FastQCFastQC Report
Fri 17 Jun 2016
SRR1528002_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528002_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496046
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTGAAGCGGGGACACAGGATCTGGAGCTGGTGCTGACAACAGCGGAATC24300.48987392298294913No Hit
GAATTGAAGCGGGGACACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAA20250.4082282691524577No Hit
GGATTGAAGCGGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTC18540.3737556597573612No Hit
TCACTGAAGCGGGATCGCTGAGAGCCCCTCCCCATGGCTCACTGAGGAGCAC14220.28666696233817024No Hit
TACTTGAAGCGGGGCTCGCTGGCATCTCTCTGGCTCTGCGCGCCCGGCGCTC13270.2675155126742278No Hit
ACCATGAAGCGGGCAGCATCTGCTGACACTGCCCTGGGGACAGTGACCCCAG12770.2574358023247844No Hit
CAACTGAAGCGGGGGGTTCACGACATTCTCCTGCCTCAGCCTCCCAAGTAGC12300.24796087459630758No Hit
TCGGTGAAGCAGGGGACGCCATTTGCCACTGCCGAGCGTGGGCACGGGAGGC12280.24755768618232987No Hit
CCTTTGAAGCGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCC11430.23042217858827607No Hit
TGGGTGAAGCGGGAGTGCCTTGAAGCGGGGCCTGACCGAGAGGATCCACCTG10980.221350439273777No Hit
ACTTTGAAGCGGGACTTGCAAATTCCAAAAACAGAGTGTTTCAACTCTGCTC10950.22074565665281043No Hit
ATGATGAAGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATT10440.21046435209637815No Hit
TTTGTGAAGCGGGGTGGTCGCGGAGGGGGAGGGGATGTCGGTCAGTGCGAGA10260.20683565637057855No Hit
GGGATGAAGCGGGGAACGTGGCCGAGGTTGATAGGACCACAGGCCGGTTTAA10140.2044165258867121No Hit
GCATTGAAGCGGGGGCTCAGAAACTGCCAGCCGAGATCACAAGCATTGGGGC9970.20098942436790135No Hit
GACTTGAAGCGGGGCTCTTGCTCGACGCGCGCGCAGCGCTAGGAGGGAGTTC9770.19695754022812398No Hit
TGGTTGAAGCGGGTGCAGCCCGGGCGCAGGCTTCCTTCTGAGCCTCTCACCC9630.19413522133027986No Hit
ATATTGAAGCGGGGATCTGCCTTTAACTACAGGGAGTAGGGTCTTCCTTCCA9620.19393362712329099No Hit
CTTATGAAGCGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCT9610.19373203291630212No Hit
TGGGTGAAGCGGGGGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATT9450.19050652560448023No Hit
TGTGTGAAGCGGGGATCACGCTCCTAGAACTCTTCAAACCGATCTCTCGTCG9350.18849058353459155No Hit
TTGCTGAAGCGGGGTTCCGGTGTCAGTTCGAGGCGCCGCCGCCGCCGCTGCA9330.18808739512061382No Hit
GTTCTGAAGCGGGGAGTGGTGTGAAGCGGGCACTGTTTGCTGCTTTAGGTGT9100.18345072835986986No Hit
GACGTGAAGCGGGGTCCTGCCTGAGGTGAGCTGCCATCATGGAGACCGAACC8970.18083000366901456No Hit
GATTTGAAGCGGGTGTGTGGGGCTGGGTGCACAGGAGGCCGCACTGTCCTAG8400.16933913387064908No Hit
ATGTTGAAGCGGGGACTTGCCTACTTGGGGCGCGAGGAGGTTGGAGAGTTTT8330.16792797442172702No Hit
CAGCTGAAGCGGGGAGTGGATTGAAGCGGGGGATCGTTCTGATACCAGACGC8300.1673231918007604No Hit
TGCTTGAAGCGGGGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGC8130.16389609028194965No Hit
AGGCTGAAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG7850.15825145248626135No Hit
GACTTGAAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG7850.15825145248626135No Hit
TTTTTGAAGCGGGGGAGACACTGCAGCTTGGCAAAGCCCTTCCTGGCTCGGA7760.15643710462336155No Hit
ACCTTGAAGCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGC7740.1560339162093838No Hit
