##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528006_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1667126
Sequences flagged as poor quality	0
Sequence length	59
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.87305938483354	31.0	31.0	33.0	30.0	34.0
2	31.61222247148686	31.0	31.0	34.0	30.0	34.0
3	31.972124482492625	31.0	31.0	34.0	30.0	34.0
4	35.667217114963115	37.0	35.0	37.0	33.0	37.0
5	35.54850923085598	37.0	35.0	37.0	33.0	37.0
6	35.62756444323944	37.0	35.0	37.0	33.0	37.0
7	35.9040678389036	37.0	35.0	37.0	35.0	37.0
8	35.70769395954475	37.0	35.0	37.0	33.0	37.0
9	35.962968605852225	37.0	35.0	39.0	32.0	39.0
10	36.68048905721583	38.0	35.0	39.0	32.0	39.0
11	37.81339382866082	39.0	37.0	39.0	35.0	39.0
12	37.974213106867744	39.0	38.0	39.0	35.0	39.0
13	37.75807947329716	39.0	38.0	39.0	35.0	39.0
14	37.49783519661981	40.0	37.0	40.0	32.0	40.0
15	36.098773578001904	38.0	35.0	40.0	30.0	40.0
16	36.2395085914322	38.0	35.0	40.0	30.0	40.0
17	36.05762311906838	38.0	35.0	40.0	30.0	40.0
18	36.26453789335659	38.0	35.0	40.0	30.0	40.0
19	36.5392693773596	39.0	35.0	40.0	30.0	40.0
20	36.68413185326124	39.0	35.0	40.0	31.0	40.0
21	36.74226243247361	39.0	35.0	40.0	31.0	40.0
22	36.72224474934708	39.0	35.0	40.0	31.0	40.0
23	36.715560791445874	39.0	35.0	40.0	31.0	40.0
24	36.67201939145571	39.0	35.0	40.0	31.0	40.0
25	36.598177342324455	39.0	35.0	40.0	30.0	40.0
26	36.184998614381875	38.0	35.0	40.0	30.0	40.0
27	35.88831438055672	38.0	35.0	40.0	30.0	40.0
28	35.63308532168534	38.0	34.0	40.0	29.0	40.0
29	35.48194797513805	38.0	34.0	40.0	29.0	40.0
30	35.39585550222359	38.0	34.0	40.0	28.0	40.0
31	35.2381085772761	38.0	34.0	40.0	27.0	40.0
32	34.968074998530405	37.0	33.0	40.0	26.0	40.0
33	34.83609757150929	37.0	33.0	40.0	25.0	40.0
34	34.75448706336534	37.0	33.0	40.0	25.0	40.0
35	34.592685255943465	37.0	33.0	40.0	24.0	40.0
36	34.231237470953005	37.0	33.0	40.0	23.0	40.0
37	33.932843108439315	36.0	33.0	40.0	22.0	40.0
38	33.545558644037705	35.0	33.0	40.0	21.0	40.0
39	33.1517059898292	35.0	32.0	39.0	20.0	40.0
40	32.64201805982271	35.0	31.0	39.0	18.0	40.0
41	32.17511033959041	35.0	31.0	39.0	15.0	40.0
42	31.748727450714583	35.0	30.0	39.0	14.0	40.0
43	31.3106819760474	35.0	30.0	38.0	10.0	40.0
44	30.972332625128516	35.0	29.0	38.0	10.0	40.0
45	30.642487730381507	35.0	28.0	38.0	10.0	40.0
46	30.310672378692434	35.0	26.0	38.0	9.0	40.0
47	30.022524992112174	35.0	26.0	37.0	9.0	40.0
48	29.655257011167723	34.0	24.0	37.0	9.0	40.0
49	29.382450396670677	34.0	24.0	36.0	9.0	40.0
50	28.97605040050962	34.0	23.0	36.0	8.0	39.0
51	28.615303222431898	34.0	23.0	36.0	8.0	39.0
52	28.273447237941223	33.0	22.0	35.0	8.0	39.0
53	27.850936881795377	33.0	20.0	35.0	8.0	39.0
54	27.417621703458526	33.0	20.0	35.0	8.0	38.0
55	27.02751141785324	33.0	18.0	35.0	8.0	38.0
56	26.490839324682117	33.0	15.0	35.0	8.0	38.0
57	26.051840112864895	32.0	13.0	35.0	8.0	37.0
58	25.61600143000589	32.0	10.0	35.0	8.0	37.0
