##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528003_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3609885
Sequences flagged as poor quality	0
Sequence length	59
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.883423987190728	31.0	31.0	33.0	30.0	33.0
2	31.44120823793556	31.0	31.0	33.0	30.0	34.0
3	31.979557243513298	31.0	31.0	34.0	30.0	34.0
4	35.760000387823986	37.0	35.0	37.0	35.0	37.0
5	35.77922953224272	37.0	35.0	37.0	35.0	37.0
6	35.77828296469278	37.0	35.0	37.0	35.0	37.0
7	35.642701083275504	37.0	35.0	37.0	33.0	37.0
8	31.758903399969807	33.0	31.0	35.0	27.0	35.0
9	36.274172999970915	37.0	35.0	39.0	32.0	39.0
10	37.548504453742986	39.0	37.0	39.0	35.0	39.0
11	37.37979270807796	39.0	37.0	39.0	35.0	39.0
12	37.699129196636456	39.0	37.0	39.0	35.0	39.0
13	37.76381685289144	39.0	37.0	39.0	35.0	39.0
14	38.112214378020354	40.0	38.0	40.0	34.0	40.0
15	37.444725247480186	40.0	37.0	40.0	32.0	40.0
16	37.018377870763196	39.0	36.0	40.0	32.0	40.0
17	36.986354689969346	39.0	36.0	40.0	32.0	40.0
18	36.95777621724792	39.0	36.0	40.0	31.0	40.0
19	37.125061601685374	39.0	36.0	40.0	32.0	40.0
20	37.26778415378883	39.0	36.0	40.0	32.0	40.0
21	37.238472970745605	40.0	36.0	40.0	32.0	40.0
22	37.162005991880626	39.0	36.0	40.0	32.0	40.0
23	37.076677511887496	39.0	36.0	40.0	31.0	40.0
24	37.05176868515202	39.0	36.0	40.0	31.0	40.0
25	36.93093768915076	39.0	35.0	40.0	31.0	40.0
26	36.75032971964481	39.0	35.0	40.0	31.0	40.0
27	36.58563361436722	39.0	35.0	40.0	30.0	40.0
28	36.567071527209315	39.0	35.0	40.0	30.0	40.0
29	36.52581176408667	39.0	35.0	40.0	30.0	40.0
30	36.46293912409952	39.0	35.0	40.0	30.0	40.0
31	36.431889935551965	39.0	35.0	40.0	30.0	40.0
32	36.2627103079461	39.0	35.0	40.0	30.0	40.0
33	36.08113000829666	38.0	35.0	40.0	30.0	40.0
34	35.97141432483306	38.0	35.0	40.0	30.0	40.0
35	35.78213821215911	38.0	35.0	40.0	29.0	40.0
36	35.55385587075489	38.0	34.0	40.0	28.0	40.0
37	35.35921393617802	38.0	34.0	40.0	28.0	40.0
38	35.107877951790705	37.0	34.0	40.0	27.0	40.0
39	34.89828290928936	37.0	33.0	40.0	27.0	40.0
40	34.645876253675674	37.0	33.0	40.0	26.0	40.0
41	34.48203114503647	36.0	33.0	40.0	26.0	40.0
42	34.28126380757282	36.0	33.0	39.0	25.0	40.0
43	33.980295494177795	35.0	33.0	39.0	24.0	40.0
44	33.72820048284087	35.0	33.0	39.0	23.0	40.0
45	33.505972904954035	35.0	33.0	39.0	23.0	40.0
46	33.27852826336573	35.0	32.0	39.0	23.0	40.0
47	33.1304299167425	35.0	32.0	38.0	23.0	40.0
48	32.903116304259	35.0	32.0	38.0	23.0	40.0
49	32.76354953135626	35.0	32.0	38.0	22.0	40.0
50	32.50488395059676	35.0	31.0	37.0	22.0	40.0
51	32.26018058747024	35.0	31.0	37.0	20.0	40.0
52	32.029601774017735	35.0	31.0	37.0	20.0	39.0
53	31.74601517776882	35.0	31.0	36.0	20.0	39.0
54	31.530646544141987	34.0	30.0	36.0	20.0	39.0
55	31.33321587806814	34.0	30.0	35.0	19.0	39.0
56	31.094347880888172	34.0	30.0	35.0	18.0	38.0
57	30.88820391785334	34.0	30.0	35.0	18.0	38.0
58	30.662259601067625	34.0	30.0	35.0	17.0	38.0
