##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528007_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1648521
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14346192738825	31.0	31.0	33.0	30.0	34.0
2	31.816047839244995	33.0	31.0	34.0	30.0	34.0
3	32.19733506579534	33.0	31.0	34.0	30.0	34.0
4	35.82163769827621	37.0	35.0	37.0	35.0	37.0
5	35.82078663238139	37.0	35.0	37.0	35.0	37.0
6	35.879097688170184	37.0	35.0	37.0	35.0	37.0
7	36.15299774767807	37.0	35.0	37.0	35.0	37.0
8	35.911595909302946	37.0	35.0	37.0	35.0	37.0
9	36.46184974289075	37.0	35.0	39.0	32.0	39.0
10	37.11677740228969	39.0	37.0	39.0	33.0	39.0
11	38.04966087784141	39.0	38.0	39.0	35.0	39.0
12	38.17926553559221	39.0	39.0	39.0	37.0	39.0
13	38.03865889485181	39.0	39.0	39.0	37.0	39.0
14	38.1920582146057	40.0	38.0	40.0	34.0	40.0
15	36.876634267928644	39.0	35.0	40.0	31.0	40.0
16	36.93941539112938	39.0	36.0	40.0	32.0	40.0
17	36.907805845360784	39.0	36.0	40.0	31.0	40.0
18	37.136620036990735	39.0	36.0	40.0	32.0	40.0
19	37.30217752761415	39.0	36.0	40.0	33.0	40.0
20	37.3233850220895	39.0	36.0	40.0	33.0	40.0
21	37.398048311183175	40.0	36.0	40.0	33.0	40.0
22	37.35347199095432	40.0	36.0	40.0	33.0	40.0
23	37.307698840354476	40.0	36.0	40.0	32.0	40.0
24	37.28901178692901	40.0	36.0	40.0	32.0	40.0
25	37.245280466551534	39.0	36.0	40.0	32.0	40.0
26	37.00994103199171	39.0	35.0	40.0	32.0	40.0
27	36.79363138231178	39.0	35.0	40.0	32.0	40.0
28	36.37145417013189	38.0	35.0	40.0	31.0	40.0
29	36.37948985787867	39.0	35.0	40.0	31.0	40.0
30	36.381913242233495	39.0	35.0	40.0	31.0	40.0
31	36.27794186425287	39.0	35.0	40.0	31.0	40.0
32	36.099366644404284	38.0	35.0	40.0	30.0	40.0
33	36.00609516044988	38.0	35.0	40.0	30.0	40.0
34	35.906724269815186	38.0	35.0	40.0	30.0	40.0
35	35.67425043417706	38.0	35.0	40.0	29.0	40.0
36	35.03126438789679	38.0	35.0	40.0	26.0	40.0
37	34.93674693861953	37.0	34.0	40.0	26.0	40.0
38	34.654142106773286	37.0	34.0	40.0	24.0	40.0
39	34.293575878014295	37.0	34.0	40.0	23.0	40.0
40	33.8174084527889	35.0	33.0	40.0	21.0	40.0
41	33.35892051117335	35.0	33.0	40.0	18.0	40.0
42	32.76160206633704	35.0	33.0	40.0	15.0	40.0
43	32.38781550250194	35.0	32.0	39.0	13.0	40.0
44	32.06303589702527	35.0	32.0	39.0	11.0	40.0
45	31.547251142084328	35.0	31.0	39.0	10.0	40.0
46	31.403868679865163	35.0	31.0	39.0	10.0	40.0
47	31.16406281751946	35.0	30.0	38.0	10.0	40.0
48	30.821883373035586	35.0	29.0	38.0	9.0	40.0
49	30.50811848923975	35.0	28.0	37.0	9.0	40.0
50	30.092976067638812	35.0	27.0	37.0	9.0	40.0
51	29.81178644372744	35.0	26.0	37.0	8.0	40.0
52	29.202667724584643	35.0	24.0	36.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	1.0
15	12.0
16	64.0
17	178.0
18	520.0
19	1156.0
20	2293.0
21	4161.0
22	6666.0
23	10263.0
24	14773.0
25	20710.0
26	27578.0
27	35716.0
28	44293.0
29	54318.0
30	61573.0
31	62724.0
32	64879.0
33	77788.0
34	114786.0
35	172396.0
36	164679.0
37	278776.0
38	320186.0
39	108029.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.12253832374596	21.97466698938018	32.52600361172227	12.376791075151605
2	20.338412431506786	22.186371905483764	32.726001063984015	24.74921459902543
3	20.84692885319629	21.236186860828585	34.546905984212515	23.369978301762607
4	23.95468422907564	20.34162743453071	38.69062025900792	17.01306807738573
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	99.28269036305876	0.4320236138939086	0.21376737087364975	0.07151865217367569
12	98.52813521938756	0.7405425833216562	0.36396260648181006	0.3673595908089736
13	92.52669514067458	4.348321920072598	1.6984315031473667	1.4265514361054545
