##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1528015_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4254837 Sequences flagged as poor quality 0 Sequence length 59 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.881576662043692 31.0 31.0 33.0 30.0 33.0 2 31.55624034481227 31.0 31.0 34.0 30.0 34.0 3 32.03253685158797 33.0 31.0 34.0 30.0 34.0 4 35.86002354496776 37.0 35.0 37.0 35.0 37.0 5 35.888723116772745 37.0 35.0 37.0 35.0 37.0 6 36.02128236639852 37.0 35.0 37.0 35.0 37.0 7 35.95784186327232 37.0 35.0 37.0 35.0 37.0 8 35.866536837956424 37.0 35.0 37.0 35.0 37.0 9 37.591110070726565 39.0 37.0 39.0 35.0 39.0 10 34.22414936224349 35.0 33.0 37.0 28.0 39.0 11 36.78922412303926 37.0 35.0 39.0 34.0 39.0 12 37.7799111928377 39.0 37.0 39.0 35.0 39.0 13 37.93369311209807 39.0 38.0 39.0 35.0 39.0 14 38.252069585744415 40.0 38.0 40.0 35.0 40.0 15 37.65746936956692 40.0 37.0 40.0 33.0 40.0 16 37.18668517736402 39.0 36.0 40.0 32.0 40.0 17 37.1507244578347 39.0 36.0 40.0 32.0 40.0 18 37.10146546154412 39.0 36.0 40.0 32.0 40.0 19 37.20426469921174 40.0 36.0 40.0 32.0 40.0 20 37.29753031667253 40.0 36.0 40.0 32.0 40.0 21 37.25594540989467 40.0 36.0 40.0 32.0 40.0 22 37.22300313737048 40.0 36.0 40.0 32.0 40.0 23 37.151206732478826 39.0 36.0 40.0 32.0 40.0 24 37.11452377611645 39.0 36.0 40.0 32.0 40.0 25 37.04519985136916 39.0 36.0 40.0 31.0 40.0 26 36.89742027720451 39.0 36.0 40.0 31.0 40.0 27 36.76870418302746 39.0 35.0 40.0 31.0 40.0 28 36.73189431228506 39.0 35.0 40.0 31.0 40.0 29 36.71261954335736 39.0 35.0 40.0 31.0 40.0 30 36.68887973851877 39.0 35.0 40.0 31.0 40.0 31 36.68220944774148 39.0 35.0 40.0 31.0 40.0 32 36.547010143984366 39.0 35.0 40.0 30.0 40.0 33 36.396973374068146 39.0 35.0 40.0 30.0 40.0 34 36.299658952857655 39.0 35.0 40.0 30.0 40.0 35 36.13135873360131 38.0 35.0 40.0 30.0 40.0 36 35.94468037200955 38.0 35.0 40.0 30.0 40.0 37 35.77929283777498 38.0 35.0 40.0 29.0 40.0 38 35.52729187980644 38.0 34.0 40.0 28.0 40.0 39 35.41207736042532 38.0 34.0 40.0 28.0 40.0 40 35.20897016736481 38.0 34.0 40.0 27.0 40.0 41 35.02862365820359 37.0 34.0 40.0 27.0 40.0 42 34.897107926813646 37.0 34.0 40.0 27.0 40.0 43 34.63529930758805 37.0 33.0 40.0 26.0 40.0 44 34.451890871495195 36.0 33.0 40.0 26.0 40.0 45 34.19856788873463 36.0 33.0 40.0 24.0 40.0 46 33.938794130068906 36.0 33.0 39.0 24.0 40.0 47 33.83625248158743 35.0 33.0 39.0 23.0 40.0 48 33.6273114575247 35.0 33.0 39.0 23.0 40.0 49 33.454447491172985 35.0 33.0 39.0 23.0 40.0 50 33.23572089835639 35.0 32.0 39.0 23.0 40.0 51 33.02272495985157 35.0 32.0 38.0 23.0 40.0 52 32.7856221049126 35.0 32.0 38.0 22.0 40.0 53 32.47772241333804 35.0 31.0 38.0 21.0 40.0 54 32.21871766180467 35.0 31.0 37.0 20.0 40.0 55 32.050543886875104 35.0 31.0 37.0 20.0 40.0 56 31.79558723401155 35.0 31.0 37.0 20.0 39.0 57 31.594806804584994 34.0 31.0 36.0 18.0 39.0 58 31.351608298978316 34.0 30.0 36.0 18.0 39.0 59 30.770661954852795 34.0 29.0 35.0 17.