TTGGTGAAGCGGGGACCACGACTTCAACGTCCTGAGTACCTTCTCCTCACTT7720.15563072779540607No Hit
ATATTGAAGCGGGGTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCA7720.15563072779540607No Hit
CGTTTGAAGCGGGGGCGGCGGGCTGGAGGGTCAGGGGCTGCGCACGCTGGCG7510.15139724944863983No Hit
CACTTGAAGCGGGAGTGCCTCTGCTGCTGGACTGTAAATGTTTTCTGAGTTT7480.15079246682767325No Hit
AGGTTGAAGCGGGGAGACCTTCTGGCCGGAAACCCGCGCGCTCCCGCTACCA7440.14998608999971777No Hit
GGTTTGAAGCGGGGGAGGAAAAGGAGCTTGGTGGAGAGAGAGAGAGAGAGAG7410.14938130737875116No Hit
AGACTGAAGCGGGGGTAAAGGAGTAGGAGAGCGGCTATGGCGAGTCCTGCTG7280.14676058268789588No Hit
ACTTTGAAGCGGTCGCCCCCCGGGCCGCGGTTTTCCGCGCGGCGCCTCGCCT7250.14615580006692927No Hit
CGCATGAAGCGGGGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGA7230.14575261165295153No Hit
CTTTTGAAGCGGGCTTTTTCCTCAGCTGCCGTGCCGGCAGTCTACAGCATGG7210.14534942323897382No Hit
GGGCTGAAGCGGGGCCTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCT7190.14494623482499608No Hit
GGGATGAAGCGGGCTAGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCG7150.1441398579970406No Hit
GGCCTGAAGCGGGGGTGCTGCTATCACATCCTCAACAGAGAGGCAAGGTAAC7120.143535075376074No Hit
GTCTTGAAGCGGGGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACGGCG7110.14333348116908512No Hit
CTATTGAAGCGGGGGGAACTTTCCAGAACGCTCGGTGGGTGGTGGAGGAGCA7050.1421239159271519No Hit
AGACTGAAGCGGGGCTGTGAGCGAGTACAACAAGGGCAGCAACGATGCGTAC7010.14131753909919645No Hit
AGTTTGAAGCGGGCGTATGAAGCGGGACCTCAACTGGTCTTTCAGACATGAC6890.13889840861533No Hit
ACATTGAAGCGGGGGAAGTGGGCAACCAGGCCCGTGACCGCCTAGAGGAGGT6850.13809203178737456No Hit
CGTCTGAAGCGGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGC6840.1378904375803857No Hit
ACTGTGAAGCGGGGAGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTA6830.13768884337339682No Hit
ACGATGAAGCGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCC6800.13708406075243024No Hit
GCACTGAAGCGGGGGGCCATCTTGACTCCCTGACCTTGGACTGGTCCTCGTC6800.13708406075243024No Hit
CTTCTGAAGCGGGGCATTGCGGGAGTAGCAGCAGCGGCCAGGCAGCATCCAG6780.13668087233845247No Hit
AGTATGAAGCGGGCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAG6690.13486652447555267No Hit
TGGGTGAAGCGGGGCCGTTCCGCGCATGAGCAGTGAGGCTTGGAGCCCGCCT6660.13426174185458606No Hit
CGAATGAAGCGGGGGCCGGGCAGTGGTGGCACACGCCTTTCATCCCAGCACT6650.13406014764759722No Hit
GATGTGAAGCGGGATAAAAATGGTCTATGTAACCTATGAAAGTATTGCATTT6570.13244739399168626No Hit
GGGATGAAGCGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATG6520.1314394229567419No Hit
GTATTGAAGCGGGGACGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACG6480.13063304612878646No Hit
AGCATGAAGCGGGAGGTGGTTGGCAATCAAGATGAAATACTAGAAGAGGCGC6460.13022985771480872No Hit
TGTTTGAAGCGGGCGGCCAGCGCTCAGGGTCCTTGGTGAAAAGATGCCTAGA6440.12982666930083098No Hit
CCGATGAAGCGGGGGACCCCACACCATTCTGCCTTCCAGGAAAACTGCCTGA6440.12982666930083098No Hit
TATTTGAAGCGGGGTGCCTAGAGGAGGTGCGTGAGCACATGGAGGAGGTGCG6370.1284155098519089No Hit
TTTGTGAAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCT6340.1278107272309423No Hit
