FastQCFastQC Report
Fri 17 Jun 2016
SRR1527997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8267254
Sequences flagged as poor quality0
Sequence length59
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTTC3428900.049.153564
ACTTCGG8813650.049.1249666
TCGGGGG1572800.049.124759
TCGTACT117500.049.09832
CTATACT167050.049.0817152
TACTTCG8884550.049.0789345
TTAGTAC47250.049.0740741
CGTACTT336000.049.0644343
TTCGGGG4493250.049.011958
TTCGGGC868450.049.0026478
TCGGGGC1079300.048.9757739
CTTCGGG8448800.048.96397
CTACTTC1903000.048.8892254
CGGGATA84350.048.8844110
TCGGGAT607150.048.8099339
TTTACTT1262250.048.7465633
CTTACTT795050.048.6369443
GTACTTC1798700.048.6154984
TCTACTT702600.048.5908783
ACGTTAC25200.048.5833361
Produced by FastQC (version 0.11.5)