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 3.0 14 17.0 15 90.0 16 309.0 17 883.0 18 2129.0 19 4213.0 20 7398.0 21 11497.0 22 16689.0 23 23642.0 24 32292.0 25 42819.0 26 54710.0 27 69295.0 28 85196.0 29 100258.0 30 121243.0 31 147303.0 32 181595.0 33 230564.0 34 322592.0 35 421387.0 36 539742.0 37 805324.0 38 917453.0 39 116192.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.070067784030268 21.437883519392166 34.480427804872434 15.011620891705135 2 24.882527814814058 21.03203953523954 36.75623766550869 17.329194984437713 3 24.27836836052709 21.254656758884067 40.16052788861242 14.306446991976426 4 24.904549810016224 18.136675976071466 40.336821363544594 16.62195285036771 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 0.0 100.0 0.0 0.0 8 0.0 100.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 0.0 0.0 100.0 0.0 11 99.5729801165121 0.2581297473910281 0.1267263587300759 0.042163777366794546 12 99.5181484038049 0.34109414767240204 0.09236546546906498 0.04839198305363989 13 97.85176259396071 1.3129997694388762 0.32356116109735816 0.5116764755030568 14 61.51032342719592 22.214552519873266 5.914468638869127 10.36065541406169 15 31.30773282266747 23.880162741839463 18.41511202426791 26.396992411225156 16 28.35159137706098 16.975456404087865 29.55478670510762 25.118165513743534 17 24.221280392174833 15.559091922910326 29.835714035578803 30.383913649336037 18 23.425715250666475 19.035112273396138 33.13842574932953 24.400746726607856 19 25.760493292692527 17.913189153897083 30.590784088791178 25.73553346461921 20 28.706105545288807 18.32500281444389 26.999506679104275 25.969384961163023 21 30.494940229202673 19.93864864858513 20.87287950161193 28.69353162060027 22 30.23406067024424 20.565018119378017 21.810753267398965 27.390167942978778 23 28.943811478559578 21.55824065645758 22.165855002201024 27.332092862781813 24 29.665531253018624 22.85380144997329 20.62212959039324 26.85853770661485 25 31.530021009030428 21.28011014287974 21.98387388283029 25.20599496525954 26 31.506941393994648 19.49677508210068 21.931862489679393 27.064421034225283 27 33.3146487162728 21.480423339366467 19.32085764977601 25.884070294584728 28 31.662105974917488 20.989029662005855 18.63114850228105 28.717715860795607 29 30.260477663421653 21.569662950660625 18.35696643608204 29.81289294983568 30 30.02467544585139 20.96935793310061 19.714198217229004 29.291768403818995 31 28.688549056050793 21.197404271891028 18.9463897206873 31.167656951370876 32 30.10350337745018 24.679347293445083 17.82077668310208 27.396372646002654 33 30.87133537665485 23.059684777583726 18.87552919183508 27.193450653926345 34 29.519203673372214 23.44353026919715 20.078771525207664 26.95849453222297 35 31.75296726995652 24.079206794525852 19.0821881073235 