GGTTTGAAGCGGGTGATGCCGGTGCCATCCTTCCTGTAGACCGCGACTCGGA6330.12760913302395344No Hit
GGCTTGAAGCGGGGGCAGCTGAGTGTGACAGACAGAGCCGGCCGGCCGCCTG6330.12760913302395344No Hit
AGTTTGAAGCGGGGTGGAGAGAGGGGAAGAAAGAAAGCACTGAGTCATCCAT6230.12559319095406474No Hit
CCGGTGAAGCGGGGAGTTCCACGACTCAAGGAACACTCAGTGCTTCGCACCC6200.12498840833309814No Hit
TCTTTGAAGCGGGCTTTCCAGCCTCCGCCTCGGACCCTGCAGCCGCCGCGAT6180.12458521991912042No Hit
CGCTTGAAGCGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCAT6160.12418203150514266No Hit
GGTTTGAAGCGGGGATTGCGGGAGTAGCAGCAGCGGCCAGGCAGCATCCAGC6140.12377884309116494No Hit
TGGTTGAAGCGGGACCTCCGAAGCCTCCTTCTGTTGCCATTTTGCTGAGCAT6120.12337565467718721No Hit
CTCTTGAAGCGGGAAAGAAGGGCAGACGGTGGATGCCTTGGCTCTCGGAGGC6030.12156130681428738No Hit
TGTCTGAAGCGGGATGGGCCGCCGCCCCGCCCGGTGTTACCGGTATTGTAAG6030.12156130681428738No Hit
ATACTGAAGCGGGGCCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACGCC6010.12115811840030964No Hit
TTTATGAAGCAGGTCTCCCTCTGGGTGCTCCATCCCCCACCCAGAGGCGAGT5990.12075492998633192No Hit
ACATTGAAGCGGGAACCTTGGAGCTGGAAAAGAATATGGTAATGCGTAAGCA5920.11934377053740984No Hit
TCCTTGAAGCGGGGAGCGAGGCTGCTGTGGTCTACACGACTCTCTGAGCTTC5860.11813420529547664No Hit
TTTCTGAAGCGGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGAC5840.1177310168814989No Hit
CGTATGAAGCGGGGAATACAGGCTGCCCTTGGACTCCTGATCTCCCTCCCCA5830.11752942267451003No Hit
GGCATGAAGCGGGGAGACTTTGGCCTTAGCTGTAGCTAGTGTGGGAGCCTGG5820.11732782846752116No Hit
AGTTTGAAGCGGGGCTGGTGAAGGGGCTGGAGGCGCCATGTCGGGCCGGGAA5810.1171262342605323No Hit
TCTGTGAAGCGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTT5800.11692464005354342No Hit
AACCTGAAGCGGGAGTCCACTAAGGGAAGCCTAGGATGGAACTCAAACCAGC5780.11652145163956569No Hit
CTTATGAAGCGGGAGAACTGCCTCCACGTACGTCAGTCACCTGGAGACGCAC5750.11591666901859907No Hit
TTTTTGAAGCGGGGGGCCCTGGAGCCAGGAGCTGATGCAGAGGCCATGGAGG5690.11470710377666586No Hit
TGTCTGAAGCGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCG5690.11470710377666586No Hit
GCACTGAAGCGGGGAGTAATCTGAAGCGGGGCCTTTTCCGGTCGCGGCGCTG5650.11390072694871041No Hit
GTCGTGAAGCGGGGAGTGCTGCCTTTGCTCCGCACCATGAACCACACTTCTC5640.11369913274172153No Hit
TGTTTGAAGCGGGGAGCTTCTTCGGAGCGCAAGGGCGAGTGCCAGCCCAGCT5630.11349753853473266No Hit
TGTTTGAAGCGGAAAGAAAACCAACTACCTCGTCATGGGCCGCGACAGTGGG5540.11168319067183284No Hit
TTTTTGAAGCGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAG5530.11148159646484398No Hit
CGTGTGAAGCGGGGGAGGAGGAGGAGGAGGACTCCGATGGGTCGAAGTCGTA5510.11107840805086626No Hit
GTTCTGAAGCGGGGAGTGCCAGCGCTCTCCCCGGATAGAGCGGGGCCCGAGC5480.11047362542989964No Hit
AGGATGAAGCGGGGCCCCGCCGTCCCGCGACCTGCCGCCCCCTGCCCGGACT5470.11027203122291078No Hit
GCTCTGAAGCGGGACTACGAGCCCATGCACGACGTCTGCTGCTCCCGCTGTC5460.11007043701592191No Hit
CGTCTGAAGCGGGGAAAGTGGCGCTTTCTCCGCCCCAGGGTTCCAGGACATA5430.1094656543949553No Hit
CAGCTGAAGCGGGGTTGACAGAAAGAAGGAAATAATGAAGGTCAGAACAGAA5420.10926406018796643No Hit
GTCTTGAAGCGGGGGGTGCTTGACGCTGCTGGCGTGGATAAGTGGACATGGC5370.1082560891530221No Hit
