FastQCFastQC Report
Fri 17 Jun 2016
SRR1528015_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528015_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4254837
Sequences flagged as poor quality0
Sequence length59
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA150.00669750453.018
CGACGTA650.053.039
TAGGTTA150.00669750453.053
TTCGTTA353.3449396E-853.048
TAACGTA203.13921E-453.020
CGATTAG203.13921E-453.036
CATTACG150.00669750453.016
TTATACG203.13921E-453.031
CTTATCC503.6379788E-1253.051
CGGATTA203.13921E-453.032
CGTACGT353.3449396E-853.042
TTACGTA203.13921E-453.018
ACCGTAA800.053.029
ACGTAAT401.6025297E-953.043
ATCGGTT307.0150054E-753.014
CACGTAA401.6025297E-953.018
TATACGT700.053.020
TCGACTA251.4794141E-553.053
TAATGCG251.4794141E-553.045
TACGTTC150.00669750453.031
Produced by FastQC (version 0.11.5)