##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528011_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2564383
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.932376715958576	33.0	31.0	34.0	30.0	34.0
2	32.411046633829656	34.0	31.0	34.0	30.0	34.0
3	32.489450288821914	34.0	31.0	34.0	31.0	34.0
4	36.06537712970332	37.0	35.0	37.0	35.0	37.0
5	36.00922015159202	37.0	35.0	37.0	35.0	37.0
6	35.938718592347556	37.0	35.0	37.0	35.0	37.0
7	36.019280661274074	37.0	35.0	37.0	35.0	37.0
8	36.051046197077426	37.0	36.0	37.0	35.0	37.0
9	37.684168082536814	39.0	38.0	39.0	35.0	39.0
10	38.02046262200303	39.0	38.0	39.0	35.0	39.0
11	38.2195284401745	39.0	39.0	39.0	37.0	39.0
12	38.16489892500457	39.0	39.0	39.0	37.0	39.0
13	38.159627481542344	39.0	39.0	39.0	37.0	39.0
14	38.817299911908634	40.0	39.0	40.0	36.0	40.0
15	38.25210664709601	40.0	38.0	40.0	34.0	40.0
16	37.59916634917639	40.0	36.0	40.0	33.0	40.0
17	37.542902132793735	40.0	37.0	40.0	33.0	40.0
18	37.53419048558659	40.0	37.0	40.0	33.0	40.0
19	37.693083287480846	40.0	37.0	40.0	33.0	40.0
20	37.74293738493821	40.0	37.0	40.0	33.0	40.0
21	37.5460693663934	40.0	37.0	40.0	33.0	40.0
22	37.648068560741514	40.0	37.0	40.0	33.0	40.0
23	37.60015840067572	40.0	37.0	40.0	33.0	40.0
24	37.57801974198082	40.0	37.0	40.0	33.0	40.0
25	37.67213360874721	40.0	37.0	40.0	33.0	40.0
26	37.64314651906521	40.0	37.0	40.0	33.0	40.0
27	37.62755290453883	40.0	37.0	40.0	33.0	40.0
28	37.61994210693177	40.0	37.0	40.0	33.0	40.0
29	37.607300859504996	40.0	37.0	40.0	33.0	40.0
30	37.562643333698595	40.0	36.0	40.0	33.0	40.0
31	37.53156256300248	40.0	36.0	40.0	33.0	40.0
32	37.476109067951235	40.0	36.0	40.0	33.0	40.0
33	37.43081825140784	40.0	36.0	40.0	33.0	40.0
34	37.360571724270514	40.0	36.0	40.0	33.0	40.0
35	37.28165956489339	40.0	35.0	40.0	33.0	40.0
36	37.22191497915873	40.0	35.0	40.0	33.0	40.0
37	37.109999949305546	40.0	35.0	40.0	33.0	40.0
38	36.90723616558057	39.0	35.0	40.0	32.0	40.0
39	36.796497247096084	39.0	35.0	40.0	32.0	40.0
40	36.67848913364345	39.0	35.0	40.0	32.0	40.0
41	36.540308526456464	39.0	35.0	40.0	31.0	40.0
42	36.40992706627676	39.0	35.0	40.0	31.0	40.0
43	36.25162582968301	38.0	35.0	40.0	31.0	40.0
44	36.097616463687366	38.0	35.0	40.0	31.0	40.0
45	35.931061779773145	38.0	35.0	40.0	30.0	40.0
46	35.80209430494587	38.0	35.0	40.0	30.0	40.0
47	35.69941424506402	37.0	35.0	40.0	30.0	40.0
48	35.530333417434136	37.0	35.0	40.0	30.0	40.0
49	35.40532946911596	37.0	35.0	40.0	30.0	40.0
50	35.25991749282381	36.0	34.0	40.0	29.0	40.0
51	35.09067444293618	36.0	34.0	40.0	29.0	40.0
52	34.413993931483716	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	7.0
14	7.0
15	19.0
16	35.0
17	134.0
18	276.0
19	581.0
20	1104.0
21	1930.0
22	2975.0
23	4743.0
24	6878.0
25	9796.0
26	13596.0
27	18800.0
28	24978.0
29	32786.0
30	42702.0
31	53950.0
32	67070.0
33	86532.0
34	129298.0
35	250055.0
36	211713.0
37	395023.0
38	764445.0
39	444948.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.280935024136408	21.813005311609068	32.3827992932413	20.523260371013222
2	22.829546132539484	18.852020154555696	34.82950869663385	23.48892501627097
3	20.25181105942443	22.072872889892032	32.23707223141005	25.438243819273488
4	28.790668164622836	21.27533211692637	40.34155584403734	9.592443874413457
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	0.0	100.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	100.0	0.0
11	99.28013873122697	0.44205565237329997	0.17107428960494592	0.10673132679478844
