##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528016_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4184480
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.123239685695715	31.0	31.0	33.0	30.0	33.0
2	31.835720328451803	31.0	31.0	34.0	30.0	34.0
3	32.30828059878408	34.0	31.0	34.0	30.0	34.0
4	36.03382833709326	37.0	35.0	37.0	35.0	37.0
5	36.123002858180705	37.0	35.0	37.0	35.0	37.0
6	36.23764075823041	37.0	36.0	37.0	35.0	37.0
7	36.187364260314304	37.0	37.0	37.0	35.0	37.0
8	36.094845237640044	37.0	36.0	37.0	35.0	37.0
9	37.77928488127557	39.0	38.0	39.0	35.0	39.0
10	34.820531822353075	37.0	34.0	37.0	32.0	39.0
11	37.32064892172982	37.0	37.0	39.0	35.0	39.0
12	38.109899676901314	39.0	39.0	39.0	37.0	39.0
13	38.18953012082744	39.0	39.0	39.0	37.0	39.0
14	38.77294598134057	40.0	39.0	40.0	36.0	40.0
15	38.198998441861356	40.0	38.0	40.0	34.0	40.0
16	37.7298644037013	40.0	37.0	40.0	33.0	40.0
17	37.77371214583413	40.0	37.0	40.0	33.0	40.0
18	37.79234432952243	40.0	37.0	40.0	33.0	40.0
19	37.89051757924521	40.0	38.0	40.0	33.0	40.0
20	37.88285139372156	40.0	37.0	40.0	34.0	40.0
21	37.88111187053111	40.0	37.0	40.0	34.0	40.0
22	37.82812870416396	40.0	37.0	40.0	33.0	40.0
23	37.73093048598631	40.0	37.0	40.0	33.0	40.0
24	37.72826468282798	40.0	37.0	40.0	33.0	40.0
25	37.67006055710626	40.0	37.0	40.0	33.0	40.0
26	37.63417079302565	40.0	37.0	40.0	33.0	40.0
27	37.63915086223378	40.0	37.0	40.0	33.0	40.0
28	37.44937985125989	40.0	37.0	40.0	33.0	40.0
29	37.57264773639736	40.0	37.0	40.0	33.0	40.0
30	37.59697118877376	40.0	37.0	40.0	33.0	40.0
31	37.59293149925439	40.0	37.0	40.0	33.0	40.0
32	37.52864155163844	40.0	36.0	40.0	33.0	40.0
33	37.44444614384583	40.0	36.0	40.0	33.0	40.0
34	37.36864198179941	40.0	36.0	40.0	33.0	40.0
35	37.19459741712232	40.0	35.0	40.0	33.0	40.0
36	36.7271221274806	39.0	35.0	40.0	31.0	40.0
37	36.79718985393645	39.0	35.0	40.0	32.0	40.0
38	36.6792588326387	39.0	35.0	40.0	31.0	40.0
39	36.62017574465644	39.0	35.0	40.0	31.0	40.0
40	36.46503221427752	39.0	35.0	40.0	31.0	40.0
41	36.31066870913471	38.0	35.0	40.0	31.0	40.0
42	35.99916142507552	38.0	35.0	40.0	30.0	40.0
43	35.79166132948419	38.0	35.0	40.0	30.0	40.0
44	35.60910292318281	37.0	35.0	40.0	30.0	40.0
45	35.17823074790655	37.0	34.0	40.0	28.0	40.0
46	35.133355398998205	37.0	34.0	40.0	28.0	40.0
47	35.06854256681834	37.0	34.0	40.0	28.0	40.0
48	34.891995899132034	36.0	34.0	40.0	28.0	40.0
49	34.695632671204066	36.0	34.0	40.0	27.0	40.0
50	34.454293245516766	35.0	34.0	39.0	27.0	40.0
51	34.329907658777195	35.0	34.0	39.0	27.0	40.0
52	33.78671280541429	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	0.0
14	6.0
15	13.0
16	48.0
17	157.0
18	399.0
19	846.0
20	1656.0
21	3037.0
22	5287.0
23	8141.0
24	12362.0
25	17717.0
26	25219.0
27	34617.0
28	46234.0
29	60208.0
30	77414.0
31	96874.0
32	119996.0
33	154785.0
34	231211.0
35	373974.0
36	410142.0
37	776967.0
38	1340662.0
39	386505.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.92247065346232	21.11691297365503	34.99223320460368	14.968383168278972
2	25.25365158872787	20.618786563682942	36.86185619240622	17.265705655182963
3	24.381452414636946	21.048660765495356	40.30460176652774	14.26528505333996
4	24.83281076740718	17.962399151149008	40.619742476962486	16.58504760448132
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.58642411960386	0.2509750315451382	0.12577428975643332	0.03682655909455894
