##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528009_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2597157
Sequences flagged as poor quality	0
Sequence length	59
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.537753782308886	31.0	31.0	34.0	30.0	34.0
2	32.00210114367364	33.0	31.0	34.0	30.0	34.0
3	32.148079226631275	34.0	31.0	34.0	30.0	34.0
4	35.8449623954193	37.0	35.0	37.0	35.0	37.0
5	35.65510286825171	37.0	35.0	37.0	33.0	37.0
6	35.572466739592564	37.0	35.0	37.0	33.0	37.0
7	35.72741347558118	37.0	35.0	37.0	33.0	37.0
8	35.75796726959518	37.0	35.0	37.0	33.0	37.0
9	37.41627826119099	39.0	37.0	39.0	34.0	39.0
10	37.74546937285655	39.0	37.0	39.0	35.0	39.0
11	37.99520360147654	39.0	37.0	39.0	35.0	39.0
12	37.93374755550011	39.0	37.0	39.0	35.0	39.0
13	37.927953527645805	39.0	38.0	39.0	35.0	39.0
14	38.35284813355527	40.0	38.0	40.0	35.0	40.0
15	37.76381327736444	40.0	38.0	40.0	33.0	40.0
16	37.139132520675496	39.0	36.0	40.0	32.0	40.0
17	37.01846519097613	39.0	36.0	40.0	32.0	40.0
18	36.96869499995572	39.0	36.0	40.0	32.0	40.0
19	37.11929775519924	40.0	36.0	40.0	32.0	40.0
20	37.25446555599065	40.0	36.0	40.0	32.0	40.0
21	37.21664458482872	40.0	36.0	40.0	32.0	40.0
22	37.235469784845506	40.0	36.0	40.0	32.0	40.0
23	37.18451637694602	40.0	36.0	40.0	32.0	40.0
24	37.139789392786035	39.0	36.0	40.0	32.0	40.0
25	37.08011144493768	39.0	36.0	40.0	32.0	40.0
26	36.9255135519339	39.0	35.0	40.0	31.0	40.0
27	36.81217346506199	39.0	35.0	40.0	31.0	40.0
28	36.82248319989897	39.0	35.0	40.0	31.0	40.0
29	36.777483609962744	39.0	35.0	40.0	31.0	40.0
30	36.73508186066533	39.0	35.0	40.0	31.0	40.0
31	36.736675911390805	39.0	35.0	40.0	31.0	40.0
32	36.609114504822	39.0	35.0	40.0	31.0	40.0
33	36.502185659165	39.0	35.0	40.0	31.0	40.0
34	36.435056871802516	39.0	35.0	40.0	30.0	40.0
35	36.30866443576572	39.0	35.0	40.0	30.0	40.0
36	36.160778112374416	38.0	35.0	40.0	30.0	40.0
37	36.036918445823645	38.0	35.0	40.0	30.0	40.0
38	35.81606387291951	38.0	35.0	40.0	29.0	40.0
39	35.66963722254758	38.0	34.0	40.0	29.0	40.0
40	35.51597881837717	38.0	34.0	40.0	28.0	40.0
41	35.39112729804167	38.0	34.0	40.0	28.0	40.0
42	35.29562517783869	38.0	34.0	40.0	28.0	40.0
43	35.10330372788399	37.0	34.0	40.0	27.0	40.0
44	34.94879901369074	37.0	34.0	40.0	27.0	40.0
45	34.72551948149457	37.0	33.0	40.0	26.0	40.0
46	34.54719217975656	36.0	33.0	40.0	26.0	40.0
47	34.467737606929425	36.0	33.0	40.0	26.0	40.0
48	34.26898181357538	36.0	33.0	39.0	25.0	40.0
49	34.18249378069943	36.0	33.0	39.0	26.0	40.0
50	33.97430190011617	35.0	33.0	39.0	24.0	40.0
51	33.80128617561434	35.0	33.0	39.0	24.0	40.0
52	33.600505860831674	35.0	33.0	39.0	24.0	40.0
53	33.384278270431864	35.0	32.0	38.0	24.0	40.0
54	33.231780365992506	35.0	32.0	38.0	23.0	40.0
55	33.079850005217246	35.0	32.0	38.0	23.0	40.0
56	32.883957727622935	35.0	32.0	38.0	23.0	40.0
57	32.732047773777246	35.0	32.0	37.0	23.0	40.0
58	32.58466392289723	35.0	31.0	37.0	23.0	40.0
