##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528004_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3662413
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14599718819259	31.0	31.0	33.0	30.0	33.0
2	31.775010082150757	31.0	31.0	34.0	30.0	34.0
3	32.25781308661803	33.0	31.0	34.0	30.0	34.0
4	35.92228402422119	37.0	35.0	37.0	35.0	37.0
5	36.01450327966835	37.0	35.0	37.0	35.0	37.0
6	36.00321591256912	37.0	35.0	37.0	35.0	37.0
7	35.848906991101224	37.0	35.0	37.0	35.0	37.0
8	32.68345623500135	35.0	32.0	35.0	28.0	35.0
9	36.83838551250228	37.0	37.0	39.0	34.0	39.0
10	37.88174654251173	39.0	37.0	39.0	35.0	39.0
11	37.87724186212751	39.0	37.0	39.0	35.0	39.0
12	38.01559818622313	39.0	38.0	39.0	35.0	39.0
13	38.05393493306189	39.0	39.0	39.0	37.0	39.0
14	38.70076449597574	40.0	39.0	40.0	36.0	40.0
15	38.12159797379487	40.0	38.0	40.0	34.0	40.0
16	37.68225292996721	40.0	37.0	40.0	33.0	40.0
17	37.72954825138508	40.0	37.0	40.0	33.0	40.0
18	37.75639475941135	40.0	37.0	40.0	33.0	40.0
19	37.86585811048617	40.0	37.0	40.0	33.0	40.0
20	37.88376078831087	40.0	37.0	40.0	34.0	40.0
21	37.88685901890366	40.0	37.0	40.0	34.0	40.0
22	37.79544360507676	40.0	37.0	40.0	33.0	40.0
23	37.69336063409561	40.0	37.0	40.0	33.0	40.0
24	37.69375436358488	40.0	37.0	40.0	33.0	40.0
25	37.616053405227646	40.0	37.0	40.0	33.0	40.0
26	37.59085471791412	40.0	37.0	40.0	33.0	40.0
27	37.51181802816886	40.0	37.0	40.0	33.0	40.0
28	37.3443937098301	40.0	36.0	40.0	33.0	40.0
29	37.437109086277275	40.0	36.0	40.0	33.0	40.0
30	37.441792064412176	40.0	36.0	40.0	33.0	40.0
31	37.41173428556528	40.0	36.0	40.0	33.0	40.0
32	37.31582620529143	40.0	36.0	40.0	33.0	40.0
33	37.201282870064084	40.0	35.0	40.0	33.0	40.0
34	37.11116987625372	39.0	35.0	40.0	33.0	40.0
35	36.90799808759962	39.0	35.0	40.0	32.0	40.0
36	36.4003300556218	39.0	35.0	40.0	31.0	40.0
37	36.43499463331962	39.0	35.0	40.0	31.0	40.0
38	36.31688070132997	38.0	35.0	40.0	31.0	40.0
39	36.175962951201846	38.0	35.0	40.0	31.0	40.0
40	35.976354387121276	38.0	35.0	40.0	31.0	40.0
41	35.81928635574415	37.0	35.0	40.0	30.0	40.0
42	35.44964726807162	37.0	35.0	40.0	30.0	40.0
43	35.192587509928565	37.0	34.0	40.0	29.0	40.0
44	34.971868819819065	36.0	34.0	40.0	28.0	40.0
45	34.55006876613861	36.0	34.0	40.0	27.0	40.0
46	34.51617854130596	35.0	34.0	39.0	27.0	40.0
47	34.40037756528278	35.0	34.0	39.0	27.0	40.0
48	34.206023187445	35.0	34.0	39.0	27.0	40.0
49	34.033206522584976	35.0	34.0	39.0	27.0	40.0
50	33.767126208868305	35.0	33.0	38.0	26.0	40.0
51	33.63467746537597	35.0	33.0	38.0	26.0	40.0
52	33.138579401067	35.0	33.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	7.0
14	9.0
15	26.0
16	86.0
17	141.0
18	376.0
19	878.0
20	1717.0
21	3134.0
22	5168.0
23	8429.0
24	12642.0
25	18348.0
26	25702.0
27	34897.0
28	46136.0
29	59615.0
30	74971.0
31	92449.0
32	113265.0
33	146967.0
34	218853.0
35	332871.0
36	436954.0
37	766859.0
38	1010480.0
39	251431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.13604391421721	18.976095814426174	34.95921950910506	18.928640762251554
2	17.103505257326248	23.794176134695896	35.14240474790801	23.95991386006985
3	17.604732180668865	25.31180945458636	37.866974587519216	19.216483777225562
4	19.611988052685483	24.293628271852462	32.83125087203437	23.263132803427684
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.4405873941579	0.17046138706912628	0.3042529610942294	0.08469825767874896