12	99.19224234445478	0.4286021237857216	0.09448666599334031	0.28466886576615114
13	98.45475500344527	0.8525637551021046	0.27199525187930196	0.42068598957332043
14	64.4122192355822	22.771481483070197	5.6624147017040745	7.153884579643524
15	36.2782002532383	26.928855791042132	16.742623859228516	20.05032009649105
16	31.190114737151198	17.0218723178246	27.383234095686955	24.404778849337248
17	24.668000060833347	16.382693224841997	28.89736049568259	30.051946218642065
18	24.50211220398825	18.739010514420038	31.617897950501156	25.140979331090556
19	25.462460170731127	18.72524501995217	28.202612480273032	27.60968232904367
20	27.126291197531728	17.833373563933314	26.919067861547987	28.12126737698698
21	28.35988227967507	19.189918198646613	22.42617424932235	30.024025272355964
22	29.492045454988585	19.41749730831939	22.998904609802825	28.0915526268892
23	27.916383785105424	20.386229358095108	22.30240178631663	29.394985070482843
24	28.419584750015893	23.451138149020643	20.857921769096112	27.271355331867355
25	30.870661675732524	20.443124135513298	22.985919030035685	25.70029515871849
26	29.16580713567357	19.693197155027153	22.060004297330003	29.080991411969272
27	30.719592198201283	20.294940342374755	21.99878099332276	26.9866864661012
28	31.311508460319693	21.780014919768227	20.737229969158275	26.171246650753805
29	30.707776490485234	20.469836214013274	20.55469093345261	28.267696362048884
30	29.99399855637789	20.608622035008032	20.686106560525474	28.711272848088605
31	27.79151944151868	19.82344291004893	20.302973463792267	32.082064184640124
32	29.12185894228748	21.653590746780026	20.571888052603686	28.65266225832881
33	30.39386082344174	20.84396129595306	20.01151934012977	28.750658540475428
34	27.63947507061153	23.10392012425601	22.051932180177456	27.204672624955005
35	30.0972202670194	24.021684748339077	19.672724394133017	26.20837059050852
36	33.40881607778557	22.48884039552594	18.805654225597344	25.296689301091142
37	32.556525292828724	21.191725261008205	20.18817001984493	26.063579426318146
38	29.76462564289344	24.20387282242941	23.065314346569917	22.966187188107238
39	31.70130982774414	23.717479019319658	22.627002284760117	21.954208868176085
40	30.248250748815604	24.75550649025516	20.276456363967473	24.719786396961766
41	30.710389204732678	24.126115326766712	20.293614487383515	24.869880981117095
42	30.998723669592255	25.216826035736474	19.19319384038968	24.591256454281595
43	31.119493461000168	22.90515886277518	20.405259276792897	25.570088399431757
44	29.569179018890708	22.695712769894357	21.23481554822349	26.50029266299145
45	28.38909008521738	24.133017571868166	21.57478036627134	25.903111976643117
46	26.784493579937163	23.080990632054572	21.80902774663535	28.325488041372914
47	28.885193826351212	20.056754392772064	23.75136631306634	27.30668546781039
48	29.08559290870357	20.00758077089109	22.422196684348634	28.484629636056702
49	28.407574063624665	21.865064617882744	22.928985256882456	26.798376061610142
50	30.17072722756312	21.02260075815508	22.354773058470595	26.451898955811203
51	28.721996675223632	21.78719013501493	22.063552909218316	27.427260280543113
52	29.10676759282837	22.45401720413838	22.502059949703302	25.93715525332994
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	5.5
10	11.0
11	16.0
12	21.0
13	133.0
14	380.0
15	515.0
16	814.5
17	1114.0
18	1219.5
19	1325.0
20	1651.0
21	1977.0
22	2380.5
23	2784.0
24	3684.5
25	4585.0
26	6630.5
27	8676.0
28	10319.5
29	11963.0
30	13727.0
31	15491.0
32	16881.5
33	18272.0