59	32.01596899994879	34.0	31.0	36.0	22.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	6.0
14	15.0
15	51.0
16	190.0
17	583.0
18	1225.0
19	2215.0
20	3788.0
21	5887.0
22	8796.0
23	12065.0
24	16857.0
25	22045.0
26	28483.0
27	35744.0
28	44859.0
29	54682.0
30	66002.0
31	80252.0
32	99390.0
33	126896.0
34	185390.0
35	265007.0
36	293830.0
37	440236.0
38	634201.0
39	168461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.331891757025087	22.150412932294813	31.896839505659457	20.620855805020643
2	22.438035128411567	19.38954787869967	34.60738030084435	23.565036692044416
3	20.175599703829995	22.266231883555747	32.02898400058217	25.52918441203208
4	28.819667043617308	21.511907058371904	40.12094763620374	9.547478261807045
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	0.0	100.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	100.0	0.0
11	99.27439889078713	0.4489139470582641	0.16556565506051427	0.11112150709410328
12	99.18522445889872	0.43305044708502416	0.08567060058363819	0.2960544934326265
13	98.44526149170035	0.8690656745048528	0.2649050480968228	0.4207677856979767
14	64.06489865649246	23.0087745946818	5.737889546146036	7.1884372026797
15	36.314131182673975	27.030633881586674	16.791822750800204	19.863412184939147
16	31.23257469610039	17.153063907957815	27.274785467339864	24.339575928601928
17	24.68372146928353	16.501120263426508	28.814738577606207	30.00041968968376
18	24.41893193210884	18.81334089544837	31.6708231346815	25.09690403776129
19	25.525757588008734	18.7990175411036	28.192057700015827	27.48316717087184
20	27.120847911774298	18.026441990222388	26.949044666918482	27.903665431084836
21	28.035039853193318	19.37791977920472	22.53414021562809	30.052900151973873
22	29.40781015548925	19.588188161131576	22.97419832532265	28.029803358056522
23	27.865392812217358	20.530949803958716	22.377199376087006	29.226458007736923
24	28.389696887789224	23.545592353484984	20.895040230529	27.169670528196793
25	30.78562443471842	20.60141146646121	23.017976964811908	25.594987134008456
26	29.194191956820475	19.686256934024396	22.125462573113598	28.994088536041524
27	30.666686688559835	20.413706217991443	22.067399082920286	26.852208010528432
28	31.11667873755803	21.936871740907463	20.90647581182039	26.039973709714122
29	30.69005839847187	20.553897973822917	20.62894156957011	28.127102058135105
30	29.92487554660731	20.738946471083572	20.711916915303927	28.62426106700519
31	27.699249602546168	19.94742712897218	20.344861708398838	32.008461560082814
32	29.005293095488643	21.878808250714147	20.657203241852535	28.458695411944678
33	30.372210844396392	21.00462159199463	19.996596278161082	28.626571285447895
34	27.517127381979606	23.255659938925525	22.1070963364941	27.120116342600774
35	30.119588457686618	24.135660647392516	19.711746344175573	26.033004550745297
36	33.32393844499967	22.549041124583535	18.942790135521264	25.18423029489553
37	32.3499503495553	21.3752191338452	20.248949139385875	26.025881377213622
