FastQCFastQC Report
Fri 17 Jun 2016
SRR1528001_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528001_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492612
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTGAAGCGGGGACACAGGATCTGGAGCTGGTGCTGACAACAGCGGAATC23400.47501887895544564No Hit
GAATTGAAGCGGGGACACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAA19040.3865110878338327No Hit
GGATTGAAGCGGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTC17220.34956517502618695No Hit
TCACTGAAGCGGGATCGCTGAGAGCCCCTCCCCATGGCTCACTGAGGAGCAC13000.2638993771974698No Hit
TACTTGAAGCGGGGCTCGCTGGCATCTCTCTGGCTCTGCGCGCCCGGCGCTC12850.2608543843836528No Hit
TCGGTGAAGCAGGGGACGCCATTTGCCACTGCCGAGCGTGGGCACGGGAGGC12520.25415540019325555No Hit
ACCATGAAGCGGGCAGCATCTGCTGACACTGCCCTGGGGACAGTGACCCCAG11440.23223145193377345No Hit
CAACTGAAGCGGGGGGTTCACGACATTCTCCTGCCTCAGCCTCCCAAGTAGC11400.2314194538500889No Hit
CCTTTGAAGCGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCC11210.22756246295258745No Hit
ACTTTGAAGCGGGACTTGCAAATTCCAAAAACAGAGTGTTTCAACTCTGCTC10900.2212694778040324No Hit
TGGGTGAAGCGGGAGTGCCTTGAAGCGGGGCCTGACCGAGAGGATCCACCTG10560.21436749409271394No Hit
ATGATGAAGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATT10470.21254049840442377No Hit
GACTTGAAGCGGGGCTCTTGCTCGACGCGCGCGCAGCGCTAGGAGGGAGTTC10010.20320252044205175No Hit
GCATTGAAGCGGGGGCTCAGAAACTGCCAGCCGAGATCACAAGCATTGGGGC9660.1960975372098122No Hit
TGTGTGAAGCGGGGATCACGCTCCTAGAACTCTTCAAACCGATCTCTCGTCG9590.19467654056336425No Hit
TTGCTGAAGCGGGGTTCCGGTGTCAGTTCGAGGCGCCGCCGCCGCCGCTGCA9410.1910225491867839No Hit
CTTATGAAGCGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCT9340.189601552540336No Hit
TTTGTGAAGCGGGGTGGTCGCGGAGGGGGAGGGGATGTCGGTCAGTGCGAGA9150.18574456164283454No Hit
GGGATGAAGCGGGGAACGTGGCCGAGGTTGATAGGACCACAGGCCGGTTTAA8980.18229356978717529No Hit
GACGTGAAGCGGGGTCCTGCCTGAGGTGAGCTGCCATCATGGAGACCGAACC8810.1788425779315161No Hit
TGGTTGAAGCGGGTGCAGCCCGGGCGCAGGCTTCCTTCTGAGCCTCTCACCC8740.1774215812850682No Hit
GTTCTGAAGCGGGGAGTGGTGTGAAGCGGGCACTGTTTGCTGCTTTAGGTGT8740.1774215812850682No Hit
TGGGTGAAGCGGGGGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATT8650.175594585596778No Hit
ATATTGAAGCGGGGATCTGCCTTTAACTACAGGGAGTAGGGTCTTCCTTCCA8640.17539158607585686No Hit
TGCTTGAAGCGGGGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGC7850.15935462392308755No Hit
GATTTGAAGCGGGTGTGTGGGGCTGGGTGCACAGGAGGCCGCACTGTCCTAG7620.15468563494190152No Hit
TTTTTGAAGCGGGGGAGACACTGCAGCTTGGCAAAGCCCTTCCTGGCTCGGA7610.1544826354209804No Hit
ACCTTGAAGCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGC7600.15427963590005928No Hit
