FastQCFastQC Report
Fri 17 Jun 2016
SRR1528016_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528016_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4184480
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCG206.3133735E-446.00000423
CGATTAG405.6170393E-946.00000436
ACTAGGT206.3133735E-446.00000428
CCGTACT206.3133735E-446.00000421
CGTACTC206.3133735E-446.00000421
TTACGTA206.3133735E-446.00000418
CACGTTA206.3133735E-446.00000439
ATCGGTT405.6170393E-946.00000414
CGACTTA405.6170393E-946.00000434
TTCGAAT206.3133735E-446.00000418
AATCACG253.4187135E-546.014
TTATCGA301.8625906E-646.038
ATATCGC253.4187135E-546.026
TACGCCC501.6370905E-1146.040
TACGCAC501.6370905E-1146.032
CGTACTA253.4187135E-546.017
TCGTACT253.4187135E-546.016
CGTAATC501.6370905E-1146.033
ACCGTTA2100.046.036
CGCGTAA301.8625906E-646.017
Produced by FastQC (version 0.11.5)