##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527995_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3028738
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.815749662070473	33.0	31.0	34.0	30.0	34.0
2	32.19174751992414	34.0	31.0	34.0	30.0	34.0
3	32.38221199720808	34.0	31.0	34.0	30.0	34.0
4	35.9697362399785	37.0	35.0	37.0	35.0	37.0
5	35.9888451889863	37.0	35.0	37.0	35.0	37.0
6	36.08307255365106	37.0	36.0	37.0	35.0	37.0
7	36.33182665519434	37.0	37.0	37.0	35.0	37.0
8	36.07972759611429	37.0	36.0	37.0	35.0	37.0
9	38.12746034817142	39.0	39.0	39.0	35.0	39.0
10	37.67193233617434	39.0	38.0	39.0	35.0	39.0
11	38.08469897363192	39.0	38.0	39.0	37.0	39.0
12	38.092339449632156	39.0	39.0	39.0	37.0	39.0
13	38.01869161347069	39.0	39.0	39.0	37.0	39.0
14	38.5359773608678	40.0	38.0	40.0	36.0	40.0
15	37.11340300811757	39.0	35.0	40.0	33.0	40.0
16	36.859822473914875	39.0	35.0	40.0	32.0	40.0
17	36.904682412278646	39.0	35.0	40.0	32.0	40.0
18	37.09224502086347	39.0	35.0	40.0	32.0	40.0
19	37.28883416129094	40.0	35.0	40.0	33.0	40.0
20	37.315270914816665	40.0	35.0	40.0	33.0	40.0
21	37.34540524799438	40.0	35.0	40.0	33.0	40.0
22	37.34000861084716	40.0	35.0	40.0	33.0	40.0
23	37.23686234992925	40.0	35.0	40.0	33.0	40.0
24	37.24270735864244	40.0	35.0	40.0	33.0	40.0
25	37.19283279042294	40.0	35.0	40.0	33.0	40.0
26	37.18655096611196	40.0	35.0	40.0	33.0	40.0
27	37.17488009857571	40.0	35.0	40.0	33.0	40.0
28	36.948642966146295	40.0	35.0	40.0	32.0	40.0
29	37.002685937179116	40.0	35.0	40.0	32.0	40.0
30	36.99015893748486	40.0	35.0	40.0	32.0	40.0
31	36.96094644039861	40.0	35.0	40.0	33.0	40.0
32	36.885433801140934	39.0	35.0	40.0	32.0	40.0
33	36.81934587937286	39.0	35.0	40.0	32.0	40.0
34	36.76072509408209	39.0	35.0	40.0	32.0	40.0
35	36.63110344968763	39.0	35.0	40.0	32.0	40.0
36	36.16865341274154	39.0	35.0	40.0	31.0	40.0
37	36.25890024161879	39.0	35.0	40.0	31.0	40.0
38	36.18515203361928	38.0	35.0	40.0	31.0	40.0
39	36.055088290898716	38.0	35.0	40.0	31.0	40.0
40	35.86993691762047	38.0	35.0	40.0	30.0	40.0
41	35.67125053405081	38.0	35.0	40.0	30.0	40.0
42	35.33617962332826	37.0	35.0	40.0	29.0	40.0
43	35.148837568650706	37.0	34.0	40.0	28.0	40.0
44	34.96441323085721	37.0	34.0	40.0	28.0	40.0
45	34.54203367871371	36.0	34.0	40.0	26.0	40.0
46	34.51864406891583	36.0	34.0	40.0	26.0	40.0
47	34.43499437719605	35.0	34.0	40.0	26.0	40.0
48	34.22782327160685	35.0	34.0	40.0	25.0	40.0
49	34.08325843965375	35.0	34.0	39.0	25.0	40.0
50	33.849179097036455	35.0	33.0	39.0	24.0	40.0
51	33.7184091195739	35.0	33.0	39.0	24.0	40.0
52	33.2544016682856	35.0	33.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	3.0
14	11.0
15	32.0
16	82.0
17	261.0
18	574.0
19	1240.0
20	2249.0
21	3858.0
22	6080.0
23	9017.0
24	12937.0
25	18208.0
26	24736.0
27	32629.0
28	42346.0
29	53885.0
30	67335.0
31	79341.0
32	94606.0
33	123399.0
34	208436.0
35	365043.0
36	247313.0
37	466095.0
38	784582.0
39	384436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.272852257276792	20.308788677000123	34.331394792154356	16.086964273568725
2	24.406237845597737	21.694679434140557	34.55746254710708	19.341620173154627
3	24.243595847511408	20.58081616831829	36.46297566841371	18.712612315756598
4	28.631066800759918	20.082786956151374	39.16103010560834	12.125116137480363
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	99.18477596939715	0.5089908734264899	0.171886772642599	0.1343463845337563
