##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527999_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8197463
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91433447153101	33.0	31.0	34.0	30.0	34.0
2	32.409996361069275	34.0	31.0	34.0	30.0	34.0
3	32.58101841996725	34.0	31.0	34.0	31.0	34.0
4	36.1556986594511	37.0	35.0	37.0	35.0	37.0
5	35.93521434619467	37.0	35.0	37.0	35.0	37.0
6	36.15260880104003	37.0	35.0	37.0	35.0	37.0
7	36.125239845547334	37.0	36.0	37.0	35.0	37.0
8	36.13437913656945	37.0	37.0	37.0	35.0	37.0
9	38.1582844594724	39.0	39.0	39.0	37.0	39.0
10	37.8120134241533	39.0	38.0	39.0	35.0	39.0
11	38.18392592927837	39.0	39.0	39.0	37.0	39.0
12	38.096678447953956	39.0	39.0	39.0	37.0	39.0
13	38.05155851267642	39.0	39.0	39.0	37.0	39.0
14	38.65195573313353	40.0	39.0	40.0	36.0	40.0
15	38.18019623876314	40.0	38.0	40.0	34.0	40.0
16	37.72108614579901	40.0	37.0	40.0	33.0	40.0
17	37.72860481346485	40.0	37.0	40.0	33.0	40.0
18	37.75965881151278	40.0	37.0	40.0	33.0	40.0
19	37.850553884781185	40.0	38.0	40.0	33.0	40.0
20	37.84755661599205	40.0	37.0	40.0	33.0	40.0
21	37.66967206805325	40.0	37.0	40.0	33.0	40.0
22	37.72845952461146	40.0	37.0	40.0	33.0	40.0
23	37.673493860234565	40.0	37.0	40.0	33.0	40.0
24	37.65808006696706	40.0	37.0	40.0	33.0	40.0
25	37.751172039446836	40.0	37.0	40.0	33.0	40.0
26	37.73233486994696	40.0	37.0	40.0	33.0	40.0
27	37.69991618138441	40.0	37.0	40.0	33.0	40.0
28	37.65088137634778	40.0	37.0	40.0	33.0	40.0
29	37.654403685628104	40.0	37.0	40.0	33.0	40.0
30	37.612648815859245	40.0	37.0	40.0	33.0	40.0
31	37.559418322473675	40.0	37.0	40.0	33.0	40.0
32	37.49741840859788	40.0	36.0	40.0	33.0	40.0
33	37.42971014324798	40.0	36.0	40.0	33.0	40.0
34	37.33813668936352	40.0	36.0	40.0	33.0	40.0
35	37.23643327209894	40.0	35.0	40.0	33.0	40.0
36	37.14662414456765	39.0	35.0	40.0	33.0	40.0
37	37.004090777841874	39.0	35.0	40.0	32.0	40.0
38	36.82575950632531	39.0	35.0	40.0	32.0	40.0
39	36.708705120108505	39.0	35.0	40.0	32.0	40.0
40	36.57613349398466	39.0	35.0	40.0	31.0	40.0
41	36.420169020586	38.0	35.0	40.0	31.0	40.0
42	36.2746093516982	38.0	35.0	40.0	31.0	40.0
43	36.10182174655744	38.0	35.0	40.0	31.0	40.0
44	35.960718578418714	38.0	35.0	40.0	31.0	40.0
45	35.81221446196219	37.0	35.0	40.0	30.0	40.0
46	35.64685342282118	37.0	35.0	40.0	30.0	40.0
47	35.498756017563	37.0	35.0	40.0	30.0	40.0
48	35.331433883873586	36.0	35.0	40.0	30.0	40.0
49	35.18439461086924	36.0	34.0	40.0	29.0	40.0
50	35.01158370583679	36.0	34.0	40.0	29.0	40.0
51	34.82713907949325	35.0	34.0	39.0	29.0	40.0
52	34.20775598499194	35.0	33.0	38.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	9.0
14	18.0
15	29.0
16	104.0
17	328.0
18	785.0
19	1733.0
20	3358.0
21	5910.0
22	9400.0
23	15082.0
24	22776.0
25	32248.0
26	44966.0
27	61452.0
28	81963.0
29	107626.0
30	136368.0
31	169847.0
32	211059.0
33	269985.0
34	389851.0
35	714771.0
36	765037.0
37	1411592.0
38	2492108.0
39	1249053.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.251489149752796	18.024491235886032	34.44477882974281	18.279240784618363
2	19.817936354211053	23.017182267245367	35.40830864378406	21.756572734759523
3	19.550524351253554	22.191268200905572	37.438570933470515	20.81963651437036
4	19.881699984495203	19.939815525852328	38.948794279400836	21.229690210251633
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	0.0	100.0
11	99.53097684002965	0.3374702636657219	0.0923578429082266	0.03919505339639837