CAGCTGAAGCGGGGAGTGGATTGAAGCGGGGGATCGTTCTGATACCAGACGC7590.15407663637913815No Hit
ATGTTGAAGCGGGGACTTGCCTACTTGGGGCGCGAGGAGGTTGGAGAGTTTT7580.153873636858217No Hit
ATATTGAAGCGGGGTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCA7540.1530616387745325No Hit
CACTTGAAGCGGGAGTGCCTCTGCTGCTGGACTGTAAATGTTTTCTGAGTTT7400.15021964548163666No Hit
CTTTTGAAGCGGGCTTTTTCCTCAGCTGCCGTGCCGGCAGTCTACAGCATGG7190.14595665554229292No Hit
CGTTTGAAGCGGGGGCGGCGGGCTGGAGGGTCAGGGGCTGCGCACGCTGGCG7160.14534765697952953No Hit
GGGATGAAGCGGGCTAGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCG7120.144535658895845No Hit
TATTTGAAGCGGGGTGCCTAGAGGAGGTGCGTGAGCACATGGAGGAGGTGCG7070.14352066129123933No Hit
AGGCTGAAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG7060.1433176617703182No Hit
GACTTGAAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG7020.1425056636866337No Hit
TTGGTGAAGCGGGGACCACGACTTCAACGTCCTGAGTACCTTCTCCTCACTT7000.14209966464479143No Hit
CTATTGAAGCGGGGGGAACTTTCCAGAACGCTCGGTGGGTGGTGGAGGAGCA6940.14088166751926465No Hit
ACATTGAAGCGGGGGAAGTGGGCAACCAGGCCCGTGACCGCCTAGAGGAGGT6910.14027266895650126No Hit
ACTTTGAAGCGGTCGCCCCCCGGGCCGCGGTTTTCCGCGCGGCGCCTCGCCT6880.1396636703937379No Hit
CTTCTGAAGCGGGGCATTGCGGGAGTAGCAGCAGCGGCCAGGCAGCATCCAG6850.13905467183097447No Hit
GCACTGAAGCGGGGGGCCATCTTGACTCCCTGACCTTGGACTGGTCCTCGTC6710.13621267853807864No Hit
AGGTTGAAGCGGGGAGACCTTCTGGCCGGAAACCCGCGCGCTCCCGCTACCA6700.13600967901715752No Hit
GGGCTGAAGCGGGGCCTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCT6690.1358066794962364No Hit
ACTGTGAAGCGGGGAGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTA6650.13499468141255186No Hit
GGTTTGAAGCGGGGGAGGAAAAGGAGCTTGGTGGAGAGAGAGAGAGAGAGAG6640.13479168189163074No Hit
AGACTGAAGCGGGGGTAAAGGAGTAGGAGAGCGGCTATGGCGAGTCCTGCTG6630.13458868237070962No Hit
AGTTTGAAGCGGGGTGGAGAGAGGGGAAGAAAGAAAGCACTGAGTCATCCAT6580.13357368476610396No Hit
GGCCTGAAGCGGGGGTGCTGCTATCACATCCTCAACAGAGAGGCAAGGTAAC6500.1319496885987349No Hit
GTCTTGAAGCGGGGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACGGCG6460.13113769051505036No Hit
AGCATGAAGCGGGAGGTGGTTGGCAATCAAGATGAAATACTAGAAGAGGCGC6440.13073169147320812No Hit
TTTGTGAAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCT6430.13052869195228697No Hit
AGACTGAAGCGGGGCTGTGAGCGAGTACAACAAGGGCAGCAACGATGCGTAC6410.13012269291044473No Hit
ACGATGAAGCGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCC6390.12971669386860246No Hit
TGTTTGAAGCGGGCGGCCAGCGCTCAGGGTCCTTGGTGAAAAGATGCCTAGA6390.12971669386860246No Hit
GGTTTGAAGCGGGTGATGCCGGTGCCATCCTTCCTGTAGACCGCGACTCGGA6370.12931069482676022No Hit