12	99.18925036581074	0.4480652509697841	0.18782698728952743	0.1748573959299511
13	97.71658739743442	1.2922354742624602	0.4137709209747781	0.5774062073283379
14	58.458344266471315	24.4687860162139	7.103677275064281	9.969192442250506
15	32.33297282420087	25.10970226459987	19.886861476299913	22.67046343489934
16	27.875228708504473	18.629903290535502	28.04372417856752	25.451143822392503
17	24.056844490230894	18.235027016341412	30.20705201734485	27.501076476082847
18	24.535217628377794	19.6890410775628	29.441573083101225	26.334168210958186
19	26.02983333665537	18.113522423604326	29.011801782049158	26.844842457691144
20	30.307777959503746	17.304329140378215	24.97462738364024	27.413265516477797
21	31.302040485330302	19.63858254107333	20.143850516039564	28.915526457556805
22	31.403831299200828	19.768879151532065	21.401327485458356	27.42596206380875
23	29.380520438301193	20.043042660672075	22.552399196922902	28.024037704103826
24	30.275449546514828	20.672245320230132	22.27231063236178	26.779994500893263
25	33.14394089361304	18.952149853115966	22.5453819653873	25.358527287883696
26	31.971872096347408	18.968887452070536	21.65681478304058	27.40242566854148
27	32.41496794599626	21.05456156910758	20.81368212705667	25.716788357839487
28	31.85372594516238	20.690593878953575	19.961921279768283	27.493758896115754
29	30.57795502582587	21.04604259541455	20.31275555214554	28.063246826614037
30	31.354683374048747	20.935678199045274	19.35382492362276	28.355813503283215
31	29.60053931656533	20.629022450499164	20.650292580328873	29.120145652606627
32	31.462918026994767	22.890618835177793	18.504084602146182	27.142378535681257
33	30.33396288184866	23.33499799176117	20.263307278561975	26.067731847828195
34	30.06607938536697	23.686105308167047	19.834054761164293	26.41376054530169
35	31.484870766896034	23.450604833480003	19.7182021798197	25.346322219804268
36	33.18880202751574	22.27029010654997	19.359968414266767	25.180939451667523
37	32.31656287808065	23.22100210981121	19.83380902153853	24.628625990569606
38	30.942769152468603	25.067216613746186	21.41727325672992	22.572740977055293
39	31.961086857216813	24.334366440922967	21.46969771022547	22.234848991634752
40	32.445275833173376	24.180233086765472	19.02488878234104	24.34960229772011
41	31.94994666084901	25.662152247712093	17.981396418153825	24.406504673285074
42	32.391076593491775	25.276149904448243	18.39464855547422	23.938124946585763
43	31.738201016652134	23.96218012550742	19.220634046460624	25.07898481137982
44	29.954568204077475	24.296686364973038	20.21058247663494	25.538162954314547
45	28.237093959638088	24.349438471302935	20.668668443455175	26.7447991256038
46	28.910393229818705	22.534077942602323	21.76057151391719	26.794957313661786
47	28.2355649130778	21.73698050984419	22.827518360163097	27.199936216914917
48	29.463116256959548	21.23823828716204	22.349145222016194	26.94950023386221
49	29.446296744796395	21.671013072528957	21.35941522706478	27.523274955609867
50	28.923690474012627	23.126638093519215	20.741216241860215	27.20845519060794
51	28.602863740381	23.965292827433714	21.36943594291523	26.06240748927005
52	30.17993874530262	23.10968205934175	21.203070216275442	25.507308979080186
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	4.0
8	7.0
9	25.0
10	43.0
11	276.5
12	510.0
13	1019.5
14	2281.5
15	3034.0
16	3049.0
17	3064.0
18	3402.0
19	3740.0
20	4447.5
21	5155.0
22	6788.0
23	8421.0