12	99.20399762707072	0.591317094081425	0.09862807553995669	0.10605720330790148
13	95.37938750074261	2.4838660448970615	0.8697568991772211	1.2669895551831096
14	52.541682713298	29.93182158919168	7.010046888897212	10.516448808613104
15	33.157539106916374	26.0701268185047	20.0263300974948	20.74600397708413
16	28.740123621174014	18.299210377649768	26.636131203031958	26.324534798144256
17	22.676674478433146	18.642889879466367	30.075890552967422	28.604545089133065
18	23.720131460184692	20.618122948526878	30.40407745664726	25.25766813464117
19	25.936683093293624	20.008104946615802	28.878446904853366	25.17676505523721
20	28.437956963026245	20.24416578641465	25.646605541250995	25.67127170930811
21	29.962745790984357	20.654878222689142	22.741926374050117	26.640449612276385
22	28.936977208680297	20.63567716011649	24.059736530680283	26.36760910052293
23	28.17383866203483	21.73861595959628	22.975803611434415	27.11174176693448
24	27.70357121465507	23.220489070825938	23.06350147600544	26.012438238513553
25	29.10378491491819	20.163421292660914	24.768077635727053	25.964716156693846
26	29.599438265229132	20.247471687276906	22.836675200607797	27.31641484688617
27	29.46041964446805	22.322991881756586	22.15060927996869	26.065979193806672
28	28.777342453390766	22.280137159508982	22.333446335774862	26.609074051325393
29	29.965588133792124	21.499127229973467	20.87855718287475	27.656727453359654
30	29.01409863027134	21.125816609358285	21.508361794374675	28.3517229659957
31	27.27025422377631	21.550277201617135	22.033109512052693	29.146359062553866
32	28.074429857139947	23.254170223153185	21.976018677973904	26.695381241732964
33	29.352215435434108	22.04056303761298	21.886527575665788	26.72069395128712
34	28.208190753651465	22.369286692724323	22.60121210672131	26.821310446902903
35	30.247468027608054	22.74406118087999	21.59297333821452	25.41549745329744
36	31.567205609833188	21.862837319302326	21.916878429338443	24.653078641526044
37	30.437746410078336	22.42229089658593	22.11809922167383	25.021863471661902
38	28.766204861186928	24.286135844711957	23.280495441089517	23.667163853011598
39	28.659549911966664	24.42611817827052	24.735909146524968	22.178422763237844
40	29.973468620718386	24.296212132948938	22.197940997110933	23.532378249221743
41	29.963746100470352	25.08327271498511	21.32059638451555	23.632384800028987
42	30.37989924443697	24.56172110810381	20.496939113967333	24.561440533491886
43	29.73140592400356	23.20761923536587	21.376552721250462	25.684422119380105
44	28.460866490034782	24.105470192424168	22.6305504520118	24.80311286552925
45	27.278366489729812	23.422246614592833	22.216568711563568	27.082818184113794
46	27.923078152350307	22.993296828543173	21.157362954855667	27.926262064250857
47	28.94152739695196	22.22289993867615	22.56611588243826	26.26945678193363
48	28.64365475025627	21.987668623816905	22.64293233162504	26.725744294301784
49	28.56570380372562	22.309048543433498	23.18546603991991	25.939781612920974
50	27.988281252382595	22.8899721779775	22.328164213732958	26.793582355906942
51	27.044733230268925	23.600191913034557	22.997468851033545	26.35760600566297
52	27.467388385894516	23.217951700422436	23.046349827989467	26.268310085693585
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	4.0
6	8.0
7	47.0
8	86.0
9	343.5
10	601.0
11	1281.0
12	1961.0
13	2768.5
14	3929.0
15	4282.0
16	5206.0
17	6130.0
18	6940.0
19	7750.0
20	9494.5
21	11239.0
