FastQCFastQC Report
Fri 17 Jun 2016
SRR1528011_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528011_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2564383
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGCCATCTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCC36610.14276338596847662No Hit
TTTGCCATCTGGGTGGCCAACTTAGCCATAGGCCCCGTTGGCTGTCCTGTCT28940.11285365719551253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAAT2150.046.00000434
CTACGAA750.046.00000440
TACTCGC750.046.00000446
AGCGTTA253.4183417E-546.044
AGGTATA501.6370905E-1146.034
AACGTAC206.3129154E-446.021
CTATGCG600.046.041
TCGTTTA301.8623068E-646.023
TCGTTGA206.3129154E-446.029
ACCGGAT900.046.042
ACCGGAC501.6370905E-1146.025
CATATCG600.046.033
CGTATGT405.6152203E-946.039
CTCGTAC600.046.016
CTACTAC206.3129154E-446.034
CGAGTAT551.8189894E-1246.037
TTTACGC405.6152203E-946.028
CGAAACT253.4183417E-546.034
ACGTTAT206.3129154E-446.037
CGCATAA453.110472E-1046.044
Produced by FastQC (version 0.11.5)