##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528005_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3692599
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18588885497721	31.0	31.0	33.0	30.0	33.0
2	31.928364005948115	31.0	31.0	34.0	30.0	34.0
3	32.328201356280495	33.0	31.0	34.0	30.0	34.0
4	35.95552752952595	37.0	35.0	37.0	35.0	37.0
5	36.0305836079141	37.0	35.0	37.0	35.0	37.0
6	36.016560422618326	37.0	35.0	37.0	35.0	37.0
7	35.85600440231934	37.0	35.0	37.0	35.0	37.0
8	32.703704626470405	35.0	32.0	35.0	28.0	35.0
9	36.816985272432774	37.0	37.0	39.0	34.0	39.0
10	37.87839432334786	39.0	37.0	39.0	35.0	39.0
11	37.891792745434856	39.0	37.0	39.0	35.0	39.0
12	38.0399358283962	39.0	38.0	39.0	35.0	39.0
13	38.05686455529019	39.0	39.0	39.0	37.0	39.0
14	38.682041835574346	40.0	39.0	40.0	36.0	40.0
15	38.11119485218948	40.0	38.0	40.0	34.0	40.0
16	37.660231452156054	40.0	37.0	40.0	33.0	40.0
17	37.69029726758849	40.0	37.0	40.0	33.0	40.0
18	37.69347497521394	40.0	37.0	40.0	33.0	40.0
19	37.806326655019944	40.0	37.0	40.0	33.0	40.0
20	37.84400797378757	40.0	37.0	40.0	33.0	40.0
21	37.66495685017517	40.0	37.0	40.0	33.0	40.0
22	37.6758854671195	40.0	37.0	40.0	33.0	40.0
23	37.59859139863278	40.0	37.0	40.0	33.0	40.0
24	37.6001802524455	40.0	37.0	40.0	33.0	40.0
25	37.65372275733162	40.0	37.0	40.0	33.0	40.0
26	37.61563522061291	40.0	37.0	40.0	33.0	40.0
27	37.54645630354122	40.0	37.0	40.0	33.0	40.0
28	37.526464422484004	40.0	37.0	40.0	33.0	40.0
29	37.52111805262364	40.0	36.0	40.0	33.0	40.0
30	37.45998143854776	40.0	36.0	40.0	33.0	40.0
31	37.393333530123364	40.0	36.0	40.0	33.0	40.0
32	37.29465750275077	40.0	35.0	40.0	33.0	40.0
33	37.18859128759987	40.0	35.0	40.0	33.0	40.0
34	37.07313737559914	39.0	35.0	40.0	33.0	40.0
35	36.93787356818328	39.0	35.0	40.0	32.0	40.0
36	36.80403450252789	39.0	35.0	40.0	32.0	40.0
37	36.62488480335937	39.0	35.0	40.0	32.0	40.0
38	36.392357252980894	38.0	35.0	40.0	31.0	40.0
39	36.21086611354225	38.0	35.0	40.0	31.0	40.0
40	35.998366733024625	38.0	35.0	40.0	31.0	40.0
41	35.809516007559985	37.0	35.0	40.0	30.0	40.0
42	35.588531546479864	37.0	35.0	40.0	30.0	40.0
43	35.32427133300962	37.0	35.0	40.0	30.0	40.0
44	35.0965428415054	36.0	34.0	40.0	29.0	40.0
45	34.90391699721524	36.0	34.0	40.0	29.0	40.0
46	34.71834824198349	35.0	34.0	39.0	28.0	40.0
47	34.53043967135343	35.0	34.0	39.0	28.0	40.0
48	34.33589783239393	35.0	34.0	39.0	27.0	40.0
49	34.150539227248885	35.0	34.0	39.0	27.0	40.0
50	33.951218098688756	35.0	33.0	38.0	27.0	40.0
51	33.72537554172549	35.0	33.0	38.0	26.0	40.0
52	33.10425664958475	35.0	33.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	7.0
14	12.0
15	22.0
16	62.0
17	157.0
18	396.0
19	864.0
20	1692.0
21	3073.0
22	5108.0
23	8187.0
24	12554.0
25	18166.0
26	25409.0
27	34091.0
28	45538.0
29	59063.0
30	74400.0
31	91771.0
32	111367.0
33	143560.0
34	216844.0
35	331421.0
36	434310.0
37	773183.0
38	1049178.0
39	252160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.15223071879725	18.975577905968127	34.95584004653633	18.916351328698298
2	16.92520633840826	23.7408936090813	35.347136258228964	23.98676379428148
3	17.565487072926143	25.338711297923222	37.853419772902505	19.242381856248134
4	19.54994300762146	24.30599694144964	32.87494797025076	23.269112080678134
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.44667698821345	0.16817423175384058	0.3019553436482001	0.08319343638450857
