FastQCFastQC Report
Fri 17 Jun 2016
SRR1528014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339394
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGTCGGTGCCCGTTACGGCCTTTCCGTCGGCCACCTTACAGTCCTTGCA101132.9797226821923783No Hit
TTAGGTCGGTGGGGGGATGGAGTTGCTGAATGGTAACAGTAATTCAAACCCA10540.31055351597258646No Hit
AGGCGTCGGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGC9940.2928749477009022No Hit
AATGGTCGGTGGGTAACAGCGTGGTTTGCTGATGAATTCCCATTTTCGCCAG9380.27637495064733025No Hit
GTTAGTCGGTGGGGAAGGGCATCGAGCACACCCAGCACAGCAGGAAGGACCA8040.23689281484056876No Hit
GGTTGTCGGTGGGGACTCCTCTACACTCTCTGCACAACGTCTAAATTATGTG7930.23365174399076002No Hit
CCATGTCGGTGGGGCGTGCGCAGCCTCGCTATTTAATTTTCTTCCTTAGAGA7760.2286428163137828No Hit
CCAGGTCGGTGGGTTTCCGTCGGTGGGGCTCTTTCCTTTTGCGGCCATCGGT7440.21921424656888452No Hit
CGTAGTCGGTGGGACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAA7270.21420531889190733No Hit
GTGCGTCGGTGGGGAGTGAGTGTCGGTGGGAGAGAGAGAGAGAGAGAGAGAA7270.21420531889190733No Hit
ATTCGTCGGTGGGATTGCCCAGCGCCATCGCGCCAAGATCGACAACTACATC7120.20978567682398627No Hit
TATAGTCGGTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCC6960.20507139195153715No Hit
GTAGGTCGGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTA6700.1974106790338073No Hit
GCTTGTCGGTGGGGACAAATCTACAGCTAAGATCATACTGAGTGTGGAAAAG6480.19092853733418977No Hit
GAGCGTCGGTGGTCCTAATAATTTAGAGTGCGGGGCAAAAGAATTGCTGTTT6350.18709818087532484No Hit
CTTGGTCGGTGGGTGCTGGGAAAACTGGCTAGCCTTATGTAGAAAGCTGAAA6220.18326782441645992No Hit
CTTGGTCGGTGGGGCTCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCA6130.1806160391757073No Hit
CTAGGTCGGTGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATG6040.17796425393495466No Hit
TTGGGTCGGTGGGGCTCCTGCTGCTCCTGCAGGTCCTAAGCAGCGCCGCCAC5810.17118746943080904No Hit
GGATGTCGGTGGGTACACCCACTAGAATGGCGATAAAAAAGACAGTAACAAG5550.16352675651307919No Hit
ATGCGTCGGTGGGGGGGTGACTGGAAGTTTGTGGATATCAGGGTATACACTA5500.16205354249043882No Hit
TTACGTCGGTGGGGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTT5490.16175889968591078No Hit
GTTGGTCGGTGGGGCAATCCCCAGCTTTCCTTCGCAACTCGAATGGAAGATT5420.15969640005421426No Hit
TCTTGTCGGTGGGGGTCCCCACTCTGGATCCCCTTTGCCACTCCTGGGGAGC5390.15881247164063006No Hit
ACGCGTCGGTGGGGCCGTCGGTGGGGTAGTCGGTGGGCCTTTCCGGCGGTGA5250.15468747237723707No Hit
GCTTGTCGGTGGGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTT5240.154392829572709No Hit
TTTAGTCGGTGGGGGTGAAACCCTGTCTATACTAAAATACAAAAAAATTAGC5210.15350890115912477No Hit
TCTCGTCGGTGGGGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATC5130.15115175872290024No Hit
TCGTGTCGGTGGGATATCTTATTGACCCAGATATATTTTGATCAACGGACCA5080.14967854470025987No Hit
TCTGGTCGGTGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATG4900.14437497421875461No Hit
TAAAGTCGGTGGGGAGTTCGAATCTCTTCACTCCGACCATACACTGTCCCGG4880.14378568860969845No Hit
TTTTGTCGGTGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCA4880.14378568860969845No Hit
GATTGTCGGTGGGGCTTCCTGCCTCCTGTGCCCACCGTTCTTAGGCATCAGC4760.14024997495536162No Hit
TAAGGTCGGTGGGTGCTCTCACTTTGGAAGAGCTGTGCAGTTGCTTTTGGCA4730.13936604654177742No Hit
ATAAGTCGGTGGGGCACGGGGCCATGTCCTGGGCGCCGAGCGTGGCGGGCTG4710.13877676093272126No Hit
CGAGGTCGGTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATG4700.1384821181281932No Hit
GTTAGTCGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG4620.13612497569196863No Hit
GTGGGTCGGTGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATG4570.13465176166932827No Hit
CTAAGTCGGTGGGGTTCGGCATTCAGTCCGGGGCTGCAGCAGAAAAGCCATT4340.12787497716518265No Hit
CACGGTCGGTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGG4290.12640176314254228No Hit
GTAGGTCGGTGGGGTGGATGGTGGATGTGTGGATGGATGAGTGGGTGGATAG4260.12551783472895808No Hit
TTGTGTCGGTAGGCTCCACTGAAACCTTCCAATAACCTGCCATCTGCCAAGG4250.12522319192443002No Hit
AGACGTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT4210.12404462070631772No Hit
TTTAGTCGGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC4200.12374997790178965No Hit