14	43.30548412789403	35.481440636788975	9.548983604091182	11.664091631225808
15	26.288897745312312	39.54666030945314	19.73223271041133	14.432209234823215
16	32.190248107242795	29.16511224303482	21.98801228495118	16.65662736477121
17	20.775774163629094	28.236704294334135	26.250196388156414	24.737325153880356
18	21.98655643452525	30.752595811639644	27.22300777484788	20.037839978987225
19	25.432493732260614	30.44613929698196	26.43854703701075	17.682819933746675
20	26.826895138126844	29.04087967335569	26.043465627674745	18.088759560842718
21	27.773197914979548	28.03409844339259	20.940345922193288	23.25235771943457
22	29.602595296025953	28.432334195318106	23.062551220154308	18.902519288501633
23	26.0130140895991	29.622734560251278	20.777048032751782	23.587203317397837
24	26.149924690070677	30.894116605126655	24.01024918699853	18.94570951780414
25	31.001121611432307	28.493965196682357	20.37402010650759	20.13089308537774
26	28.251323459027823	30.373043473513533	22.640597238373065	18.735035829085586
27	34.03814692078536	32.851325521482586	17.36890218565611	15.74162537207594
28	32.50738085835728	31.60056802430785	18.343048101904678	17.549003015430195
29	31.833625413325034	34.05858948718276	15.91899648230141	18.188788617190806
30	31.22404870790242	36.61651868553692	15.524824979481608	16.63460762707906
31	26.603846720787907	38.620678778129005	15.810414304701	18.96506019638209
32	23.519688253895463	42.75001652996838	17.33748008062985	16.39281513550631
33	23.794722663526883	43.244399070439506	15.6173321419624	17.343546124071214
34	21.317835805549336	44.099529214368516	16.73518262733687	17.84745235274528
35	23.788231997044623	42.992961569794986	17.324438087230916	15.894368345929472
36	24.23184175391154	43.12611122333291	16.705944297949497	15.936102724806053
37	23.14565601530099	43.235663967883944	17.17327228467214	16.445407732142932
38	23.399277291584397	42.17137664609672	17.663226613431068	16.76611944888782
39	24.51178965873046	41.922486883697566	17.1383925348843	16.42733092268767
40	25.33567967893645	41.95057266483108	17.793889189158037	14.919858467074427
41	27.19316284111637	40.99335100978392	16.831208095013654	14.982278054086057
42	26.28756321575521	40.31589527825245	16.575342382656938	16.8211991233354
43	24.953882904737036	40.549437950744945	17.542633669816762	16.954045474701264
44	25.57911000223837	40.883373642192	16.56945832051882	16.968058035050813
45	25.041233930292666	39.04748559466334	17.922671291418187	17.988609183625808
46	26.367513668312387	39.64341370234289	15.482908619301785	18.50616401004294
47	25.60009851254549	40.6942950681247	15.854089817478819	17.851516601850992
48	24.30948710996099	41.01009328968209	16.188268150663536	18.49215144969339
49	24.79725766308103	40.117656978588684	16.040317351128678	19.044768007201608
50	24.145157993134454	41.09532120003324	16.708795338366937	18.05072546846537
51	23.181263690301794	40.472823822080514	17.804868727786907	18.54104375983078
52	23.77161103801529	38.714217167994825	18.05939991058652	19.454771883403367
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	79.0
12	156.0
13	628.0
14	2138.0
15	3176.0
16	4258.5
17	5341.0
18	5575.5
19	5810.0
20	5944.0
21	6078.0
22	7414.5
23	8751.0
24	11271.5
25	13792.0
26	17150.0
27	20508.0
28	25338.5
29	30169.0
30	37427.5
31	44686.0
32	50283.0
33	55880.0
34	60827.0
35	65774.0
36	68007.0
37	70240.0
38	69943.5
39	68929.0
40	68211.0
41	66741.0
42	65271.0
43	62250.5
44	59230.0
45	60646.0
46	62062.0
47	64127.5
48	66193.0
49	73496.0
50	80799.0
51	82622.0
52	84445.0
53	89036.0
54	93627.0
