FastQCFastQC Report
Fri 17 Jun 2016
SRR1527995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028738
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGT750.046.00000426
ACGTTAT551.8189894E-1246.00000430
ACGTTAA750.046.00000429
TACGGAT551.8189894E-1246.00000417
CGTTAAC750.046.00000430
CACGTTA551.8189894E-1246.00000429
ATACGTT551.8189894E-1246.00000432
ATATTCG750.046.00000418
CTAGACG253.4184886E-546.032
CGGCGTA501.6370905E-1146.029
CCGATCA206.313097E-446.033
CCGATAG405.6170393E-946.033
TAAACGT206.313097E-446.014
CGTCTAT301.8624196E-646.046
TACGGAC650.046.044
TCGATAT453.110472E-1046.019
CCATGCG253.4184886E-546.031
ACGATAT405.6170393E-946.033
ACGTCAA453.110472E-1046.019
TTACGTC206.313097E-446.015
Produced by FastQC (version 0.11.5)