FastQCFastQC Report
Fri 17 Jun 2016
SRR1528000_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528000_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489303
Sequences flagged as poor quality0
Sequence length59
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTGAAGCGGGGACACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTG21140.43204313073903083No Hit
GGCTTGAAGCGGGGACACAGGATCTGGAGCTGGTGCTGACAACAGCGGAATCCCCTGTC19560.39975230072163875No Hit
GGATTGAAGCGGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGC16740.34211930031085036No Hit
TCACTGAAGCGGGATCGCTGAGAGCCCCTCCCCATGGCTCACTGAGGAGCACTCAGCCA13640.27876387432735955No Hit
CAACTGAAGCGGGGGGTTCACGACATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACT12400.2534217039339632No Hit
TCGGTGAAGCAGGGGACGCCATTTGCCACTGCCGAGCGTGGGCACGGGAGGCTCTCGCA12190.2491298847544364No Hit
ACCATGAAGCGGGCAGCATCTGCTGACACTGCCCTGGGGACAGTGACCCCAGCGGTCCC12190.2491298847544364No Hit
ACTTTGAAGCGGGACTTGCAAATTCCAAAAACAGAGTGTTTCAACTCTGCTCTCTCTAA11880.2427943421560873No Hit
TACTTGAAGCGGGGCTCGCTGGCATCTCTCTGGCTCTGCGCGCCCGGCGCTCGCTCGCT11790.24095499107914725No Hit
CCTTTGAAGCGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCT11350.23196260803632923No Hit
ATGATGAAGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT10620.21704342707892657No Hit
GCATTGAAGCGGGGGCTCAGAAACTGCCAGCCGAGATCACAAGCATTGGGGCTGAAGCA10550.21561282068575097No Hit
TGGGTGAAGCGGGAGTGCCTTGAAGCGGGGCCTGACCGAGAGGATCCACCTGCGAAACC10240.20927727808740187No Hit
CTTATGAAGCGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGCA10100.2064160653010507No Hit
GACTTGAAGCGGGGCTCTTGCTCGACGCGCGCGCAGCGCTAGGAGGGAGTTCCGTTTCC9810.20048926738646605No Hit
GGGATGAAGCGGGGAACGTGGCCGAGGTTGATAGGACCACAGGCCGGTTTAATGGCCAG9570.1955843311812926No Hit
GACGTGAAGCGGGGTCCTGCCTGAGGTGAGCTGCCATCATGGAGACCGAACCTCCTCCT9570.1955843311812926No Hit
TTGCTGAAGCGGGGTTCCGGTGTCAGTTCGAGGCGCCGCCGCCGCCGCTGCAGCCGCCG9440.1929274907368236No Hit
TTTGTGAAGCGGGGTGGTCGCGGAGGGGGAGGGGATGTCGGTCAGTGCGAGATCCGCTG9220.18843129921541457No Hit
TGTGTGAAGCGGGGATCACGCTCCTAGAACTCTTCAAACCGATCTCTCGTCGATCTTCA9090.18577445877094562No Hit
ATATTGAAGCGGGGATCTGCCTTTAACTACAGGGAGTAGGGTCTTCCTTCCAACTCAAT8770.1792345438307143No Hit
TGGGTGAAGCGGGGGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCA8630.17637333104436312No Hit
GTTCTGAAGCGGGGAGTGGTGTGAAGCGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTT8550.17473835230930526No Hit
TGGTTGAAGCGGGTGCAGCCCGGGCGCAGGCTTCCTTCTGAGCCTCTCACCCTCTGGGA8040.1643153628733116No Hit
GATTTGAAGCGGGTGTGTGGGGCTGGGTGCACAGGAGGCCGCACTGTCCTAGGTCCTCC7890.1612497777450782No Hit
CAGCTGAAGCGGGGAGTGGATTGAAGCGGGGGATCGTTCTGATACCAGACGCCCAGCGC7860.1606366607194315No Hit
TGCTTGAAGCGGGGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGCATCCGTA7650.15634484153990472No Hit
