FastQCFastQC Report
Fri 17 Jun 2016
SRR1527998_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527998_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8131723
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT351.0205986E-746.00000422
TCGGGGC1056100.042.868299
TTACTTC3393950.042.8427054
TTAGTAC46450.042.7814871
CTTACTT787750.042.7620433
TTCGGGC852200.042.747838
TCTACTT698300.042.7392233
CGGGATT189650.042.73767510
TCGGGAT590000.042.737129
TCGGGGG1545200.042.710469
ACTTCGG8655100.042.7013786
TACTTCG8727450.042.6586235
TTCGGGG4413400.042.6459438
CTTCGGG8286250.042.6208847
CTACTTC1872550.042.6136554
TCGGGAG872000.042.6001179
ACGTTAC22850.042.577681
TTTACTT1232400.042.5716483
CTATACT162050.042.5510672
CGGGATA77050.042.5373110