##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527997_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8267254
Sequences flagged as poor quality	0
Sequence length	59
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.489526510253587	31.0	31.0	34.0	30.0	34.0
2	31.985436276664537	33.0	31.0	34.0	30.0	34.0
3	32.241167744452994	34.0	31.0	34.0	30.0	34.0
4	35.947727746117394	37.0	35.0	37.0	35.0	37.0
5	35.608860088246956	37.0	35.0	37.0	33.0	37.0
6	35.89296494337781	37.0	35.0	37.0	35.0	37.0
7	35.887384976922206	37.0	35.0	37.0	35.0	37.0
8	35.83768576603549	37.0	35.0	37.0	35.0	37.0
9	37.91176296264757	39.0	38.0	39.0	35.0	39.0
10	37.49672188613051	39.0	37.0	39.0	34.0	39.0
11	37.86631147416058	39.0	37.0	39.0	35.0	39.0
12	37.82730166509944	39.0	37.0	39.0	35.0	39.0
13	37.73797490678283	39.0	37.0	39.0	35.0	39.0
14	38.07845495009588	40.0	38.0	40.0	34.0	40.0
15	37.618428440689016	40.0	37.0	40.0	32.0	40.0
16	37.201788768072205	39.0	36.0	40.0	32.0	40.0
17	37.153027353459805	39.0	36.0	40.0	32.0	40.0
18	37.158115137142275	40.0	36.0	40.0	32.0	40.0
19	37.223449043660686	40.0	36.0	40.0	32.0	40.0
20	37.3011440074298	40.0	36.0	40.0	32.0	40.0
21	37.26882807761803	40.0	36.0	40.0	32.0	40.0
22	37.23607367089483	40.0	36.0	40.0	32.0	40.0
23	37.171264848037815	40.0	36.0	40.0	32.0	40.0
24	37.1362590286932	39.0	36.0	40.0	32.0	40.0
25	37.074056996434365	39.0	36.0	40.0	31.0	40.0
26	36.92261832042417	39.0	36.0	40.0	31.0	40.0
27	36.7997880553809	39.0	35.0	40.0	31.0	40.0
28	36.76252550121237	39.0	35.0	40.0	31.0	40.0
29	36.73497705525922	39.0	35.0	40.0	31.0	40.0
30	36.696750335722115	39.0	35.0	40.0	31.0	40.0
31	36.67658112355082	39.0	35.0	40.0	31.0	40.0
32	36.560593638468106	39.0	35.0	40.0	31.0	40.0
33	36.42917914461077	39.0	35.0	40.0	30.0	40.0
34	36.34715202895665	39.0	35.0	40.0	30.0	40.0
35	36.19991462703335	38.0	35.0	40.0	30.0	40.0
36	36.00634817800445	38.0	35.0	40.0	30.0	40.0
37	35.85009194104838	38.0	35.0	40.0	29.0	40.0
38	35.657291042467065	38.0	35.0	40.0	29.0	40.0
39	35.511047440903596	38.0	34.0	40.0	28.0	40.0
40	35.33210096121397	38.0	34.0	40.0	28.0	40.0
41	35.20486149330842	37.0	34.0	40.0	28.0	40.0
42	35.09465754892737	37.0	34.0	40.0	27.0	40.0
43	34.874831715585366	37.0	34.0	40.0	27.0	40.0
44	34.74072588068541	37.0	33.0	40.0	27.0	40.0
45	34.53741738187795	36.0	33.0	40.0	26.0	40.0
46	34.3242064414617	36.0	33.0	39.0	26.0	40.0
47	34.19473672878564	36.0	33.0	39.0	26.0	40.0
48	33.98720941681482	35.0	33.0	39.0	25.0	40.0
49	33.87670996923525	35.0	33.0	39.0	25.0	40.0
50	33.6564428769214	35.0	33.0	39.0	24.0	40.0
51	33.463349982956856	35.0	33.0	38.0	24.0	40.0
52	33.310855817421356	35.0	32.0	38.0	24.0	40.0
53	33.11586059893648	35.0	32.0	38.0	23.0	40.0
54	32.943552720165606	35.0	32.0	38.0	23.0	40.0
55	32.75837769106889	35.0	32.0	37.0	23.0	40.0
56	32.55341459207616	35.0	31.0	37.0	23.0	40.0
57	32.39498254196617	35.0	31.0	37.0	22.0	39.0
58	32.2081763787589	35.0	31.0	36.0	22.0	39.0
59	31.7001277570521	34.0	30.0	36.0	21.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	9.0
14	35.0
15	190.0
16	664.0
17	1700.0
18	3789.0
19	7568.0
20	12904.0
21	19863.0
22	29251.0
23	40980.0
24	55557.0
25	73527.0
26	95798.0
27	120946.0
28	148885.0
29	177719.0
30	216703.0
31	260449.0
32	322123.0
33	406140.0
34	569853.0
35	819579.0
36	1046053.0
37	1532893.0
38	1899662.0
39	404408.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.349527666623043	18.26918587477777	33.97089287446594	18.410393584133256
2	19.456956324312767	23.516708208070057	35.17773858163787	21.848596885979312
3	19.54866755031356	22.376982732113955	37.24864386651239	20.825705851060096
4	19.930837978366213	20.13778698465053	38.698895667170746	21.232479369812516
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	0.0	100.0
11	99.51104683610785	0.3514347085501425	0.08969120822947983	0.0478272471125237
