62	365540.0
63	321794.0
64	246208.5
65	214369.0
66	187435.0
67	160501.0
68	148856.5
69	137212.0
70	109075.0
71	80938.0
72	67288.0
73	53638.0
74	41253.5
75	28869.0
76	19282.0
77	9695.0
78	7752.0
79	5809.0
80	3901.5
81	1994.0
82	1564.0
83	1134.0
84	643.5
85	153.0
86	95.5
87	38.0
88	37.0
89	18.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	4218999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.004042967662901
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.68030149428206	7.471049449868178
2	8.722331764450463	1.7451716349954678
3	4.0063997622889	1.2024058610271775
4	2.379185951800299	0.9520591395948057
5	1.5251170209482254	0.7628668104140044
6	1.0322243511765519	0.6195850056862912
7	0.7195869512820309	0.5039145145716492
8	0.5384533879949789	0.43093686636683454
9	0.40763392353672084	0.3670188557524532
>10	2.85454634691294	5.676374934095896
>50	0.5852487099108691	4.300536301286053
>100	2.247165749369405	54.321616457652055
>500	0.27193477349247447	17.751300196869995
>1k	0.029869812554094294	3.895163971819146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.004693056338719208	0.0	0.0	0.0	0.0
12	0.014553215110977746	0.0	0.0	0.0	0.0
13	0.021616501923797566	0.0	0.0	0.0	0.0
14	0.07712729962723386	0.0	0.0	0.0	0.0
15	0.14197680539862656	0.0	0.0	0.0	0.0
16	0.16809674522321527	0.0	0.0	0.0	0.0
17	0.18701118440653813	0.0	0.0	0.0	0.0
18	0.21187490207985354	0.0	0.0	0.0	0.0
19	0.23403655701269424	0.0	0.0	0.0	0.0
20	0.26319039184413173	0.0	0.0	0.0	0.0
21	0.2978668636802237	0.0	0.0	0.0	0.0
22	0.33041012808962505	0.0	0.0	0.0	0.0
23	0.3621238118330912	0.0	0.0	0.0	0.0
24	0.39620772605065796	0.0	0.0	0.0	0.0
25	0.43349145140826056	0.0	0.0	0.0	0.0
26	0.4767718598653377	0.0	0.0	0.0	0.0
27	0.5202892913698249	0.0	0.0	0.0	0.0
28	0.5653236703777366	0.0	0.0	0.0	0.0
29	0.6241053861354317	0.0	0.0	0.0	0.0
30	0.6926761537511623	0.0	0.0	0.0	0.0
31	0.7505571819286992	0.0	0.0	0.0	0.0
32	0.7997868688757689	0.0	0.0	0.0	0.0
33	0.8370942965381125	0.0	0.0	0.0	0.0
34	0.87826519987324	0.0	0.0	0.0	0.0
35	0.9164259105062599	0.0	0.0	0.0	0.0
36	0.9562457824711501	0.0	0.0	0.0	0.0
37	0.9982462664722129	0.0	0.0	0.0	0.0
38	1.041337056491362	0.0	0.0	0.0	0.0
39	1.0885994521449283	0.0	0.0	0.0	0.0
40	1.1396068119475733	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTT	35	1.0204349E-7	46.000004	14
CGATAAT	70	0.0	46.000004	27
GCGTACT	35	1.0204349E-7	46.000004	13
TAACTAA	35	1.0204349E-7	46.000004	19
ATTAGGT	20	6.313381E-4	46.0	30
CGTATAA	30	1.8625942E-6	46.0	34
AATCACG	40	5.6170393E-9	46.0	14
CGACGTA	45	3.110472E-10	46.0	39
CGTCTAT	25	3.4187196E-5	46.0	22
CGATTAG	25	3.4187196E-5	46.0	36
ACGGGTA	20	6.313381E-4	46.0	34
ATGTACG	25	3.4187196E-5	46.0	27
ATATCGC	30	1.8625942E-6	46.0	26
CCGTAAC	55	1.8189894E-12	46.0	15
TACGCCC	45	3.110472E-10	46.0	40
TACGATA	50	1.6370905E-11	46.0	44
TACGACT	40	5.6170393E-9	46.0	32
CGTACTC	40	5.6170393E-9	46.0	21
CGTACTA	30	1.8625942E-6	46.0	17
CGTACGT	30	1.8625942E-6	46.0	42
>>END_MODULE