12	99.52419416510534	0.33311188009023823	0.09872194394524528	0.043972010859174855
13	97.89017990287921	1.2758813520437426	0.32333766680686726	0.5106010782701793
14	61.83893817152908	21.972096891370015	5.833795358085114	10.355169579015792
15	31.28340916912018	23.68038561541697	18.488055863572058	26.5481493518908
16	28.216982755324437	16.918231178067526	29.693056245937367	25.17172982067067
17	24.151364088249913	15.472006079608459	30.001744541735174	30.374885290406457
18	23.448337666806868	18.90454727947081	33.29816369058999	24.348951363132336
19	25.721834015218136	17.780369364891214	30.589941880472605	25.907854739418042
20	28.61112969831377	18.22811914503116	27.02569972852063	26.13505142813444
21	30.423684663327343	19.791587007226706	20.885964325316408	28.898764004129546
22	30.293345887661072	20.455349290712345	21.74466122433373	27.50664359729285
23	28.976838221236566	21.357325163461173	22.07753412610408	27.58830248919818
24	29.67195446029136	22.63817726455856	20.614891217068788	27.07497705808129
25	31.617309677666043	21.08577409857378	21.916558329828316	25.380357893931865
26	31.59957748633044	19.390366305968723	21.839009865025044	27.17104634267579
27	33.43199154972661	21.343344931747794	19.230083546820634	25.99457997170497
28	31.82101479753757	20.844143119336213	18.65338106526976	28.681461017856456
29	30.336146904752802	21.378068481627345	18.387278706075787	29.898505907544067
30	30.10926088785225	20.810160402248307	19.665358658662484	29.41522005123695
31	28.85402726264673	20.966547814782245	18.943070584636562	31.236354337934465
32	30.293298091997094	24.33626161434635	17.807756280350244	27.562684013306317
33	30.911558903376285	22.749087102818034	18.95286869575192	27.38648529805376
34	29.53963216457003	23.20610924176959	20.102545597063433	27.15171299659695
35	31.855355982105305	23.85151799028792	19.083733223721943	25.209392803884832
36	33.127318089702904	22.60223492524758	19.82267808664398	24.447768898405535
37	32.23071922915153	22.979509998852905	20.109260887852255	24.680509884143312
38	29.75284862157305	26.17053970863763	22.157567965434176	21.91904370435514
39	31.22947176232172	25.68921347455359	22.19664092073567	20.884673842389017
40	31.76349271594081	25.68481627346767	18.76866420678316	23.783026803808358
41	30.661563682942685	27.332237219439453	17.810886896340765	24.1953122012771
42	31.382441784881276	26.59219305624594	18.081912208924404	23.94345294994838
43	31.24617634688181	25.714067219821818	17.981947577715747	25.05780885558062
44	29.953447023286046	26.183540129239475	18.95251022827209	24.910502619202386
45	29.138865522119833	24.552417504683973	19.699221886590447	26.609495086605744
46	28.37466065078576	23.32753412610408	21.037930638932437	27.259874584177723
47	29.79330765112989	21.742247543302874	22.07610025618476	26.38834454938248
48	29.652692807708487	20.800099414981073	21.52341509578251	28.023792681527933
49	28.273907391121476	22.33426853515849	21.427608687339887	27.964215386380147
50	29.88827763545291	22.649504836921196	20.166759071617022	27.29545845600887
51	27.25308759989294	23.7088240354835	22.337829312124803	26.700259052498758
52	29.87764310021795	23.202142201659466	21.413269988146673	25.506944709975908
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	13.5
6	27.0
7	19.5
8	12.0
9	90.0
10	168.0
11	380.0
12	592.0
13	925.0
14	1677.5
15	2097.0
16	2574.5
17	3052.0
18	3790.0
19	4528.0
20	6299.0
21	8070.0
22	11613.5
23	15157.0
24	21290.5
25	27424.0