59	24.96163037466874	31.0	10.0	35.0	8.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	17.0
15	72.0
16	309.0
17	966.0
18	2375.0
19	4969.0
20	8563.0
21	13428.0
22	19524.0
23	26949.0
24	35360.0
25	44478.0
26	54677.0
27	62958.0
28	63719.0
29	60898.0
30	65635.0
31	77196.0
32	91060.0
33	109249.0
34	145452.0
35	162959.0
36	167766.0
37	211397.0
38	202948.0
39	34197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.15166340156653	22.35098006989274	32.062123678714144	12.435232849826587
2	19.8211173000721	22.65011762758184	32.56076625282072	24.967998819525338
3	20.943228046350427	21.46844329702734	34.379764936783424	23.20856371983881
4	23.734738706012624	20.428809820013605	38.63043345254048	17.20601802143329
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	99.27354021231749	0.4392589402360709	0.21480080089927217	0.0724000465471716
12	98.54486103629839	0.7490135718595955	0.3464045309112809	0.35972086093072747
13	92.54813373434281	4.3635574035795734	1.6524845752510608	1.4358242868265507
14	43.07472860479652	35.7654430438971	9.490404444535086	11.669423906771295
15	26.212175924315257	39.80245044465745	19.657782315193934	14.327591315833358
16	32.3775767398505	29.178598378286946	21.85983542935567	16.583989452506888
17	20.685898966244903	28.26169107793892	26.12783916752543	24.924570788290747
18	21.905842749738174	30.923037610834452	27.091113689067292	20.080005950360082
19	25.470960203367955	30.48203915001026	26.38678780128197	17.660212845339824
20	26.75484636434199	29.12437332271226	26.117462027465233	18.00331828548052
21	27.780443709713605	28.05570784691739	21.012149051721345	23.15169939164766
22	29.628894276737334	28.468694027925906	22.99364295200243	18.908768743334335
23	25.910878961758137	29.79720788950565	20.879825520086666	23.412087628649545
24	26.10816458983904	31.004735095007817	24.038554974249095	18.848545340904046
25	30.937673577162133	28.544033264432322	20.463420281370453	20.05487287703509
26	28.039752244281473	30.534524684996818	22.694865295124664	18.730857775597045
27	33.9291091375217	33.060728463235534	17.38632832791283	15.623834071329942
28	32.392692573926624	31.83274689495575	18.245111647230022	17.5294488838876
29	31.795497160982432	34.09970212209515	15.925490934698397	18.179309782224017
30	31.24676839063154	36.685109583798706	15.535658372552524	16.532463653017228
31	26.434234724909818	38.84043557595527	15.776851899616466	18.948477799518454
32	23.426243727228776	43.189297029738604	17.23109111128973	16.153368131742894
33	23.743856193233146	43.55903512991819	15.451621533105476	17.245487143743183
34	21.064154718959454	44.47906157063113	16.697478175014965	17.759305535394446
35	23.64866242863467	43.47853731511596	17.194561178939086	15.678239077310293
36	23.780985960269348	44.02324719307359	16.766099263043106	15.429667583613957
37	22.96341128385017	43.74018520495751	17.036564722762407	16.25983878842991
38	23.162796333330533	42.68129703453728	17.49543825721631	16.660468374915872