12	98.74254557508772	0.7007539113650636	0.36780995913149306	0.18889055441573355
13	93.59310709609085	3.4211278757026857	1.7466680841987652	1.2390969440077022
14	53.79448469956793	27.906045356184656	7.570149679503476	10.72932026474393
15	31.80634970736987	29.525201585610905	18.225511747797267	20.44293695922196
16	29.45642706632267	19.350567794243016	27.138762085066453	24.05424305436786
17	22.182077155567764	18.66506776089579	31.947002348833074	27.205852734703367
18	21.776528706015508	21.38012597986356	32.22292585228567	24.62041946183526
19	24.882541837557426	21.33621330072129	28.319484881161728	25.461759980559563
20	25.950082179442397	21.040974821856494	25.8376261003758	27.17131689832531
21	27.8237008285299	22.731910122301763	22.9886507185501	26.455738330618235
22	28.318362301394178	21.34205731892293	22.869261058566305	27.47031932111658
23	26.668104008996487	21.2185405274408	24.94801465164699	27.165340811915723
24	26.36398394314728	22.843243621600813	24.481912928751182	26.310859506500726
25	27.754893292189685	19.657527326563077	25.559292352128182	27.028287029119056
26	29.085150316732577	20.021903512287956	23.780432642242413	27.112513528737054
27	31.27870419957091	22.588252929107767	21.055865512302482	25.077177359018837
28	31.84042330502011	22.187723071457484	20.906760505530684	25.065093117991715
29	33.25487381212901	21.052431738895873	19.986542249610235	25.706152199364883
30	31.82998991659232	22.94675207957902	19.39490969506111	25.828348308767545
31	27.367339135970166	24.080953849425075	20.52746061230783	28.02424640229693
32	28.35028979066529	26.08290977958476	20.18484266384217	25.38195776590778
33	28.124783325596336	26.62722889863699	20.309449018039857	24.938538757726818
34	26.263480036899857	27.20370662632423	21.272259271023113	25.2605540657528
35	28.102397764349373	27.010821008618112	19.27935001310777	25.60743121392474
36	28.809689052007798	27.08586876778381	20.628822961906906	23.475619218301482
37	29.117110823055675	26.845306527008937	20.823128312848453	23.21445433708693
38	28.393079890040013	27.324516019543456	22.400848142031435	21.881555948385103
39	28.51375721505128	27.82257824876236	22.44763330469654	21.216031231489815
40	28.9035565308059	27.91997855212303	20.322061531898765	22.854403385172304
41	28.4879708974497	27.554347718422655	20.31981637236367	23.637865011763974
42	29.262121715381124	27.89042829059496	19.74650828166715	23.100941712356764
43	29.16515063369628	26.528606964352807	19.82611239400701	24.480130007943902
44	27.4294111937051	28.76429060552613	20.16083926704786	23.64545893372091
45	27.154379150656148	27.580893428219937	21.393200732450282	23.871526688673633
46	28.07449835542064	26.74638743925688	20.97768113319805	24.201433072124427
47	29.32950951848592	25.04102368709344	21.20995609392427	24.419510700496378
48	27.23626143958309	25.852813944289665	21.81905466897434	25.091869947152905
49	27.33861430074176	26.08730104749899	21.242411856027164	25.331672795732086
50	27.953589911045455	27.526217190130016	20.38050171391517	24.13969118490936
51	25.4453174886702	28.347813511766283	22.02947234128538	24.177396658278134
52	26.35533347552677	28.221358202657342	21.351995451570918	24.071312870244967
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	3.5
8	7.0
9	78.5
10	150.0
11	434.0
12	718.0
13	1396.0
14	2465.0
15	2856.0
16	3265.5
17	3675.0
18	3881.0
19	4087.0
20	4581.0
21	5075.0
22	6627.0
23	8179.0
24	10732.5
