##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527996_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3054513
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.868460209532582	33.0	31.0	34.0	30.0	34.0
2	32.347608276671274	34.0	31.0	34.0	30.0	34.0
3	32.44972668310791	34.0	31.0	34.0	30.0	34.0
4	35.994727473741314	37.0	35.0	37.0	35.0	37.0
5	36.005933188040125	37.0	35.0	37.0	35.0	37.0
6	36.09298863681379	37.0	36.0	37.0	35.0	37.0
7	36.33109238690423	37.0	37.0	37.0	35.0	37.0
8	36.12046535732537	37.0	37.0	37.0	35.0	37.0
9	38.1357273647223	39.0	39.0	39.0	37.0	39.0
10	37.66766093318313	39.0	38.0	39.0	35.0	39.0
11	38.09094248412104	39.0	38.0	39.0	37.0	39.0
12	38.109877417447564	39.0	39.0	39.0	37.0	39.0
13	38.01156518240387	39.0	39.0	39.0	37.0	39.0
14	38.50313323269536	40.0	38.0	40.0	36.0	40.0
15	37.0800687376351	39.0	35.0	40.0	33.0	40.0
16	36.820102255253126	39.0	35.0	40.0	32.0	40.0
17	36.85199211789244	39.0	35.0	40.0	32.0	40.0
18	37.01356615604517	39.0	35.0	40.0	32.0	40.0
19	37.219902812657864	40.0	35.0	40.0	33.0	40.0
20	37.2759716524369	40.0	35.0	40.0	33.0	40.0
21	37.1123331280633	40.0	35.0	40.0	32.0	40.0
22	37.20455208408018	40.0	35.0	40.0	33.0	40.0
23	37.135343015400494	40.0	35.0	40.0	32.0	40.0
24	37.13911088281503	40.0	35.0	40.0	32.0	40.0
25	37.243350085594656	40.0	35.0	40.0	33.0	40.0
26	37.23811160731678	40.0	35.0	40.0	33.0	40.0
27	37.201948395701706	40.0	35.0	40.0	33.0	40.0
28	37.12535680810656	40.0	35.0	40.0	33.0	40.0
29	37.07625241732479	40.0	35.0	40.0	33.0	40.0
30	36.99786447135763	40.0	35.0	40.0	33.0	40.0
31	36.93056765513848	39.0	35.0	40.0	32.0	40.0
32	36.854663574848104	39.0	35.0	40.0	32.0	40.0
33	36.79526097940981	39.0	35.0	40.0	32.0	40.0
34	36.71684487838159	39.0	35.0	40.0	32.0	40.0
35	36.65154674411273	39.0	35.0	40.0	32.0	40.0
36	36.55775470590566	39.0	35.0	40.0	32.0	40.0
37	36.43107755638951	39.0	35.0	40.0	31.0	40.0
38	36.23914614211824	38.0	35.0	40.0	31.0	40.0
39	36.068105128378896	38.0	35.0	40.0	31.0	40.0
40	35.87261570011324	38.0	35.0	40.0	30.0	40.0
41	35.642345277299526	38.0	35.0	40.0	30.0	40.0
42	35.4497148972684	37.0	35.0	40.0	30.0	40.0
43	35.2483397517051	37.0	35.0	40.0	29.0	40.0
44	35.05940750620476	37.0	34.0	40.0	28.0	40.0
45	34.87207027765146	36.0	34.0	40.0	28.0	40.0
46	34.69567652846788	36.0	34.0	40.0	27.0	40.0
47	34.5397122225376	35.0	34.0	40.0	27.0	40.0
48	34.330529940452045	35.0	34.0	40.0	26.0	40.0
49	34.176982386390236	35.0	34.0	39.0	26.0	40.0
50	34.00967584685349	35.0	33.0	39.0	25.0	40.0
51	33.788368882371756	35.0	33.0	39.0	24.0	40.0
52	33.19126191311021	35.0	33.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	6.0
14	11.0
15	18.0
16	82.0
17	247.0
18	529.0
19	1265.0
20	2239.0
21	3748.0
22	6020.0
23	9280.0
24	13038.0
25	18147.0
26	24807.0
27	32420.0
28	42159.0
29	53780.0
30	66817.0
31	79046.0
32	93494.0
33	120889.0
34	206081.0
35	371812.0
36	243943.0
37	464103.0
38	812908.0
39	387620.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.261063874994147	20.343210194227364	34.3652163208996	16.03050960987889
2	24.25542140432861	21.677432703674857	34.739809586667334	19.327336305329197
3	24.238495629254157	20.592906299629433	36.430586479743255	18.73801159137316
4	28.64397696130283	20.108671987973207	39.13609141620939	12.11125963451457
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	99.1928009473196	0.5037136852912395	0.1687339356552092	0.13475143173396217