38	29.63290243909013	24.35609399046727	23.12975303379811	22.881250536644494
39	31.57541111299779	23.880458516755052	22.688424303960062	21.855706066287098
40	30.10503408149758	24.94442962054277	20.31317321209307	24.637363085866586
41	30.70053138874546	24.27569838866114	20.327304048234282	24.696466174359117
42	31.001937888237023	25.32684778009185	19.21839919573595	24.452815135935175
43	31.02715777290322	23.046315644375753	20.404272826017063	25.522253756703968
44	29.456709779193176	22.805898911771603	21.212079208149525	26.5253121008857
45	28.323509129405732	24.283553131366336	21.546213802246072	25.846723936981864
46	26.689530128521305	23.217695349183742	21.84492504688781	28.24784947540715
47	28.788286576437233	20.26361902649705	23.695024983087276	27.253069413978437
48	29.040870459506294	20.095666145712407	22.32903132155661	28.534432073224686
49	28.279537971712916	22.059698354777936	22.943010376346134	26.71775329716301
50	30.079198138579994	21.163603124493434	22.417936227960034	26.339262508966538
51	28.564888453027677	21.989352203197573	22.064434302585482	27.38132504118927
52	28.959897303089495	22.745140166728465	22.497831282436913	25.79713124774513
53	28.273415892839747	23.435510444690095	21.824210088184888	26.466863574285266
54	27.40754602051397	22.074137220044843	22.874319881316378	27.64399687812481
55	26.23845997758318	23.22270082247627	21.452380429831543	29.086458770109008
56	26.757643068940386	23.791746128555186	23.319807004351294	26.130803798153135
57	27.339048043687768	22.098047980926836	23.353497689974077	27.20940628541132
58	27.682230993351574	22.029511500459925	22.414432396655265	27.873825109533236
59	26.173311817498902	22.11029213867317	22.830194709060716	28.886201334767208
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	2.0
9	6.0
10	8.0
11	93.5
12	179.0
13	249.0
14	514.5
15	710.0
16	816.0
17	922.0
18	1001.5
19	1001.0
20	921.0
21	1444.5
22	1968.0
23	2186.0
24	2404.0
25	3361.0
26	6167.5
27	8017.0
28	8920.5
29	9824.0
30	11750.5
31	15025.0
32	16373.0
33	20171.0
34	23969.0
35	23901.5
36	27718.0
37	31602.0
38	33534.5
39	35467.0
40	39795.0
41	44123.0
42	45824.0
43	52200.0
44	56875.0
45	63449.0
46	70023.0
47	74063.5
48	85749.0
49	93394.0
50	104136.0
51	114878.0
52	117697.5
53	137277.0
54	154037.0
55	157038.5
56	160040.0
57	182067.5
58	211468.5
59	218842.0
60	225674.5
61	232507.0
62	207346.5
63	182186.0
64	163149.0
65	134957.5
66	125803.0
67	112312.5
68	98822.0
69	86530.5
70	71059.5
71	67880.0
72	61640.0
73	55400.0
74	43146.5
75	29537.5
76	28182.0
77	19244.5
78	10307.0
79	7440.5
80	4574.0
81	3888.0
82	1847.0
83	492.0
84	362.5
85	233.0
86	185.5
87	122.0
88	106.0
89	68.5
90	31.0
91	15.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	2597157.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.237941261614807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6930539543021	9.068238005587615
2	7.425065348155199	1.6688489649243927
3	3.1145669992146368	1.0500396297761394