25	13286.0
26	17661.0
27	22036.0
28	28454.0
29	34872.0
30	41018.5
31	47165.0
32	49574.5
33	51984.0
34	52329.5
35	52675.0
36	51947.0
37	51219.0
38	49473.0
39	51339.0
40	54951.0
41	61462.5
42	67974.0
43	74483.0
44	80992.0
45	93112.0
46	105232.0
47	117507.5
48	129783.0
49	144989.5
50	160196.0
51	169357.0
52	178518.0
53	187397.5
54	196277.0
55	201767.0
56	207257.0
57	229008.0
58	250759.0
59	257607.5
60	264456.0
61	260874.0
62	257292.0
63	231000.0
64	178046.5
65	151385.0
66	131173.5
67	110962.0
68	101952.0
69	92942.0
70	84887.5
71	76833.0
72	59514.0
73	42195.0
74	32787.0
75	23379.0
76	19274.0
77	15169.0
78	10591.0
79	6013.0
80	3714.0
81	1415.0
82	1312.5
83	1210.0
84	995.0
85	780.0
86	456.0
87	132.0
88	115.0
89	71.5
90	45.0
91	22.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3028738.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.930121629312657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.86529428086257	9.938776041244637
2	7.5688755248570665	1.9573296226705912
3	3.5372539935146032	1.3721137310974725
4	2.2136522624260944	1.1449117199269019
5	1.4903966024582957	0.9635504672850055
6	1.0697388363042664	0.8299111959008127
7	0.8021657117853376	0.7260470154174004
8	0.6215633433784905	0.642951170416489
9	0.4704755573674935	0.5474975562342321
>10	2.92195718733037	7.015141660093027
>50	0.46625449018675	4.473523530770532
>100	1.7935945729708234	54.29508622083413
>500	0.16423211879055358	13.780517617466451
>1k	0.014545517767279302	2.3126424506423353
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.004655404330120334	0.0	0.0	0.0	0.0
12	0.007395819645013864	0.0	0.0	0.0	0.0
13	0.01918290720425471	0.0	0.0	0.0	0.0
14	0.07148191755113846	0.0	0.0	0.0	0.0
15	0.12407808136590223	0.0	0.0	0.0	0.0
16	0.1514162004108642	0.0	0.0	0.0	0.0
17	0.16841998218399876	0.0	0.0	0.0	0.0
18	0.19879567001173426	0.0	0.0	0.0	0.0
19	0.22319527142988269	0.0	0.0	0.0	0.0
20	0.25211820897020476	0.0	0.0	0.0	0.0
21	0.27879598697543334	0.0	0.0	0.0	0.0
22	0.30745478809986204	0.0	0.0	0.0	0.0
23	0.3384247828633576	0.0	0.0	0.0	0.0
24	0.36942779467883985	0.0	0.0	0.0	0.0
25	0.40426078452477565	0.0	0.0	0.0	0.0
26	0.44563115066407194	0.0	0.0	0.0	0.0
27	0.4846242890603281	0.0	0.0	0.0	0.0
28	0.5259946551996244	0.0	0.0	0.0	0.0
29	0.5702044878097743	0.0	0.0	0.0	0.0
30	0.6240552996000315	0.0	0.0	0.0	0.0
31	0.6754958665952618	0.0	0.0	0.0	0.0
32	0.7267713483305588	0.0	0.0	0.0	0.0
33	0.7707830786287886	0.0	0.0	0.0	0.0
34	0.813738263263445	0.0	0.0	0.0	0.0
35	0.8514437366322211	0.0	0.0	0.0	0.0
36	0.8927810857195307	0.0	0.0	0.0	0.0
37	0.9347787758465738	0.0	0.0	0.0	0.0
38	0.9790546425606969	0.0	0.0	0.0	0.0
39	1.0302971072440072	0.0	0.0	0.0	0.0
40	1.0871194537130646	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGT	75	0.0	46.000004	26
ACGTTAT	55	1.8189894E-12	46.000004	30
ACGTTAA	75	0.0	46.000004	29
TACGGAT	55	1.8189894E-12	46.000004	17
CGTTAAC	75	0.0	46.000004	30
CACGTTA	55	1.8189894E-12	46.000004	29
ATACGTT	55	1.8189894E-12	46.000004	32
ATATTCG	75	0.0	46.000004	18
CTAGACG	25	3.4184886E-5	46.0	32
CGGCGTA	50	1.6370905E-11	46.0	29
CCGATCA	20	6.313097E-4	46.0	33
CCGATAG	40	5.6170393E-9	46.0	33
TAAACGT	20	6.313097E-4	46.0	14
CGTCTAT	30	1.8624196E-6	46.0	46
TACGGAC	65	0.0	46.0	44
TCGATAT	45	3.110472E-10	46.0	19
CCATGCG	25	3.4184886E-5	46.0	31
ACGATAT	40	5.6170393E-9	46.0	33
ACGTCAA	45	3.110472E-10	46.0	19
TTACGTC	20	6.313097E-4	46.0	15
>>END_MODULE