39	24.155522737933424	42.62131356598122	16.97094280816207	16.25222088792329
40	24.99193222347921	42.65190513494481	17.606827558324927	14.749335083251056
41	26.76324404993984	41.73697728905913	16.605883418529853	14.893895242471174
42	25.573951818878715	41.3109147119054	16.373987329092103	16.74114614012378
43	24.59976030605965	41.285301770831964	17.360715386839388	16.754222536269005
44	24.890200260808122	41.79432148499874	16.453045540648997	16.86243271354415
45	24.32425623498164	40.20098060974395	17.712218512577934	17.762544642696472
46	25.92689454786261	40.37853167666991	15.266632516078568	18.427941259388913
47	25.167623802879923	41.495003976903966	15.55725242123271	17.780119798983403
48	23.713384591206662	41.857004209639825	15.965319957819624	18.46429124133389
49	24.408652975240024	41.00709844366893	15.708110844651213	18.876137736439837
50	23.521677425701476	42.12938914035292	16.353952850594375	17.99498058335123
51	22.74447162362053	41.32657039719853	17.507914818675975	18.421043160504965
52	23.129685458687586	39.66712773959497	17.733032776166887	19.47015402555056
53	22.306172418881356	41.34924414831273	17.52123114869542	18.8233522841105
54	22.003855737358784	39.501153482100335	18.74387418827371	19.75111659226717
55	23.51220003766962	37.75875368748373	18.30305567785518	20.42599059699147
56	23.92320676421578	37.049089270996916	18.92166518907389	20.106038775713415
57	25.27385452569272	34.5565962020867	19.349287336410086	20.820261935810493
58	24.2032095954355	34.12465524501447	19.375680062574755	22.296455096975272
59	24.15714229158444	33.34780934374486	20.492872164431482	22.002176200239216
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	52.5
10	105.0
11	420.5
12	736.0
13	1416.0
14	2816.0
15	3536.0
16	3403.0
17	3270.0
18	2980.0
19	2858.5
20	3027.0
21	3720.0
22	4413.0
23	5007.5
24	5602.0
25	7418.0
26	12832.0
27	16430.0
28	21517.5
29	26605.0
30	34019.5
31	49493.0
32	57552.0
33	63369.5
34	69187.0
35	71007.0
36	72376.5
37	71926.0
38	70486.0
39	69046.0
40	67482.0
41	65918.0
42	64866.5
43	64331.0
44	64847.0
45	63834.0
46	62821.0
47	65997.5
48	68558.0
49	67942.0
50	63286.0
51	58630.0
52	62002.5
53	69076.5
54	72778.0
55	78646.0
56	84514.0
57	83314.5
58	84774.5
59	87434.0
60	84639.5
61	81845.0
62	77933.5
63	74022.0
64	62127.0
65	50301.0
66	50370.0
67	41031.5
68	31693.0
69	27014.5
70	21037.0
71	19738.0
72	15796.0
73	11854.0
74	9748.5
75	6462.0
76	5281.0
77	4536.5
78	3792.0
79	2738.0
80	1684.0
81	1143.5
82	709.5
83	816.0
84	457.5
85	99.0
86	62.5
87	17.0
88	8.0
89	5.0
90	2.0
91	2.5
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1667126.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.49595253995337
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.26681748695606	37.042116218314085
2	4.533032874569733	3.7620503405038805
3	1.793941902304376	2.2332398411236816
4	0.9856051751032814	1.635945022768728
5	0.6213057960949653	1.2890837913777315
6	0.4335317759276469	1.0793888399073204
