FastQCFastQC Report
Fri 17 Jun 2016
SRR1527994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3077240
Sequences flagged as poor quality0
Sequence length59
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCA203.1390728E-453.00000415
TATACGA401.6025297E-953.00000430
TTTAGCG307.014369E-753.037
CTAGACG353.3445758E-853.032
CGGCGTA503.6379788E-1253.029
ACTATCG150.00669732953.049
CATATAG251.4793173E-553.051
ACGTTAT251.4793173E-553.030
ACGTTAA600.053.029
ATTACGT251.4793173E-553.017
TTCGTAA150.00669732953.027
TACGGTA251.4793173E-553.031
TAACGTG150.00669732953.035
CTAACGG150.00669732953.037
CCGTAGA353.3445758E-853.037
TTGCGAA307.014369E-753.052
CATAACG307.014369E-753.028
TATAGCG600.053.042
ATGCGAT353.3445758E-853.049
CACGTTA251.4793173E-553.029