TTTTTGAAGCGGGGGAGACACTGCAGCTTGGCAAAGCCCTTCCTGGCTCGGAGGAAGAC7640.1561404691980225No Hit
ACCTTGAAGCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCG7420.15164427767661348No Hit
GACTTGAAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAG7340.15000929894155565No Hit
GGGATGAAGCGGGCTAGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTA7340.15000929894155565No Hit
CACTTGAAGCGGGAGTGCCTCTGCTGCTGGACTGTAAATGTTTTCTGAGTTTCATGTAC7300.14919180957402672No Hit
ATGTTGAAGCGGGGACTTGCCTACTTGGGGCGCGAGGAGGTTGGAGAGTTTTTTTCTGG7260.1483743202064978No Hit
CTATTGAAGCGGGGGGAACTTTCCAGAACGCTCGGTGGGTGGTGGAGGAGCAGCGTGTG7250.1481699478646156No Hit
AGGCTGAAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAG7240.14796557552273337No Hit
GATGTGAAGCGGGATAAAAATGGTCTATGTAACCTATGAAAGTATTGCATTTAAATTGC7240.14796557552273337No Hit
TTGGTGAAGCGGGGACCACGACTTCAACGTCCTGAGTACCTTCTCCTCACTTACTCCGT7130.14571747976202884No Hit
CGCATGAAGCGGGGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAGAC6980.14265189463379543No Hit
ATATTGAAGCGGGGTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAA6970.14244752229191318No Hit
GGCCTGAAGCGGGGGTGCTGCTATCACATCCTCAACAGAGAGGCAAGGTAACAAAGACA6950.14203877760814873No Hit
GGGATGAAGCGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATGCACGCTC6950.14203877760814873No Hit
AGACTGAAGCGGGGGTAAAGGAGTAGGAGAGCGGCTATGGCGAGTCCTGCTGCCGCTTC6920.14142566058250206No Hit
CTTTTGAAGCGGGCTTTTTCCTCAGCTGCCGTGCCGGCAGTCTACAGCATGGCTTCCGT6830.13958630950556197No Hit
GCACTGAAGCGGGGGGCCATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTC6810.13917756482179755No Hit
ACATTGAAGCGGGGGAAGTGGGCAACCAGGCCCGTGACCGCCTAGAGGAGGTGCGTGAG6770.13836007545426862No Hit
AGGTTGAAGCGGGGAGACCTTCTGGCCGGAAACCCGCGCGCTCCCGCTACCAGGGCCGT6750.13795133077050417No Hit
ACTGTGAAGCGGGGAGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGG6710.13713384140297524No Hit
AGACTGAAGCGGGGCTGTGAGCGAGTACAACAAGGGCAGCAACGATGCGTACCACAGCC6700.13692946906109302No Hit
CTCTTGAAGCGGGAAAGAAGGGCAGACGGTGGATGCCTTGGCTCTCGGAGGCGACGAAG6630.1354988626679174No Hit
CCGATGAAGCGGGGGACCCCACACCATTCTGCCTTCCAGGAAAACTGCCTGAGCTGGCA6560.13406825627474184No Hit
ACTTTGAAGCGGTCGCCCCCCGGGCCGCGGTTTTCCGCGCGGCGCCTCGCCTCGGCCGG6530.13345513924909513No Hit
TATTTGAAGCGGGGTGCCTAGAGGAGGTGCGTGAGCACATGGAGGAGGTGCGCTCCAAG6480.132433277539684No Hit
CGTTTGAAGCGGGGGCGGCGGGCTGGAGGGTCAGGGGCTGCGCACGCTGGCGCCAGGCT6400.13079829880462615No Hit
CGTCTGAAGCGGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGC6350.12977643709521502No Hit
AACCTGAAGCGGGAGTCCACTAAGGGAAGCCTAGGATGGAACTCAAACCAGCCCCACAG6340.1295720647533328No Hit
GTCTTGAAGCGGGGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACGGCGGCTGCTG6320.12916332006956835No Hit
AGCATGAAGCGGGAGGTGGTTGGCAATCAAGATGAAATACTAGAAGAGGCGCAGGTGTA6300.1287545753858039No Hit