7	0.3367421377340269	0.9781405035929536
8	0.24675858846764293	0.8191586140703357
9	0.18304161172646974	0.6835937429734346
>10	1.1955514962781628	8.767842362356026
>50	0.08852970533636184	2.6582618426038906
>100	0.27704580348002883	27.348472213746906
>500	0.03350045136500056	9.224517548351544
>1k	0.004595194656261139	2.4781891183095976
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCAAGCTGTGGGGAGCCAAGGTGGGTGGATCACTTGAGGCCAGGAGTTTGAGACCAGCC	2069	0.12410579644250046	No Hit
CACTGCTGTGGGGCTGGGATACATGAGGATCAATGCCCGTTTTCTCCCCGTGTGCTTTC	1897	0.1137886398508571	No Hit
GTGGGCTGTGGGGGGCTCCTGAGACCCACCTCTGCTCTGTGCTGCTGCGTAGAGCTCTC	1740	0.10437123528755476	No Hit
ACCTGCTGTGGGGGGGAGGGGCTGGAGAGATGGCTCATCGGTTAAGAGCTCTGACTACT	1737	0.10419128488188656	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.01007722271741908	0.0	0.0	0.0	0.0
12	0.02075428012039882	0.0	0.0	0.0	0.0
13	0.06568189806889221	0.0	0.0	0.0	0.0
14	0.23537513061400278	0.0	0.0	0.0	0.0
15	0.3575614560627091	0.0	0.0	0.0	0.0
16	0.4396788245159634	0.0	0.0	0.0	0.0
17	0.4927042107195257	0.0	0.0	0.0	0.0
18	0.5581461749141936	0.0	0.0	0.0	0.0
19	0.6146506022940078	0.0	0.0	0.0	0.0
20	0.6723546990449432	0.0	0.0	0.0	0.0
21	0.7317983163840046	0.0	0.0	0.0	0.0
22	0.8106165940666752	0.0	0.0	0.0	0.0
23	0.9028111852373486	0.0	0.0	0.0	0.0
24	1.0182793622077755	0.0	0.0	0.0	0.0
25	1.1377664315714588	0.0	0.0	0.0	0.0
26	1.277827830649873	0.0	0.0	0.0	0.0
27	1.4155498744546002	0.0	0.0	0.0	0.0
28	1.557170843715472	0.0	0.0	0.0	0.0
29	1.718886274942626	0.0	0.0	0.0	0.0
30	1.8920585486639883	0.0	0.0	0.0	0.0
31	2.061991715083323	0.0	0.0	0.0	0.0
32	2.2164491466151928	0.0	0.0	0.0	0.0
33	2.352791570643131	0.0	0.0	0.0	0.0
34	2.4826557800670135	0.0	0.0	0.0	0.0
35	2.605801841012617	0.0	0.0	0.0	0.0
36	2.7301475713293417	0.0	0.0	0.0	0.0
37	2.8626510533696914	0.0	0.0	0.0	0.0
38	3.0039121218192264	0.0	0.0	0.0	0.0
39	3.163528131646918	0.0	0.0	0.0	0.0
40	3.3440783720006766	0.0	0.0	0.0	0.0
41	3.5293673063703643	0.0	0.0	0.0	0.0
42	3.7223341247152284	0.0	0.0	0.0	0.0
43	3.9170404636482186	0.0	0.0	0.0	0.0
44	4.109467430776078	0.0	0.0	0.0	0.0
45	4.295656117174107	0.0	0.0	0.0	0.0
46	4.469248275175362	0.0	0.0	0.0	5.998346855606595E-5
47	4.632103392305081	0.0	0.0	0.0	5.998346855606595E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTT	45	7.6397555E-11	53.000004	39
CGACGTA	75	0.0	53.000004	50
GTTTAGT	45	7.6397555E-11	53.000004	34
GACCGAC	75	0.0	53.000004	15
TCTAGTC	45	7.6397555E-11	53.000004	44
CGTAAGT	45	7.6397555E-11	53.000004	26
CTACAAC	45	7.6397555E-11	53.000004	47
TCGCGAT	90	0.0	53.000004	48
TAATCGA	45	7.6397555E-11	53.000004	42
TATTTCG	20	3.1386557E-4	53.0	51
ATTTACG	55	0.0	53.0	23
TTAGGCG	40	1.6025297E-9	53.0	16
CCTTATA	30	7.012404E-7	53.0	33
ACTATCC	25	1.4790223E-5	53.0	16
TACCCTA	20	3.1386557E-4	53.0	23
TCGCACT	15	0.006696793	53.0	52
CAAACGA	20	3.1386557E-4	53.0	44
GCCCACG	15	0.006696793	53.0	15
CTCAATT	40	1.6025297E-9	53.0	51
ATAGGTG	20	3.1386557E-4	53.0	28
>>END_MODULE