CGAATGAAGCGGGGGCCGGGCAGTGGTGGCACACGCCTTTCATCCCAGCACT6310.12809269770123344No Hit
CGCATGAAGCGGGGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGA6210.12606270249202212No Hit
AGTTTGAAGCGGGCGTATGAAGCGGGACCTCAACTGGTCTTTCAGACATGAC6210.12606270249202212No Hit
GGTTTGAAGCGGGGATTGCGGGAGTAGCAGCAGCGGCCAGGCAGCATCCAGC6190.12565670345017987No Hit
GATGTGAAGCGGGATAAAAATGGTCTATGTAACCTATGAAAGTATTGCATTT6160.12504770488741648No Hit
GTATTGAAGCGGGGACGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACG6140.12464170584557421No Hit
CGTCTGAAGCGGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGC6130.12443870632465306No Hit
CGTATGAAGCGGGGAATACAGGCTGCCCTTGGACTCCTGATCTCCCTCCCCA6090.12362670824096855No Hit
CCGGTGAAGCGGGGAGTTCCACGACTCAAGGAACACTCAGTGCTTCGCACCC6030.12240871111544177No Hit
TGGTTGAAGCGGGACCTCCGAAGCCTCCTTCTGTTGCCATTTTGCTGAGCAT6030.12240871111544177No Hit
GGGATGAAGCGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATG5970.12119071398991499No Hit
AACCTGAAGCGGGAGTCCACTAAGGGAAGCCTAGGATGGAACTCAAACCAGC5950.12078471494807272No Hit
GGCATGAAGCGGGGAGACTTTGGCCTTAGCTGTAGCTAGTGTGGGAGCCTGG5940.1205817154271516No Hit
AGTATGAAGCGGGCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAG5930.12037871590623045No Hit
ATACTGAAGCGGGGCCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACGCC5850.11875471973886141No Hit
TTTATGAAGCAGGTCTCCCTCTGGGTGCTCCATCCCCCACCCAGAGGCGAGT5840.11855172021794029No Hit
TGGGTGAAGCGGGGCCGTTCCGCGCATGAGCAGTGAGGCTTGGAGCCCGCCT5800.11773972213425578No Hit
CCGATGAAGCGGGGGACCCCACACCATTCTGCCTTCCAGGAAAACTGCCTGA5790.11753672261333463No Hit
TTTCTGAAGCGGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGAC5780.11733372309241351No Hit
TCTGTGAAGCGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTT5720.11611572596688673No Hit
TGTCTGAAGCGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCG5720.11611572596688673No Hit
TCTTTGAAGCGGGCTTTCCAGCCTCCGCCTCGGACCCTGCAGCCGCCGCGAT5700.11570972692504446No Hit
TGTTTGAAGCGGAAAGAAAACCAACTACCTCGTCATGGGCCGCGACAGTGGG5650.1146947293204388No Hit
AGTTTGAAGCGGGGCTGGTGAAGGGGCTGGAGGCGCCATGTCGGGCCGGGAA5640.11449172979951767No Hit
CTCTTGAAGCGGGAAAGAAGGGCAGACGGTGGATGCCTTGGCTCTCGGAGGC5630.11428873027859654No Hit
CTTGTGAAGCGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCG5590.11347673219491203No Hit
TCCTTGAAGCGGGGAGCGAGGCTGCTGTGGTCTACACGACTCTCTGAGCTTC5550.1126647341112275No Hit
CGTGTGAAGCGGGGGAGGAGGAGGAGGAGGACTCCGATGGGTCGAAGTCGTA5530.11225873506938525No Hit
TGTCTGAAGCGGGATGGGCCGCCGCCCCGCCCGGTGTTACCGGTATTGTAAG5530.11225873506938525No Hit
GGATTGAAGCGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCT5520.1120557355484641No Hit
