FastQCFastQC Report
Fri 17 Jun 2016
SRR1528013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337382
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGTCGGTGCCCGTTACGGCCTTTCCGTCGGCCACCTTACAGTCCTTGCA96912.8724116876419012No Hit
TTAGGTCGGTGGGGGGATGGAGTTGCTGAATGGTAACAGTAATTCAAACCCA9410.27891233082974193No Hit
AGGCGTCGGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGC9060.26853833340249333No Hit
AATGGTCGGTGGGTAACAGCGTGGTTTGCTGATGAATTCCCATTTTCGCCAG9020.267352733696522No Hit
GTTAGTCGGTGGGGAAGGGCATCGAGCACACCCAGCACAGCAGGAAGGACCA7810.23148834259089104No Hit
GTGCGTCGGTGGGGAGTGAGTGTCGGTGGGAGAGAGAGAGAGAGAGAGAGAA7660.22704234369349874No Hit
CGTAGTCGGTGGGACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAA7540.22348554457558492No Hit
CCAGGTCGGTGGGTTTCCGTCGGTGGGGCTCTTTCCTTTTGCGGCCATCGGT7190.2131115471483363No Hit
CCATGTCGGTGGGGCGTGCGCAGCCTCGCTATTTAATTTTCTTCCTTAGAGA7050.20896194817743688No Hit
GGTTGTCGGTGGGGACTCCTCTACACTCTCTGCACAACGTCTAAATTATGTG7020.2080727483979584No Hit
TATAGTCGGTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCC6810.2018483499416092No Hit
ATTCGTCGGTGGGATTGCCCAGCGCCATCGCGCCAAGATCGACAACTACATC6510.19295635214682466No Hit
GCTTGTCGGTGGGGACAAATCTACAGCTAAGATCATACTGAGTGTGGAAAAG6320.18732475354346112No Hit
GAGCGTCGGTGGTCCTAATAATTTAGAGTGCGGGGCAAAAGAATTGCTGTTT6240.1849535541315186No Hit
GTAGGTCGGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTA6140.18198955486659038No Hit
CTTGGTCGGTGGGTGCTGGGAAAACTGGCTAGCCTTATGTAGAAAGCTGAAA5790.17161555743934176No Hit
CTTGGTCGGTGGGGCTCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCA5720.16954075795389204No Hit
TCTTGTCGGTGGGGGTCCCCACTCTGGATCCCCTTTGCCACTCCTGGGGAGC5460.16183435986507874No Hit
CTAGGTCGGTGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATG5410.16035236023261465No Hit
GTTGGTCGGTGGGGCAATCCCCAGCTTTCCTTCGCAACTCGAATGGAAGATT5300.15709196104119366No Hit
TTACGTCGGTGGGGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTT5290.15679556111470083No Hit
TTGGGTCGGTGGGGCTCCTGCTGCTCCTGCAGGTCCTAAGCAGCGCCGCCAC5200.15412796177626548No Hit
ACGCGTCGGTGGGGCCGTCGGTGGGGTAGTCGGTGGGCCTTTCCGGCGGTGA5190.15383156184977265No Hit
ATGCGTCGGTGGGGGGGTGACTGGAAGTTTGTGGATATCAGGGTATACACTA5120.15175676236432292No Hit
GGATGTCGGTGGGTACACCCACTAGAATGGCGATAAAAAAGACAGTAACAAG5020.14879276309939474No Hit
GTTAGTCGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG5000.14819996324640913No Hit
GCTTGTCGGTGGGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTT4940.1464215636874522No Hit
ATAAGTCGGTGGGGCACGGGGCCATGTCCTGGGCGCCGAGCGTGGCGGGCTG4840.14345756442252403No Hit
TAAAGTCGGTGGGGAGTTCGAATCTCTTCACTCCGACCATACACTGTCCCGG4770.1413827649370743No Hit
TCTCGTCGGTGGGGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATC4760.14108636501058147No Hit
TTTAGTCGGTGGGGGTGAAACCCTGTCTATACTAAAATACAAAAAAATTAGC4750.14078996508408867No Hit
TCGTGTCGGTGGGATATCTTATTGACCCAGATATATTTTGATCAACGGACCA4660.1381223657456533No Hit
TTGTGTCGGTAGGCTCCACTGAAACCTTCCAATAACCTGCCATCTGCCAAGG4610.1366403661131892No Hit
CGAGGTCGGTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATG4400.13041596765684002No Hit
TAAGGTCGGTGGGTGCTCTCACTTTGGAAGAGCTGTGCAGTTGCTTTTGGCA4400.13041596765684002No Hit
TCTGGTCGGTGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATG4380.1298231678038544No Hit
CTAAGTCGGTGGGGTTCGGCATTCAGTCCGGGGCTGCAGCAGAAAAGCCATT4380.1298231678038544No Hit
GTGGGTCGGTGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATG4340.1286375680978831No Hit
TTTTGTCGGTGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCA4330.1283411681713903No Hit
GATTGTCGGTGGGGCTTCCTGCCTCCTGTGCCCACCGTTCTTAGGCATCAGC4280.1268591685389262No Hit
TCCTGTCGGTGGGGCTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTA4280.1268591685389262No Hit
CACGGTCGGTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGG4200.12448796912698365No Hit
ATGCGTCGGTGGGATGTCAGCAATATGAACAAGATGGCTGGCATGCATGAAA4190.12419156920049083No Hit
TCTGGTCGGTGGGCCCTCTGAAGTTGGGGGAAAGGAGCTGGGGATGGGTATA4010.11885637052362011No Hit
GTAAGTCGGTGGGGCTCTAGGGGAACTGCACTGAGGGACTTCCACATGTCTC4000.11855997059712728No Hit
CCCGGTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT3930.11648517111167757No Hit
TCTGGTCGGTGGGGGGCTGTTGAGATGGCTCAGTGGGTAAGAGCACTGACTG3890.1152995714057063No Hit
TTTAGTCGGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC3880.11500317147921348No Hit
TTCTGTCGGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGC3860.11441037162622783No Hit
GTAGGTCGGTGGGGTGGATGGTGGATGTGTGGATGGATGAGTGGGTGGATAG3840.1138175717732422No Hit
CGCGGTCGGTGGGATTCCAGCTCCAGCCACTGCTGCTCGTTGCCGTTGATTA3830.11352117184674937No Hit
ATTCGTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT3740.11085357250831401No Hit
CTGGGTCGGTGGGGAGGAGGCTGGGGCCCTGGAGACGATGCGCCCCGCGGAG3740.11085357250831401No Hit
CGGAGTCGGTGGGGAGTCGGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCT3730.1105571725818212No Hit
TTAAGTCGGTGGTTAATGAGAACATAGTTGAGAAGACTCATGATATCAATAA3710.10996437272883557No Hit
AGGTGTCGGTGGGTGAAATTGAATTCCCCGTGAGGATGCGGGGTTCCTGCGG3690.10937157287584992No Hit
AGTGGTCGGTGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCG3640.10788957324338584No Hit
CTTGGTCGGTGGGGGTGGTGCACACCTGTAGTCCCAGCTACTCGGGAGGCTT3610.1070003734639074No Hit
ACAAGTCGGTGGGCAACGCAGAGTTTTGGTCGGTGGGAAGCAGTGGTATCAA3600.10670397353741455No Hit
TGTTGTCGGTGGGCTTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCT3560.10551837383144329No Hit
AGACGTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACT3530.10462917405196484No Hit
CGGCGTCGGTGGGACATTCTCTCTCGTAGGGCCAGTTGTTATCACGGTATGG3510.1040363741989792No Hit
AGGAGTCGGTGGGATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGT3470.10285077449300793No Hit
TTCTGTCGGTGGGAGGCGCGCCGAGGACTCGCTGGAGCGGGCAGGTGGAGGG3460.1025543745665151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCAA750.046.00000439
GTCGCAT1050.046.00000445
AGATATA750.046.00000429
ATCAGCG1050.046.00000433
TCTTATT750.046.00000417
TGGCGCT1050.046.00000416
GCACGAT750.046.00000445
CACGATG750.046.00000446
GGATACC750.046.00000412
TGCGGAG750.046.00000434
CCGTCAG750.046.00000434
GCCGCAC750.046.00000442
TATTGAC750.046.00000420
AACGGAC750.046.00000444
TTCCCCT750.046.00000419
CTTATTG750.046.00000418
TATCTTA750.046.00000415
TCAGCGC1050.046.00000434
GCCCTTT750.046.00000414
TTGACCC750.046.00000422
Produced by FastQC (version 0.11.5)