FastQCFastQC Report
Fri 17 Jun 2016
SRR1528003_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528003_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3609885
Sequences flagged as poor quality0
Sequence length59
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAT457.6397555E-1153.036
ACGTTAT203.139147E-453.045
ACGTTAC307.014696E-753.035
CCAATAG307.014696E-753.049
TAAACGT150.006697422453.025
TAAGTAC401.6025297E-953.034
TAACGCG203.139147E-453.024
TCGATAA150.006697422453.035
GTTAATC150.006697422453.036
CACTAAT150.006697422453.045
CGCTAAG203.139147E-453.033
ACGGATA457.6397555E-1153.043
CGTACTA150.006697422453.039
CATACGT307.014696E-753.048
GCGATAA203.139147E-453.030
CGTACCA457.6397555E-1153.026
TTAACCG203.139147E-453.031
CGTACAT307.014696E-753.052
CATAACG150.006697422453.035
TTACGAT401.6025297E-953.040