34	19767.5
35	21263.0
36	23896.5
37	26530.0
38	29311.5
39	37397.5
40	42702.0
41	47084.5
42	51467.0
43	55030.5
44	58594.0
45	67255.0
46	75916.0
47	82604.5
48	89293.0
49	108021.0
50	126749.0
51	133295.5
52	139842.0
53	147260.5
54	154679.0
55	167795.0
56	180911.0
57	196991.0
58	213071.0
59	226129.0
60	239187.0
61	240097.0
62	241007.0
63	227640.0
64	186871.0
65	159469.0
66	141977.5
67	124486.0
68	106032.5
69	87579.0
70	80797.0
71	74015.0
72	68864.0
73	63713.0
74	49530.0
75	35347.0
76	30901.0
77	26455.0
78	19235.5
79	12016.0
80	8437.5
81	4859.0
82	3194.0
83	1529.0
84	914.0
85	299.0
86	171.5
87	44.0
88	29.5
89	8.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2564383.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.8850446106852665
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.29526563848357	5.046411736729863
2	8.603299608970215	1.1846820321370208
3	4.115192070548626	0.849998429617967
4	2.4814824885222393	0.6834047053643962
5	1.604408350867526	0.5523211534739448
6	1.0510043622322658	0.43417271519963807
7	0.7646008348281303	0.3685017600011213
8	0.5291634763300285	0.29146513127020346
9	0.4038503300790981	0.25024747847711043
>10	2.816608307510849	4.013300562047151
>50	0.7327773590527015	3.7199558303163944
>100	3.0194301909955366	52.4127731520189
>500	0.4968754720792806	22.494394793716197
>1k	0.08604150949996717	7.698370519630086
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCGCCATCTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCC	3661	0.14276338596847662	No Hit
TTTGCCATCTGGGTGGCCAACTTAGCCATAGGCCCCGTTGGCTGTCCTGTCT	2894	0.11285365719551253	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.001208867786130231	0.0	0.0	0.0	0.0
12	0.0021057696919687895	0.0	0.0	0.0	0.0
13	0.003041667332843807	0.0	0.0	0.0	0.0
14	0.010996797280281455	0.0	0.0	0.0	0.0
15	0.024255347192677537	0.0	0.0	0.0	0.0
16	0.0326784259605527	0.0	0.0	0.0	0.0
17	0.037669880045219456	0.0	0.0	0.0	0.0
18	0.046287937488276906	0.0	0.0	0.0	0.0
19	0.054672020521115604	0.0	0.0	0.0	0.0
20	0.06609777088679811	0.0	0.0	0.0	0.0
21	0.07814745301306396	0.0	0.0	0.0	0.0
22	0.09191294748093401	0.0	0.0	0.0	0.0
23	0.1037286551969811	0.0	0.0	0.0	0.0
24	0.11827406436558034	0.0	0.0	0.0	0.0
25	0.1338333626451275	0.0	0.0	0.0	0.0
26	0.15114746900131532	0.0	0.0	0.0	0.0
27	0.16799362653706565	0.0	0.0	0.0	0.0
28	0.1846058096625972	0.0	0.0	0.0	0.0
29	0.2074573103939622	0.0	0.0	0.0	0.0
30	0.23588520123554088	0.0	0.0	0.0	0.0
31	0.26212933091507784	0.0	0.0	0.0	0.0
32	0.28143221975812505	0.0	0.0	0.0	0.0
33	0.29706950950774513	0.0	0.0	0.0	0.0
34	0.31247282484714645	0.0	0.0	0.0	0.0
35	0.3311127861945739	0.0	0.0	0.0	0.0
36	0.34811492667047006	0.0	0.0	0.0	0.0
37	0.36308928892447034	0.0	0.0	0.0	0.0
38	0.3825481607076634	0.0	0.0	0.0	0.0
39	0.40364485336238776	0.0	0.0	0.0	0.0
40	0.4279002005550653	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCAAT	215	0.0	46.000004	34
CTACGAA	75	0.0	46.000004	40
TACTCGC	75	0.0	46.000004	46
AGCGTTA	25	3.4183417E-5	46.0	44
AGGTATA	50	1.6370905E-11	46.0	34
AACGTAC	20	6.3129154E-4	46.0	21
CTATGCG	60	0.0	46.0	41
TCGTTTA	30	1.8623068E-6	46.0	23
TCGTTGA	20	6.3129154E-4	46.0	29
ACCGGAT	90	0.0	46.0	42
ACCGGAC	50	1.6370905E-11	46.0	25
CATATCG	60	0.0	46.0	33
CGTATGT	40	5.6152203E-9	46.0	39
CTCGTAC	60	0.0	46.0	16
CTACTAC	20	6.3129154E-4	46.0	34
CGAGTAT	55	1.8189894E-12	46.0	37
TTTACGC	40	5.6152203E-9	46.0	28
CGAAACT	25	3.4183417E-5	46.0	34
ACGTTAT	20	6.3129154E-4	46.0	37
CGCATAA	45	3.110472E-10	46.0	44
>>END_MODULE