FastQCFastQC Report
Fri 17 Jun 2016
SRR1528006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1667126
Sequences flagged as poor quality0
Sequence length59
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGCTGTGGGGAGCCAAGGTGGGTGGATCACTTGAGGCCAGGAGTTTGAGACCAGCC20690.12410579644250046No Hit
CACTGCTGTGGGGCTGGGATACATGAGGATCAATGCCCGTTTTCTCCCCGTGTGCTTTC18970.1137886398508571No Hit
GTGGGCTGTGGGGGGCTCCTGAGACCCACCTCTGCTCTGTGCTGCTGCGTAGAGCTCTC17400.10437123528755476No Hit
ACCTGCTGTGGGGGGGAGGGGCTGGAGAGATGGCTCATCGGTTAAGAGCTCTGACTACT17370.10419128488188656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTT457.6397555E-1153.00000439
CGACGTA750.053.00000450
GTTTAGT457.6397555E-1153.00000434
GACCGAC750.053.00000415
TCTAGTC457.6397555E-1153.00000444
CGTAAGT457.6397555E-1153.00000426
CTACAAC457.6397555E-1153.00000447
TCGCGAT900.053.00000448
TAATCGA457.6397555E-1153.00000442
TATTTCG203.1386557E-453.051
ATTTACG550.053.023
TTAGGCG401.6025297E-953.016
CCTTATA307.012404E-753.033
ACTATCC251.4790223E-553.016
TACCCTA203.1386557E-453.023
TCGCACT150.00669679353.052
CAAACGA203.1386557E-453.044
GCCCACG150.00669679353.015
CTCAATT401.6025297E-953.051
ATAGGTG203.1386557E-453.028