25.085637828194123 36 32.88565460909548 22.925155534747866 19.88198372816632 24.307206127990334 37 32.19352468731469 23.237247396316242 20.08734999719143 24.481877919177634 38 29.58679733207171 26.457394255056073 22.16691262203464 21.788895790837582 39 30.959775897408054 26.02696648543763 22.212319766891188 20.800937850263125 40 31.535426621513352 26.134444163195912 18.66715458194991 23.66297463334083 41 30.469580855858876 27.760029350125514 17.75257195516538 24.01781783885023 42 31.07282370629004 27.04216401239342 17.950417372040338 23.934594909276196 43 31.144342309705404 26.07759122147335 17.849144397305935 24.928922071515313 44 29.592085431239784 26.56856185090052 18.933228229424532 24.906124488435164 45 28.89546650083188 24.834535377030896 19.717371076729847 26.55262704540738 46 28.283950713035537 23.58332410853812 20.94898112430629 27.18374405412005 47 29.612415234708166 22.040750327215825 21.997011871430093 26.349822566645916 48 29.41097391039892 21.10992736031956 21.43438162260975 28.044717106671772 49 28.083990056493352 22.714524669217646 21.28135578401711 27.920129490271894 50 29.64856233035484 22.992749193447366 20.026149062819563 27.332539413378232 51 27.084280784434277 24.02122572498077 22.33300594123817 26.56148754934678 52 29.708447115600435 23.497586394026378 21.37870381403565 25.41526267633754 53 28.73527235003362 23.640882130149755 21.88142577494743 25.742419744869196 54 26.458428372226717 23.498150457937637 23.08323444587889 26.96018672395676 55 26.98112759666234 23.91934168100917 22.3467314964122 26.75279922591629 56 27.662140758858683 24.349064370738528 22.22256692794577 25.76622794245702 57 28.799293603961797 23.33038844966329 21.29371818473892 26.576599761635993 58 28.682250342375042 22.737157733657014 21.631780488888293 26.94881143507965 59 28.041708765811713 23.698111114479826 21.031969027250632 27.228211092457833 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 5.0 5 9.0 6 10.0 7 11.0 8 60.0 9 265.5 10 422.0 11 661.5 12 901.0 13 1128.5 14 1469.0 15 1582.0 16 1743.5 17 1905.0 18 2292.5 19 3518.5 20 4357.0 21 6642.5 22 8928.0 23 12466.0 24 16004.0 25 20160.0 26 27271.5 27 30227.0 28 33251.5 29 36276.0 30 37002.5 31 38509.0 32 39289.0 33 41027.5 34 42766.0 35 43782.0 36 49139.5 37 53481.0 38 54429.0 39 55377.0 40 62027.0 41 68677.0 42 72141.5 43 86391.0 44 97176.0 45 107318.5 46 117461.0 47 128677.5 48 156153.5 49 172413.0 50 184247.0 51 196081.0 52 215770.5 53 256634.0 54 277808.0 55 311271.5 56 344735.0 57 360824.5 58 371884.0 59 366854.0 60 340241.0 61 313628.0 62 295453.0 63 277278.0 64 240548.5 65 185264.5 66 166710.0 67 149826.5 68 132943.0 69 121989.5 70 96308.5 71 81581.0 72 64864.0 73 48147.0 74 35948.0 75 18009.5 76 12270.0 77 9158.5 78 6047.0 79 5146.5 80 4246.0 81 2711.5 82 748.0 83 319.0 84 251.5 85 184.0 86 131.5 87 49.0 88 19.0 89 10.