FastQCFastQC Report
Fri 17 Jun 2016
SRR1528008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658989
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGCTGTGGGGAGCCAAGGTGGGTGGATCACTTGAGGCCAGGAGTTTGAG20300.12236368053073288No Hit
ACCTGCTGTGGGGGGGAGGGGCTGGAGAGATGGCTCATCGGTTAAGAGCTCT18630.11229730878263809No Hit
CACTGCTGTGGGGCTGGGATACATGAGGATCAATGCCCGTTTTCTCCCCGTG18610.11217675343236151No Hit
GTGGGCTGTGGGGGGCTCCTGAGACCCACCTCTGCTCTGTGCTGCTGCGTAG18130.10928342502572351No Hit
TAGGGCTGTGGGGGGTATATGTGTGTGTGTGAGTGAGTGAGGGAGAGTGAGT17300.10428037798924526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG551.8189894E-1246.00000423
TTAGGCG750.046.00000416
AATCCCC351.01994374E-746.00000420
AGATATA351.01994374E-746.00000434
CGGCCTA700.046.00000442
CTATCCA351.01994374E-746.00000417
GTTTAGT700.046.00000434
CGGCATG700.046.00000436
GAACTCC551.8189894E-1246.00000440
TTCACCG351.01994374E-746.00000416
ACCGAGT351.01994374E-746.00000428
CCTAACG351.01994374E-746.00000438
ATCGTAC351.01994374E-746.00000420
TTATACC750.046.00000429
ATTCCCG1450.046.00000441
CTAACGC351.01994374E-746.00000439
GCATTAC351.01994374E-746.00000414
ATTCACC351.01994374E-746.00000415
AGGTTAA351.01994374E-746.00000433
TAACACC351.01994374E-746.00000434
Produced by FastQC (version 0.11.5)