TGTCTGAAGCGGGCCCCGCCGTCCCGCGACCTGCCGCCCCCTGCCCGGACTG5360.10805449494603322No Hit
GATCTGAAGCGGGGAGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGT5330.10744971232506662No Hit
TCTTTGAAGCGGGGGAGAAGGCTGTAATGGCGGCTGTGACTGAAGTTGTGGT5330.10744971232506662No Hit
TGCATGAAGCGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATG5310.10704652391108889No Hit
TATTTGAAGCGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGG5310.10704652391108889No Hit
TTTCTGAAGCGGGTATTTGTGGTTCACAAATTTGAAAGCCACTGGACCAGAT5300.10684492970410003No Hit
GGTATGAAGCGGGGGTGCTGCCGGAAGAGAGGTAAAGCCGGGTCACCTCTGA5280.10644174129012228No Hit
TAGGTGAAGCGGGACGTGGAGGCGCCGGTGAGTAAAATGCTGCAGATTCTGG5260.10603855287614455No Hit
CTTGTGAAGCGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCG5250.10583695866915568No Hit
TGGTTGAAGCGGGCATGTGGTGGCCTTGCCTGTGCATCTCCCAGCTGCTTTG5240.10563536446216683No Hit
GTGCTGAAGCGGGAGAACCCGGATGCAGCCCTGCGAACCCTGCACCAGGGCG5200.10482898763421135No Hit
CTTCTGAAGCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC5170.10422420501324473No Hit
GGTTTGAAGCGGGATTCCATGTATTTTCCTTCAGATGGCAGGATTCCCTTTT5160.10402261080625587No Hit
TAAATGAAGCGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGC5160.10402261080625587No Hit
AAAGTGAAGCGGGGCGCGTGCGCGAGGTCAGGAGCAGGAATCCTGTTCCCTA5150.103821016599267No Hit
CAAATGAAGCGGGGACAGAGGTGGCTATGGAGGAGACAGAGGTGGCTATGGA5130.10341782818528927No Hit
TTTTTGAAGCGGGGAGACCTGCAAACTTGTGCCACCGGCTATCCTGCGTCCC5110.10301463977131153No Hit
CGGCTGAAGCGGGGGCCCTCGTCCTACAGCACAGGCCTCCCGGCTCCGGCTC5110.10301463977131153No Hit
TACTTGAAGCGGGGCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCA5100.10281304556432266No Hit
GACTTGAAGCGGGGTCCCACTATGAGCCTGGAGTCTGAGCAGCCAGATGACA5070.10220826294335605No Hit
ACTTTGAAGCGGGATTGCCAATTGAGCTGCTGATTCTTATGAATCACATTCA5060.10200666873636718No Hit
GGATTGAAGCGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCT5060.10200666873636718No Hit
TGCGTGAAGCGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACG5000.10079710349443398No Hit
TTATTGAAGCGGGAGCGCGTGGTGGGGGAACTGCTTGGGCAAAGCGGTTGGC4980.10039391508045625No Hit
ATATTGAAGCGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAG4980.10039391508045625No Hit
CCTTTGAAGCGGGAACTCTAGGAACAGGGGTGTAGAGGAGAAGCCCTAGGCT4970.10019232087346737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCAC206.3079665E-446.00000421
TCACGGT405.6006684E-946.00000432
CCACAAG206.3079665E-446.00000433
TGATCAG405.6006684E-946.00000441
ACAACTT206.3079665E-446.00000436
TATCATG405.6006684E-946.00000440
CTTAAGA206.3079665E-446.00000418
GCCCCCA206.3079665E-446.00000434
CTGCTAA206.3079665E-446.00000422
GTTTGAT206.3079665E-446.00000420
AACAATG206.3079665E-446.00000429
AGGTATG206.3079665E-446.00000415
AACCGTG206.3079665E-446.00000425
AACCGTC800.046.00000423
ATGGGCG206.3079665E-446.00000424
CATGGAT405.6006684E-946.00000429
TTAGGAT206.3079665E-446.00000430
CTGTCGG206.3079665E-446.00000419
CCTTACT405.6006684E-946.00000422
GTCGCGA206.3079665E-446.00000424
Produced by FastQC (version 0.11.5)