26	32362.0
27	37300.0
28	38257.5
29	39215.0
30	38227.0
31	37239.0
32	35632.5
33	34026.0
34	35689.5
35	37353.0
36	41753.5
37	46154.0
38	49171.5
39	58544.5
40	64900.0
41	72054.0
42	79208.0
43	92802.5
44	106397.0
45	120108.5
46	133820.0
47	157009.0
48	180198.0
49	198719.5
50	217241.0
51	244825.5
52	272410.0
53	287965.5
54	303521.0
55	328649.5
56	353778.0
57	375595.5
58	397413.0
59	400550.5
60	403688.0
61	383012.5
62	362337.0
63	319265.5
64	244122.5
65	212051.0
66	185641.0
67	159231.0
68	147277.0
69	135323.0
70	107749.0
71	80175.0
72	66595.0
73	53015.0
74	40816.0
75	28617.0
76	19305.0
77	9993.0
78	7909.5
79	5826.0
80	3898.0
81	1970.0
82	1560.5
83	1151.0
84	635.0
85	119.0
86	73.5
87	28.0
88	21.5
89	7.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	4184480.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.308152794832722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9970065493249	7.627724498706787
2	9.193375216713056	1.8953343286821314
3	4.206666598364197	1.3008888615857206
4	2.4229581880543036	0.9990489287181918
5	1.5732769856139102	0.8108789778151012
6	0.999666546950808	0.6182829305911048
7	0.7130175522646164	0.5144925711898904
8	0.5584864155806473	0.4605570644515007
9	0.41182883454400615	0.382067509661775
>10	2.787838814210073	5.741447600906071
>50	0.5959324903372665	4.50975135529858
>100	2.265716859176908	55.196556741983805
>500	0.24881496860860602	16.551938677283555
>1k	0.025413980256715592	3.3910299531257926
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.005520399189385539	0.0	0.0	0.0	0.0
12	0.01598764960042825	0.0	0.0	0.0	0.0
13	0.02382613849271594	0.0	0.0	0.0	0.0
14	0.07635357320383895	0.0	0.0	0.0	0.0
15	0.14324360494015984	0.0	0.0	0.0	0.0
16	0.17125186403089512	0.0	0.0	0.0	0.0
17	0.1896053989982029	0.0	0.0	0.0	0.0
18	0.21331204833097542	0.0	0.0	0.0	0.0
19	0.23701869766374795	0.0	0.0	0.0	0.0
20	0.2666520093297136	0.0	0.0	0.0	0.0
21	0.3007542155775628	0.0	0.0	0.0	0.0
22	0.33442626084961574	0.0	0.0	0.0	0.0
23	0.3665210492104156	0.0	0.0	0.0	0.0
24	0.4005515619622988	0.0	0.0	0.0	0.0
25	0.43587255764157073	0.0	0.0	0.0	0.0
26	0.47805223110159445	0.0	0.0	0.0	0.0
27	0.5197539479218445	0.0	0.0	0.0	0.0
28	0.5625071693495967	0.0	0.0	0.0	0.0
29	0.6184041983711238	0.0	0.0	0.0	0.0
30	0.6859394715711391	0.0	0.0	0.0	0.0
31	0.7464487821664818	0.0	0.0	0.0	0.0
32	0.7929300653844683	0.0	0.0	0.0	0.0
33	0.8325765686536918	0.0	0.0	0.0	0.0
34	0.8741348984820098	0.0	0.0	0.0	0.0
35	0.9127537949757198	0.0	0.0	0.0	0.0
36	0.9518745459411923	0.0	0.0	0.0	0.0
37	0.9909952969066647	0.0	0.0	0.0	0.0
38	1.0339874966543035	0.0	0.0	0.0	0.0
39	1.079034909952969	0.0	0.0	0.0	0.0
40	1.1288857874813596	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACCG	20	6.3133735E-4	46.000004	23
CGATTAG	40	5.6170393E-9	46.000004	36
ACTAGGT	20	6.3133735E-4	46.000004	28
CCGTACT	20	6.3133735E-4	46.000004	21
CGTACTC	20	6.3133735E-4	46.000004	21
TTACGTA	20	6.3133735E-4	46.000004	18
CACGTTA	20	6.3133735E-4	46.000004	39
ATCGGTT	40	5.6170393E-9	46.000004	14
CGACTTA	40	5.6170393E-9	46.000004	34
TTCGAAT	20	6.3133735E-4	46.000004	18
AATCACG	25	3.4187135E-5	46.0	14
TTATCGA	30	1.8625906E-6	46.0	38
ATATCGC	25	3.4187135E-5	46.0	26
TACGCCC	50	1.6370905E-11	46.0	40
TACGCAC	50	1.6370905E-11	46.0	32
CGTACTA	25	3.4187135E-5	46.0	17
TCGTACT	25	3.4187135E-5	46.0	16
CGTAATC	50	1.6370905E-11	46.0	33
ACCGTTA	210	0.0	46.0	36
CGCGTAA	30	1.8625906E-6	46.0	17
>>END_MODULE