22	15590.0
23	19941.0
24	25445.0
25	30949.0
26	39513.5
27	48078.0
28	53589.0
29	59100.0
30	64985.5
31	70871.0
32	74872.5
33	78874.0
34	91021.0
35	103168.0
36	113787.0
37	124406.0
38	143098.0
39	176184.0
40	190578.0
41	208793.5
42	227009.0
43	257474.5
44	287940.0
45	340713.0
46	393486.0
47	431245.0
48	469004.0
49	480611.5
50	492219.0
51	522798.5
52	553378.0
53	594229.0
54	635080.0
55	669390.5
56	703701.0
57	712876.5
58	722052.0
59	700662.0
60	679272.0
61	635148.5
62	591025.0
63	511563.5
64	397336.0
65	362570.0
66	309480.5
67	256391.0
68	223338.5
69	190286.0
70	160733.5
71	131181.0
72	106548.0
73	81915.0
74	56114.0
75	30313.0
76	26501.5
77	22690.0
78	15345.0
79	8000.0
80	5547.0
81	3094.0
82	1940.0
83	786.0
84	537.0
85	288.0
86	205.5
87	123.0
88	122.0
89	85.0
90	49.0
91	24.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	8197463.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.8928262447230315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.46643111913986	4.857128657899749
2	8.557357596298363	1.1796875805049072
3	4.4002797758209065	0.9099109176870698
4	2.6851928089674457	0.7403426986316948
5	1.7965564331446149	0.6191675666252601
6	1.1930464173782664	0.4934076994126621
7	0.8439063247143099	0.4071829764154987
8	0.6085744638537086	0.3355838429055273
9	0.4606766877468744	0.285782792727036
>10	3.265505785130471	4.7663476102274975
>50	1.2621136425526354	6.520070021456122
>100	4.135565326722819	63.50829666710829
>500	0.29929848650584784	13.165299046530887
>1k	0.025495132023893453	2.211791921867788
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	3.903646774617952E-4	0.0	0.0	0.0	0.0
12	0.0016590498792126295	0.0	0.0	0.0	0.0
13	0.0038426522937645463	0.0	0.0	0.0	0.0
14	0.019835405173527467	0.0	0.0	0.0	0.0
15	0.03329078764978872	0.0	0.0	0.0	0.0
16	0.04322068913272314	0.0	0.0	0.0	0.0
17	0.05401671224377591	0.0	0.0	0.0	0.0
18	0.06672796205362562	0.0	0.0	0.0	0.0
19	0.07997596329498528	0.0	0.0	0.0	0.0
20	0.09482201993470418	0.0	0.0	0.0	0.0
21	0.10796023111052773	0.0	0.0	0.0	0.0
22	0.12240372417661416	0.0	0.0	0.0	0.0
23	0.13610308457628903	0.0	0.0	0.0	0.0
24	0.1513639036858111	0.0	0.0	0.0	0.0
25	0.16757623669664626	0.0	0.0	0.0	0.0
26	0.186789498165469	0.0	0.0	0.0	0.0
27	0.205112240213832	0.0	0.0	0.0	0.0
28	0.22452068402138564	0.0	0.0	0.0	0.0
29	0.2474668077184368	0.0	0.0	0.0	0.0
30	0.2764635839161458	0.0	0.0	0.0	0.0
31	0.3037891113384714	0.0	0.0	0.0	0.0
32	0.3261984836040126	0.0	0.0	0.0	0.0
33	0.34438703779449814	0.0	0.0	0.0	0.0
34	0.3624658019194475	0.0	0.0	0.0	0.0
35	0.37909289740008584	0.0	0.0	0.0	0.0
36	0.3968178935360855	0.0	0.0	0.0	0.0
37	0.4141037294099406	0.0	0.0	0.0	0.0
38	0.4338049467255906	0.0	0.0	0.0	0.0
39	0.4551042194395998	0.0	0.0	0.0	0.0
40	0.48131964731039345	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGTAC	4680	0.0	43.149574	1
TCGGGGC	107160	0.0	42.844902	9
TTTACTT	124790	0.0	42.807755	3
TTACTTC	341625	0.0	42.807438	4
CGGGATA	7920	0.0	42.805557	10
TTCGTAC	4125	0.0	42.766064	1
CTATACT	16115	0.0	42.74589	2
TCGTACT	10740	0.0	42.70205	2
TCGGGGG	154640	0.0	42.678806	9
TCGGGAT	60660	0.0	42.678535	9
ACTTCGG	873080	0.0	42.662277	6
TACTTCG	880185	0.0	42.6333	5
TTCGGGC	85280	0.0	42.601784	8
CTACTTC	188380	0.0	42.59603	4
CGTACTT	32545	0.0	42.58657	3
CTTCGGG	836400	0.0	42.572273	7
TTCGGGG	446830	0.0	42.568764	8
TCTACTT	70480	0.0	42.511494	3
TCGGGAG	88685	0.0	42.491062	9
CGGGATT	20455	0.0	42.43559	10
>>END_MODULE