FastQCFastQC Report
Fri 17 Jun 2016
SRR1528010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2540027
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGCCATCTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCC35080.13810876813514186No Hit
TTTGCCATCTGGGTGGCCAACTTAGCCATAGGCCCCGTTGGCTGTCCTGTCT27910.10988072174035945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATA750.046.00000434
TTTACGC750.046.00000428
TCACGGC551.8189894E-1246.022
ATTTACG650.046.027
AACGTAA206.312905E-446.042
TCGCAAT206.312905E-446.037
CATATCG800.046.033
CGTATGG405.6152203E-946.042
AATCACG206.312905E-446.016
CGACGTT253.4183315E-546.032
TTTACGG650.046.043
ACGTTGT253.4183315E-546.034
CATGCGA301.8622995E-646.028
AACCCGT551.8189894E-1246.015
GGCGTAT405.6152203E-946.040
ATTACGA405.6152203E-946.033
TTCACGG253.4183315E-546.023
GAATCGT453.110472E-1046.019
ATTCGCT206.312905E-446.033
CGTCTAG650.046.021
Produced by FastQC (version 0.11.5)