FastQCFastQC Report
Fri 17 Jun 2016
SRR1528004_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528004_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3662413
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA700.046.00000415
CGTATGT351.0203985E-746.00000431
CAATTTA351.0203985E-746.00000445
ATACGTT351.0203985E-746.00000433
TCGAATA351.0203985E-746.00000415
GCGTAAT351.0203985E-746.00000415
ACGCGTA351.0203985E-746.00000428
TTAGGTA253.4186298E-546.027
TAATACT405.6170393E-946.034
TAATACA206.3132716E-446.032
CGAGTTA650.046.025
CGTATAG253.4186298E-546.045
TTTACTA206.3132716E-446.026
AGATATA453.110472E-1046.030
CGACGAA453.110472E-1046.016
ACGTTAT206.3132716E-446.045
ATAATAC206.3132716E-446.031
CATGACG253.4186298E-546.024
ACTCTAT301.8625269E-646.024
ACCGAAT405.6170393E-946.027
Produced by FastQC (version 0.11.5)