FastQCFastQC Report
Fri 17 Jun 2016
SRR1527999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8197463
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAC46800.043.1495741
TCGGGGC1071600.042.8449029
TTTACTT1247900.042.8077553
TTACTTC3416250.042.8074384
CGGGATA79200.042.80555710
TTCGTAC41250.042.7660641
CTATACT161150.042.745892
TCGTACT107400.042.702052
TCGGGGG1546400.042.6788069
TCGGGAT606600.042.6785359
ACTTCGG8730800.042.6622776
TACTTCG8801850.042.63335
TTCGGGC852800.042.6017848
CTACTTC1883800.042.596034
CGTACTT325450.042.586573
CTTCGGG8364000.042.5722737
TTCGGGG4468300.042.5687648
TCTACTT704800.042.5114943
TCGGGAG886850.042.4910629
CGGGATT204550.042.4355910