12	99.19429111528447	0.6008040880321326	0.0921950625927303	0.11270973409066662
13	95.31798587535837	2.54019049130461	0.8595478014828141	1.2822758318542045
14	52.253069761737095	30.17967029923116	7.051628025460449	10.515631913571303
15	33.10022892728347	26.248026249102786	20.006062472496914	20.64568235111683
16	28.77269768172116	18.389201541406614	26.583204048164	26.25489672870823
17	22.64829410104008	18.733040015463416	30.01698024519387	28.601685638302634
18	23.60671391008429	20.74838876366929	30.341864420761716	25.303032905484702
19	25.93737896525255	20.142734213803035	28.847039174071583	25.07284764687283
20	28.41107821291084	20.38849901067513	25.671063209138122	25.529359567275904
21	29.6270684316703	20.867920593706206	22.79013079796508	26.71488017665842
22	28.899208854596704	20.75463025570522	24.100505439895763	26.245655449802317
23	28.075646399638863	21.911398875612146	22.996402433020684	27.016552291728306
24	27.668751921738465	23.368134086602396	23.092952024940807	25.87016196671833
25	29.075990649374024	20.31547597303772	24.78342869349363	25.825104684094626
26	29.50245631741809	20.361295298293726	22.932862592585156	27.203385791703028
27	29.374941183614293	22.415689659468548	22.209926052834472	25.999443104082687
28	28.72699931561314	22.39175184408269	22.411818966733087	26.46942987357108
29	29.94067921464612	21.61650047282931	20.896406473056228	27.546413839468343
30	28.93764967182574	21.222379280955924	21.541179211380225	28.298791835838117
31	27.14681319819132	21.687951041542934	22.09251100788726	29.07272475237848
32	27.998849436584383	23.418828065522117	22.03592631846076	26.546396179432737
33	29.28012130751033	22.209454312157337	21.914809923585267	26.595614456747064
34	28.14996370015969	22.50350600090429	22.67113118817929	26.675399110756725
35	30.143684952706185	22.912009235472865	21.636990952497648	25.3073148593233
36	31.504499559345824	21.98127697540199	22.012557011070424	24.501666454181763
37	30.386824936067043	22.528665503684778	22.15428484476224	24.93022471548594
38	28.666422974303195	24.45570197794818	23.342635898207558	23.535239149541066
39	28.530476987884974	24.62440370164023	24.780525673942037	22.06459363653276
40	29.871151896385427	24.514488123867974	22.20105974728731	23.413300232459292
41	29.84520615914305	25.2714988556055	21.371884787863056	23.511410197388397
42	30.264353798734138	24.744854821201816	20.520187235084347	24.4706041449797
43	29.63379376029816	23.383677337118225	21.35730921053109	25.625219692052525
44	28.35363471353366	24.26966680835015	22.63073083275293	24.74596764536326
45	27.107586146500395	23.615350393250285	22.290339694413646	26.98672376583567
46	27.824910181784666	23.179462007578334	21.15350514209434	27.84212266854266
47	28.83885023975313	22.39648134676883	22.556909464738837	26.207758948739208
48	28.52448951005981	22.191552358255837	22.616409269631728	26.667548862052627
49	28.445817680211594	22.521335379317	23.170740853008752	25.862106087462656
50	27.849114107296085	23.140065613080232	22.331852874001452	26.678967405622227
51	26.956822664454243	23.785564106292124	23.011836820303333	26.2457764089503
52	27.343093607623526	23.44774939780488	23.037806749375306	26.17135024519629
53	26.65945669505255	23.927703201087084	23.37389174204639	26.038948361813972
54	26.689950496259097	23.060946234384478	23.683462489479577	26.565640779876848
55	26.748035079120587	23.86412707290716	22.58889106346557	26.798946784506683
56	26.823791793502412	23.74620399953842	23.636784354272894	25.79321985268627
57	27.47966858161126	23.065978134940572	23.14651273566773	26.307840547780437
58	27.136967123545496	22.659712644609687	23.542351547442475	26.66096868440234
59	26.51390655228447	23.488282808294024	22.886946500010765	27.11086413941074
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.0
6	36.0
7	72.0
8	245.5
