FastQCFastQC Report
Fri 17 Jun 2016
SRR1528005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3692599
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG253.4186352E-546.034
AGGTACT600.046.015
GATACAC650.046.035
CGAACTA650.046.015
TCGCAAT301.8625306E-646.039
ATTAGTA405.6170393E-946.019
CGTATAC206.313279E-446.016
TTTACTA206.313279E-446.026
CGACGTA206.313279E-446.020
CCTAGTA253.4186352E-546.040
AGATACG206.313279E-446.023
ACGTTAT206.313279E-446.045
CTATATA453.110472E-1046.018
CGCATTC650.046.039
CACATAA253.4186352E-546.021
GCTATAT551.8189894E-1246.021
GCACGTA301.8625306E-646.015
TAAACGA301.8625306E-646.039
ATTCGTC501.6370905E-1146.045
GCGTTTA301.8625306E-646.035
Produced by FastQC (version 0.11.5)