12	99.17979179434323	0.4540162633418901	0.18583117202815685	0.1803607702867276
13	97.7223901105969	1.2883879348935532	0.414342310118158	0.5748796443913894
14	58.447586645611935	24.523296464089388	7.079972669656251	9.949144220642426
15	32.273609996644645	25.172080694383546	19.851790026482703	22.70251928248911
16	27.90836481296778	18.58896674131147	28.104622245740735	25.398046199980012
17	24.066869974237655	18.262854970171418	30.14045121065136	27.529823844939568
18	24.595603259384514	19.685511478500644	29.409827603809674	26.309057658305168
19	26.033208588314082	18.141179153219724	29.003745058697138	26.821867199769052
20	30.27637173708816	17.290342114050294	25.01162460370054	27.421661545161008
21	31.540630325686596	19.620516606325246	20.12498513919329	28.71386792879487
22	31.37489340163933	19.80772891938713	21.362487505412854	27.45489017356068
23	29.366416445435856	20.082223929541225	22.578081183469962	27.973278441552957
24	30.3174809937391	20.67622289883088	22.18250614269245	26.82378996473757
25	33.1103648135094	18.97733818375621	22.539084260164724	25.373212742569663
26	31.927945601458486	18.981454525660652	21.731468810991935	27.35913106188893
27	32.398102258057264	21.054818029252566	20.81823669453412	25.728843018156045
28	31.767976972316784	20.69409648867911	19.95261332194479	27.585313217059316
29	30.5520583198988	21.013898340978805	20.373400956887007	28.060642382235386
30	31.38404684613737	20.88496476330086	19.353496006471325	28.37749238409045
31	29.643971630821543	20.611173864262	20.605540975340134	29.13931352957632
32	31.476935350954705	22.869637347570098	18.508725155371597	27.1447021461036
33	30.385048579604774	23.333619491312216	20.177062280523828	26.104269648559185
34	30.04466501778287	23.66782854027746	19.89392836860975	26.39357807332992
35	31.48094336807219	23.439534051761374	19.740269658308414	25.33925292185802
36	33.07288443722159	22.369826780541295	19.28281408298058	25.274474699256537
37	32.35114346291054	23.220582576120506	19.848404876890232	24.57986908407872
38	30.958520001765695	25.034020753404306	21.461523441890115	22.54593580293988
39	31.975012721392172	24.31452751842266	21.49236892497669	22.218090835208482
40	32.49150530561266	24.115941102730083	19.0755075219378	24.31704606971946
41	31.93720737074348	25.654640539089137	18.020180366186526	24.38797172398086
42	32.27109144534784	25.319402404647782	18.401862753036546	24.007643396967826
43	31.839850468464082	23.917327605840764	19.19209207390242	25.050729851792735
44	29.75427334514254	24.32443923642941	20.304100174429987	25.617187243998064
45	28.170998258949865	24.33080331766325	20.604024428322706	26.89417399506418
46	28.9219598445431	22.45748861438786	21.812441589243782	26.80810995182526
47	28.267515644130327	21.689682524422498	22.806456915576266	27.23634491587091
48	29.386185719055874	21.233310196964254	22.433440511682964	26.94706357229691
49	29.43030098854492	21.659947370402254	21.37819459952191	27.531557041530913
50	28.87735711351273	23.104322998516764	20.7083953605577	27.309924527412804
51	28.580818009212482	23.922012652876738	21.42100997156745	26.076159366343326
52	30.155562518432138	23.103185588253695	21.24224699188837	25.4990049014258
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	19.5
10	39.0
11	333.5
12	628.0
13	1115.5
14	2382.0
15	3161.0
16	3139.5
17	3118.0
18	3428.5
19	3739.0
20	4539.5