59	30.122936880260728	34.0	29.0	35.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	2.0
13	10.0
14	35.0
15	81.0
16	349.0
17	900.0
18	2108.0
19	4239.0
20	7526.0
21	11711.0
22	17069.0
23	24026.0
24	32622.0
25	42369.0
26	53897.0
27	67516.0
28	80282.0
29	94117.0
30	111615.0
31	135511.0
32	165826.0
33	212104.0
34	293699.0
35	383711.0
36	534742.0
37	688108.0
38	584181.0
39	61525.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.13150141902027	19.304243763998024	34.453867643983116	19.110387172998585
2	16.110319303800537	24.398117945585522	35.2328952307345	24.25866751987944
3	17.41579025370614	25.609015245638016	37.805497959076256	19.16969654157958
4	19.68788479411394	24.505739102492186	32.85398288311123	22.95239322028264
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.43122841863384	0.17488091725913707	0.307738335154721	0.08615232895230734
12	99.17459974486722	0.4618983707237211	0.18341304501389932	0.1800888393951608
13	97.71638154678057	1.3006508517584356	0.398184429697899	0.5847831717630895
14	58.31387980503534	24.67103522688396	7.060280313638801	9.9548046544419
15	32.38862179820133	25.14373726586858	19.80096318857803	22.66667774735206
16	27.82418276482492	18.668572544554742	28.013690186806507	25.493554503813833
17	24.004698210607817	18.258504079769857	30.065472999832405	27.671324709789925
18	24.550228054356303	19.729714381483067	29.385562143946416	26.334495420214214
19	26.02672938334601	18.176146885565604	28.99363830149714	26.803485429591245
20	30.210103645961023	17.36304619122216	25.031074397106835	27.39577576570999
21	31.33036093947591	19.640487162333425	20.17596128408523	28.853190614105433
22	31.30515238020048	19.80373335992698	21.4546446770465	27.436469582826046
23	29.298135536173593	20.16504680897037	22.60462590913561	27.93219174572043
24	30.243013281586535	20.78343215919621	22.2441435114969	26.729411047720358
25	33.12061187544755	19.055676288856844	22.500051940712794	25.323659894982804
26	31.897581224886668	19.097810595074357	21.706397849238964	27.29821033080001
27	32.409508890172404	21.08391264541668	20.926262193948006	25.58031627046291
28	31.73893351173237	20.723928878620786	20.03307584590645	27.504061763740395
29	30.504129632938447	21.10740369845577	20.35701968345252	28.03144698515327
30	31.279251278087806	20.952468014909062	19.37912149556011	28.389159211443022
31	29.495621051640146	20.73135293783597	20.66545056144448	29.107575449079405
32	31.44828713380066	22.986604836442158	18.470532994818395	27.094575034938785
33	30.31739238230581	23.473019223604076	20.276684714333005	25.932903679757114
34	30.11591782009676	23.70540889806739	19.85406737333738	26.324605908498473
35	31.41590383073145	23.562966687304442	19.69184613914294	25.329283342821167
36	33.096151262436344	22.477419640791883	19.30809430217306	25.118334794598717
37	32.22905992850188	23.382822444482304	19.841379988559193	24.546737638456626
38	30.87710550336091	25.188807953715976	21.392759049110982	22.54132749381213