4	1.7510834820583843	0.787142932622242
5	1.067390858429306	0.5997637885106574
6	0.7082074937755829	0.4775276529651261
7	0.47883199469461074	0.3766760081391922
8	0.3307065498076488	0.29731686252557193
9	0.23942783365445983	0.24216083379040507
>10	1.607785313977323	3.739313726823042
>50	0.44666863463473955	3.7124084762249354
>100	1.815513993449178	50.9204434204945
>500	0.27569170513231633	20.42050350255854
>1k	0.046005838714483674	6.639616195057639
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCGCCATCTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCAC	3490	0.13437770608399877	No Hit
TTTGCCATCTGGGTGGCCAACTTAGCCATAGGCCCCGTTGGCTGTCCTGTCTGCCCTGC	2627	0.10114906414976069	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	9.2408737708194E-4	0.0	0.0	0.0	0.0
12	0.0018096711134521326	0.0	0.0	0.0	0.0
13	0.0027337584905340723	0.0	0.0	0.0	0.0
14	0.010588501195730562	0.0	0.0	0.0	0.0
15	0.02418028636697743	0.0	0.0	0.0	0.0
16	0.03342116013779683	0.0	0.0	0.0	0.0
17	0.03838812978961226	0.0	0.0	0.0	0.0
18	0.04635838341694399	0.0	0.0	0.0	0.0
19	0.056446337283421834	0.0	0.0	0.0	0.0
20	0.06699633483844065	0.0	0.0	0.0	0.0
21	0.07743082147132423	0.0	0.0	0.0	0.0
22	0.09063757023545362	0.0	0.0	0.0	0.0
23	0.10141858963474291	0.0	0.0	0.0	0.0
24	0.1133162146146729	0.0	0.0	0.0	0.0
25	0.12582989784599083	0.0	0.0	0.0	0.0
26	0.142270952429907	0.0	0.0	0.0	0.0
27	0.1575954014331825	0.0	0.0	0.0	0.0
28	0.17334339048428724	0.0	0.0	0.0	0.0
29	0.1939043346243604	0.0	0.0	0.0	0.0
30	0.22154994865539512	0.0	0.0	0.0	0.0
31	0.2452296876931198	0.0	0.0	0.0	0.0
32	0.2657136245517695	0.0	0.0	0.0	0.0
33	0.28084555535148625	0.0	0.0	0.0	0.0
34	0.29663204804330273	0.0	0.0	0.0	0.0
35	0.3124185407351192	0.0	0.0	0.0	0.0
36	0.3285130625526297	0.0	0.0	0.0	0.0
37	0.34368349699305817	0.0	0.0	0.0	0.0
38	0.361241157157615	0.0	0.0	0.0	0.0
39	0.38030045930993006	0.0	0.0	0.0	0.0
40	0.4051353075690072	0.0	0.0	0.0	0.0
41	0.42827599563676744	0.0	0.0	0.0	0.0
42	0.4494529980282286	0.0	0.0	0.0	0.0
43	0.47005244580901345	0.0	0.0	0.0	0.0
44	0.48915025160204023	0.0	0.0	0.0	0.0
45	0.5079015246286612	0.0	0.0	0.0	0.0
46	0.5272303522659585	0.0	0.0	0.0	0.0
47	0.5442104578198391	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	25	1.4792526E-5	53.000004	27
AACCGTA	30	7.013932E-7	53.000004	47
AACGTAC	25	1.4792526E-5	53.000004	21
TCGCATA	120	0.0	53.000004	30
TCGTTGA	15	0.006697211	53.000004	29
CGAACTA	25	1.4792526E-5	53.000004	34
CATATCG	50	3.6379788E-12	53.000004	33
CGAAACG	15	0.006697211	53.000004	49
ACGTTGT	45	7.6397555E-11	53.000004	34
ACGTTAT	15	0.006697211	53.000004	37
TAGGTTA	25	1.4792526E-5	53.000004	50
GTTTACG	15	0.006697211	53.000004	27
GACCGTA	25	1.4792526E-5	53.000004	21
CGCATAA	25	1.4792526E-5	53.000004	44
ACATTAC	15	0.006697211	53.000004	39
GAATCGT	45	7.6397555E-11	53.000004	19
TAAACGA	30	7.013932E-7	53.000004	37
TAACGTG	15	0.006697211	53.000004	48
TAACGAC	30	7.013932E-7	53.000004	43
CCGTCTA	15	0.006697211	53.000004	41
>>END_MODULE