FastQCFastQC Report
Fri 17 Jun 2016
SRR1527996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054513
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATCG750.046.00000438
TAAACGG750.046.00000425
CGATTCA551.8189894E-1246.00000423
TATAGCG750.046.00000442
TTCGCGA750.046.00000437
ATATTCG750.046.00000418
CGGCGTA253.418495E-546.029
CGAACTA253.418495E-546.032
GCGACTA501.6370905E-1146.034
CGTATGA900.046.045
CGTATAA206.3131057E-446.024
TAGACGT405.6170393E-946.033
ACGCAAT206.3131057E-446.030
GACGTAA253.418495E-546.039
GGCGTAA206.3131057E-446.030
TAGCGAT206.3131057E-446.020
TACGGTA253.418495E-546.031
TAACGTT206.3131057E-446.046
CGATTGG405.6170393E-946.021
TACGGAT1150.046.017
Produced by FastQC (version 0.11.5)