55	91774.0
56	89921.0
57	95574.0
58	101227.0
59	98789.0
60	96351.0
61	95591.0
62	94831.0
63	89529.0
64	71295.0
65	58363.0
66	53464.5
67	48566.0
68	38491.0
69	28416.0
70	26857.5
71	25299.0
72	20719.5
73	16140.0
74	13679.5
75	11219.0
76	8908.5
77	6598.0
78	5260.0
79	3922.0
80	2866.0
81	1810.0
82	966.0
83	122.0
84	251.0
85	380.0
86	273.0
87	166.0
88	89.5
89	7.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1648521.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.01903052069263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.74737654973934	26.494907752533496
2	6.178046542901247	3.9563012163082925
3	2.575680248491412	2.474123534639751
4	1.6298577260706846	2.087458571017758
5	1.2156284378099964	1.946162202603009
6	0.8726843065494972	1.6765503267802702
7	0.6943154230827462	1.5561914705871849
8	0.5593929623212817	1.4328976266900626
9	0.4551026611498392	1.3114751397664626
>10	2.522094047178958	13.670834851807365
>50	0.12396122139017106	2.8442975784395776
>100	0.37282381958028116	28.238438603911547
>500	0.04753601853247292	9.984255152658402
>1k	0.005500035202104306	2.3261059722568795
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCAAGCTGTGGGGAGCCAAGGTGGGTGGATCACTTGAGGCCAGGAGTTTGAG	1987	0.12053228317989277	No Hit
CACTGCTGTGGGGCTGGGATACATGAGGATCAATGCCCGTTTTCTCCCCGTG	1809	0.10973472585426573	No Hit
ACCTGCTGTGGGGGGGAGGGGCTGGAGAGATGGCTCATCGGTTAAGAGCTCT	1802	0.10931010281337028	No Hit
TAGGGCTGTGGGGGGTATATGTGTGTGTGTGAGTGAGTGAGGGAGAGTGAGT	1758	0.10664104369917035	No Hit
GTGGGCTGTGGGGGGCTCCTGAGACCCACCTCTGCTCTGTGCTGCTGCGTAG	1730	0.10494255153558857	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.009826990375008872	0.0	0.0	0.0	0.0
12	0.020017943356499552	0.0	0.0	0.0	0.0
13	0.06824298871533939	0.0	0.0	0.0	0.0
14	0.2516801423821717	0.0	0.0	0.0	0.0
15	0.38446583331361867	0.0	0.0	0.0	0.0
16	0.4754564849340712	0.0	0.0	0.0	0.0
17	0.5315673867666836	0.0	0.0	0.0	0.0
18	0.6008416028670548	0.0	0.0	0.0	0.0
19	0.6657482676896442	0.0	0.0	0.0	0.0
20	0.73393059597057	0.0	0.0	0.0	0.0
21	0.8061165129227956	0.0	0.0	0.0	0.0
22	0.8900705541512665	0.0	0.0	0.0	0.0
23	0.9897963083272825	0.0	0.0	0.0	0.0
24	1.1139075571375796	0.0	0.0	0.0	0.0
25	1.254154481501904	0.0	0.0	0.0	0.0
26	1.409202551863155	0.0	0.0	0.0	0.0
27	1.5714692139196285	0.0	0.0	0.0	0.0
28	1.7376788042129885	0.0	0.0	0.0	0.0
29	1.9158385000858345	0.0	0.0	0.0	0.0
30	2.117534444511171	0.0	0.0	0.0	0.0
31	2.3066130185784712	0.0	0.0	0.0	0.0
32	2.4719127023556267	0.0	0.0	0.0	0.0
33	2.6263541683727416	0.0	0.0	0.0	0.0
34	2.7732737405225656	0.0	0.0	0.0	0.0
35	2.909092453174694	0.0	0.0	0.0	0.0
36	3.0530396640382502	0.0	0.0	0.0	0.0
37	3.1957130057791194	0.0	0.0	0.0	0.0
38	3.3533088143857435	0.0	0.0	0.0	0.0
39	3.531893133299485	0.0	0.0	0.0	0.0
40	3.73340709642158	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	40	5.6134013E-9	46.000004	31
CTATGAT	75	0.0	46.000004	41
CCTTACA	20	6.3122547E-4	46.000004	30
TACCCGT	20	6.3122547E-4	46.000004	39
ACGCCTA	35	1.01992555E-7	46.000004	39
CATATGT	40	5.6134013E-9	46.000004	45
AATTTCG	40	5.6134013E-9	46.000004	26
CGTATAT	75	0.0	46.000004	44
GATAACG	35	1.01992555E-7	46.000004	13
GATAAAC	20	6.3122547E-4	46.000004	14
AGATATA	40	5.6134013E-9	46.000004	34
CAACGTC	170	0.0	46.000004	25
CCGATTG	40	5.6134013E-9	46.000004	30
CACGGAT	35	1.01992555E-7	46.000004	22
AGATAAT	70	0.0	46.000004	44
TAGACTA	20	6.3122547E-4	46.000004	23
ACTGACG	40	5.6134013E-9	46.000004	23
CTATCCA	20	6.3122547E-4	46.000004	17
ACGCAAC	35	1.01992555E-7	46.000004	36
CTATATC	20	6.3122547E-4	46.000004	38
>>END_MODULE