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 59 4254837.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.771201571024447 #Duplication Level Percentage of deduplicated Percentage of total 1 81.69919083355391 12.067952159920177 2 7.035782366074813 2.0785391907830073 3 3.0077083110163416 1.3328239718660362 4 1.698778903537009 1.0037202243499623 5 1.0691690613155176 0.7896455859097253 6 0.667154875950537 0.5912807491054315 7 0.4552235983233268 0.4706939671504649 8 0.32649063209518236 0.3858127150183298 9 0.251630620848747 0.33451979597989817 >10 1.718911237992201 5.113825867447408 >50 0.3901082631105318 4.253068483329533 >100 1.4961353896313456 52.27434411446179 >500 0.16589513960134925 15.8901049765588 >1k 0.017820766949363687 3.4136681981194865 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.005546628460737744 0.0 0.0 0.0 0.0 12 0.014971196311398063 0.0 0.0 0.0 0.0 13 0.022915096394997034 0.0 0.0 0.0 0.0 14 0.07403338835306734 0.0 0.0 0.0 0.0 15 0.13730255706622838 0.0 0.0 0.0 0.0 16 0.1631789889953481 0.0 0.0 0.0 0.0 17 0.18040644095179204 0.0 0.0 0.0 0.0 18 0.202592954794743 0.0 0.0 0.0 0.0 19 0.22381585945595567 0.0 0.0 0.0 0.0 20 0.2497863020369523 0.0 0.0 0.0 0.0 21 0.281749923675102 0.0 0.0 0.0 0.0 22 0.3106581991272521 0.0 0.0 0.0 0.0 23 0.34017754381660215 0.0 0.0 0.0 0.0 24 0.3711775562730135 0.0 0.0 0.0 0.0 25 0.404668851004163 0.0 0.0 0.0 0.0 26 0.44450586473700404 0.0 0.0 0.0 0.0 27 0.4843193758068758 0.0 0.0 0.0 0.0 28 0.5234043043247015 0.0 0.0 0.0 0.0 29 0.5781185037170636 0.0 0.0 0.0 0.0 30 0.6428448375343169 0.0 0.0 0.0 0.0 31 0.6975120316007405 0.0 0.0 0.0 0.0 32 0.7430366897721347 0.0 0.0 0.0 0.0 33 0.778572716181607 0.0 0.0 0.0 0.0 34 0.8149078331320331 0.0 0.0 0.0 0.0 35 0.85030284356369 0.0 0.0 0.0 0.0 36 0.8861444045917622 0.0 0.0 0.0 0.0 37 0.9236781573536189 0.0 0.0 0.0 0.0 38 0.9637737003791215 0.0 0.0 0.0 0.0 39 1.0054909271495007 0.0 0.0 0.0 0.0 40 1.0518851838507561 0.0 0.0 0.0 0.0 41 1.0992195470707808 0.0 0.0 0.0 0.0 42 1.1463423863240825 0.0 0.0 0.0 0.0 43 1.1914439965620305 0.0 0.0 0.0 0.0 44 1.2325501540952097 0.0 0.0 0.0 0.0 45 1.270553960116451 0.0 0.0 0.0 0.0 46 1.309051322060046 0.0 0.0 0.0 0.0 47 1.3439997818952876 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCATA 15 0.006697504 53.0 18 CGACGTA 65 0.0 53.0 39 TAGGTTA 15 0.006697504 53.0 53 TTCGTTA 35 3.3449396E-8 53.0 48 TAACGTA 20 3.13921E-4 53.0 20 CGATTAG 20 3.13921E-4 53.0 36 CATTACG 15 0.006697504 53.0 16 TTATACG 20 3.13921E-4 53.0 31 CTTATCC 50 3.6379788E-12 53.0 51 CGGATTA 20 3.13921E-4 53.0 32 CGTACGT 35 3.3449396E-8 53.0 42 TTACGTA 20 3.13921E-4 53.0 18 ACCGTAA 80 0.0 53.0 29 ACGTAAT 40 1.6025297E-9 53.0 43 ATCGGTT 30 7.0150054E-7 53.0 14 CACGTAA 40 1.6025297E-9 53.0 18 TATACGT 70 0.0 53.0 20 TCGACTA 25 1.4794141E-5 53.0 53 TAATGCG 25 1.4794141E-5 53.0 45 TACGTTC 15 0.006697504 53.0 31 >>END_MODULE