CGTCTGAAGCGGGGAAAGTGGCGCTTTCTCCGCCCCAGGGTTCCAGGACATA5510.11185273602754298No Hit
CTTATGAAGCGGGAGAACTGCCTCCACGTACGTCAGTCACCTGGAGACGCAC5440.11043173938109507No Hit
CAGCTGAAGCGGGGTTGACAGAAAGAAGGAAATAATGAAGGTCAGAACAGAA5390.10941674177648941No Hit
ACATTGAAGCGGGAACCTTGGAGCTGGAAAAGAATATGGTAATGCGTAAGCA5380.10921374225556826No Hit
TGCATGAAGCGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATG5360.10880774321372602No Hit
TAAATGAAGCGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGC5360.10880774321372602No Hit
GGCTTGAAGCGGGGGCAGCTGAGTGTGACAGACAGAGCCGGCCGGCCGCCTG5350.10860474369280487No Hit
AGGATGAAGCGGGGCCCCGCCGTCCCGCGACCTGCCGCCCCCTGCCCGGACT5340.10840174417188375No Hit
GCACTGAAGCGGGGAGTAATCTGAAGCGGGGCCTTTTCCGGTCGCGGCGCTG5290.1073867465672781No Hit
TGTTTGAAGCGGGGAGCTTCTTCGGAGCGCAAGGGCGAGTGCCAGCCCAGCT5230.10616874944175132No Hit
TATTTGAAGCGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGG5210.10576275039990905No Hit
CGTTTGAAGCGGAGGGTGCGCTCTGTGACTTGATTGCTGCTGCTTCTGAGCT5190.10535675135806681No Hit
GTCTTGAAGCGGGGGGTGCTTGACGCTGCTGGCGTGGATAAGTGGACATGGC5140.10434175375346115No Hit
GGTTTGAAGCGGGATTCCATGTATTTTCCTTCAGATGGCAGGATTCCCTTTT5120.10393575471161888No Hit
AAAGTGAAGCGGGGCGCGTGCGCGAGGTCAGGAGCAGGAATCCTGTTCCCTA5100.10352975566977661No Hit
GCTCTGAAGCGGGACTACGAGCCCATGCACGACGTCTGCTGCTCCCGCTGTC5060.1027177575860921No Hit
GGTATGAAGCGGGGGTGCTGCCGGAAGAGAGGTAAAGCCGGGTCACCTCTGA5040.10231175854424983No Hit
GTTCTGAAGCGGGGAGTGCCAGCGCTCTCCCCGGATAGAGCGGGGCCCGAGC5040.10231175854424983No Hit
TCTTTGAAGCGGGGGAGAAGGCTGTAATGGCGGCTGTGACTGAAGTTGTGGT5000.10149976046056532No Hit
TTTTTGAAGCGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAG4990.10129676093964418No Hit
TTTTTGAAGCGGGGAGACCTGCAAACTTGTGCCACCGGCTATCCTGCGTCCC4980.10109376141872306No Hit
TGCGTGAAGCGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACG4980.10109376141872306No Hit
GTGCTGAAGCGGGAGAACCCGGATGCAGCCCTGCGAACCCTGCACCAGGGCG4980.10109376141872306No Hit
CGCTTGAAGCGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCAT4970.10089076189780193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCAA750.046.00000425
GGTAACG750.046.00000439
CTATCGA750.046.00000443
TAGACCG750.046.00000437
ATGGACG750.046.00000424
AACCAAC1500.046.00000419
CCAATAG750.046.00000444
ACCGAAC750.046.00000445
CGATTGA1500.046.0000041
GTTAATG750.046.00000432
CGTAGCG1500.046.00000416
TACGAGG1650.046.00000433
TCTCGAC750.046.00000424
ACGGACT750.046.00000428
TGTTAAT750.046.00000431
GGACGTA1500.046.00000413
GCCTACT750.046.00000419
CGATGAA3150.046.0000042
ACGTAGC1500.046.00000415
ATTATGA750.046.0000041
Produced by FastQC (version 0.11.5)