12	98.74673966029937	0.7037128340917194	0.3647389943994345	0.18480851120947922
13	93.61191129322415	3.4041433118798317	1.7464649847618918	1.2374804101341195
14	53.83817322106667	27.886671295882522	7.541758702614787	10.73339678043603
15	31.75969458961216	29.517242192126865	18.28147400256604	20.44158921569494
16	29.437262175672522	19.279603655312645	27.26277478602972	24.02035938298511
17	22.218369998752667	18.61848353567328	31.94224414824884	27.22090231732522
18	21.792606546444556	21.363340080726452	32.258661200656206	24.58539217217278
19	24.809683245741628	21.339113632844253	28.389009966564227	25.46219315484989
20	25.949504880156017	21.03130679096799	25.827194056793996	27.191994272082
21	28.09789973066083	22.61607660533774	22.965559485260005	26.32046417874142
22	28.274032554453033	21.33760766446239	22.894386109995278	27.493973671089307
23	26.72730481094695	21.171361850481567	24.950098428129134	27.15123491044235
24	26.392030415323163	22.86253160487449	24.421863648967935	26.32357433083441
25	27.734306581769335	19.641690835822274	25.5740604148681	27.04994216754029
26	29.056743251706575	20.00967093608703	23.802779690248492	27.1308061219579
27	31.213879266514827	22.592472187874137	21.098125953302542	25.095522592308495
28	31.74073903106649	22.17996780501507	20.932600385069566	25.146692778848873
29	33.25852599088627	21.074979874042114	19.954244751945726	25.712249383125886
30	31.828510796974836	22.923850708770924	19.435635075051245	25.812003419202995
31	27.420377651036354	24.00615089868663	20.52471212268535	28.048759327591664
32	28.33934574840572	26.05105953060275	20.2213904475116	25.388204273479932
33	28.11567670525547	26.60437195716633	20.29312037630876	24.98683096126944
34	26.272960697826463	27.133163289859958	21.298256055875356	25.295619956438227
35	28.10097714431073	27.014257264578674	19.26647553963594	25.618290051474652
36	28.715444982555322	27.238024523058176	20.6178202548164	23.428710239570105
37	29.120746907935896	26.841496500424128	20.85805495016718	23.179701641472796
38	28.48015379211023	27.298525165877507	22.432184770534615	21.789136271477645
39	28.548511661269732	27.84676313376306	22.409595244806617	21.19512996016059
40	28.886634301441834	27.8978023665311	20.39611551825119	22.819447813775877
41	28.513023188966617	27.546878995113133	20.26670045273993	23.673397363180317
42	29.2268849404144	27.86398355482527	19.716105316952326	23.19302618780801
43	29.160720546941526	26.48634986984832	19.875705226987083	24.477224356223072
44	27.262512878485047	28.772671781066244	20.22800361301458	23.73681172743413
45	27.105630259226267	27.553262991514522	21.378465241431286	23.962641507827925
46	28.04054197837757	26.741316864586924	20.98052946574462	24.237611691290887
47	29.34896659467483	25.00405138233165	21.240014365628827	24.406967657364692
48	27.169404746354004	25.777824484623245	21.881196773430002	25.171573995592748
49	27.282450590323236	26.050535715513405	21.230618432463704	25.43639526169966
50	27.81720031965816	27.559123172826567	20.378600451201223	24.245076056314048
51	25.441011382174505	28.306672782207837	22.02616259940619	24.226153236211466
52	26.31856534904255	28.200469272843165	21.385078406934262	24.095886971180022
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.0
8	4.0
9	85.0
10	166.0
11	482.0
12	798.0
13	1499.0
14	2563.5
15	2927.0
16	3318.0
17	3709.0
18	3913.0
19	4117.0
20	4609.5
21	5102.0
22	6723.0