24	12238.0
25	16055.0
26	20561.5
27	25068.0
28	28783.0
29	32498.0
30	32704.0
31	32910.0
32	31942.5
33	30975.0
34	31957.0
35	32939.0
36	32982.0
37	33025.0
38	35227.0
39	39515.5
40	41602.0
41	47789.5
42	53977.0
43	57147.0
44	60317.0
45	75923.0
46	91529.0
47	96310.5
48	101092.0
49	120131.5
50	139171.0
51	162096.5
52	185022.0
53	210542.5
54	236063.0
55	255315.0
56	274567.0
57	302136.0
58	329705.0
59	339957.0
60	350209.0
61	355606.5
62	361004.0
63	337926.0
64	287838.0
65	260828.0
66	222283.5
67	183739.0
68	164144.5
69	144550.0
70	127670.5
71	110791.0
72	91815.5
73	72840.0
74	57714.0
75	42588.0
76	33784.5
77	24981.0
78	17581.0
79	10181.0
80	7185.5
81	4190.0
82	3118.5
83	2047.0
84	1093.0
85	139.0
86	78.5
87	18.0
88	14.5
89	6.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3662413.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.677739024887902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.717574609707	8.191702403048177
2	8.141388081264857	1.73863234464158
3	3.631905939729499	1.163416313621155
4	2.1171424933150043	0.9042518008847237
5	1.3141027015561564	0.7015822849558395
6	0.9175925646906327	0.5878688362166486
7	0.6339324174035369	0.473827543871589
8	0.46272166684327604	0.39526569597709016
9	0.35870955680763794	0.34471863299926436
>10	2.65859623995509	5.642268756493352
>50	0.5277386478117649	4.154392545188
>100	2.2864029855030252	58.54611713974385
>500	0.21469817041567005	14.701858202904747
>1k	0.017493924996832375	2.4540974994539884
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0013925245459755632	0.0	0.0	0.0	0.0
12	0.0044233132636870825	0.0	0.0	0.0	0.0
13	0.01037567308766106	0.0	0.0	0.0	0.0
14	0.06236325613741541	0.0	0.0	0.0	0.0
15	0.12008476378824562	0.0	0.0	0.0	0.0
16	0.1520855239428213	0.0	0.0	0.0	0.0
17	0.17234539086662262	0.0	0.0	0.0	0.0
18	0.19347899868201648	0.0	0.0	0.0	0.0
19	0.21707000275501426	0.0	0.0	0.0	0.0
20	0.24431979681155566	0.0	0.0	0.0	0.0
21	0.2701770663221215	0.0	0.0	0.0	0.0
22	0.2988193849246385	0.0	0.0	0.0	0.0
23	0.3257961349525572	0.0	0.0	0.0	0.0
24	0.35487532400087046	0.0	0.0	0.0	0.0
25	0.3867395621411348	0.0	0.0	0.0	0.0
26	0.42791460165743184	0.0	0.0	0.0	0.0
27	0.4711101669855366	0.0	0.0	0.0	0.0
28	0.5163808669311735	0.0	0.0	0.0	0.0
29	0.570225149375562	0.0	0.0	0.0	0.0
30	0.6331617979730849	0.0	0.0	0.0	0.0
31	0.686760340791713	0.0	0.0	0.0	0.0
32	0.7289183388110516	0.0	0.0	0.0	0.0
33	0.7681274613212655	0.0	0.0	0.0	0.0
34	0.8059986680912283	0.0	0.0	0.0	0.0
35	0.8402929980862344	0.0	0.0	0.0	0.0
36	0.8749149809155876	0.0	0.0	0.0	0.0
37	0.9122947084340297	0.0	0.0	0.0	0.0
38	0.9496198271467472	0.0	0.0	0.0	0.0
39	0.9926788704605406	0.0	0.0	0.0	0.0
40	1.0366389590687888	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	70	0.0	46.000004	15
CGTATGT	35	1.0203985E-7	46.000004	31
CAATTTA	35	1.0203985E-7	46.000004	45
ATACGTT	35	1.0203985E-7	46.000004	33
TCGAATA	35	1.0203985E-7	46.000004	15
GCGTAAT	35	1.0203985E-7	46.000004	15
ACGCGTA	35	1.0203985E-7	46.000004	28
TTAGGTA	25	3.4186298E-5	46.0	27
TAATACT	40	5.6170393E-9	46.0	34
TAATACA	20	6.3132716E-4	46.0	32
CGAGTTA	65	0.0	46.0	25
CGTATAG	25	3.4186298E-5	46.0	45
TTTACTA	20	6.3132716E-4	46.0	26
AGATATA	45	3.110472E-10	46.0	30
CGACGAA	45	3.110472E-10	46.0	16
ACGTTAT	20	6.3132716E-4	46.0	45
ATAATAC	20	6.3132716E-4	46.0	31
CATGACG	25	3.4186298E-5	46.0	24
ACTCTAT	30	1.8625269E-6	46.0	24
ACCGAAT	40	5.6170393E-9	46.0	27
>>END_MODULE