FastQCFastQC Report
Fri 17 Jun 2016
SRR1528007_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528007_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648521
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGCTGTGGGGAGCCAAGGTGGGTGGATCACTTGAGGCCAGGAGTTTGAG19870.12053228317989277No Hit
CACTGCTGTGGGGCTGGGATACATGAGGATCAATGCCCGTTTTCTCCCCGTG18090.10973472585426573No Hit
ACCTGCTGTGGGGGGGAGGGGCTGGAGAGATGGCTCATCGGTTAAGAGCTCT18020.10931010281337028No Hit
TAGGGCTGTGGGGGGTATATGTGTGTGTGTGAGTGAGTGAGGGAGAGTGAGT17580.10664104369917035No Hit
GTGGGCTGTGGGGGGCTCCTGAGACCCACCTCTGCTCTGTGCTGCTGCGTAG17300.10494255153558857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC405.6134013E-946.00000431
CTATGAT750.046.00000441
CCTTACA206.3122547E-446.00000430
TACCCGT206.3122547E-446.00000439
ACGCCTA351.01992555E-746.00000439
CATATGT405.6134013E-946.00000445
AATTTCG405.6134013E-946.00000426
CGTATAT750.046.00000444
GATAACG351.01992555E-746.00000413
GATAAAC206.3122547E-446.00000414
AGATATA405.6134013E-946.00000434
CAACGTC1700.046.00000425
CCGATTG405.6134013E-946.00000430
CACGGAT351.01992555E-746.00000422
AGATAAT700.046.00000444
TAGACTA206.3122547E-446.00000423
ACTGACG405.6134013E-946.00000423
CTATCCA206.3122547E-446.00000417
ACGCAAC351.01992555E-746.00000436
CTATATC206.3122547E-446.00000438
Produced by FastQC (version 0.11.5)