21	5340.0
22	6940.0
23	8540.0
24	12466.0
25	16392.0
26	20988.0
27	25584.0
28	29155.0
29	32726.0
30	33159.5
31	33593.0
32	32275.0
33	30957.0
34	32162.5
35	33368.0
36	33320.0
37	33272.0
38	35261.0
39	39501.5
40	41753.0
41	47983.5
42	54214.0
43	57540.5
44	60867.0
45	77027.0
46	93187.0
47	97191.0
48	101195.0
49	120419.0
50	139643.0
51	162584.5
52	185526.0
53	212230.0
54	238934.0
55	257248.0
56	275562.0
57	303772.5
58	331983.0
59	343109.0
60	354235.0
61	359934.0
62	365633.0
63	341770.5
64	290114.0
65	262320.0
66	223190.0
67	184060.0
68	164440.5
69	144821.0
70	128679.0
71	112537.0
72	93438.0
73	74339.0
74	58656.0
75	42973.0
76	34094.0
77	25215.0
78	17628.0
79	10041.0
80	7108.0
81	4175.0
82	3100.5
83	2026.0
84	1072.0
85	118.0
86	67.0
87	16.0
88	11.5
89	4.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3692599.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.995449721239433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.59224455676194	7.655739295039887
2	7.898470242910201	1.5789752437542943
3	3.6115560590379134	1.082973810106533
4	2.107729356465494	0.8427081123412475
5	1.3707652828270835	0.6850707732059332
6	0.9164538742426067	0.5496221173096237
7	0.6859381212786982	0.47993820021858663
8	0.4845924831013078	0.38749758401037543
9	0.3890217961731762	0.3499603023703714
>10	2.7770172446913577	5.554907844835919
>50	0.5357019489492157	3.9249967986194725
>100	2.3559808340961323	57.70981680404823
>500	0.252991230489955	16.358277767219867
>1k	0.021536968974899186	2.8395153469196552
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0010832478695899555	0.0	0.0	0.0	0.0
12	0.004008017117482835	0.0	0.0	0.0	0.0
13	0.010399179548063573	0.0	0.0	0.0	0.0
14	0.061230585828572236	0.0	0.0	0.0	0.0
15	0.1193197528353336	0.0	0.0	0.0	0.0
16	0.15122140259475778	0.0	0.0	0.0	0.0
17	0.17074694544411673	0.0	0.0	0.0	0.0
18	0.19319725754136857	0.0	0.0	0.0	0.0
19	0.21407686022771494	0.0	0.0	0.0	0.0
20	0.23972275354025716	0.0	0.0	0.0	0.0
21	0.26666854429630726	0.0	0.0	0.0	0.0
22	0.2950767196763039	0.0	0.0	0.0	0.0
23	0.322022510432354	0.0	0.0	0.0	0.0
24	0.35262426274827025	0.0	0.0	0.0	0.0
25	0.3860695407218601	0.0	0.0	0.0	0.0
26	0.42910156234132113	0.0	0.0	0.0	0.0
27	0.4735959685847285	0.0	0.0	0.0	0.0
28	0.517548750893341	0.0	0.0	0.0	0.0
29	0.5716569819793592	0.0	0.0	0.0	0.0
30	0.6347561703829742	0.0	0.0	0.0	0.0
31	0.6879978031733205	0.0	0.0	0.0	0.0
32	0.7292695470046978	0.0	0.0	0.0	0.0
33	0.7699184233110609	0.0	0.0	0.0	0.0
34	0.8092132397804365	0.0	0.0	0.0	0.0
35	0.8450145818703845	0.0	0.0	0.0	0.0
36	0.881628359862525	0.0	0.0	0.0	0.0
37	0.9195961976916529	0.0	0.0	0.0	0.0
38	0.9600013432273583	0.0	0.0	0.0	0.0
39	1.0013001682554754	0.0	0.0	0.0	0.0
40	1.046200792449979	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	25	3.4186352E-5	46.0	34
AGGTACT	60	0.0	46.0	15
GATACAC	65	0.0	46.0	35
CGAACTA	65	0.0	46.0	15
TCGCAAT	30	1.8625306E-6	46.0	39
ATTAGTA	40	5.6170393E-9	46.0	19
CGTATAC	20	6.313279E-4	46.0	16
TTTACTA	20	6.313279E-4	46.0	26
CGACGTA	20	6.313279E-4	46.0	20
CCTAGTA	25	3.4186352E-5	46.0	40
AGATACG	20	6.313279E-4	46.0	23
ACGTTAT	20	6.313279E-4	46.0	45
CTATATA	45	3.110472E-10	46.0	18
CGCATTC	65	0.0	46.0	39
CACATAA	25	3.4186352E-5	46.0	21
GCTATAT	55	1.8189894E-12	46.0	21
GCACGTA	30	1.8625306E-6	46.0	15
TAAACGA	30	1.8625306E-6	46.0	39
ATTCGTC	50	1.6370905E-11	46.0	45
GCGTTTA	30	1.8625306E-6	46.0	35
>>END_MODULE