CGAATGAAGCGGGGGCCGGGCAGTGGTGGCACACGCCTTTCATCCCAGCACTTGGGAGG6270.1281414583601572No Hit
AGTTTGAAGCGGGCGTATGAAGCGGGACCTCAACTGGTCTTTCAGACATGACAGCGACC6260.12793708601827497No Hit
CTTCTGAAGCGGGGCATTGCGGGAGTAGCAGCAGCGGCCAGGCAGCATCCAGCAGTGTG6240.12752834133451052No Hit
TTTATGAAGCAGGTCTCCCTCTGGGTGCTCCATCCCCCACCCAGAGGCGAGTAGGTGGA6170.12609773494133492No Hit
GTATTGAAGCGGGGACGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACGGCGGCTG6170.12609773494133492No Hit
GGTTTGAAGCGGGGGAGGAAAAGGAGCTTGGTGGAGAGAGAGAGAGAGAGAGAGAGAGA6160.1258933625994527No Hit
GGGCTGAAGCGGGGCCTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGAC6140.1254846179156882No Hit
GGTTTGAAGCGGGTGATGCCGGTGCCATCCTTCCTGTAGACCGCGACTCGGACGGTCGG6100.12466712854815933No Hit
AGTTTGAAGCGGGGTGGAGAGAGGGGAAGAAAGAAAGCACTGAGTCATCCATCCAGAAG6090.1244627562062771No Hit
TGGTTGAAGCGGGACCTCCGAAGCCTCCTTCTGTTGCCATTTTGCTGAGCATGTTGGTC6050.12364526683874817No Hit
TTTGTGAAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGCTT6030.12323652215498374No Hit
CTTATGAAGCGGGAGAACTGCCTCCACGTACGTCAGTCACCTGGAGACGCACCTAGGCT5950.1216015434199259No Hit
ATACTGAAGCGGGGCCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACGCCAGAGCCG5950.1216015434199259No Hit
TGTTTGAAGCGGGCGGCCAGCGCTCAGGGTCCTTGGTGAAAAGATGCCTAGAGAAAGGC5890.12037530936863253No Hit
CCGGTGAAGCGGGGAGTTCCACGACTCAAGGAACACTCAGTGCTTCGCACCCTCTGTGG5890.12037530936863253No Hit
AGTATGAAGCGGGCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTGTC5890.12037530936863253No Hit
TTTCTGAAGCGGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTT5810.1187403306335747No Hit
GGCATGAAGCGGGGAGACTTTGGCCTTAGCTGTAGCTAGTGTGGGAGCCTGGGAAGTCT5780.11812721360792801No Hit
GGTTTGAAGCGGGGATTGCGGGAGTAGCAGCAGCGGCCAGGCAGCATCCAGCAGTGTGC5750.11751409658228133No Hit
TGTTTGAAGCGGAAAGAAAACCAACTACCTCGTCATGGGCCGCGACAGTGGGCAGTCCA5720.11690097955663464No Hit
CTTGTGAAGCGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGT5680.11608349018910571No Hit
TGTCTGAAGCGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT5670.1158791178472235No Hit
CGTATGAAGCGGGGAATACAGGCTGCCCTTGGACTCCTGATCTCCCTCCCCATGCTTCC5650.11547037316345904No Hit
GCACTGAAGCGGGGAGTAATCTGAAGCGGGGCCTTTTCCGGTCGCGGCGCTGCGCGGTG5630.11506162847969459No Hit
CAGCTGAAGCGGGGTTGACAGAAAGAAGGAAATAATGAAGGTCAGAACAGAAATACATT5540.11322227740275453No Hit
ACATTGAAGCGGGAACCTTGGAGCTGGAAAAGAATATGGTAATGCGTAAGCAGTGAGGT5540.11322227740275453No Hit
GTTCTGAAGCGGGGAGTGCCAGCGCTCTCCCCGGATAGAGCGGGGCCCGAGCCTGTCCG5520.11281353271899008No Hit
TCTTTGAAGCGGGCTTTCCAGCCTCCGCCTCGGACCCTGCAGCCGCCGCGATGTTGATG5510.11260916037710786No Hit
TGGGTGAAGCGGGGCCGTTCCGCGCATGAGCAGTGAGGCTTGGAGCCCGCCTGCCCGTG5470.11179167100957893No Hit
AAAGTGAAGCGGGGCGCGTGCGCGAGGTCAGGAGCAGGAATCCTGTTCCCTACTGGTGA5440.11117855398393224No Hit
GCTCTGAAGCGGGACTACGAGCCCATGCACGACGTCTGCTGCTCCCGCTGTCCCCCAGG5380.10995231993263888No Hit
GGATTGAAGCGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGCA5340.10913483056510997No Hit
CGTCTGAAGCGGGGAAAGTGGCGCTTTCTCCGCCCCAGGGTTCCAGGACATAGTCTGAG5280.1079085965138166No Hit
CGTGTGAAGCGGGGGAGGAGGAGGAGGAGGACTCCGATGGGTCGAAGTCGTAGCCGCTC5280.1079085965138166No Hit
TCTGTGAAGCGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGCAT5250.10729547948816989No Hit
AGCGTGAAGCGGGGCCTTCTTCCTCCGCTTCCACTGGCGTCCACGGTTCGCCGAGCCGC5240.10709110714628768No Hit
TAAATGAAGCGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGT5230.10688673480440546No Hit
TCCTTGAAGCGGGGAGCGAGGCTGCTGTGGTCTACACGACTCTCTGAGCTTCGCCATGC5210.10647799012064099No Hit
TGCGTGAAGCGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGA5210.10647799012064099No Hit
TTTTTGAAGCGGGGGGCCCTGGAGCCAGGAGCTGATGCAGAGGCCATGGAGGCTGCTGC5200.10627361777875877No Hit
TTTCTGAAGCGGGTATTTGTGGTTCACAAATTTGAAAGCCACTGGACCAGATGATCTGA5170.10566050075311209No Hit
CGCTTGAAGCGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAGGCC5130.10484301138558316No Hit
CGGCTGAAGCGGGGGCCCTCGTCCTACAGCACAGGCCTCCCGGCTCCGGCTCCGGCTCC5130.10484301138558316No Hit
TTTTTGAAGCGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTG5070.1036167773342898No Hit
ACGATGAAGCGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCA5020.10259491562487864No Hit
ATATTGAAGCGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTGTC4990.10198179859923195No Hit
GGTTTGAAGCGGGATTCCATGTATTTTCCTTCAGATGGCAGGATTCCCTTTTTGTCACA4980.10177742625734974No Hit
ATATTGAAGCGGGGGTTCGGCCATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGT4980.10177742625734974No Hit
GGTATGAAGCGGGGCAACGCCGCCTTGTCGAGATGAAACCCGCAGTGGATGAGATGTTC4980.10177742625734974No Hit
TGTCTGAAGCGGGATGGGCCGCCGCCCCGCCCGGTGTTACCGGTATTGTAAGAACAAGC4970.10157305391546752No Hit
TTATTGAAGCGGGAGCGCGTGGTGGGGGAACTGCTTGGGCAAAGCGGTTGGCATCGATC4970.10157305391546752No Hit
CCTTTGAAGCGGGAACTCTAGGAACAGGGGTGTAGAGGAGAAGCCCTAGGCTTGAGATT4920.10055119220605636No Hit
CTTCTGAAGCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGG4910.10034681986417415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGG503.6379788E-1253.00000452
TCGCCCT251.4774721E-553.00000427
TCGCCCC550.053.00000413
TTAGGTA251.4774721E-553.00000440
TTCTAGA750.053.00000451
ACTATTG750.053.00000416
TGTCAAT251.4774721E-553.00000417
TCGTTTA503.6379788E-1253.00000449
GATACTA251.4774721E-553.00000413
TAGAGAC251.4774721E-553.00000438
AACCGCG251.4774721E-553.00000423
CCTTACT251.4774721E-553.00000422
ATCTCGC251.4774721E-553.00000453
CAAACTT251.4774721E-553.00000422
CCGGGTA251.4774721E-553.00000431
CCCCTAC550.053.00000435
ACCGGGT251.4774721E-553.00000430
TCACCTA251.4774721E-553.00000415
GGTAATC251.4774721E-553.00000426
ATTAGGT251.4774721E-553.00000439
Produced by FastQC (version 0.11.5)