FastQCFastQC Report
Fri 17 Jun 2016
SRR1528012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337452
Sequences flagged as poor quality0
Sequence length59
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGTCGGTGCCCGTTACGGCCTTTCCGTCGGCCACCTTACAGTCCTTGCACAGCTTG92712.7473536977110817No Hit
TTAGGTCGGTGGGGGGATGGAGTTGCTGAATGGTAACAGTAATTCAAACCCAGGTTTGA9100.2696679824093501No Hit
AGGCGTCGGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGC9010.26700093642947736No Hit
AATGGTCGGTGGGTAACAGCGTGGTTTGCTGATGAATTCCCATTTTCGCCAGCAGCGGC8910.2640375520073966No Hit
GTTAGTCGGTGGGGAAGGGCATCGAGCACACCCAGCACAGCAGGAAGGACCACAACCAG8100.24003413818854238No Hit
CGTAGTCGGTGGGACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTA7510.2225501700982658No Hit
CCATGTCGGTGGGGCGTGCGCAGCCTCGCTATTTAATTTTCTTCCTTAGAGAGAGGCAC7270.21543804748527196No Hit
GTGCGTCGGTGGGGAGTGAGTGTCGGTGGGAGAGAGAGAGAGAGAGAGAGAAGAAAGGA7220.2139563552742316No Hit
GGTTGTCGGTGGGGACTCCTCTACACTCTCTGCACAACGTCTAAATTATGTGCCACTCG7150.21188198617877507No Hit
ATTCGTCGGTGGGATTGCCCAGCGCCATCGCGCCAAGATCGACAACTACATCCCCATCT6990.20714057110344583No Hit
CCAGGTCGGTGGGTTTCCGTCGGTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGC6820.20210281758590853No Hit
TATAGTCGGTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGA6790.20121380225928426No Hit
GCTTGTCGGTGGGGACAAATCTACAGCTAAGATCATACTGAGTGTGGAAAAGGTGAAAG6580.19499069497291466No Hit
GTAGGTCGGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCAC6420.19024927989758544No Hit
CTTGGTCGGTGGGGCTCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATGCACGC6150.18224814195796735No Hit
CTTGGTCGGTGGGTGCTGGGAAAACTGGCTAGCCTTATGTAGAAAGCTGAAACTGTATT6010.17809940376705427No Hit
GGATGTCGGTGGGTACACCCACTAGAATGGCGATAAAAAAGACAGTAACAAGTACTGGC5950.1763213731138058No Hit
CTAGGTCGGTGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATGCACGCTC5890.17454334246055736No Hit
TTGGGTCGGTGGGGCTCCTGCTGCTCCTGCAGGTCCTAAGCAGCGCCGCCACCTTCTCC5890.17454334246055736No Hit
ATGCGTCGGTGGGGGGGTGACTGGAAGTTTGTGGATATCAGGGTATACACTAAATCAGT5680.16832023517418773No Hit
GAGCGTCGGTGGTCCTAATAATTTAGAGTGCGGGGCAAAAGAATTGCTGTTTTCTCACC5650.1674312198475635No Hit
GCTTGTCGGTGGGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAA5580.16535685075210696No Hit
GTTGGTCGGTGGGGCAATCCCCAGCTTTCCTTCGCAACTCGAATGGAAGATTGGACTTG5540.16417149698327466No Hit
TCTTGTCGGTGGGGGTCCCCACTCTGGATCCCCTTTGCCACTCCTGGGGAGCCCACCTC5290.15676303592807272No Hit
GTTAGTCGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCA5000.1481692211040385No Hit
TTACGTCGGTGGGGCATCCCCTTGTTGTGTCATAACAATCCCCATTGTTTTTTTGTGTT4960.1469838673352062No Hit
TTTAGTCGGTGGGGGTGAAACCCTGTCTATACTAAAATACAAAAAAATTAGCCGGGCGT4910.1455021751241658No Hit
TAAAGTCGGTGGGGAGTTCGAATCTCTTCACTCCGACCATACACTGTCCCGGACCCGGA4840.14342780602870925No Hit
TCTCGTCGGTGGGGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGG4810.14253879070208503No Hit
TCGTGTCGGTGGGATATCTTATTGACCCAGATATATTTTGATCAACGGACCAAGTTACC4400.13038891457155388No Hit
CCCGGTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC4380.12979623768713774No Hit
TCTGGTCGGTGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATGCACGCTC4380.12979623768713774No Hit
GTGGGTCGGTGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAG4360.12920356080272158No Hit
AGACGTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC4350.1289072223605135No Hit
ACGCGTCGGTGGGGCCGTCGGTGGGGTAGTCGGTGGGCCTTTCCGGCGGTGACGACCTC4310.1277218685916812No Hit
GATTGTCGGTGGGGCTTCCTGCCTCCTGTGCCCACCGTTCTTAGGCATCAGCATGCCGG4290.12712919170726503No Hit
TAAGGTCGGTGGGTGCTCTCACTTTGGAAGAGCTGTGCAGTTGCTTTTGGCACCTGAGT4270.1265365148228489No Hit