23	8344.0
24	10871.0
25	13398.0
26	17811.5
27	22225.0
28	28669.0
29	35113.0
30	41439.0
31	47765.0
32	50481.5
33	53198.0
34	52692.5
35	52187.0
36	51642.5
37	51098.0
38	49329.5
39	51351.0
40	55141.0
41	62146.5
42	69152.0
43	75633.5
44	82115.0
45	94102.5
46	106090.0
47	118608.5
48	131127.0
49	146125.0
50	161123.0
51	170776.0
52	180429.0
53	189339.5
54	198250.0
55	203123.5
56	207997.0
57	230779.0
58	253561.0
59	260348.5
60	267136.0
61	263337.0
62	259538.0
63	233194.5
64	179317.5
65	151784.0
66	131504.5
67	111225.0
68	102514.0
69	93803.0
70	85803.0
71	77803.0
72	60163.5
73	42524.0
74	33256.0
75	23988.0
76	19601.0
77	15214.0
78	10625.5
79	6037.0
80	3665.0
81	1293.0
82	1304.5
83	1316.0
84	1068.0
85	820.0
86	475.5
87	131.0
88	113.5
89	76.5
90	57.0
91	28.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3054513.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.312955344790533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.0630885031795	9.488743674712898
2	7.356964041787231	1.8117193943951166
3	3.3609794008628997	1.2415076783275716
4	2.143415724728132	1.055671284155973
5	1.4911298186783708	0.9180107435336191
6	1.1081975714094061	0.8187112325981601
7	0.8071322691795956	0.6956728511423469
8	0.6241504387311295	0.6148109184422254
9	0.48027471254427784	0.5322240979910822
>10	3.0640193022517175	7.0250561312093
>50	0.4582414276731864	4.153920898629312
>100	1.8337522643886481	53.622555582384244
>500	0.19099914173634436	15.328988160004482
>1k	0.01765538284957805	2.6924073524736967
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.00435421293017905	0.0	0.0	0.0	0.0
12	0.007202457478491661	0.0	0.0	0.0	0.0
13	0.02006866561052449	0.0	0.0	0.0	0.0
14	0.06950371466744454	0.0	0.0	0.0	0.0
15	0.12394774551622469	0.0	0.0	0.0	0.0
16	0.15239745255626674	0.0	0.0	0.0	0.0
17	0.17128753421576534	0.0	0.0	0.0	0.0
18	0.20088308676374925	0.0	0.0	0.0	0.0
19	0.2276631332065046	0.0	0.0	0.0	0.0
20	0.25473782563701647	0.0	0.0	0.0	0.0
21	0.2807976263319226	0.0	0.0	0.0	0.0
22	0.30964019468897336	0.0	0.0	0.0	0.0
23	0.3407417156188237	0.0	0.0	0.0	0.0
24	0.3723343131949348	0.0	0.0	0.0	0.0
25	0.4074954010672078	0.0	0.0	0.0	0.0
26	0.45035002306423316	0.0	0.0	0.0	0.0
27	0.49133855380546754	0.0	0.0	0.0	0.0
28	0.5331455456238032	0.0	0.0	0.0	0.0
29	0.5797323501324106	0.0	0.0	0.0	0.0
30	0.6340781656519386	0.0	0.0	0.0	0.0
31	0.6878346891959537	0.0	0.0	0.0	0.0
32	0.7390376141794126	0.0	0.0	0.0	0.0
33	0.7838565427614811	0.0	0.0	0.0	0.0
34	0.8261873496691616	0.0	0.0	0.0	0.0
35	0.8661937271178745	0.0	0.0	0.0	0.0
36	0.908557272468639	0.0	0.0	0.0	0.0
37	0.9522630939858497	0.0	0.0	0.0	0.0
38	0.9997338364577266	0.0	0.0	0.0	0.0
39	1.0541123904203387	0.0	0.0	0.0	0.0
40	1.1133362339593906	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATATCG	75	0.0	46.000004	38
TAAACGG	75	0.0	46.000004	25
CGATTCA	55	1.8189894E-12	46.000004	23
TATAGCG	75	0.0	46.000004	42
TTCGCGA	75	0.0	46.000004	37
ATATTCG	75	0.0	46.000004	18
CGGCGTA	25	3.418495E-5	46.0	29
CGAACTA	25	3.418495E-5	46.0	32
GCGACTA	50	1.6370905E-11	46.0	34
CGTATGA	90	0.0	46.0	45
CGTATAA	20	6.3131057E-4	46.0	24
TAGACGT	40	5.6170393E-9	46.0	33
ACGCAAT	20	6.3131057E-4	46.0	30
GACGTAA	25	3.418495E-5	46.0	39
GGCGTAA	20	6.3131057E-4	46.0	30
TAGCGAT	20	6.3131057E-4	46.0	20
TACGGTA	25	3.418495E-5	46.0	31
TAACGTT	20	6.3131057E-4	46.0	46
CGATTGG	40	5.6170393E-9	46.0	21
TACGGAT	115	0.0	46.0	17
>>END_MODULE