ATGCGTCGGTGGGATGTCAGCAATATGAACAAGATGGCTGGCATGCATGAAA4190.1234553350972616No Hit
TGTTGTCGGTGGGCTTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCT4180.12316069229273352No Hit
CGCGGTCGGTGGGATTCCAGCTCCAGCCACTGCTGCTCGTTGCCGTTGATTA4160.12257140668367737No Hit
CTGGGTCGGTGGGGAGGAGGCTGGGGCCCTGGAGACGATGCGCCCCGCGGAG4140.12198212107462124No Hit
TCCTGTCGGTGGGGCTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTA4130.12168747827009316No Hit
TCTGGTCGGTGGGCCCTCTGAAGTTGGGGGAAAGGAGCTGGGGATGGGTATA4100.12080354985650894No Hit
ATTCGTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT3990.11756247900670018No Hit
AGTGGTCGGTGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCG3970.11697319339764403No Hit
AGGTGTCGGTGGGTGAAATTGAATTCCCCGTGAGGATGCGGGGTTCCTGCGG3900.11491069376594755No Hit
TTAAGTCGGTGGTTAATGAGAACATAGTTGAGAAGACTCATGATATCAATAA3870.11402676535236334No Hit
GTATGTCGGTGGGGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCCG3820.11255355132972297No Hit
CGGAGTCGGTGGGGAGTCGGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCT3810.11225890852519489No Hit
CCCGGTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT3810.11225890852519489No Hit
TGACGTCGGTGGGAGGTTTGGGTCTGTGGAAGAGTGTGTTCAGGATGGTAAC3760.11078569450255456No Hit
GTAAGTCGGTGGGGCTCTAGGGGAACTGCACTGAGGGACTTCCACATGTCTC3740.11019640889349841No Hit
TTTCGTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT3720.10960712328444226No Hit
AAGCGTCGGTGGCACTTTAGCTGTCTTCAGACACATCAAAAGAAGGTTTCAG3720.10960712328444226No Hit
ATTGGTCGGTGGCTTTTTTTCCTACTTGGGCCCGGCAGAATGGCTCCCGCAA3690.10872319487085806No Hit
CGGCGTCGGTGGGGCTTCACTCTCAGGTCAAAATGGCGGGCCGATCTGCTGT3670.10813390926180191No Hit
TCTGGTCGGTGGGGGGCTGTTGAGATGGCTCAGTGGGTAAGAGCACTGACTG3660.10783926645727385No Hit
TTCTGTCGGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGC3620.10666069523916155No Hit
AGGGGTCGGTGGGGCCAGGCACAGTAGCTCATGGTGTAACCCCAGCACTTGG3610.1063660524346335No Hit
TTCTGTCGGTGGGAGGCGCGCCGAGGACTCGCTGGAGCGGGCAGGTGGAGGG3610.1063660524346335No Hit
ACAAGTCGGTGGGCAACGCAGAGTTTTGGTCGGTGGGAAGCAGTGGTATCAA3610.1063660524346335No Hit
CAGCGTCGGTGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGG3600.10607140963010543No Hit
TGTGGTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGA3590.10577676682557735No Hit
GTTGGTCGGTGGACTTGGGAACTGCGTTTGAAACTCCAAAGCTAGAGTATAG3560.10489283841199314No Hit
AGGAGTCGGTGGGATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGT3510.10341962438935277No Hit
CGGCGTCGGTGGGACATTCTCTCTCGTAGGGCCAGTTGTTATCACGGTATGG3510.10341962438935277No Hit
GGGCGTCGGTGGGTATGGGGGACCTTTGGGATAGCATTGAAAATGTAAATGA3500.10312498158482472No Hit
TTTTGTCGGTGGGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAG3470.1022410531712405No Hit
GGGGGTCGGTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC3460.10194641036671243No Hit
CTTGGTCGGTGGGGGTGGTGCACACCTGTAGTCCCAGCTACTCGGGAGGCTT3450.10165176756218436No Hit
ATAAGTCGGTGGGAAACTGGTATCTCAGAAACTAAGCTGAGCACAGTGGCTC3430.10106248195312821No Hit
CCTCGTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT3410.10047319634407209No Hit
GGCAGTCGGTGGGTCCTGGCGATGAGGAGCAGGAAGAAGAAACTCAAGAGCA3410.10047319634407209No Hit
CTATGTCGGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG3400.10017855353954401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCC405.5915734E-946.00000426
TTGATGA206.3051365E-446.00000445
ACAACGT800.046.00000434
CTTAACT405.5915734E-946.00000430
GTCATAA750.046.00000429
TTTAGAG750.046.00000422
TATCAGA206.3051365E-446.00000423
AACGTGG206.3051365E-446.00000416
AGGTATG206.3051365E-446.00000421
CTAGAAG206.3051365E-446.00000437
CTAGAAA405.5915734E-946.00000435
AGCGTCA405.5915734E-946.00000439
AGCGTAG206.3051365E-446.00000441
AGGTAGC206.3051365E-446.00000422
ATCTCTT405.5915734E-946.00000422
TCCATTT800.046.00000433
GTCGCTA206.3051365E-446.00000419
CCTTACC206.3051365E-446.00000419
CTGTCCG206.3051365E-446.00000444
TTGGCCC206.3051365E-446.00000420
Produced by FastQC (version 0.11.5)