9	917.5
10	1416.0
11	1960.5
12	2505.0
13	2564.5
14	3115.5
15	3607.0
16	3614.0
17	3621.0
18	4237.5
19	5734.0
20	6614.0
21	9226.0
22	11838.0
23	15249.0
24	18660.0
25	24787.0
26	36059.5
27	41205.0
28	46530.0
29	51855.0
30	61324.0
31	74656.0
32	78519.0
33	88039.5
34	97560.0
35	108211.0
36	128647.0
37	138432.0
38	146099.5
39	153767.0
40	161905.5
41	170044.0
42	202929.0
43	253637.5
44	271461.0
45	291748.5
46	312036.0
47	334325.0
48	385194.5
49	413775.0
50	435590.5
51	457406.0
52	478471.0
53	543093.0
54	586650.0
55	616394.5
56	646139.0
57	655602.5
58	635230.5
59	605395.0
60	564655.5
61	523916.0
62	476402.5
63	428889.0
64	381325.5
65	306817.5
66	279873.0
67	251032.5
68	222192.0
69	194189.0
70	137020.5
71	107855.0
72	89328.5
73	70802.0
74	52864.5
75	29357.5
76	23788.0
77	16667.5
78	9547.0
79	6603.5
80	3660.0
81	2669.0
82	1491.0
83	1304.0
84	820.0
85	336.0
86	286.0
87	149.5
88	63.0
89	56.0
90	49.0
91	83.0
92	58.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	8267254.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.944683846000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.68618195303148	8.611953845247607
2	7.432642929167256	1.6269585399988604
3	3.279049929945728	1.0766449439551335
4	1.9423973367078924	0.8503569901432119
5	1.1655488630992088	0.637828190684279
6	0.804911770131108	0.5285702908805524
7	0.5339325808622697	0.4090606304831461
8	0.3829398011407063	0.3352924044428114
9	0.2784414060452744	0.27427078429211177
>10	1.9616836782078175	4.588721346685432
>50	0.8001105218559278	6.56733954283788
>100	2.5507332015362807	60.87156317316176
>500	0.16746151709252657	11.697515288019964
>1k	0.01396451117650643	1.9239240291672743
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	2.2982238116791862E-4	0.0	0.0	0.0	0.0
12	0.0016087566681754304	0.0	0.0	0.0	0.0
13	0.003556198950703583	0.0	0.0	0.0	0.0
14	0.0193413677625001	0.0	0.0	0.0	0.0
15	0.032465435318668086	0.0	0.0	0.0	0.0
16	0.04124706946224224	0.0	0.0	0.0	0.0
17	0.051649556188790136	0.0	0.0	0.0	0.0
18	0.06363660775391684	0.0	0.0	0.0	0.0
19	0.07690582628766456	0.0	0.0	0.0	0.0
20	0.09057421000975656	0.0	0.0	0.0	0.0
21	0.10425468964664687	0.0	0.0	0.0	0.0
22	0.1172094143956385	0.0	0.0	0.0	0.0
23	0.13069635939575583	0.0	0.0	0.0	0.0
24	0.14535660813130938	0.0	0.0	0.0	0.0
25	0.1599926650372663	0.0	0.0	0.0	0.0
26	0.17799138625715383	0.0	0.0	0.0	0.0
27	0.19511920161156293	0.0	0.0	0.0	0.0
28	0.21377110223055926	0.0	0.0	0.0	0.0
29	0.23455188385405842	0.0	0.0	0.0	0.0
30	0.2596388111457565	0.0	0.0	0.0	0.0
31	0.28467735477826134	0.0	0.0	0.0	0.0
32	0.3065225769040119	0.0	0.0	0.0	0.0
33	0.3225133762673797	0.0	0.0	0.0	0.0
34	0.3388065735007053	0.0	0.0	0.0	0.0
35	0.3543498240165356	0.0	0.0	0.0	0.0
36	0.37050996618707976	0.0	0.0	0.0	0.0
37	0.3863919023172628	0.0	0.0	0.0	0.0
38	0.4042938562187638	0.0	0.0	0.0	0.0
39	0.42486900729069166	0.0	0.0	0.0	0.0
40	0.44887939816534	0.0	0.0	0.0	0.0
41	0.4709423467574602	0.0	0.0	0.0	0.0
42	0.49250936284284963	0.0	0.0	0.0	0.0
43	0.512286183538089	0.0	0.0	0.0	0.0
44	0.5327645672916304	0.0	0.0	0.0	0.0
45	0.554343679291818	0.0	0.0	0.0	0.0
46	0.5767695053278876	0.0	0.0	0.0	0.0
47	0.5962439281531691	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACTTC	342890	0.0	49.15356	4
ACTTCGG	881365	0.0	49.124966	6
TCGGGGG	157280	0.0	49.12475	9
TCGTACT	11750	0.0	49.0983	2
CTATACT	16705	0.0	49.081715	2
TACTTCG	888455	0.0	49.078934	5
TTAGTAC	4725	0.0	49.074074	1
CGTACTT	33600	0.0	49.064434	3
TTCGGGG	449325	0.0	49.01195	8
TTCGGGC	86845	0.0	49.002647	8
TCGGGGC	107930	0.0	48.975773	9
CTTCGGG	844880	0.0	48.9639	7
CTACTTC	190300	0.0	48.889225	4
CGGGATA	8435	0.0	48.88441	10
TCGGGAT	60715	0.0	48.809933	9
TTTACTT	126225	0.0	48.746563	3
CTTACTT	79505	0.0	48.636944	3
GTACTTC	179870	0.0	48.615498	4
TCTACTT	70260	0.0	48.590878	3
ACGTTAC	2520	0.0	48.583336	1
>>END_MODULE