FastQCFastQC Report
Fri 17 Jun 2016
SRR1528009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597157
Sequences flagged as poor quality0
Sequence length59
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGCCATCTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCAC34900.13437770608399877No Hit
TTTGCCATCTGGGTGGCCAACTTAGCCATAGGCCCCGTTGGCTGTCCTGTCTGCCCTGC26270.10114906414976069No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG251.4792526E-553.00000427
AACCGTA307.013932E-753.00000447
AACGTAC251.4792526E-553.00000421
TCGCATA1200.053.00000430
TCGTTGA150.00669721153.00000429
CGAACTA251.4792526E-553.00000434
CATATCG503.6379788E-1253.00000433
CGAAACG150.00669721153.00000449
ACGTTGT457.6397555E-1153.00000434
ACGTTAT150.00669721153.00000437
TAGGTTA251.4792526E-553.00000450
GTTTACG150.00669721153.00000427
GACCGTA251.4792526E-553.00000421
CGCATAA251.4792526E-553.00000444
ACATTAC150.00669721153.00000439
GAATCGT457.6397555E-1153.00000419
TAAACGA307.013932E-753.00000437
TAACGTG150.00669721153.00000448
TAACGAC307.013932E-753.00000443
CCGTCTA150.00669721153.00000441