ATGCGTCGGTGGGATGTCAGCAATATGAACAAGATGGCTGGCATGCATGAAACAAGAGT4210.12475848416960043No Hit
TTGTGTCGGTAGGCTCCACTGAAACCTTCCAATAACCTGCCATCTGCCAAGGACTGTTG4210.12475848416960043No Hit
CGAGGTCGGTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCT4170.12357313040076812No Hit
TTAAGTCGGTGGTTAATGAGAACATAGTTGAGAAGACTCATGATATCAATAACAAGGAA4150.12298045351635195No Hit
TCTGGTCGGTGGGCCCTCTGAAGTTGGGGGAAAGGAGCTGGGGATGGGTATATTAGGTT4120.12209143818972773No Hit
TTTTGTCGGTGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4100.12149876130531156No Hit
AAGCGTCGGTGGCACTTTAGCTGTCTTCAGACACATCAAAAGAAGGTTTCAGGTCCCAT4040.11972073065206312No Hit
CGCGGTCGGTGGGATTCCAGCTCCAGCCACTGCTGCTCGTTGCCGTTGATTACCGCCGT3940.11675734622998232No Hit
CTGGGTCGGTGGGGAGGAGGCTGGGGCCCTGGAGACGATGCGCCCCGCGGAGCCGCCTG3920.1161646693455662No Hit
CTTGGTCGGTGGGGGTGGTGCACACCTGTAGTCCCAGCTACTCGGGAGGCTTAGGCACG3890.11527565401894195No Hit
ATTCGTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC3860.11438663869231772No Hit
TTTAGTCGGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCA3850.11409030025010965No Hit
GTTGGTCGGTGGACTTGGGAACTGCGTTTGAAACTCCAAAGCTAGAGTATAGGAGAGGG3840.11379396180790156No Hit
CTAAGTCGGTGGGGTTCGGCATTCAGTCCGGGGCTGCAGCAGAAAAGCCATTTGCTGAG3830.1134976233656935No Hit
TCCTGTCGGTGGGGCTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGACT3770.11171959271244503No Hit
CACGGTCGGTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACT3770.11171959271244503No Hit
TCTGGTCGGTGGGGGGCTGTTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTCC3710.10994156205919657No Hit
TGTTGTCGGTGGGCTTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAA3710.10994156205919657No Hit
AGGGGTCGGTGGGGCCAGGCACAGTAGCTCATGGTGTAACCCCAGCACTTGGGAAGGCC3700.10964522361698847No Hit
CGGCGTCGGTGGGACATTCTCTCTCGTAGGGCCAGTTGTTATCACGGTATGGAAGTGTG3660.10845986984815618No Hit
ATAAGTCGGTGGGAAACTGGTATCTCAGAAACTAAGCTGAGCACAGTGGCTCCTATCTG3630.10757085452153196No Hit
CCTCGTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTTTTC3620.10727451607932387No Hit
ATTGGTCGGTGGCTTTTTTTCCTACTTGGGCCCGGCAGAATGGCTCCCGCAAAGAAGGG3580.10608916231049158No Hit
GTAGGTCGGTGGGGTGGATGGTGGATGTGTGGATGGATGAGTGGGTGGATAGATGGATG3550.10520014698386733No Hit
GTATGTCGGTGGGGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCCGCTCGTGG3510.10401479321503504No Hit
CTAGGTCGGTGGGGAACTAAAGGTAAGGCCAGATACTCTAAAACTCCTATAGGAAAGCA3440.10194042411957849No Hit
ACAAGTCGGTGGGCAACGCAGAGTTTTGGTCGGTGGGAAGCAGTGGTATCAACGGAATG3440.10194042411957849No Hit
GGGGGTCGGTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCAAGAA3430.1016440856773704No Hit
TTTCGTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3410.10105140879295427No Hit
TTATGTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTAC3410.10105140879295427No Hit
CGGAGTCGGTGGGGAGTCGGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCT3400.10075507035074618No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAT150.00669218653.00000428
TCGCCTC150.00669218653.00000453
CTTAACT306.9955786E-753.00000430
TGTCATA600.053.00000428
ATGGGTG650.053.00000448
GTTTGCT1200.053.00000424
TTTAGCT150.00669218653.00000416
AGCGTTA306.9955786E-753.00000443
TTTAGCA150.00669218653.00000453
GTCATAA600.053.00000429
AACAATT150.00669218653.00000416
AACGTGT150.00669218653.00000440
TAATATG150.00669218653.00000423
AACGTAC150.00669218653.00000415
CGGGTAG150.00669218653.00000415
AGCGTCA600.053.00000439
AGCCGTG306.9955786E-753.00000435
AGGTAGT150.00669218653.00000438
TAATAAT650.053.00000416
GACAGAT150.00669218653.00000450
Produced by FastQC (version 0.11.5)