39	31.821761635065936	24.45557129936272	21.517693776948573	22.204973288622767
40	32.38488206688025	24.33925180442036	19.038335016212425	24.237531112486963
41	31.83985085397457	25.915811722534098	17.867716007573648	24.376621415917683
42	32.252440174687	25.551340278152907	18.306815868095523	23.889403679064568
43	31.798658406015708	24.13553340341867	19.087228540521373	24.978579650044253
44	29.696430772725446	24.477538758159888	20.23358084814336	25.5924496209713
45	28.04153040886344	24.536044777049685	20.595171314321647	26.82725349976523
46	28.709252510814053	22.741029146357846	21.772881961613734	26.77683638121436
47	28.1214775539941	21.94200092246706	22.670389776959652	27.266131746579187
48	29.32148808064523	21.42051616602745	22.265335322316364	26.99266043101096
49	29.380381923523878	21.85008663710894	21.265358868772825	27.504172570594353
50	28.66897421940034	23.373265353328428	20.656475206273882	27.301285220997347
51	28.48087958480672	24.10744386594033	21.369600416633773	26.042076132619187
52	29.972201330513297	23.236501993830828	21.260206350063786	25.53109032559209
53	28.457887162610447	23.350965473969392	21.603375176771557	26.587772186648607
54	26.981441237047715	23.25007583344068	22.50514905599486	27.26333387351675
55	28.06596332016117	24.001374004989078	20.859390257584383	27.073272417265372
56	29.000923852144872	23.601832191330193	20.94598581395252	26.45125814257241
57	29.298024729319632	22.92646441645648	20.03928102972809	27.736229824495794
58	29.078294737921013	22.313342391793643	20.32092435077572	28.28743851950962
59	28.025214099618132	22.875327053354884	21.462262648256107	27.637196198770873
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.0
7	4.0
8	10.0
9	206.5
10	397.0
11	698.0
12	999.0
13	1531.0
14	1989.5
15	1916.0
16	1969.0
17	2022.0
18	2159.5
19	2490.0
20	2683.0
21	3641.0
22	4599.0
23	6155.0
24	7711.0
25	10846.5
26	17287.0
27	20592.0
28	23085.0
29	25578.0
30	27388.5
31	31237.5
32	33276.0
33	33640.0
34	34004.0
35	35535.5
36	37594.5
37	38122.0
38	39288.5
39	40455.0
40	41178.5
41	41902.0
42	45137.5
43	55648.0
44	62923.0
45	66786.5
46	70650.0
47	79439.5
48	93615.0
49	99001.0
50	116796.0
51	134591.0
52	155267.5
53	186830.5
54	197717.0
55	220836.5
56	243956.0
57	264063.0
58	297659.0
59	311148.0
60	317459.0
61	323770.0
62	308258.0
63	292746.0
64	263560.5
65	213436.5
66	192498.0
67	171789.5
68	151081.0
69	135739.0
70	103514.5
71	86632.0
72	74771.5
73	62911.0
74	53279.5
75	34486.0
76	25324.0
77	18686.0
78	12048.0
79	9203.0
80	6358.0
81	3946.5
82	990.5
83	446.0
84	360.5
85	275.0
86	225.5
87	113.5
88	51.0
89	38.0
90	25.0
91	13.5
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	3609885.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.776768401744004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.61783874868286	12.208246690419182
2	6.719660727150728	1.9858974060680201
3	2.788549541763926	1.236172522663047
4	1.6061236082193244	0.9493326633292152
5	0.9532313147866881	0.7042839185946411
6	0.623041543536214	0.5523924356099843
7	0.4236001100370451	0.43816085046794834
8	0.29577706918022656	0.3496503399858258
9	0.2293600985411614	0.3050280951069532
>10	1.6130317756197876	4.827727553214764
>50	0.36731441960193295	4.027655181055391
>100	1.6056716358720187	56.28375717837499
>500	0.14481110283155527	13.811276149503943
>1k	0.011988304176593932	2.320419015606146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	8.033496911951488E-4	0.0	0.0	0.0	0.0
12	0.003656626180612402	0.0	0.0	0.0	0.0
13	0.008587531181741246	0.0	0.0	0.0	0.0
14	0.05689931950740813	0.0	0.0	0.0	0.0
15	0.11357702530690036	0.0	0.0	0.0	0.0
16	0.14288543817877855	0.0	0.0	0.0	0.0
17	0.15972807998038718	0.0	0.0	0.0	0.0
18	0.17997803254120284	0.0	0.0	0.0	0.0
19	0.2006435108043608	0.0	0.0	0.0	0.0
20	0.22455008954578887	0.0	0.0	0.0	0.0
21	0.24884449227606972	0.0	0.0	0.0	0.0
22	0.2740807532649932	0.0	0.0	0.0	0.0
23	0.2982643491413161	0.0	0.0	0.0	0.0
24	0.32560594035544066	0.0	0.0	0.0	0.0
25	0.35466503780591346	0.0	0.0	0.0	0.0
26	0.3930596127023437	0.0	0.0	0.0	0.0
27	0.4314264858852844	0.0	0.0	0.0	0.0
28	0.470181183057078	0.0	0.0	0.0	0.0
29	0.5177450251185287	0.0	0.0	0.0	0.0
30	0.575503097744111	0.0	0.0	0.0	2.7701713489487894E-5
31	0.6240642014911832	0.0	0.0	0.0	2.7701713489487894E-5
32	0.6620709523987606	0.0	0.0	0.0	2.7701713489487894E-5
33	0.6957008325749989	0.0	0.0	0.0	2.7701713489487894E-5
34	0.7280564339307208	0.0	0.0	0.0	2.7701713489487894E-5
35	0.759747194162695	0.0	0.0	0.0	2.7701713489487894E-5
36	0.7907731132709214	0.0	0.0	0.0	2.7701713489487894E-5
37	0.823461135188517	0.0	0.0	0.0	2.7701713489487894E-5
38	0.8589193284550616	0.0	0.0	0.0	2.7701713489487894E-5
39	0.8972862016380023	0.0	0.0	0.0	2.7701713489487894E-5
40	0.9365672313660961	0.0	0.0	0.0	2.7701713489487894E-5
41	0.9754050336783582	0.0	0.0	0.0	2.7701713489487894E-5
42	1.01695760391259	0.0	0.0	0.0	2.7701713489487894E-5
43	1.0554352839494887	0.0	0.0	0.0	2.7701713489487894E-5
44	1.0896746018224956	0.0	0.0	0.0	2.7701713489487894E-5
45	1.1270442133198149	0.0	0.0	0.0	2.7701713489487894E-5
46	1.1608126020635006	0.0	0.0	0.0	2.7701713489487894E-5
47	1.1928357828573486	0.0	0.0	0.0	2.7701713489487894E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAT	45	7.6397555E-11	53.0	36
ACGTTAT	20	3.139147E-4	53.0	45
ACGTTAC	30	7.014696E-7	53.0	35
CCAATAG	30	7.014696E-7	53.0	49
TAAACGT	15	0.0066974224	53.0	25
TAAGTAC	40	1.6025297E-9	53.0	34
TAACGCG	20	3.139147E-4	53.0	24
TCGATAA	15	0.0066974224	53.0	35
GTTAATC	15	0.0066974224	53.0	36
CACTAAT	15	0.0066974224	53.0	45
CGCTAAG	20	3.139147E-4	53.0	33
ACGGATA	45	7.6397555E-11	53.0	43
CGTACTA	15	0.0066974224	53.0	39
CATACGT	30	7.014696E-7	53.0	48
GCGATAA	20	3.139147E-4	53.0	30
CGTACCA	45	7.6397555E-11	53.0	26
TTAACCG	20	3.139147E-4	53.0	31
CGTACAT	30	7.014696E-7	53.0	52
CATAACG	15	0.0066974224	53.0	35
TTACGAT	40	1.6025297E-9	53.0	40
>>END_MODULE